2LAS

Molecular Determinants of Paralogue-Specific SUMO-SIM Recognition

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ubiquitin-like	8043414	3000157	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Rad60-SLD	e2lasA1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: Rad60-SLD	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.10.20.90	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Phosphatidylinositol 3-kinase Catalytic Subunit	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF11976	Ubiquitin-2 like Rad60 SUMO-like (Rad60-SLD)	Ubiquitin-2 like Rad60 SUMO-like	-	Family
B	PF12185	Nup358/RanBP2 E3 ligase domain (IR1-M)	Nup358/RanBP2 E3 ligase domain	This domain family is found in eukaryotes, and is approximately 60 amino acids in length. The family is found in association with Pfam:PF00638, Pfam:PF00641, Pfam:PF00160. There are two conserved sequence motifs: TFFC and EDF. Nup358/RanBP2 is a nucl ...	This domain family is found in eukaryotes, and is approximately 60 amino acids in length. The family is found in association with Pfam:PF00638, Pfam:PF00641, Pfam:PF00160. There are two conserved sequence motifs: TFFC and EDF. Nup358/RanBP2 is a nucleoporin involved in ubiquitination of many different protein targets from various cellular pathways. It complexes with Ubc9, SUMO-1 and RanGAP1 to perform this function. This is the ligase domain which binds to Ubc9.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Small ubiquitin-related modifier 1	protein binding binding small protein activating enzyme binding enzyme binding RNA binding organic cyclic compound binding nucleic acid binding ubiquitin-specific protease binding protease binding transcription factor binding ubiquitin-like protein ligase binding protein tag activity molecular tag activity potassium channel regulator activity protein binding binding small protein activating enzyme binding enzyme binding RNA binding organic cyclic compound binding nucleic acid binding ubiquitin-specific protease binding protease binding transcription factor binding ubiquitin-like protein ligase binding protein tag activity molecular tag activity potassium channel regulator activity channel regulator activity ion channel regulator activity molecular function regulator activity transporter regulator activity ubiquitin protein ligase binding	regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process negative regulation of metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process organic substance metabolic process negative regulation of cellular process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process biological regulation regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process cellular process protein localization negative regulation of protein transport regulation of localization establishment of protein localization negative regulation of nucleocytoplasmic transport nitrogen compound transport nucleocytoplasmic transport nuclear transport negative regulation of intracellular protein transport negative regulation of transport localization import into nucleus cellular macromolecule localization establishment of localization in cell regulation of intracellular transport organic substance transport negative regulation of establishment of protein localization intracellular transport regulation of nucleocytoplasmic transport protein localization to nucleus establishment of localization establishment of protein localization to organelle regulation of intracellular protein transport intracellular protein transport cellular localization regulation of protein localization transport regulation of protein localization to nucleus negative regulation of protein import into nucleus regulation of protein import into nucleus protein import into nucleus negative regulation of protein localization negative regulation of intracellular transport regulation of transport regulation of establishment of protein localization regulation of protein transport negative regulation of protein localization to nucleus protein transport regulation of cellular localization macromolecule localization protein localization to organelle regulation of protein stability regulation of biological quality protein stabilization negative regulation of DNA-binding transcription factor activity negative regulation of molecular function regulation of molecular function regulation of DNA-binding transcription factor activity positive regulation of cellular component organization regulation of cellular component organization protein-containing complex organization positive regulation of cellular component biogenesis positive regulation of biological process regulation of cellular component biogenesis cellular component organization cellular component biogenesis positive regulation of protein-containing complex assembly regulation of protein-containing complex assembly cellular component assembly protein-containing complex assembly cellular component organization or biogenesis positive regulation of cellular process cellular response to stimulus cellular response to heat response to stimulus cellular response to stress response to stress response to heat response to abiotic stimulus response to temperature stimulus organonitrogen compound metabolic process peptidyl-lysine modification protein sumoylation macromolecule modification peptidyl-amino acid modification protein metabolic process protein modification process post-translational protein modification protein modification by small protein conjugation protein modification by small protein conjugation or removal positive regulation of proteasomal protein catabolic process catabolic process positive regulation of protein metabolic process proteolysis involved in protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process macromolecule catabolic process regulation of ubiquitin-dependent protein catabolic process positive regulation of metabolic process positive regulation of ubiquitin-dependent protein catabolic process regulation of proteolysis regulation of proteolysis involved in protein catabolic process modification-dependent macromolecule catabolic process proteolysis positive regulation of macromolecule metabolic process organonitrogen compound catabolic process regulation of protein metabolic process proteasomal protein catabolic process protein catabolic process regulation of catabolic process positive regulation of protein catabolic process positive regulation of proteolysis involved in protein catabolic process positive regulation of catabolic process regulation of proteasomal ubiquitin-dependent protein catabolic process modification-dependent protein catabolic process positive regulation of proteolysis regulation of proteasomal protein catabolic process positive regulation of proteasomal ubiquitin-dependent protein catabolic process ubiquitin-dependent protein catabolic process organic substance catabolic process regulation of protein catabolic process negative regulation of ion transmembrane transporter activity negative regulation of potassium ion transmembrane transporter activity regulation of delayed rectifier potassium channel activity negative regulation of voltage-gated potassium channel activity negative regulation of cation transmembrane transport monoatomic cation transport regulation of potassium ion transmembrane transporter activity regulation of monoatomic ion transport inorganic ion transmembrane transport regulation of potassium ion transmembrane transport regulation of cation channel activity inorganic cation transmembrane transport transmembrane transport regulation of transmembrane transport potassium ion transport regulation of potassium ion transport negative regulation of cation channel activity monoatomic ion transmembrane transport negative regulation of monoatomic ion transport regulation of transmembrane transporter activity potassium ion transmembrane transport regulation of monoatomic ion transmembrane transport negative regulation of transmembrane transport metal ion transport regulation of transporter activity monoatomic cation transmembrane transport negative regulation of monoatomic ion transmembrane transport negative regulation of delayed rectifier potassium channel activity monoatomic ion transport regulation of metal ion transport negative regulation of potassium ion transport regulation of monoatomic ion transmembrane transporter activity negative regulation of transporter activity negative regulation of potassium ion transmembrane transport regulation of monoatomic cation transmembrane transport regulation of action potential negative regulation of action potential action potential regulation of membrane potential DNA metabolic process DNA damage response DNA repair regulation of DNA binding regulation of binding negative regulation of binding negative regulation of DNA binding cellular response to inorganic substance cellular response to metal ion response to cadmium ion response to inorganic substance cellular response to chemical stimulus response to metal ion response to chemical cellular response to cadmium ion negative regulation of transcription by transcription factor localization developmental process anatomical structure development roof of mouth development	membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle cellular anatomical entity nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle intracellular non-membrane-bounded organelle nuclear lumen non-membrane-bounded organelle membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle cellular anatomical entity nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle intracellular non-membrane-bounded organelle nuclear lumen non-membrane-bounded organelle nuclear speck nuclear body nucleoplasm nuclear membrane membrane endomembrane system organelle envelope nuclear envelope organelle membrane cytoplasm cytosol cell periphery plasma membrane protein-containing complex nuclear protein-containing complex nuclear pore nuclear stress granule ribonucleoprotein granule supramolecular complex PML body
B	M-IR2_peptide	small GTPase binding protein binding binding enzyme binding GTPase binding RNA binding organic cyclic compound binding nucleic acid binding aminoacyltransferase activity ubiquitin-like protein transferase activity catalytic activity, acting on a protein acyltransferase activity SUMO transferase activity transferase activity small GTPase binding protein binding binding enzyme binding GTPase binding RNA binding organic cyclic compound binding nucleic acid binding aminoacyltransferase activity ubiquitin-like protein transferase activity catalytic activity, acting on a protein acyltransferase activity SUMO transferase activity transferase activity catalytic activity SUMO ligase activity ubiquitin-like protein ligase activity protein-containing complex binding cation binding ion binding metal ion binding small molecule binding	monosaccharide metabolic process gluconeogenesis regulation of primary metabolic process organic substance metabolic process regulation of small molecule metabolic process organic substance biosynthetic process small molecule metabolic process regulation of carbohydrate metabolic process regulation of glucose metabolic process regulation of biosynthetic process carbohydrate metabolic process regulation of biological process small molecule biosynthetic process carbohydrate biosynthetic process monosaccharide metabolic process gluconeogenesis regulation of primary metabolic process organic substance metabolic process regulation of small molecule metabolic process organic substance biosynthetic process small molecule metabolic process regulation of carbohydrate metabolic process regulation of glucose metabolic process regulation of biosynthetic process carbohydrate metabolic process regulation of biological process small molecule biosynthetic process carbohydrate biosynthetic process biological regulation hexose metabolic process primary metabolic process monosaccharide biosynthetic process hexose biosynthetic process biosynthetic process regulation of gluconeogenesis metabolic process regulation of carbohydrate biosynthetic process regulation of metabolic process glucose metabolic process localization centrosome localization microtubule organizing center localization cellular process RNA transport nitrogen compound transport transport organic substance transport nucleic acid transport mRNA transport establishment of localization establishment of RNA localization macromolecule localization RNA localization nucleobase-containing compound transport organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process gene expression macromolecule biosynthetic process protein maturation protein folding protein metabolic process macromolecule metabolic process cellular localization nucleocytoplasmic transport nuclear transport establishment of localization in cell intracellular transport peptidyl-lysine modification protein sumoylation macromolecule modification peptidyl-amino acid modification protein modification process post-translational protein modification protein modification by small protein conjugation protein modification by small protein conjugation or removal nuclear export protein localization establishment of protein localization NLS-bearing protein import into nucleus import into nucleus cellular macromolecule localization protein import into nucleus protein localization to nucleus establishment of protein localization to organelle protein transport protein localization to organelle intracellular protein transport response to amine response to organic substance response to stimulus response to organonitrogen compound response to chemical response to nitrogen compound response to amphetamine	membrane cellular anatomical entity membrane-bounded organelle intracellular organelle organelle envelope intracellular anatomical structure organelle nuclear envelope nucleus intracellular membrane-bounded organelle endomembrane system nuclear membrane organelle membrane cytoplasmic periphery of the nuclear pore complex membrane cellular anatomical entity membrane-bounded organelle intracellular organelle organelle envelope intracellular anatomical structure organelle nuclear envelope nucleus intracellular membrane-bounded organelle endomembrane system nuclear membrane organelle membrane cytoplasmic periphery of the nuclear pore complex perinuclear region of cytoplasm cytoplasm cytosol protein-containing complex nuclear protein-containing complex nuclear pore membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen nuclear pore nuclear basket catalytic complex SUMO ligase complex transferase complex inclusion body nuclear inclusion body nuclear outer membrane-endoplasmic reticulum membrane network annulate lamellae nuclear pore cytoplasmic filaments

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR046332	Small ubiquitin-related modifier 1, Ubl domain	Domain
A	IPR000626	Ubiquitin-like domain	Domain
A	IPR029071	Ubiquitin-like domain superfamily	Homologous Superfamily
A	IPR022617	Rad60/SUMO-like domain	Domain
B	IPR022011	Nup358/RanBP2 E3 ligase domain	Domain
B	IPR029000	Cyclophilin-like domain superfamily	Homologous Superfamily
B	IPR002130	Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain	Domain
B	IPR011990	Tetratricopeptide-like helical domain superfamily	Homologous Superfamily
B	IPR011993	PH-like domain superfamily	Homologous Superfamily
B	IPR019734	Tetratricopeptide repeat	Repeat
B	IPR020892	Cyclophilin-type peptidyl-prolyl cis-trans isomerase, conserved site	Conserved Site
B	IPR036443	Zinc finger, RanBP2-type superfamily	Homologous Superfamily
B	IPR000156	Ran binding domain	Domain
B	IPR001876	Zinc finger, RanBP2-type	Domain
B	IPR045255	Ran binding protein RanBP1-like	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P63165	orofacial cleft 10 cleft palate Neoplasm Invasiveness cleft lip pancreatic intraductal papillary-mucinous neoplasm
B	Pharos: P49792	familial acute necrotizing encephalopathy inflammatory myofibroblastic tumor psoriasis acute necrotizing encephalitis breast carcinoma ectodermal dysplasia 10B, hypohidrotic/hair/tooth type, autosomal recessive hereditary spastic paraplegia lung cancer ovarian cancer tuberculosis adrenal cortex carcinoma dermatomyositis ENCEPHALOPATHY, ACUTE, INFECTION-INDUCED, SUSCEPTIBILITY TO, 3 glioblastoma hypohidrotic ectodermal dysplasia familial acute necrotizing encephalopathy inflammatory myofibroblastic tumor psoriasis acute necrotizing encephalitis breast carcinoma ectodermal dysplasia 10B, hypohidrotic/hair/tooth type, autosomal recessive hereditary spastic paraplegia lung cancer ovarian cancer tuberculosis adrenal cortex carcinoma dermatomyositis ENCEPHALOPATHY, ACUTE, INFECTION-INDUCED, SUSCEPTIBILITY TO, 3 glioblastoma hypohidrotic ectodermal dysplasia malignant mesothelioma plasma cell myeloma atypical teratoid rhabdoid tumor ectodermal dysplasia 10A, hypohidrotic/hair/nail type, autosomal dominant myopathy

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.