2QN5

Crystal Structure and Functional Study of the Bowman-Birk Inhibitor from Rice Bran in Complex with Bovine Trypsin

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A [auth B]	d2qn5b1	Small proteins	Knottins (small inhibitors, toxins, lectins)	Bowman-Birk inhibitor, BBI	automated matches	automated matches	(Oryza sativa ) [TaxId: 4530 ],	SCOPe (2.08)
B [auth T]	d2qn5t_	All beta proteins	Trypsin-like serine proteases	Trypsin-like serine proteases	Eukaryotic proteases	Trypsin(ogen)	(Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B [auth T]	SCOP2B Superfamily	Trypsin-like serine proteases	8037496	3000114	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth B]	PF00228	e2qn5B5	A: few secondary structure elements	X: Bowman-Birk inhibitor, BBI-like (From Topology)	H: Bowman-Birk inhibitor, BBI-like (From Topology)	T: Bowman-Birk inhibitor, BBI-like	F: PF00228	ECOD (1.6)
B [auth T]	PF00089	e2qn5T1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: FMN-binding split barrel 2	F: PF00089	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A [auth B]	2.10.69.10	Mainly Beta	Ribbon	Cysteine Protease (Bromelain) Inhibitor, subunit H	Cysteine Protease (Bromelain) Inhibitor, subunit H	CATH (4.3.0)
B [auth T]	2.40.10.10	Mainly Beta	Beta Barrel	Thrombin, subunit H	Trypsin-like serine proteases	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth B]	PF00228	Bowman-Birk serine protease inhibitor family (Bowman-Birk_leg)	Bowman-Birk serine protease inhibitor family	-	Domain
B [auth T]	PF00089	Trypsin (Trypsin)	Trypsin	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B]	Bowman-Birk type bran trypsin inhibitor	enzyme regulator activity molecular function inhibitor activity peptidase regulator activity serine-type endopeptidase inhibitor activity molecular function regulator activity enzyme inhibitor activity peptidase inhibitor activity endopeptidase regulator activity endopeptidase inhibitor activity	-	extracellular region cellular anatomical entity
B [auth T]	Cationic trypsin	peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity endopeptidase activity serine hydrolase activity serine-type peptidase activity serine-type endopeptidase activity protein binding binding serpin family protein binding cation binding ion binding metal ion binding peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity endopeptidase activity serine hydrolase activity serine-type peptidase activity serine-type endopeptidase activity protein binding binding serpin family protein binding cation binding ion binding metal ion binding small molecule binding	organic substance metabolic process organonitrogen compound metabolic process proteolysis metabolic process primary metabolic process protein metabolic process macromolecule metabolic process multicellular organismal process digestion	protein-containing complex serine protease inhibitor complex protease inhibitor complex peptidase inhibitor complex extracellular region extracellular space cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth B]	IPR000877	Proteinase inhibitor I12, Bowman-Birk	Domain
A [auth B]	IPR035995	Bowman-Birk type proteinase inhibitor	Homologous Superfamily
B [auth T]	IPR009003	Peptidase S1, PA clan	Homologous Superfamily
B [auth T]	IPR001314	Peptidase S1A, chymotrypsin family	Family
B [auth T]	IPR033116	Serine proteases, trypsin family, serine active site	Active Site
B [auth T]	IPR001254	Serine proteases, trypsin domain	Domain
B [auth T]	IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	Homologous Superfamily
B [auth T]	IPR018114	Serine proteases, trypsin family, histidine active site	Active Site

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.