2PSN

Crystal structure of enolase1

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2psna2	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Enolase C-terminal domain-like	Enolase	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d2psna1	Alpha and beta proteins (a+b)	Enolase N-terminal domain-like	Enolase N-terminal domain-like	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d2psnc2	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Enolase C-terminal domain-like	Enolase	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d2psnc1	Alpha and beta proteins (a+b)	Enolase N-terminal domain-like	Enolase N-terminal domain-like	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D	d2psnd2	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Enolase C-terminal domain-like	Enolase	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D	d2psnd1	Alpha and beta proteins (a+b)	Enolase N-terminal domain-like	Enolase N-terminal domain-like	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2psnb2	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Enolase C-terminal domain-like	Enolase	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2psnb1	Alpha and beta proteins (a+b)	Enolase N-terminal domain-like	Enolase N-terminal domain-like	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Enolase_N	e2psnA1	A: a+b two layers	X: Enolase-N/ribosomal protein	H: Enolase N-terminal domain-like (From Topology)	T: Enolase N-terminal domain-like	F: Enolase_N	ECOD (1.6)
A	Enolase_C	e2psnA2	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Enolase_C	ECOD (1.6)
C	Enolase_N	e2psnC1	A: a+b two layers	X: Enolase-N/ribosomal protein	H: Enolase N-terminal domain-like (From Topology)	T: Enolase N-terminal domain-like	F: Enolase_N	ECOD (1.6)
C	Enolase_C	e2psnC2	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Enolase_C	ECOD (1.6)
D	Enolase_N	e2psnD1	A: a+b two layers	X: Enolase-N/ribosomal protein	H: Enolase N-terminal domain-like (From Topology)	T: Enolase N-terminal domain-like	F: Enolase_N	ECOD (1.6)
D	Enolase_C	e2psnD2	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Enolase_C	ECOD (1.6)
B	Enolase_N	e2psnB1	A: a+b two layers	X: Enolase-N/ribosomal protein	H: Enolase N-terminal domain-like (From Topology)	T: Enolase N-terminal domain-like	F: Enolase_N	ECOD (1.6)
B	Enolase_C	e2psnB2	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Enolase_C	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.390.10	Alpha Beta	2-Layer Sandwich	Enolase-like	domain 1	CATH (4.3.0)
A	3.20.20.120	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Enolase-like C-terminal domain	CATH (4.3.0)
C	3.30.390.10	Alpha Beta	2-Layer Sandwich	Enolase-like	domain 1	CATH (4.3.0)
C	3.20.20.120	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Enolase-like C-terminal domain	CATH (4.3.0)
D	3.30.390.10	Alpha Beta	2-Layer Sandwich	Enolase-like	domain 1	CATH (4.3.0)
D	3.20.20.120	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Enolase-like C-terminal domain	CATH (4.3.0)
B	3.30.390.10	Alpha Beta	2-Layer Sandwich	Enolase-like	domain 1	CATH (4.3.0)
B	3.20.20.120	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Enolase-like C-terminal domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF00113	Enolase, C-terminal TIM barrel domain (Enolase_C)	Enolase, C-terminal TIM barrel domain	-	Domain
A, B, C, D	PF03952	Enolase, N-terminal domain (Enolase_N)	Enolase, N-terminal domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Alpha-enolase	transcription factor binding transcription coregulator binding transcription corepressor binding protein binding binding hydro-lyase activity lyase activity phosphopyruvate hydratase activity catalytic activity carbon-oxygen lyase activity DNA-binding transcription factor activity, RNA polymerase II-specific DNA-binding transcription repressor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription repressor activity transcription factor binding transcription coregulator binding transcription corepressor binding protein binding binding hydro-lyase activity lyase activity phosphopyruvate hydratase activity catalytic activity carbon-oxygen lyase activity DNA-binding transcription factor activity, RNA polymerase II-specific DNA-binding transcription repressor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription repressor activity DNA-binding transcription factor activity RNA binding organic cyclic compound binding nucleic acid binding enzyme binding GTPase binding DNA binding sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding double-stranded DNA binding RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription regulatory region nucleic acid binding cation binding magnesium ion binding ion binding metal ion binding small molecule binding transcription corepressor activity transcription coregulator activity protein-macromolecule adaptor activity molecular adaptor activity identical protein binding protein homodimerization activity protein dimerization activity cell adhesion molecule binding cadherin binding	regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process negative regulation of metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process organic substance metabolic process negative regulation of cellular process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process biological regulation regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process cellular process cellular response to stimulus response to stimulus cellular response to oxygen levels cellular response to hypoxia regulation of cellular response to stress regulation of signal transduction regulation of programmed cell death intracellular signal transduction cell communication negative regulation of apoptotic signaling pathway signal transduction intrinsic apoptotic signaling pathway response to decreased oxygen levels negative regulation of programmed cell death regulation of apoptotic process cell death negative regulation of apoptotic process intrinsic apoptotic signaling pathway in response to hypoxia response to hypoxia regulation of response to stimulus response to chemical response to oxygen levels response to abiotic stimulus regulation of apoptotic signaling pathway regulation of cellular response to hypoxia signaling negative regulation of signaling negative regulation of intracellular signal transduction cellular response to decreased oxygen levels regulation of intrinsic apoptotic signaling pathway negative regulation of cell communication cellular response to stress regulation of intracellular signal transduction response to stress regulation of response to stress regulation of signaling negative regulation of response to stimulus regulation of hypoxia-induced intrinsic apoptotic signaling pathway negative regulation of signal transduction negative regulation of cellular response to hypoxia apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway cellular response to chemical stimulus negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway regulation of cell communication apoptotic process programmed cell death regulation of protein maturation positive regulation of protein maturation positive regulation of macromolecule biosynthetic process positive regulation of protein processing protein maturation positive regulation of metabolic process positive regulation of plasminogen activation regulation of proteolysis proteolysis positive regulation of macromolecule metabolic process plasminogen activation zymogen activation regulation of protein metabolic process positive regulation of proteolysis regulation of protein processing protein processing positive regulation of protein metabolic process positive regulation of biological process positive regulation of cellular metabolic process positive regulation of biosynthetic process regulation of plasminogen activation protein metabolic process positive regulation of cellular process organonitrogen compound metabolic process positive regulation of gene expression positive regulation of cellular biosynthetic process regulation of growth regulation of cellular component organization growth negative regulation of cell growth regulation of cell growth cell growth cellular component organization negative regulation of growth cellular component organization or biogenesis multicellular organismal process system process regulation of system process muscle contraction muscle system process regulation of muscle system process regulation of muscle contraction positive regulation of muscle contraction positive regulation of multicellular organismal process regulation of multicellular organismal process ribonucleotide catabolic process pyridine nucleotide catabolic process oxoacid metabolic process carbohydrate catabolic process carbohydrate derivative metabolic process organophosphate catabolic process nucleoside phosphate catabolic process organic acid metabolic process glycolytic process phosphate-containing compound metabolic process pyridine-containing compound metabolic process small molecule metabolic process purine ribonucleotide catabolic process phosphorus metabolic process pyridine nucleotide metabolic process nucleoside phosphate metabolic process organonitrogen compound catabolic process nicotinamide nucleotide metabolic process pyruvate metabolic process purine nucleoside diphosphate metabolic process purine-containing compound catabolic process nucleotide metabolic process nucleobase-containing compound catabolic process nucleoside diphosphate metabolic process purine nucleoside diphosphate catabolic process nucleoside diphosphate catabolic process nucleoside triphosphate metabolic process monocarboxylic acid metabolic process purine ribonucleotide metabolic process pyridine-containing compound catabolic process ribose phosphate metabolic process catabolic process ATP metabolic process purine nucleotide metabolic process ribonucleotide metabolic process nucleobase-containing small molecule metabolic process purine ribonucleoside triphosphate metabolic process ribonucleoside diphosphate metabolic process ribonucleoside diphosphate catabolic process nucleotide catabolic process ribonucleoside triphosphate metabolic process purine nucleoside triphosphate metabolic process purine nucleotide catabolic process carbohydrate metabolic process carbohydrate derivative catabolic process ADP catabolic process carboxylic acid metabolic process generation of precursor metabolites and energy organic cyclic compound catabolic process purine-containing compound metabolic process ADP metabolic process organophosphate metabolic process purine ribonucleoside diphosphate metabolic process organic substance catabolic process purine ribonucleoside diphosphate catabolic process monosaccharide metabolic process small molecule biosynthetic process carbohydrate biosynthetic process hexose metabolic process monosaccharide biosynthetic process gluconeogenesis hexose biosynthetic process glucose metabolic process biological process involved in interspecies interaction between organisms response to other organism response to external stimulus response to external biotic stimulus response to virus response to biotic stimulus nucleotide biosynthetic process organonitrogen compound biosynthetic process purine ribonucleotide biosynthetic process positive regulation of phosphate metabolic process purine ribonucleoside triphosphate biosynthetic process positive regulation of nucleotide biosynthetic process regulation of nucleotide biosynthetic process regulation of ATP metabolic process ribonucleotide biosynthetic process purine nucleoside triphosphate biosynthetic process regulation of small molecule metabolic process positive regulation of purine nucleotide biosynthetic process regulation of phosphate metabolic process regulation of nucleotide metabolic process ribose phosphate biosynthetic process positive regulation of nucleobase-containing compound metabolic process positive regulation of phosphorus metabolic process regulation of purine nucleotide metabolic process ribonucleoside triphosphate biosynthetic process positive regulation of ATP metabolic process organophosphate biosynthetic process nucleoside phosphate biosynthetic process regulation of phosphorus metabolic process purine nucleotide biosynthetic process ATP biosynthetic process positive regulation of nucleotide metabolic process purine-containing compound biosynthetic process carbohydrate derivative biosynthetic process regulation of purine nucleotide biosynthetic process nucleoside triphosphate biosynthetic process positive regulation of purine nucleotide metabolic process positive regulation of ATP biosynthetic process regulation of ATP biosynthetic process positive regulation of small molecule metabolic process NADH regeneration glucose catabolic process to pyruvate hexose catabolic process NADH metabolic process glucose catabolic process monosaccharide catabolic process small molecule catabolic process canonical glycolysis NAD metabolic process glycolytic process through glucose-6-phosphate NAD catabolic process glycolytic process through fructose-6-phosphate transcription by RNA polymerase II regulation of transcription by RNA polymerase II negative regulation of transcription by RNA polymerase II	M band intracellular anatomical structure cellular anatomical entity sarcomere intracellular organelle organelle intracellular non-membrane-bounded organelle contractile muscle fiber A band cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex M band intracellular anatomical structure cellular anatomical entity sarcomere intracellular organelle organelle intracellular non-membrane-bounded organelle contractile muscle fiber A band cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex non-membrane-bounded organelle membrane cell periphery cell cortex extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region extracellular space vesicle extracellular membrane-bounded organelle cell surface cytosol plasma membrane protein-containing complex catalytic complex phosphopyruvate hydratase complex nuclear outer membrane nuclear membrane nucleus organelle outer membrane intracellular membrane-bounded organelle endomembrane system organelle envelope bounding membrane of organelle nuclear outer membrane-endoplasmic reticulum membrane network nuclear envelope outer membrane organelle membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR020811	Enolase, N-terminal	Domain
A, B, C, D	IPR036849	Enolase-like, C-terminal domain superfamily	Homologous Superfamily
A, B, C, D	IPR020809	Enolase, conserved site	Conserved Site
A, B, C, D	IPR020810	Enolase, C-terminal TIM barrel domain	Domain
A, B, C, D	IPR000941	Enolase	Family
A, B, C, D	IPR029017	Enolase-like, N-terminal	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D	Pharos: P06733	Alzheimer disease rheumatoid arthritis non-small cell lung carcinoma squamous cell carcinoma Mammary Neoplasms, Experimental esophageal cancer osteoporosis carcinoma granular cell carcinoma acute coronary syndrome perinatal necrotizing enterocolitis sarcomatoid carcinoma tubular adenocarcinoma adenocarcinoma lung adenocarcinoma Alzheimer disease rheumatoid arthritis non-small cell lung carcinoma squamous cell carcinoma Mammary Neoplasms, Experimental esophageal cancer osteoporosis carcinoma granular cell carcinoma acute coronary syndrome perinatal necrotizing enterocolitis sarcomatoid carcinoma tubular adenocarcinoma adenocarcinoma lung adenocarcinoma male infertility breast carcinoma cribriform carcinoma diabetes mellitus myopathy neoplasm of esophagus osteoarthritis gastric neoplasm osteosarcoma limb-girdle muscular dystrophy pancreatic intraductal papillary-mucinous carcinoma lung cancer Becker muscular dystrophy cribriform carcinoma of breast necrotizing enterocolitis drug-induced liver injury osteoarthritis susceptibility 1 hepatocellular carcinoma ovarian cancer lymphoplasmacytic lymphoma breast cancer malignant pancreatic neoplasm congenital anemia mammary neoplasms, animal Duchenne muscular dystrophy oral cavity neoplasm gastric cancer hereditary diffuse gastric adenocarcinoma pancreatic ductal adenocarcinoma plasma cell myeloma psoriasis undifferentiated carcinoma

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.