2V1C

Crystal structure and mutational study of RecOR provide insight into its role in DNA repair

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2v1ca1	Multi-domain proteins (alpha and beta)	Recombination protein RecR	Recombination protein RecR	Recombination protein RecR	Recombination protein RecR	(Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 ) [TaxId: 243230 ],	SCOPe (2.08)
B	d2v1cb1	Multi-domain proteins (alpha and beta)	Recombination protein RecR	Recombination protein RecR	Recombination protein RecR	Recombination protein RecR	(Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 ) [TaxId: 243230 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	RecR N-terminal domain-like	8055731	3001909	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Toprim domain-like	8055733	3000737	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Glucocorticoid receptor-like (DNA-binding domain)	8041284	3000068	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Toprim domain-like	8055733	3000737	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Glucocorticoid receptor-like (DNA-binding domain)	8041284	3000068	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	RecR N-terminal domain-like	8055731	3001909	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Nucleic acid-binding proteins	8042766	3000135	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	ArfGap/RecO-like zinc finger	8042768	3000151	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF21176	e2v1cA5	A: alpha arrays	X: HhH/H2TH	H: SAM/DNA-glycosylase	T: SAM domain-like	F: PF21176	ECOD (1.6)
A	PF13662,PF21175	e2v1cA6	A: a/b three-layered sandwiches	X: HAD domain-like	H: HAD domain-related	T: Toprim domain	F: PF13662,PF21175	ECOD (1.6)
A	PF02132	e2v1cA4	A: few secondary structure elements	X: RING/U-box-like	H: RING/U-box-like	T: RING/U-box	F: PF02132	ECOD (1.6)
B	PF21176	e2v1cB5	A: alpha arrays	X: HhH/H2TH	H: SAM/DNA-glycosylase	T: SAM domain-like	F: PF21176	ECOD (1.6)
B	PF13662,PF21175	e2v1cB6	A: a/b three-layered sandwiches	X: HAD domain-like	H: HAD domain-related	T: Toprim domain	F: PF13662,PF21175	ECOD (1.6)
B	PF02132	e2v1cB4	A: few secondary structure elements	X: RING/U-box-like	H: RING/U-box-like	T: RING/U-box	F: PF02132	ECOD (1.6)
C	PF11967	e2v1cC1	A: beta barrels	X: OB-fold	H: Nucleic acid-binding proteins (From Topology)	T: Nucleic acid-binding proteins	F: PF11967	ECOD (1.6)
C	PF02565	e2v1cC2	A: few secondary structure elements	X: Glucocorticoid receptor-like	H: LIM domain-like	T: LIM domain-like	F: PF02565	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.8.420	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
A	3.30.60.80	Alpha Beta	2-Layer Sandwich	Wheat Germ Agglutinin (Isolectin 2)	domain 1	CATH (4.3.0)
A	3.40.1360.10	Alpha Beta	3-Layer(aba) Sandwich	Dna Topoisomerase Vi A Subunit	Chain: A, domain 2	CATH (4.3.0)
B	1.10.8.420	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
B	3.30.60.80	Alpha Beta	2-Layer Sandwich	Wheat Germ Agglutinin (Isolectin 2)	domain 1	CATH (4.3.0)
B	3.40.1360.10	Alpha Beta	3-Layer(aba) Sandwich	Dna Topoisomerase Vi A Subunit	Chain: A, domain 2	CATH (4.3.0)
B	6.10.250.240	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
C	2.40.50.140	Mainly Beta	Beta Barrel	OB fold (Dihydrolipoamide Acetyltransferase, E2P)	Nucleic acid-binding proteins	CATH (4.3.0)
C	1.20.1440.120	Mainly Alpha	Up-down Bundle	de novo design (two linked rop proteins)	Recombination protein O, C-terminal domain	CATH (4.3.0)
C	6.20.220.20	Special	Other non-globular	Erythroid Transcription Factor GATA-1	Chain A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF21176	RecR, helix-hairpin-helix (RecR_HhH)	RecR, helix-hairpin-helix	-	Motif
A, B	PF21175	RecR, C-terminal (RecR_C)	RecR, C-terminal	The bacterial protein RecR is an important regulator in the RecFOR homologous recombination pathway during DNA repair. It acts with RecF and RecO forming a complex that facilitates the loading of RecA onto ssDNA. RecR is a zinc metalloprotein consist ...	The bacterial protein RecR is an important regulator in the RecFOR homologous recombination pathway during DNA repair. It acts with RecF and RecO forming a complex that facilitates the loading of RecA onto ssDNA. RecR is a zinc metalloprotein consisting of a N-terminal helix-hairpin-helix (HhH) motif, a middle region containing a zinc finger motif (Pfam:PF02132) and a Toprim domain (Pfam:PF13662) and a C-terminal domain comprising a Walker B motif and a C-terminal helix [1,2]. This is the C-terminal domain of RecR, which has a divergent Walker B motif, suggesting a nucleotide binding site [1,2].	Domain
A, B	PF02132	RecR, Cys4-zinc finger motif (RecR_ZnF)	RecR, Cys4-zinc finger motif	-	Motif
A, B	PF01751	Toprim domain (Toprim)	Toprim domain	-	Family
C	PF11967	Recombination protein O N terminal (RecO_N)	Recombination protein O N terminal	Recombination protein O (RecO) is involved in DNA repair and Pfam:PF00470 pathway recombination. This domain forms a beta barrel structure.	Domain
C	PF02565	Recombination protein O C terminal (RecO_C)	Recombination protein O C terminal	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	RECOMBINATION PROTEIN RECR	DNA binding binding organic cyclic compound binding nucleic acid binding cation binding ion binding metal ion binding small molecule binding	cellular response to stimulus double-strand break repair response to stimulus primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process cellular response to stress DNA damage response response to stress metabolic process cellular process cellular response to stimulus double-strand break repair response to stimulus primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process cellular response to stress DNA damage response response to stress metabolic process cellular process macromolecule metabolic process DNA repair DNA recombination	-
C	HYPOTHETICAL PROTEIN	cation binding ion binding binding metal ion binding small molecule binding	cellular response to stimulus double-strand break repair response to stimulus primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process cellular response to stress DNA damage response response to stress metabolic process cellular process cellular response to stimulus double-strand break repair response to stimulus primary metabolic process DNA metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process cellular response to stress DNA damage response response to stress metabolic process cellular process macromolecule metabolic process DNA repair DNA recombination	intracellular organelle bacterial nucleoid intracellular anatomical structure organelle intracellular non-membrane-bounded organelle nucleoid cellular anatomical entity non-membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR023627	Recombination protein RecR	Homologous Superfamily
A, B	IPR006171	TOPRIM domain	Domain
A, B	IPR015967	Recombination protein RecR, conserved site	Conserved Site
A, B	IPR000093	DNA recombination protein RecR	Family
A, B	IPR003583	Helix-hairpin-helix DNA-binding motif, class 1	Domain
A, B	IPR034137	RecR, TOPRIM domain	Domain
C	IPR022572	DNA replication/recombination mediator RecO, N-terminal	Domain
C	IPR042242	Recombination protein O, C-terminal	Homologous Superfamily
C	IPR037278	ARFGAP/RecO-like zinc finger	Homologous Superfamily
C	IPR012340	Nucleic acid-binding, OB-fold	Homologous Superfamily
C	IPR003717	Recombination protein O, RecO	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.