2OM7

Structural Basis for Interaction of the Ribosome with the Switch Regions of GTP-bound Elongation Factors

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
K [auth E]	d2om7e1	Low resolution protein structures	Ribosome and ribosomal fragments	Ribosome and ribosomal fragments	Ribosome complexes	70S ribosome functional complex	(Thermus thermophilus ) [TaxId: 274 ],	SCOPe (2.08)
L [auth K]	d2om7k1	Multi-domain proteins (alpha and beta)	Ribosomal protein L1	Ribosomal protein L1	Ribosomal protein L1	Ribosomal protein L1	(Thermus thermophilus ) [TaxId: 274 ],	SCOPe (2.08)
N	d2om7n1	Alpha and beta proteins (a/b)	Flavodoxin-like	Ribosomal protein S2	Ribosomal protein S2	Ribosomal protein S2	(Thermus thermophilus ) [TaxId: 274 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
K [auth E]	SCOP2B Superfamily	Nucleic acid-binding proteins	8044021	3000135	SCOP2B (2022-06-29)
N	SCOP2B Superfamily	SIS domain/Ribosomal protein S2-like	8044010	3001397	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
K [auth E]	PF00164	e2om7E1	A: beta barrels	X: OB-fold	H: Nucleic acid-binding proteins (From Topology)	T: Nucleic acid-binding proteins	F: PF00164	ECOD (1.6)
L [auth K]	PF00687	e2om7K6	A: a+b two layers	X: a+b domain in Ribosomal protein L1 (From Topology)	H: a+b domain in Ribosomal protein L1 (From Topology)	T: a+b domain in Ribosomal protein L1	F: PF00687	ECOD (1.6)
L [auth K]	PF00687	e2om7K5	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Rossmann-like domain in Ribosomal protein L1 (From Topology)	T: Rossmann-like domain in Ribosomal protein L1	F: PF00687	ECOD (1.6)
M [auth L]	PF03144	e2om7L21	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PF03144	ECOD (1.6)
M [auth L]	PF03764	e2om7L24	A: a+b two layers	X: Ribosomal protein S5 domain 2-like (From Topology)	H: Ribosomal protein S5 domain 2-like (From Topology)	T: Ribosomal protein S5 domain 2-like	F: PF03764	ECOD (1.6)
M [auth L]	PF00679	e2om7L20	A: a+b two layers	X: Alpha-beta plaits	H: EF-G C-terminal domain-like (From Topology)	T: EF-G C-terminal domain-like	F: PF00679	ECOD (1.6)
M [auth L]	PF14492	e2om7L22	A: a+b two layers	X: Alpha-beta plaits	H: EF-G C-terminal domain-like (From Topology)	T: EF-G C-terminal domain-like	F: PF14492	ECOD (1.6)
M [auth L]	PF00009	e2om7L23	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00009	ECOD (1.6)
N	PF00318	e2om7N1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Ribosomal protein S2/SIS domain (From Topology)	T: Ribosomal protein S2/SIS domain	F: PF00318	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
K [auth E]	PF00164	Ribosomal protein S12/S23 (Ribosom_S12_S23)	Ribosomal protein S12/S23	-	Family
M [auth L]	PF00679	Elongation factor G C-terminus (EFG_C)	Elongation factor G C-terminus	This domain includes the carboxyl terminal regions of Elongation factor G, elongation factor 2 and some tetracycline resistance proteins and adopt a ferredoxin-like fold.	Domain
M [auth L]	PF00009	Elongation factor Tu GTP binding domain (GTP_EFTU)	Elongation factor Tu GTP binding domain	This domain contains a P-loop motif, also found in several other families such as Pfam:PF00071, Pfam:PF00025 and Pfam:PF00063. Elongation factor Tu consists of three structural domains, this plus two C-terminal beta barrel domains.	Domain
M [auth L]	PF14492	Elongation Factor G, domain III (EFG_III)	Elongation Factor G, domain III	This domain is found in Elongation Factor G. It shares a similar structure with domain V (Pfam:PF00679). Structural studies in drosophila indicate this is domain 3 [1].	Domain
M [auth L]	PF03144	Elongation factor Tu domain 2 (GTP_EFTU_D2)	Elongation factor Tu domain 2	Elongation factor Tu consists of three structural domains, this is the second domain. This domain adopts a beta barrel structure. This the second domain is involved in binding to charged tRNA [1]. This domain is also found in other proteins such as e ...	Elongation factor Tu consists of three structural domains, this is the second domain. This domain adopts a beta barrel structure. This the second domain is involved in binding to charged tRNA [1]. This domain is also found in other proteins such as elongation factor G and translation initiation factor IF-2. This domain is structurally related to Pfam:PF03143, and in fact has weak sequence matches to this domain.	Domain
M [auth L]	PF03764	Elongation factor G, domain IV (EFG_IV)	Elongation factor G, domain IV	This domain is found in elongation factor G, elongation factor 2 and some tetracycline resistance proteins and adopts a ribosomal protein S5 domain 2-like fold.	Domain
N	PF00318	Ribosomal protein S2 (Ribosomal_S2)	Ribosomal protein S2	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
I [auth J]	Fragment of23S rRNA (H76)	-	-	-
K [auth E]	30S ribosomal protein S12	RNA binding binding organic cyclic compound binding tRNA binding nucleic acid binding structural molecule activity structural constituent of ribosome rRNA binding	organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process organonitrogen compound biosynthetic process primary metabolic process translation gene expression macromolecule biosynthetic process organic substance metabolic process biosynthetic process organic substance biosynthetic process metabolic process cellular process protein metabolic process organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process organonitrogen compound biosynthetic process primary metabolic process translation gene expression macromolecule biosynthetic process organic substance metabolic process biosynthetic process organic substance biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process	protein-containing complex intracellular anatomical structure small ribosomal subunit cellular anatomical entity ribosomal subunit intracellular organelle organelle ribonucleoprotein complex intracellular non-membrane-bounded organelle ribosome non-membrane-bounded organelle
A	Fragment of 16S rRNA (h14)	-	-	-
J [auth M]	p/E-tRNA	-	-	-
L [auth K]	50S ribosomal protein L1	RNA binding binding organic cyclic compound binding tRNA binding nucleic acid binding structural molecule activity structural constituent of ribosome rRNA binding	regulation of cellular process cellular biosynthetic process organonitrogen compound biosynthetic process regulation of primary metabolic process post-transcriptional regulation of gene expression organic substance metabolic process organic substance biosynthetic process protein metabolic process regulation of biosynthetic process regulation of biological process organonitrogen compound metabolic process cellular metabolic process regulation of protein metabolic process biological regulation regulation of cellular process cellular biosynthetic process organonitrogen compound biosynthetic process regulation of primary metabolic process post-transcriptional regulation of gene expression organic substance metabolic process organic substance biosynthetic process protein metabolic process regulation of biosynthetic process regulation of biological process organonitrogen compound metabolic process cellular metabolic process regulation of protein metabolic process biological regulation regulation of cellular metabolic process primary metabolic process regulation of macromolecule metabolic process gene expression translation macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process regulation of translation regulation of macromolecule biosynthetic process metabolic process cellular process macromolecule metabolic process regulation of metabolic process	protein-containing complex intracellular anatomical structure cellular anatomical entity ribosomal subunit intracellular organelle organelle ribonucleoprotein complex intracellular non-membrane-bounded organelle ribosome large ribosomal subunit non-membrane-bounded organelle
M [auth L]	Elongation factor G	translation regulator activity binding organic cyclic compound binding translation regulator activity, nucleic acid binding translation elongation factor activity nucleic acid binding translation factor activity, RNA binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding translation regulator activity binding organic cyclic compound binding translation regulator activity, nucleic acid binding translation elongation factor activity nucleic acid binding translation factor activity, RNA binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	-	intracellular anatomical structure cytoplasm cellular anatomical entity
N	30S ribosomal protein S2	structural molecule activity structural constituent of ribosome RNA binding binding organic cyclic compound binding rRNA binding nucleic acid binding	organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process organonitrogen compound biosynthetic process primary metabolic process translation gene expression macromolecule biosynthetic process organic substance metabolic process biosynthetic process organic substance biosynthetic process metabolic process cellular process protein metabolic process organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process organonitrogen compound biosynthetic process primary metabolic process translation gene expression macromolecule biosynthetic process organic substance metabolic process biosynthetic process organic substance biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process	protein-containing complex intracellular anatomical structure small ribosomal subunit cellular anatomical entity ribosomal subunit intracellular organelle organelle ribonucleoprotein complex intracellular non-membrane-bounded organelle ribosome non-membrane-bounded organelle
B	Fragment of 16S rRNA (h15)	-	-	-
C	Fragment of 16S rRNA (h44)	-	-	-
D	16S ribosomal RNA (H5)	-	-	-
E [auth F]	Fragment of23S rRNA (H95)	-	-	-
F [auth G]	Fragment of23S rRNA (H68)	-	-	-
G [auth H]	Fragment of23S rRNA (H89)	-	-	-
H [auth I]	Fragment of23S rRNA (H42-44)	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
K [auth E]	IPR006032	Small ribosomal subunit protein uS12	Family
K [auth E]	IPR012340	Nucleic acid-binding, OB-fold	Homologous Superfamily
K [auth E]	IPR005679	Ribosomal protein uS12, bacterial-type	Family
L [auth K]	IPR023673	Large ribosomal subunit protein uL1, conserved site	Conserved Site
L [auth K]	IPR016095	Large ribosomal subunit protein uL1, 3-layer alpha/beta-sandwich domain	Homologous Superfamily
L [auth K]	IPR005878	Large ribosomal subunit protein uL1, bacterial-type	Family
L [auth K]	IPR023674	Ribosomal protein uL1-like	Homologous Superfamily
L [auth K]	IPR002143	Large ribosomal subunit protein uL1	Family
L [auth K]	IPR028364	Ribosomal protein uL1/ribosomal biogenesis protein	Family
M [auth L]	IPR005517	Translation elongation factor EFG/EF2, domain IV	Domain
M [auth L]	IPR004540	Translation elongation factor EFG/EF2	Family
M [auth L]	IPR000795	Translational (tr)-type GTP-binding domain	Domain
M [auth L]	IPR000640	Elongation factor EFG, domain V-like	Domain
M [auth L]	IPR031157	Tr-type G domain, conserved site	Conserved Site
M [auth L]	IPR009022	Elongation factor G, domain III	Domain
M [auth L]	IPR035647	EF-G domain III/V-like	Homologous Superfamily
M [auth L]	IPR041095	Elongation Factor G, domain II	Domain
M [auth L]	IPR004161	Translation elongation factor EFTu-like, domain 2	Domain
M [auth L]	IPR047872	Elongation factor G, domain IV	Domain
M [auth L]	IPR009000	Translation protein, beta-barrel domain superfamily	Homologous Superfamily
M [auth L]	IPR020568	Ribosomal protein uS5 domain 2-type superfamily	Homologous Superfamily
M [auth L]	IPR005225	Small GTP-binding protein domain	Domain
M [auth L]	IPR035649	EFG, domain V	Domain
M [auth L]	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
M [auth L]	IPR014721	Small ribosomal subunit protein uS5 domain 2-type fold, subgroup	Homologous Superfamily
N	IPR005706	Small ribosomal subunit protein uS2, bacteria/mitochondria/plastid	Family
N	IPR023591	Small ribosomal subunit protein uS2, flavodoxin-like domain superfamily	Homologous Superfamily
N	IPR018130	Small ribosomal subunit protein uS2, conserved site	Conserved Site
N	IPR001865	Small ribosomal subunit protein uS2	Family