2P5T

Molecular and structural characterization of the PezAT chromosomal toxin-antitoxin system of the human pathogen Streptococcus pneumoniae

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
H [auth G]	SCOP2B Superfamily	epsilon antitoxin-like	8056334	3001049	SCOP2B (2022-06-29)
B [auth A]	SCOP2 Family	epsilon antitoxin-like	8019195	4002284	SCOP2 (2022-06-29)
B [auth A]	SCOP2 Superfamily	epsilon antitoxin-like	8056334	3001049	SCOP2 (2022-06-29)
D [auth C]	SCOP2B Superfamily	epsilon antitoxin-like	8056334	3001049	SCOP2B (2022-06-29)
F [auth E]	SCOP2B Superfamily	epsilon antitoxin-like	8056334	3001049	SCOP2B (2022-06-29)
E [auth D]	SCOP2B Superfamily	P-loop nucleotide/nucleoside kinase-like	8056333	3002021	SCOP2B (2022-06-29)
I [auth H]	SCOP2B Superfamily	P-loop nucleotide/nucleoside kinase-like	8056333	3002021	SCOP2B (2022-06-29)
C [auth B]	SCOP2 Family	Zeta toxin-like	8056332	4003002	SCOP2 (2022-06-29)
C [auth B]	SCOP2 Superfamily	P-loop nucleotide/nucleoside kinase-like	8056333	3002021	SCOP2 (2022-06-29)
G [auth F]	SCOP2B Superfamily	P-loop nucleotide/nucleoside kinase-like	8056333	3002021	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
H [auth G]	PF08998	e2p5tG1	A: alpha bundles	X: immunoglobulin/albumin-binding domain-like	H: Plasmid maintenance system epsilon/zeta, antidote epsilon subunit (From Topology)	T: Plasmid maintenance system epsilon/zeta, antidote epsilon subunit	F: PF08998	ECOD (1.6)
B [auth A]	PF08998	e2p5tA1	A: alpha bundles	X: immunoglobulin/albumin-binding domain-like	H: Plasmid maintenance system epsilon/zeta, antidote epsilon subunit (From Topology)	T: Plasmid maintenance system epsilon/zeta, antidote epsilon subunit	F: PF08998	ECOD (1.6)
D [auth C]	PF08998	e2p5tC1	A: alpha bundles	X: immunoglobulin/albumin-binding domain-like	H: Plasmid maintenance system epsilon/zeta, antidote epsilon subunit (From Topology)	T: Plasmid maintenance system epsilon/zeta, antidote epsilon subunit	F: PF08998	ECOD (1.6)
F [auth E]	PF08998	e2p5tE1	A: alpha bundles	X: immunoglobulin/albumin-binding domain-like	H: Plasmid maintenance system epsilon/zeta, antidote epsilon subunit (From Topology)	T: Plasmid maintenance system epsilon/zeta, antidote epsilon subunit	F: PF08998	ECOD (1.6)
E [auth D]	PF06414	e2p5tD1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF06414	ECOD (1.6)
I [auth H]	PF06414	e2p5tH1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF06414	ECOD (1.6)
C [auth B]	PF06414	e2p5tB1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF06414	ECOD (1.6)
G [auth F]	PF06414	e2p5tF1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF06414	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
H [auth G]	1.10.8.130	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
B [auth A]	1.10.8.130	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
D [auth C]	1.10.8.130	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
F [auth E]	1.10.8.130	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
E [auth D]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
I [auth H]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
C [auth B]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
G [auth F]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
C [auth B], E [auth D], G [auth F], I [auth H]	PF06414	Zeta toxin (Zeta_toxin)	Zeta toxin	This family consists of several bacterial zeta toxin proteins. Zeta toxin is thought to be part of a postregulational killing system in bacteria. It relies on antitoxin/toxin systems that secure stable inheritance of low and medium copy number plasmi ...	This family consists of several bacterial zeta toxin proteins. Zeta toxin is thought to be part of a postregulational killing system in bacteria. It relies on antitoxin/toxin systems that secure stable inheritance of low and medium copy number plasmids during cell division and kill cells that have lost the plasmid [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth X]	fragment of PezA helix-turn-helix motif	-	-	-
B [auth A], D [auth C], F [auth E], H [auth G]	Putative transcriptional regulator PezA	DNA binding binding organic cyclic compound binding nucleic acid binding	-	-
C [auth B], E [auth D], G [auth F], I [auth H]	PezT	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding kinase activity transferase activity catalytic activity transferase activity, transferring phosphorus-containing groups	phosphorylation cellular metabolic process phosphate-containing compound metabolic process metabolic process cellular process phosphorus metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B [auth A], D [auth C], F [auth E], H [auth G]	IPR035569	Antitoxin epsilon/PezA domain superfamily	Homologous Superfamily
B [auth A], D [auth C], F [auth E], H [auth G]	IPR001387	Cro/C1-type helix-turn-helix domain	Domain
B [auth A], D [auth C], F [auth E], H [auth G]	IPR048181	Antitoxin PezA	Family
B [auth A], D [auth C], F [auth E], H [auth G]	IPR010982	Lambda repressor-like, DNA-binding domain superfamily	Homologous Superfamily
C [auth B], E [auth D], G [auth F], I [auth H]	IPR048180	Toxin PezT	Family
C [auth B], E [auth D], G [auth F], I [auth H]	IPR010488	Zeta toxin domain	Domain
C [auth B], E [auth D], G [auth F], I [auth H]	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.