2QMH

structure of V267F mutant HprK/P

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2qmha_	Alpha and beta proteins (a/b)	PEP carboxykinase-like	PEP carboxykinase-like	HPr kinase HprK C-terminal domain	HPr kinase HprK C-terminal domain	(Lacticaseibacillus casei ) [TaxId: 1582 ],	SCOPe (2.08)
B	d2qmhb_	Alpha and beta proteins (a/b)	PEP carboxykinase-like	PEP carboxykinase-like	HPr kinase HprK C-terminal domain	HPr kinase HprK C-terminal domain	(Lacticaseibacillus casei ) [TaxId: 1582 ],	SCOPe (2.08)
C	d2qmhc_	Alpha and beta proteins (a/b)	PEP carboxykinase-like	PEP carboxykinase-like	HPr kinase HprK C-terminal domain	HPr kinase HprK C-terminal domain	(Lacticaseibacillus casei ) [TaxId: 1582 ],	SCOPe (2.08)
D	d2qmhd_	Alpha and beta proteins (a/b)	PEP carboxykinase-like	PEP carboxykinase-like	HPr kinase HprK C-terminal domain	HPr kinase HprK C-terminal domain	(Lacticaseibacillus casei ) [TaxId: 1582 ],	SCOPe (2.08)
E	d2qmhe_	Alpha and beta proteins (a/b)	PEP carboxykinase-like	PEP carboxykinase-like	HPr kinase HprK C-terminal domain	HPr kinase HprK C-terminal domain	(Lacticaseibacillus casei ) [TaxId: 1582 ],	SCOPe (2.08)
F	d2qmhf_	Alpha and beta proteins (a/b)	PEP carboxykinase-like	PEP carboxykinase-like	HPr kinase HprK C-terminal domain	HPr kinase HprK C-terminal domain	(Lacticaseibacillus casei ) [TaxId: 1582 ],	SCOPe (2.08)
G	d2qmhg_	Alpha and beta proteins (a/b)	PEP carboxykinase-like	PEP carboxykinase-like	HPr kinase HprK C-terminal domain	HPr kinase HprK C-terminal domain	(Lacticaseibacillus casei ) [TaxId: 1582 ],	SCOPe (2.08)
H	d2qmhh_	Alpha and beta proteins (a/b)	PEP carboxykinase-like	PEP carboxykinase-like	HPr kinase HprK C-terminal domain	HPr kinase HprK C-terminal domain	(Lacticaseibacillus casei ) [TaxId: 1582 ],	SCOPe (2.08)
I	d2qmhi_	Alpha and beta proteins (a/b)	PEP carboxykinase-like	PEP carboxykinase-like	HPr kinase HprK C-terminal domain	HPr kinase HprK C-terminal domain	(Lacticaseibacillus casei ) [TaxId: 1582 ],	SCOPe (2.08)
J	d2qmhj_	Alpha and beta proteins (a/b)	PEP carboxykinase-like	PEP carboxykinase-like	HPr kinase HprK C-terminal domain	HPr kinase HprK C-terminal domain	(Lacticaseibacillus casei ) [TaxId: 1582 ],	SCOPe (2.08)
K	d2qmhk_	Alpha and beta proteins (a/b)	PEP carboxykinase-like	PEP carboxykinase-like	HPr kinase HprK C-terminal domain	HPr kinase HprK C-terminal domain	(Lacticaseibacillus casei ) [TaxId: 1582 ],	SCOPe (2.08)
L	d2qmhl_	Alpha and beta proteins (a/b)	PEP carboxykinase-like	PEP carboxykinase-like	HPr kinase HprK C-terminal domain	HPr kinase HprK C-terminal domain	(Lacticaseibacillus casei ) [TaxId: 1582 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	PEPCK/HprK-like	8057754	3001670	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	PEPCK/HprK-like	8057754	3001670	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	PEPCK/HprK-like	8057754	3001670	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	PEPCK/HprK-like	8057754	3001670	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	PEPCK/HprK-like	8057754	3001670	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	PEPCK/HprK-like	8057754	3001670	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	PEPCK/HprK-like	8057754	3001670	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	PEPCK/HprK-like	8057754	3001670	SCOP2B (2022-06-29)
I	SCOP2B Superfamily	PEPCK/HprK-like	8057754	3001670	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	PEPCK/HprK-like	8057754	3001670	SCOP2B (2022-06-29)
K	SCOP2B Superfamily	PEPCK/HprK-like	8057754	3001670	SCOP2B (2022-06-29)
L	SCOP2B Superfamily	PEPCK/HprK-like	8057754	3001670	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF07475	e2qmhA1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: PEP carboxykinase catalytic C-terminal domain	F: PF07475	ECOD (1.6)
B	PF07475	e2qmhB1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: PEP carboxykinase catalytic C-terminal domain	F: PF07475	ECOD (1.6)
C	PF07475	e2qmhC1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: PEP carboxykinase catalytic C-terminal domain	F: PF07475	ECOD (1.6)
D	PF07475	e2qmhD1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: PEP carboxykinase catalytic C-terminal domain	F: PF07475	ECOD (1.6)
E	PF07475	e2qmhE1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: PEP carboxykinase catalytic C-terminal domain	F: PF07475	ECOD (1.6)
F	PF07475	e2qmhF1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: PEP carboxykinase catalytic C-terminal domain	F: PF07475	ECOD (1.6)
G	PF07475	e2qmhG1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: PEP carboxykinase catalytic C-terminal domain	F: PF07475	ECOD (1.6)
H	PF07475	e2qmhH1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: PEP carboxykinase catalytic C-terminal domain	F: PF07475	ECOD (1.6)
I	PF07475	e2qmhI1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: PEP carboxykinase catalytic C-terminal domain	F: PF07475	ECOD (1.6)
J	PF07475	e2qmhJ1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: PEP carboxykinase catalytic C-terminal domain	F: PF07475	ECOD (1.6)
K	PF07475	e2qmhK1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: PEP carboxykinase catalytic C-terminal domain	F: PF07475	ECOD (1.6)
L	PF07475	e2qmhL1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: PEP carboxykinase catalytic C-terminal domain	F: PF07475	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
B	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
C	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
D	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
E	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
F	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
G	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
H	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
I	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
J	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
K	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
L	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	PF07475	HPr Serine kinase C-terminal domain (Hpr_kinase_C)	HPr Serine kinase C-terminal domain	This family represents the C terminal kinase domain of Hpr Serine/threonine kinase PtsK. This kinase is the sensor in a multicomponent phosphorelay system in control of carbon catabolic repression in bacteria [1]. This kinase in unusual in that i ...	This family represents the C terminal kinase domain of Hpr Serine/threonine kinase PtsK. This kinase is the sensor in a multicomponent phosphorelay system in control of carbon catabolic repression in bacteria [1]. This kinase in unusual in that it recognises the tertiary structure of its target and is a member of a novel family unrelated to any previously described protein phosphorylating enzymes [1]. X-ray analysis of the full-length crystalline enzyme from Staphylococcus xylosus at a resolution of 1.95 A shows the enzyme to consist of two clearly separated domains that are assembled in a hexameric structure resembling a three-bladed propeller [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	HPr kinase/phosphorylase	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding catalytic activity, acting on a protein small molecule sensor activity kinase activity protein kinase activity transferase activity phosphotransferase activity, nitrogenous group as acceptor phosphotransferase activity, alcohol group as acceptor protein histidine kinase activity transferase activity, transferring phosphorus-containing groups phosphorelay sensor kinase activity catalytic activity protein serine/threonine/tyrosine kinase activity protein serine/threonine kinase activity	carbohydrate metabolic process regulation of biological process organic substance metabolic process biological regulation primary metabolic process metabolic process regulation of carbohydrate metabolic process regulation of metabolic process regulation of primary metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR003755	HPr(Ser) kinase/phosphorylase	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR011104	HPr kinase/phosphorylase, C-terminal	Domain
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR028979	HPr(Ser) kinase/phosphorylase-like, N-terminal domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR011126	HPr(Ser) kinase/phosphorylase, N-terminal	Domain
A, B, C, D, E A, B, C, D, E, F, G, H, I, J, K, L	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.