Annotations: 2PJW

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth H]	F_UNCLASSIFIED	e2pjwH1	A: alpha bundles	X: Spectrin repeat-like	H: GAT-like domain (From Topology)	T: GAT-like domain	F: F_UNCLASSIFIED	ECOD (1.6)
B [auth V]	PF21356	e2pjwV1	A: alpha bundles	X: Spectrin repeat-like	H: GAT-like domain (From Topology)	T: GAT-like domain	F: PF21356	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A [auth H]	1.20.5.1940	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
B [auth V]	1.20.5.1940	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B [auth V]	PF21356	Vacuolar protein sorting-associated protein 27, GAT-like domain (Vps27_GAT-like)	Vacuolar protein sorting-associated protein 27, GAT-like domain	This domain is found in Vacuolar protein sorting-associated protein 27 from Saccharomyces cerevisiae (Vps27) and similar sequences predominantly found in yeast. Vps27 binds to ubiquitinated transmembrane proteins and recruits both ubiquitin ligases a ...	This domain is found in Vacuolar protein sorting-associated protein 27 from Saccharomyces cerevisiae (Vps27) and similar sequences predominantly found in yeast. Vps27 binds to ubiquitinated transmembrane proteins and recruits both ubiquitin ligases and deubiquitinating enzymes. Besides the GAT domain (this entry), Vps27 contains VHS (Pfam:PF00790) and FYVE domains (Pfam:PF01363). This domain forms a three-helix bundle that resemble closely the structure of a GAT domain [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth H]	Uncharacterized protein YHL002W	binding phospholipid binding lipid binding phosphatidylinositol binding protein binding protein domain specific binding protein heterodimerization activity protein dimerization activity ubiquitin binding ubiquitin-like protein binding	localization cellular localization late endosome to vacuole transport transport establishment of localization in cell cellular process vacuolar transport intracellular transport establishment of localization vesicle-mediated transport multivesicular body sorting pathway regulation of protein maturation positive regulation of protein maturation regulation of cellular process localization cellular localization late endosome to vacuole transport transport establishment of localization in cell cellular process vacuolar transport intracellular transport establishment of localization vesicle-mediated transport multivesicular body sorting pathway regulation of protein maturation positive regulation of protein maturation regulation of cellular process positive regulation of protein metabolic process cellular biosynthetic process positive regulation of biological process positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process regulation of primary metabolic process protein maturation positive regulation of metabolic process organic substance metabolic process positive regulation of biosynthetic process organic substance biosynthetic process positive regulation of macromolecule metabolic process protein metabolic process positive regulation of cellular process regulation of biosynthetic process regulation of biological process organonitrogen compound metabolic process regulation of protein metabolic process cellular metabolic process biological regulation primary metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process positive regulation of gene expression metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of metabolic process positive regulation of cellular biosynthetic process protein localization establishment of protein localization nitrogen compound transport protein targeting organic substance transport establishment of protein localization to vacuole protein localization to vacuole establishment of protein localization to organelle protein transport cellular macromolecule localization macromolecule localization protein localization to organelle protein targeting to vacuole intracellular protein transport catabolic process proteolysis involved in protein catabolic process macromolecule catabolic process modification-dependent macromolecule catabolic process late endosome to vacuole transport via multivesicular body sorting pathway proteolysis endosome transport via multivesicular body sorting pathway organonitrogen compound catabolic process protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway protein catabolic process endosomal transport modification-dependent protein catabolic process ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway ubiquitin-dependent protein catabolic process organic substance catabolic process secretion establishment of protein localization to extracellular region export from cell protein secretion protein localization to extracellular region secretion by cell cellular catabolic process autophagy microautophagy process utilizing autophagic mechanism ATP transport organic anion transport nucleotide transport purine ribonucleotide transport carbohydrate derivative transport purine nucleotide transport adenine nucleotide transport ATP export nucleobase-containing compound transport organophosphate ester transport	membrane-bounded organelle intracellular anatomical structure endosome intracellular vesicle cellular anatomical entity intracellular membrane-bounded organelle endomembrane system intracellular organelle cytoplasmic vesicle organelle cytoplasm vesicle ESCRT-0 complex protein-containing complex membrane-bounded organelle intracellular anatomical structure endosome intracellular vesicle cellular anatomical entity intracellular membrane-bounded organelle endomembrane system intracellular organelle cytoplasmic vesicle organelle cytoplasm vesicle ESCRT-0 complex protein-containing complex ESCRT complex endosome membrane membrane cytoplasmic vesicle membrane bounding membrane of organelle vesicle membrane organelle membrane vacuolar membrane vacuole
B [auth V]	Vacuolar protein sorting-associated protein 27	protein binding binding protein domain specific binding protein heterodimerization activity protein dimerization activity ubiquitin binding ubiquitin-like protein binding phospholipid binding lipid binding phosphatidylinositol-3-phosphate binding phosphatidylinositol phosphate binding phosphatidylinositol binding cation binding ion binding protein binding binding protein domain specific binding protein heterodimerization activity protein dimerization activity ubiquitin binding ubiquitin-like protein binding phospholipid binding lipid binding phosphatidylinositol-3-phosphate binding phosphatidylinositol phosphate binding phosphatidylinositol binding cation binding ion binding metal ion binding small molecule binding	localization cellular localization late endosome to vacuole transport transport establishment of localization in cell cellular process vacuolar transport intracellular transport establishment of localization vesicle-mediated transport organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process localization cellular localization late endosome to vacuole transport transport establishment of localization in cell cellular process vacuolar transport intracellular transport establishment of localization vesicle-mediated transport organonitrogen compound catabolic process organonitrogen compound metabolic process catabolic process proteolysis involved in protein catabolic process primary metabolic process protein catabolic process macromolecule catabolic process organic substance metabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway ubiquitin-dependent protein catabolic process metabolic process protein metabolic process macromolecule metabolic process organic substance catabolic process multivesicular body sorting pathway regulation of protein maturation positive regulation of protein maturation regulation of cellular process positive regulation of protein metabolic process cellular biosynthetic process positive regulation of biological process positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process regulation of primary metabolic process protein maturation positive regulation of metabolic process positive regulation of biosynthetic process organic substance biosynthetic process positive regulation of macromolecule metabolic process positive regulation of cellular process regulation of biosynthetic process regulation of biological process regulation of protein metabolic process cellular metabolic process biological regulation regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process positive regulation of gene expression regulation of macromolecule biosynthetic process regulation of metabolic process positive regulation of cellular biosynthetic process protein localization establishment of protein localization nitrogen compound transport protein targeting organic substance transport establishment of protein localization to vacuole protein localization to vacuole establishment of protein localization to organelle protein transport cellular macromolecule localization macromolecule localization protein localization to organelle protein targeting to vacuole intracellular protein transport late endosome to vacuole transport via multivesicular body sorting pathway endosome transport via multivesicular body sorting pathway protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway endosomal transport secretion establishment of protein localization to extracellular region export from cell protein secretion protein localization to extracellular region secretion by cell cellular catabolic process autophagy microautophagy process utilizing autophagic mechanism microlipophagy maintenance of protein location protein localization to Golgi apparatus maintenance of location maintenance of location in cell maintenance of protein location in cell protein retention in Golgi apparatus ATP transport organic anion transport nucleotide transport purine ribonucleotide transport carbohydrate derivative transport purine nucleotide transport adenine nucleotide transport ATP export nucleobase-containing compound transport organophosphate ester transport	membrane-bounded organelle ESCRT-0 complex protein-containing complex intracellular anatomical structure ESCRT complex endosome intracellular vesicle cellular anatomical entity intracellular membrane-bounded organelle endomembrane system intracellular organelle cytoplasmic vesicle organelle cytoplasm membrane-bounded organelle ESCRT-0 complex protein-containing complex intracellular anatomical structure ESCRT complex endosome intracellular vesicle cellular anatomical entity intracellular membrane-bounded organelle endomembrane system intracellular organelle cytoplasmic vesicle organelle cytoplasm vesicle endosome membrane membrane cytoplasmic vesicle membrane bounding membrane of organelle vesicle membrane organelle membrane vacuolar membrane vacuole

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth H]	IPR002014	VHS domain	Domain
A [auth H]	IPR008942	ENTH/VHS	Homologous Superfamily
A [auth H]	IPR001452	SH3 domain	Domain
A [auth H]	IPR036028	SH3-like domain superfamily	Homologous Superfamily
A [auth H]	IPR003903	Ubiquitin interacting motif	Conserved Site
B [auth V]	IPR002014	VHS domain	Domain
B [auth V]	IPR008942	ENTH/VHS	Homologous Superfamily
B [auth V]	IPR017455	Zinc finger, FYVE-related	Domain
B [auth V]	IPR000306	FYVE zinc finger	Domain
B [auth V]	IPR011011	Zinc finger, FYVE/PHD-type	Homologous Superfamily
B [auth V]	IPR017073	Hepatocyte growth factor-regulated tyrosine kinase substrate/VPS27	Family
B [auth V]	IPR013083	Zinc finger, RING/FYVE/PHD-type	Homologous Superfamily
B [auth V]	IPR003903	Ubiquitin interacting motif	Conserved Site
B [auth V]	IPR049425	Vacuolar protein sorting-associated protein 27, GAT-like domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.