2PBI

The multifunctional nature of Gbeta5/RGS9 revealed from its crystal structure

External Resource: Annotation


Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

ChainsDomain InfoClassFoldSuperfamilyFamilyDomainSpeciesProvenance Source (Version)
Bd2pbib_ All beta proteins 7-bladed beta-propeller WD40 repeat-like automated matches automated matches house mouse (Mus musculus ) [TaxId: 10090 ], SCOPe (2.08)
Dd2pbid1 All beta proteins 7-bladed beta-propeller WD40 repeat-like automated matches automated matches house mouse (Mus musculus ) [TaxId: 10090 ], SCOPe (2.08)
Dd2pbid2 Artifacts Tags Tags Tags N-terminal Tags house mouse (Mus musculus ) [TaxId: 10090 ], SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

ChainsFamily NameDomain Identifier ArchitecturePossible HomologyHomologyTopologyFamilyProvenance Source (Version)
ADEPe2pbiA4 A: alpha arraysX: HTHH: HTHT: wingedF: DEPECOD (1.6)
ARGSe2pbiA3 A: alpha bundlesX: Regulator of G-protein signaling, RGS (From Topology)H: Regulator of G-protein signaling, RGS (From Topology)T: Regulator of G-protein signaling, RGSF: RGSECOD (1.6)
ARGS_DHEXe2pbiA1 A: alpha bundlesX: Helical extension domain in Regulator of G-protein signaling 9 (From Topology)H: Helical extension domain in Regulator of G-protein signaling 9 (From Topology)T: Helical extension domain in Regulator of G-protein signaling 9F: RGS_DHEXECOD (1.6)
AG-gammae2pbiA2 A: extended segmentsX: Transducin (heterotrimeric G protein), gamma chain (From Topology)H: Transducin (heterotrimeric G protein), gamma chain (From Topology)T: Transducin (heterotrimeric G protein), gamma chainF: G-gammaECOD (1.6)
CDEPe2pbiC5 A: alpha arraysX: HTHH: HTHT: wingedF: DEPECOD (1.6)
CRGSe2pbiC2 A: alpha bundlesX: Regulator of G-protein signaling, RGS (From Topology)H: Regulator of G-protein signaling, RGS (From Topology)T: Regulator of G-protein signaling, RGSF: RGSECOD (1.6)
CRGS_DHEXe2pbiC4 A: alpha bundlesX: Helical extension domain in Regulator of G-protein signaling 9 (From Topology)H: Helical extension domain in Regulator of G-protein signaling 9 (From Topology)T: Helical extension domain in Regulator of G-protein signaling 9F: RGS_DHEXECOD (1.6)
CG-gammae2pbiC3 A: extended segmentsX: Transducin (heterotrimeric G protein), gamma chain (From Topology)H: Transducin (heterotrimeric G protein), gamma chain (From Topology)T: Transducin (heterotrimeric G protein), gamma chainF: G-gammaECOD (1.6)
BANAPC4_WD40_19e2pbiB1 A: beta duplicates or obligate multimersX: beta-propeller-likeH: beta-propellerT: 7-bladedF: ANAPC4_WD40_19ECOD (1.6)
DANAPC4_WD40_18e2pbiD1 A: beta duplicates or obligate multimersX: beta-propeller-likeH: beta-propellerT: 7-bladedF: ANAPC4_WD40_18ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

ChainDomainClassArchitectureTopologyHomologyProvenance Source (Version)
A1.10.10.10 Mainly Alpha Orthogonal Bundle Arc Repressor Mutant, subunit A Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domainCATH (4.3.0)
A1.10.1240.60 Mainly Alpha Orthogonal Bundle Methyltransferase, Methionine Synthase (B12-binding Domains) Chain A, domain 1CATH (4.3.0)
A4.10.260.10 Few Secondary Structures Irregular G Protein Gi Gamma 2 Transducin (heterotrimeric G protein), gamma chainCATH (4.3.0)
A1.10.167.10 Mainly Alpha Orthogonal Bundle Regulator of G-protein Signalling 4 domain 2CATH (4.3.0)
C1.10.10.10 Mainly Alpha Orthogonal Bundle Arc Repressor Mutant, subunit A Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domainCATH (4.3.0)
C1.10.1240.60 Mainly Alpha Orthogonal Bundle Methyltransferase, Methionine Synthase (B12-binding Domains) Chain A, domain 1CATH (4.3.0)
C4.10.260.10 Few Secondary Structures Irregular G Protein Gi Gamma 2 Transducin (heterotrimeric G protein), gamma chainCATH (4.3.0)
C1.10.167.10 Mainly Alpha Orthogonal Bundle Regulator of G-protein Signalling 4 domain 2CATH (4.3.0)
B2.130.10.10 Mainly Beta 7 Propeller Methylamine Dehydrogenase Chain HCATH (4.3.0)
D2.130.10.10 Mainly Beta 7 Propeller Methylamine Dehydrogenase Chain HCATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

ChainsAccessionNameDescriptionCommentsSource
A, C
PF00615Regulator of G protein signaling domain (RGS)Regulator of G protein signaling domainRGS family members are GTPase-activating proteins for heterotrimeric G-protein alpha-subunits.Domain
A, C
PF18148Regulator of G-protein signalling DHEX domain (RGS_DHEX)Regulator of G-protein signalling DHEX domainThis domain is found in RGS9 (Class C) regulator of G-protein signalling (RGS) protein present in Mus musculus. RGS proteins attenuate heterotrimeric G-protein signalling by enhancing the intrinsic GTPase activity of G-alpha subunits and are vital fo ...This domain is found in RGS9 (Class C) regulator of G-protein signalling (RGS) protein present in Mus musculus. RGS proteins attenuate heterotrimeric G-protein signalling by enhancing the intrinsic GTPase activity of G-alpha subunits and are vital for proper signal transduction kinetics. The domain is referred to as DEP helical extension (DHEX) because it is located next to N-terminal Dishevelled/Egl-10/Pleckstrin homology (DEP) domain. Both the DEP and DHEX domains are necessary, but not sufficient, to bind anchoring proteins such as RGS9 anchor protein. DHEX has no close structural homologs [1]. This domain is also found in regulator of G-protein signaling 7 (RGS7), the crystal structure of which has been solved [2].
Domain
A, C
PF00631GGL domain (G-gamma)GGL domainG-protein gamma like domains (GGL) are found in the gamma subunit of the heterotrimeric G protein complex and in regulators of G protein signaling (RGS) proteins [1]. It is also found fused to an inactive Galpha in the Dictyostelium protein gbqA [2] ...G-protein gamma like domains (GGL) are found in the gamma subunit of the heterotrimeric G protein complex and in regulators of G protein signaling (RGS) proteins [1]. It is also found fused to an inactive Galpha in the Dictyostelium protein gbqA [2]. G-gamma likely shares a common origin with the helical N-terminal unit of G-beta [2]. All organisms that posses a G-beta possess a G-gamma [2].
Domain
A, C
PF00610Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP) (DEP)Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)The DEP domain [1] is responsible for mediating intracellular protein targeting and regulation of protein stability in the cell [2-3]. The DEP domain is present in a number of signaling molecules, including Regulator of G protein Signaling (RGS) prot ...The DEP domain [1] is responsible for mediating intracellular protein targeting and regulation of protein stability in the cell [2-3]. The DEP domain is present in a number of signaling molecules, including Regulator of G protein Signaling (RGS) proteins, and has been implicated in membrane targeting [4-5]. New findings in yeast, however, demonstrate a major role for a DEP domain in mediating the interaction of an RGS protein to the C-terminal tail of a GPCR, thus placing RGS in close proximity with its substrate G protein alpha subunit [6-7].
Domain
B, D
PF00400WD domain, G-beta repeat (WD40)WD domain, G-beta repeat- Repeat

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

ChainsPolymerMolecular FunctionBiological ProcessCellular Component
A, C
Regulator of G-protein signaling 9
B, D
Guanine nucleotide-binding protein subunit beta 5

InterPro: Protein Family Classification InterPro Database Homepage

ChainsAccessionNameType
A, C
IPR016137RGS domainDomain
A, C
IPR000591DEP domainDomain
A, C
IPR036305RGS domain superfamilyHomologous Superfamily
A, C
IPR047017Regulator of G-protein signalling 6/7/9/11, DHEX domain superfamilyHomologous Superfamily
A, C
IPR047016Regulator of G protein signaling 6/7/9/11Family
A, C
IPR015898G-protein gamma-like domainDomain
A, C
IPR036388Winged helix-like DNA-binding domain superfamilyHomologous Superfamily
A, C
IPR044926RGS, subdomain 2Homologous Superfamily
A, C
IPR036390Winged helix DNA-binding domain superfamilyHomologous Superfamily
A, C
IPR036284G-protein gamma-like domain superfamilyHomologous Superfamily
A, C
IPR040759Regulator of G-protein signalling, DHEX domainDomain
A, C
IPR047077Regulator of G protein signaling 9, RGS domainDomain
B, D
IPR019775WD40 repeat, conserved siteConserved Site
B, D
IPR015943WD40/YVTN repeat-like-containing domain superfamilyHomologous Superfamily
B, D
IPR016346Guanine nucleotide-binding protein, beta subunitFamily
B, D
IPR036322WD40-repeat-containing domain superfamilyHomologous Superfamily
B, D
IPR001632G-protein, beta subunitDomain
B, D
IPR001680WD40 repeatRepeat
B, D
IPR020472G-protein beta WD-40 repeatRepeat