2UZK

Crystal structure of the human FOXO3a-DBD bound to DNA

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Winged helix DNA-binding domain	8062695	3000034	SCOP2B (2022-06-29)
C	SCOP2 Family	Forkhead DNA-binding domain	8062694	4000182	SCOP2 (2022-06-29)
C	SCOP2 Superfamily	Winged helix DNA-binding domain	8062695	3000034	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00250	e2uzkA1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF00250	ECOD (1.6)
C	PF00250	e2uzkC1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF00250	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
C	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C	PF00250	Forkhead domain (Forkhead)	Forkhead domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C	FORKHEAD BOX PROTEIN O3A	DNA binding sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding binding double-stranded DNA binding organic cyclic compound binding nucleic acid binding transcription regulatory region nucleic acid binding transcription factor binding transcription coregulator binding protein binding DNA-binding transcription factor activity, RNA polymerase II-specific transcription regulator activity DNA binding sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding binding double-stranded DNA binding organic cyclic compound binding nucleic acid binding transcription regulatory region nucleic acid binding transcription factor binding transcription coregulator binding protein binding DNA-binding transcription factor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription factor activity DNA-binding transcription activator activity, RNA polymerase II-specific DNA-binding transcription activator activity DNA-binding transcription repressor activity, RNA polymerase II-specific DNA-binding transcription repressor activity RNA polymerase II transcription regulatory region sequence-specific DNA binding cis-regulatory region sequence-specific DNA binding RNA polymerase II cis-regulatory region sequence-specific DNA binding mitochondrial transcription factor activity kinase binding enzyme binding protein kinase binding protein-containing complex binding chromatin binding chromatin DNA binding beta-catenin binding	multicellular organismal process regulation of cellular process cellular developmental process regulation of developmental process negative regulation of biological process regulation of cell differentiation neurogenesis multicellular organism development neuron differentiation cell differentiation anatomical structure development negative regulation of cellular process negative regulation of cell differentiation system development multicellular organismal process regulation of cellular process cellular developmental process regulation of developmental process negative regulation of biological process regulation of cell differentiation neurogenesis multicellular organism development neuron differentiation cell differentiation anatomical structure development negative regulation of cellular process negative regulation of cell differentiation system development developmental process regulation of biological process negative regulation of developmental process biological regulation nervous system development generation of neurons cellular process negative regulation of neuron differentiation regulation of neuron differentiation response to stimulus response to chemical response to xenobiotic stimulus cellular response to stimulus cell surface receptor signaling pathway signaling canonical Wnt signaling pathway cell communication signal transduction Wnt signaling pathway cellular response to nutrient levels response to nutrient levels cellular response to stress response to stress response to starvation cellular response to glucose starvation cellular response to starvation response to decreased oxygen levels cellular response to oxygen levels cellular response to hypoxia cellular response to decreased oxygen levels cellular response to chemical stimulus response to hypoxia response to oxygen levels response to abiotic stimulus cell population proliferation regulation of cell population proliferation neural precursor cell proliferation regulation of neural precursor cell proliferation negative regulation of signaling regulation of canonical Wnt signaling pathway regulation of signal transduction negative regulation of cell communication negative regulation of Wnt signaling pathway regulation of signaling regulation of Wnt signaling pathway negative regulation of response to stimulus negative regulation of signal transduction negative regulation of canonical Wnt signaling pathway regulation of response to stimulus regulation of cell communication positive regulation of programmed cell death regulation of apoptotic process positive regulation of biological process regulation of programmed cell death cell death positive regulation of apoptotic process apoptotic process programmed cell death positive regulation of cellular process neuron apoptotic process positive regulation of neuron apoptotic process regulation of neuron apoptotic process cellular biosynthetic process organonitrogen compound biosynthetic process regulation of primary metabolic process post-transcriptional regulation of gene expression organic substance metabolic process organic substance biosynthetic process protein metabolic process regulation of biosynthetic process organonitrogen compound metabolic process cellular metabolic process regulation of protein metabolic process regulation of cellular metabolic process primary metabolic process regulation of macromolecule metabolic process gene expression translation macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process regulation of translation regulation of macromolecule biosynthetic process metabolic process macromolecule metabolic process regulation of metabolic process cellular response to endogenous stimulus cellular response to growth factor stimulus response to endogenous stimulus cellular response to nerve growth factor stimulus response to nerve growth factor response to growth factor positive regulation of macromolecule biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription positive regulation of metabolic process regulation of RNA metabolic process regulation of miRNA metabolic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process miRNA metabolic process positive regulation of nucleobase-containing compound metabolic process positive regulation of RNA metabolic process organic cyclic compound metabolic process organic cyclic compound biosynthetic process regulation of DNA-templated transcription positive regulation of DNA-templated transcription RNA metabolic process positive regulation of miRNA transcription positive regulation of cellular metabolic process positive regulation of miRNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process miRNA transcription positive regulation of biosynthetic process regulation of RNA biosynthetic process nucleobase-containing compound biosynthetic process nucleic acid metabolic process positive regulation of RNA biosynthetic process ncRNA metabolic process positive regulation of cellular biosynthetic process regulation of miRNA transcription response to oxidative stress cellular response to oxidative stress cellular response to chemical stress sexual reproduction reproductive process multicellular organismal reproductive process oogenesis cell development cellular process involved in reproduction in multicellular organism developmental maturation developmental process involved in reproduction female gamete generation gamete generation oocyte development anatomical structure maturation germ cell development oocyte maturation oocyte differentiation cell maturation signal transduction in absence of ligand extrinsic apoptotic signaling pathway apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand response to organic substance response to ketone response to lipid response to glucocorticoid response to oxygen-containing compound response to corticosteroid response to hormone response to steroid hormone response to dexamethasone response to organic cyclic compound regulation of hydrogen peroxide-mediated programmed cell death positive regulation of hydrogen peroxide-mediated programmed cell death programmed cell death in response to reactive oxygen species cellular response to reactive oxygen species hydrogen peroxide-mediated programmed cell death cellular response to oxygen-containing compound response to reactive oxygen species ovulation cycle process ovulation sex differentiation development of primary female sexual characteristics ovulation from ovarian follicle ovulation cycle development of primary sexual characteristics reproductive structure development rhythmic process gonad development reproductive system development female gonad development animal organ development female sex differentiation homeostatic process regulation of cell development positive regulation of developmental process positive regulation of cell differentiation regulation of immune system process regulation of multicellular organismal process myeloid cell homeostasis erythrocyte homeostasis regulation of multicellular organismal development hemopoiesis regulation of myeloid cell differentiation erythrocyte differentiation regulation of erythrocyte differentiation positive regulation of erythrocyte differentiation multicellular organismal-level homeostasis myeloid cell differentiation immune system process positive regulation of myeloid cell differentiation regulation of hemopoiesis homeostasis of number of cells negative regulation of locomotion regulation of cell motility regulation of locomotion regulation of cell migration negative regulation of cell motility cell migration cell motility negative regulation of cell migration locomotion regulation of muscle adaptation muscle atrophy system process regulation of system process regulation of muscle atrophy muscle system process regulation of muscle system process positive regulation of response to stimulus muscle adaptation positive regulation of muscle atrophy positive regulation of muscle adaptation positive regulation of multicellular organismal process response to cytokine cytokine-mediated signaling pathway cellular response to tumor necrosis factor tumor necrosis factor-mediated signaling pathway cellular response to cytokine stimulus cellular response to organic substance response to tumor necrosis factor response to immobilization stress response to water-immersion restraint stress negative regulation of cellular metabolic process negative regulation of RNA biosynthetic process transcription by RNA polymerase II negative regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II negative regulation of DNA-templated transcription negative regulation of transcription by RNA polymerase II negative regulation of metabolic process negative regulation of cellular biosynthetic process negative regulation of macromolecule biosynthetic process negative regulation of RNA metabolic process negative regulation of macromolecule metabolic process negative regulation of biosynthetic process developmental growth ovarian follicle development growth antral ovarian follicle growth positive regulation of transcription by RNA polymerase II positive regulation of autophagy catabolic process autophagy regulation of catabolic process regulation of autophagy cellular catabolic process positive regulation of catabolic process regulation of cellular catabolic process positive regulation of cellular catabolic process process utilizing autophagic mechanism neuronal stem cell population maintenance maintenance of cell number stem cell population maintenance initiation of primordial ovarian follicle growth head development brain morphogenesis brain development anatomical structure morphogenesis central nervous system development animal organ morphogenesis response to monosaccharide response to carbohydrate carbohydrate homeostasis cellular response to hexose stimulus intracellular chemical homeostasis cellular response to monosaccharide stimulus cellular response to glucose stimulus intracellular glucose homeostasis response to hexose cellular response to carbohydrate stimulus cellular homeostasis chemical homeostasis response to glucose glucose homeostasis regulation of leukocyte differentiation regulation of leukocyte activation regulation of cell activation mononuclear cell differentiation lymphocyte differentiation positive regulation of T cell differentiation positive regulation of cell activation T cell activation positive regulation of leukocyte activation T cell differentiation positive regulation of immune system process positive regulation of T cell activation positive regulation of cell development positive regulation of regulatory T cell differentiation regulation of leukocyte cell-cell adhesion leukocyte differentiation positive regulation of leukocyte cell-cell adhesion lymphocyte activation leukocyte cell-cell adhesion leukocyte activation positive regulation of cell-cell adhesion regulation of T cell differentiation positive regulation of lymphocyte activation cell adhesion cell-cell adhesion positive regulation of hemopoiesis regulation of cell-cell adhesion regulation of lymphocyte differentiation positive regulation of cell adhesion regulation of cell adhesion regulation of regulatory T cell differentiation cell activation regulation of T cell activation regulatory T cell differentiation positive regulation of leukocyte differentiation positive regulation of lymphocyte differentiation regulation of lymphocyte activation positive regulation of epithelial cell apoptotic process positive regulation of endothelial cell apoptotic process regulation of epithelial cell apoptotic process epithelial cell apoptotic process endothelial cell apoptotic process regulation of endothelial cell apoptotic process reactive oxygen species biosynthetic process positive regulation of reactive oxygen species biosynthetic process reactive oxygen species metabolic process regulation of reactive oxygen species biosynthetic process positive regulation of reactive oxygen species metabolic process regulation of reactive oxygen species metabolic process signal transduction by p53 class mediator DNA damage response, signal transduction by p53 class mediator intracellular signal transduction DNA damage response signal transduction in response to DNA damage response to fatty acid mitochondrial gene expression mitochondrial transcription mitochondrial RNA metabolic process cellular response to mineralocorticoid stimulus response to corticosterone response to mineralocorticoid cellular response to ketone cellular response to steroid hormone stimulus cellular response to hormone stimulus response to alcohol cellular response to organic cyclic compound cellular response to glucocorticoid stimulus cellular response to corticosterone stimulus cellular response to corticosteroid stimulus cellular response to alcohol cellular response to lipid cellular response to nitrogen compound response to organonitrogen compound response to nitrogen compound cellular response to amyloid-beta cellular response to organonitrogen compound response to amyloid-beta	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol intracellular organelle protein-containing complex protein-DNA complex organelle intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle membrane-bounded organelle RNA polymerase II transcription repressor complex intracellular anatomical structure cytoplasm cellular anatomical entity cytosol intracellular organelle protein-containing complex protein-DNA complex organelle intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle membrane-bounded organelle RNA polymerase II transcription repressor complex nucleus nuclear protein-containing complex transcription regulator complex intracellular membrane-bounded organelle transcription repressor complex mitochondrial membrane mitochondrion membrane organelle outer membrane organelle envelope bounding membrane of organelle mitochondrial envelope mitochondrial outer membrane outer membrane organelle membrane membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen mitochondrial matrix
B, D	5'-D(CPTPAPTPGPTPAPAPAPCPAPAP*C)-3'	-	-	-
E, F	5'-D(GPTPTPGPTPTPTPAPCPAPTPAP*G)-3'	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C	IPR001766	Fork head domain	Domain
A, C	IPR032068	FOXO protein, KIX-binding domain	Domain
A, C	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A, C	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
A, C	IPR030456	Fork head domain conserved site 2	Conserved Site
A, C	IPR032067	FOXO protein, transactivation domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, C	Pharos: O43524	salivary gland adenoid cystic carcinoma cancer Diabetes Mellitus, Experimental obesity disorder adenoid cystic carcinoma experimental autoimmune encephalomyelitis non-small cell lung carcinoma ovarian cancer astrocytic tumor chronic obstructive pulmonary disease lung adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm sinusitis attention deficit hyperactivity disorder, inattentive type cystic fibrosis salivary gland adenoid cystic carcinoma cancer Diabetes Mellitus, Experimental obesity disorder adenoid cystic carcinoma experimental autoimmune encephalomyelitis non-small cell lung carcinoma ovarian cancer astrocytic tumor chronic obstructive pulmonary disease lung adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm sinusitis attention deficit hyperactivity disorder, inattentive type cystic fibrosis myopathy osteosarcoma psoriasis spina bifida bipolar disorder dermatomyositis

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.