Annotations: 2K2F

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C [auth A]	d2k2fa_	All alpha proteins	EF Hand-like	EF-hand	S100 proteins	Calcyclin (S100)	rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)
D [auth B]	d2k2fb_	All alpha proteins	EF Hand-like	EF-hand	S100 proteins	Calcyclin (S100)	rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C [auth A]	SCOP2B Superfamily	EF-hand	8035573	3001983	SCOP2B (2022-06-29)
D [auth B]	SCOP2B Superfamily	EF-hand	8035573	3001983	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C [auth A]	PF00036,PF01023	e2k2fA1	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: PF00036,PF01023	ECOD (1.6)
D [auth B]	PF00036,PF01023	e2k2fB1	A: alpha arrays	X: EF-hand	H: EF-hand-related	T: EF-hand	F: PF00036,PF01023	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C [auth A]	1.10.238.10	Mainly Alpha	Orthogonal Bundle	Recoverin	domain 1	CATH (4.3.0)
D [auth B]	1.10.238.10	Mainly Alpha	Orthogonal Bundle	Recoverin	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
C [auth A], D [auth B]	PF01023	S-100/ICaBP type calcium binding domain (S_100)	S-100/ICaBP type calcium binding domain	The S-100 domain is a subfamily of the EF-hand calcium binding proteins.	Domain
C [auth A], D [auth B]	PF00036	EF hand (EF-hand_1)	EF hand	The EF-hands can be divided into two classes: signalling proteins and buffering/transport proteins. The first group is the largest and includes the most well-known members of the family such as calmodulin, troponin C and S100B. These proteins typica ...	The EF-hands can be divided into two classes: signalling proteins and buffering/transport proteins. The first group is the largest and includes the most well-known members of the family such as calmodulin, troponin C and S100B. These proteins typically undergo a calcium-dependent conformational change which opens a target binding site. The latter group is represented by calbindin D9k and do not undergo calcium dependent conformational changes.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth C], B [auth D]	Ryanodine receptor 1 peptide	calcium-induced calcium release activity calcium channel activity monoatomic cation transmembrane transporter activity intracellularly ligand-gated monoatomic ion channel activity gated channel activity monoatomic ion channel activity ryanodine-sensitive calcium-release channel activity intracellularly gated calcium channel activity ligand-gated channel activity ligand-gated monoatomic ion channel activity monoatomic cation channel activity passive transmembrane transporter activity calcium ion transmembrane transporter activity inorganic molecular entity transmembrane transporter activity channel activity calcium-induced calcium release activity calcium channel activity monoatomic cation transmembrane transporter activity intracellularly ligand-gated monoatomic ion channel activity gated channel activity monoatomic ion channel activity ryanodine-sensitive calcium-release channel activity intracellularly gated calcium channel activity ligand-gated channel activity ligand-gated monoatomic ion channel activity monoatomic cation channel activity passive transmembrane transporter activity calcium ion transmembrane transporter activity inorganic molecular entity transmembrane transporter activity channel activity ligand-gated calcium channel activity inorganic cation transmembrane transporter activity monoatomic ion transmembrane transporter activity transporter activity metal ion transmembrane transporter activity ligand-gated monoatomic cation channel activity transmembrane transporter activity amide binding binding organic acid binding ion binding organic cyclic compound binding anion binding suramin binding sulfur compound binding small molecule binding protein binding calmodulin binding identical protein binding cation binding calcium ion binding metal ion binding scaffold protein binding kinase binding enzyme binding protein kinase binding protein kinase A binding protein kinase A regulatory subunit binding protein kinase A catalytic subunit binding	response to stimulus response to activity response to muscle activity response to chemical response to xenobiotic stimulus heart process multicellular organismal process system process regulation of biological process regulation of heart contraction regulation of system process biological regulation positive regulation of biological process positive regulation of heart rate response to stimulus response to activity response to muscle activity response to chemical response to xenobiotic stimulus heart process multicellular organismal process system process regulation of biological process regulation of heart contraction regulation of system process biological regulation positive regulation of biological process positive regulation of heart rate positive regulation of blood circulation positive regulation of heart contraction circulatory system process regulation of blood circulation positive regulation of multicellular organismal process regulation of multicellular organismal process regulation of biological quality heart contraction blood circulation regulation of heart rate muscle contraction muscle system process striated muscle contraction positive regulation of the force of heart contraction regulation of the force of heart contraction response to metal ion response to calcium ion response to inorganic substance response to redox state regulation of AV node cell action potential AV node cell action potential regulation of cellular process signaling cell-cell signaling involved in cardiac conduction cell communication regulation of cardiac muscle cell action potential cell communication involved in cardiac conduction regulation of action potential AV node cell to bundle of His cell signaling cell-cell signaling action potential AV node cell to bundle of His cell communication cardiac conduction regulation of cell communication cellular process cardiac muscle cell action potential regulation of membrane potential regulation of actin filament-based process cardiac muscle cell action potential involved in contraction regulation of ventricular cardiac muscle cell action potential cardiac muscle contraction actin filament-based process cardiac muscle cell contraction ventricular cardiac muscle cell action potential regulation of cardiac muscle contraction actin filament-based movement regulation of muscle system process regulation of striated muscle contraction regulation of actin filament-based movement regulation of muscle contraction regulation of cardiac muscle cell contraction actin-mediated cell contraction response to organic substance response to organonitrogen compound response to nitrogen compound response to purine-containing compound response to organic cyclic compound response to caffeine response to alkaloid cellular response to stimulus cellular response to nitrogen compound response to endogenous stimulus cellular response to caffeine cellular response to organic cyclic compound cellular response to endogenous stimulus cellular response to chemical stimulus cellular response to organonitrogen compound cellular response to organic substance cellular response to alkaloid cellular response to purine-containing compound inorganic ion homeostasis intracellular calcium ion homeostasis homeostatic process calcium ion homeostasis cellular homeostasis chemical homeostasis intracellular monoatomic cation homeostasis monoatomic ion homeostasis intracellular chemical homeostasis monoatomic cation homeostasis intracellular monoatomic ion homeostasis regulation of cytosolic calcium ion concentration SA node cell to atrial cardiac muscle cell signaling SA node cell to atrial cardiac muscle cell communication SA node cell action potential regulation of SA node cell action potential transport monoatomic ion transmembrane transport inorganic ion transmembrane transport establishment of localization calcium ion transport metal ion transport inorganic cation transmembrane transport transmembrane transport monoatomic cation transmembrane transport localization monoatomic ion transport monoatomic cation transport calcium ion transmembrane transport regulation of localization cellular localization maintenance of location in cell positive regulation of sequestering of calcium ion regulation of sequestering of calcium ion positive regulation of cellular process sequestering of calcium ion maintenance of location heart morphogenesis embryo development embryonic heart tube development multicellular organism development tube morphogenesis embryonic heart tube morphogenesis anatomical structure development animal organ morphogenesis epithelial tube morphogenesis embryonic organ development system development embryonic organ morphogenesis developmental process heart development epithelium development tube development embryonic morphogenesis animal organ development morphogenesis of an epithelium tissue development circulatory system development anatomical structure morphogenesis tissue morphogenesis negative regulation of cytosolic calcium ion concentration regulation of calcium ion transport negative regulation of biological process sarcoplasmic reticulum calcium ion transport release of sequestered calcium ion into cytosol by sarcoplasmic reticulum intracellular signal transduction intracellular signaling cassette signal transduction release of sequestered calcium ion into cytosol by endoplasmic reticulum regulation of release of sequestered calcium ion into cytosol regulation of monoatomic ion transport release of sequestered calcium ion into cytosol regulation of transmembrane transport calcium-mediated signaling regulation of monoatomic ion transmembrane transport negative regulation of sequestering of calcium ion regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion regulation of calcium ion transmembrane transport negative regulation of cellular process regulation of metal ion transport calcium ion transmembrane import into cytosol regulation of cardiac muscle contraction by calcium ion signaling regulation of transport regulation of monoatomic cation transmembrane transport regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum response to decreased oxygen levels response to hypoxia response to stress response to oxygen levels response to abiotic stimulus regulation of atrial cardiac muscle cell action potential regulation of cardiac conduction atrial cardiac muscle cell to AV node cell signaling atrial cardiac muscle cell to AV node cell communication atrial cardiac muscle cell action potential manganese ion transport transition metal ion transport manganese ion transmembrane transport positive regulation of cytosolic calcium ion concentration calcium ion transport into cytosol apoptotic process cell death programmed cell death epithelial cell apoptotic process type B pancreatic cell apoptotic process protein localization establishment of protein localization establishment of protein localization to organelle cellular macromolecule localization macromolecule localization protein localization to organelle establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum ventricular cardiac muscle tissue morphogenesis striated muscle tissue development muscle structure development left ventricular cardiac muscle tissue morphogenesis ventricular cardiac muscle tissue development cardiac chamber development muscle tissue development muscle organ development cardiac chamber morphogenesis cardiac muscle tissue development muscle tissue morphogenesis cardiac ventricle morphogenesis cardiac muscle tissue morphogenesis cardiac left ventricle morphogenesis cardiac ventricle development muscle organ morphogenesis establishment of localization in cell response to epinephrine cellular response to epinephrine stimulus muscle hypertrophy cardiac muscle hypertrophy striated muscle hypertrophy Purkinje myocyte to ventricular cardiac muscle cell communication Purkinje myocyte to ventricular cardiac muscle cell signaling response to nutrient levels response to nutrient detection of stimulus detection of chemical stimulus detection of calcium ion response to mechanical stimulus response to external stimulus response to muscle stretch response to magnesium ion	membrane cellular anatomical entity intracellular anatomical structure Z disc sarcomere intracellular organelle I band organelle intracellular non-membrane-bounded organelle contractile muscle fiber cytoplasm supramolecular fiber myofibril supramolecular polymer membrane cellular anatomical entity intracellular anatomical structure Z disc sarcomere intracellular organelle I band organelle intracellular non-membrane-bounded organelle contractile muscle fiber cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex non-membrane-bounded organelle membrane-bounded organelle organelle envelope nuclear envelope nucleus intracellular membrane-bounded organelle endomembrane system cell periphery sarcolemma plasma membrane endoplasmic reticulum smooth endoplasmic reticulum protein-containing complex transmembrane transporter complex membrane protein complex cation channel complex transporter complex calcium channel complex monoatomic ion channel complex extrinsic component of plasma membrane cytoplasmic side of plasma membrane cytoplasmic side of membrane extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane side of membrane sarcoplasm sarcoplasmic reticulum A band organelle subcompartment endoplasmic reticulum subcompartment bounding membrane of organelle nuclear outer membrane-endoplasmic reticulum membrane network sarcoplasmic reticulum membrane organelle membrane endoplasmic reticulum membrane
C [auth A], D [auth B]	Protein S100-A1	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding protein binding S100 protein binding identical protein binding protein homodimerization activity protein dimerization activity calcium-dependent protein binding ATPase binding enzyme binding	heart process multicellular organismal process system process regulation of biological process regulation of heart contraction regulation of system process biological regulation circulatory system process regulation of blood circulation regulation of multicellular organismal process heart contraction blood circulation regulation of sprouting angiogenesis regulation of developmental process heart process multicellular organismal process system process regulation of biological process regulation of heart contraction regulation of system process biological regulation circulatory system process regulation of blood circulation regulation of multicellular organismal process heart contraction blood circulation regulation of sprouting angiogenesis regulation of developmental process positive regulation of biological process angiogenesis regulation of vasculature development positive regulation of developmental process multicellular organism development tube morphogenesis anatomical structure development positive regulation of sprouting angiogenesis regulation of anatomical structure morphogenesis blood vessel development system development developmental process regulation of multicellular organismal development blood vessel morphogenesis tube development positive regulation of angiogenesis sprouting angiogenesis vasculature development circulatory system development anatomical structure morphogenesis positive regulation of vasculature development regulation of angiogenesis anatomical structure formation involved in morphogenesis positive regulation of multicellular organismal process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II negative regulation of DNA-templated transcription negative regulation of transcription by RNA polymerase II cellular metabolic process negative regulation of metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process organic substance metabolic process negative regulation of cellular process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process cellular process	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle M band sarcomere intracellular non-membrane-bounded organelle contractile muscle fiber A band supramolecular fiber membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle M band sarcomere intracellular non-membrane-bounded organelle contractile muscle fiber A band supramolecular fiber myofibril supramolecular polymer supramolecular complex non-membrane-bounded organelle Z disc I band endomembrane system sarcoplasm endoplasmic reticulum sarcoplasmic reticulum nucleus

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth C], B [auth D]	IPR036300	Mir domain superfamily	Homologous Superfamily
A [auth C], B [auth D]	IPR000699	RIH domain	Domain
A [auth C], B [auth D]	IPR013662	RyR/IP3R Homology associated domain	Domain
A [auth C], B [auth D]	IPR016093	MIR motif	Domain
A [auth C], B [auth D]	IPR013320	Concanavalin A-like lectin/glucanase domain superfamily	Homologous Superfamily
A [auth C], B [auth D]	IPR002048	EF-hand domain	Domain
A [auth C], B [auth D]	IPR003877	SPRY domain	Domain
A [auth C], B [auth D]	IPR001870	B30.2/SPRY domain	Domain
A [auth C], B [auth D]	IPR009460	Ryanodine Receptor TM 4-6	Domain
A [auth C], B [auth D]	IPR015925	Ryanodine/Inositol 1,4,5-trisphosphate receptor	Family
A [auth C], B [auth D]	IPR048581	Ryanodine receptor, junctional solenoid domain	Domain
A [auth C], B [auth D]	IPR035910	RyR/IP3 receptor binding core, RIH domain superfamily	Homologous Superfamily
A [auth C], B [auth D]	IPR003032	Ryanodine receptor Ryr	Domain
A [auth C], B [auth D]	IPR035761	Ryanodine receptor, SPRY domain 1	Domain
A [auth C], B [auth D]	IPR014821	Inositol 1,4,5-trisphosphate/ryanodine receptor	Domain
A [auth C], B [auth D]	IPR035764	Ryanodine receptor, SPRY domain 2	Domain
A [auth C], B [auth D]	IPR013333	Ryanodine receptor	Family
A [auth C], B [auth D]	IPR043136	B30.2/SPRY domain superfamily	Homologous Superfamily
A [auth C], B [auth D]	IPR011992	EF-hand domain pair	Homologous Superfamily
A [auth C], B [auth D]	IPR005821	Ion transport domain	Domain
A [auth C], B [auth D]	IPR035762	Ryanodine receptor, SPRY domain 3	Domain
C [auth A], D [auth B]	IPR018247	EF-Hand 1, calcium-binding site	Binding Site
C [auth A], D [auth B]	IPR013787	S100/CaBP-9k-type, calcium binding, subdomain	Domain
C [auth A], D [auth B]	IPR001751	S100/Calcium binding protein 7/8-like, conserved site	Conserved Site
C [auth A], D [auth B]	IPR028486	Protein S100-A1	Family
C [auth A], D [auth B]	IPR002048	EF-hand domain	Domain
C [auth A], D [auth B]	IPR011992	EF-hand domain pair	Homologous Superfamily