2IV9

B2-appendage from AP2 in complex with Eps15 peptide

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2iv9a1	All beta proteins	Immunoglobulin-like beta-sandwich	Clathrin adaptor appendage domain	Alpha-adaptin ear subdomain-like	Beta2-adaptin AP2 ear domain, N-terminal subdomain	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d2iv9a2	Alpha and beta proteins (a+b)	Subdomain of clathrin and coatomer appendage domain	Subdomain of clathrin and coatomer appendage domain	Clathrin adaptor appendage, alpha and beta chain-specific domain	Beta2-adaptin AP2, C-terminal subdomain	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2iv9b1	All beta proteins	Immunoglobulin-like beta-sandwich	Clathrin adaptor appendage domain	Alpha-adaptin ear subdomain-like	Beta2-adaptin AP2 ear domain, N-terminal subdomain	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2iv9b2	Alpha and beta proteins (a+b)	Subdomain of clathrin and coatomer appendage domain	Subdomain of clathrin and coatomer appendage domain	Clathrin adaptor appendage, alpha and beta chain-specific domain	Beta2-adaptin AP2, C-terminal subdomain	HUMAN (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Clathrin adaptor appendage domain	8055291	3002052	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Subdomain of clathrin and coatomer appendage domain	8036006	3000382	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Clathrin adaptor appendage domain	8055291	3002052	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Subdomain of clathrin and coatomer appendage domain	8036006	3000382	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF02883	e2iv9A1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF02883	ECOD (1.6)
A	PF09066	e2iv9A2	A: a+b two layers	X: TBP-like	H: Subdomain of clathrin and coatomer appendage domain (From Topology)	T: Subdomain of clathrin and coatomer appendage domain	F: PF09066	ECOD (1.6)
B	PF02883	e2iv9B1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: PF02883	ECOD (1.6)
B	PF09066	e2iv9B2	A: a+b two layers	X: TBP-like	H: Subdomain of clathrin and coatomer appendage domain (From Topology)	T: Subdomain of clathrin and coatomer appendage domain	F: PF09066	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.40.1150	Mainly Beta	Sandwich	Immunoglobulin-like		CATH (4.3.0)
A	3.30.310.10	Alpha Beta	2-Layer Sandwich	TATA-Binding Protein	TATA-Binding Protein	CATH (4.3.0)
B	2.60.40.1150	Mainly Beta	Sandwich	Immunoglobulin-like		CATH (4.3.0)
B	3.30.310.10	Alpha Beta	2-Layer Sandwich	TATA-Binding Protein	TATA-Binding Protein	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF09066	Beta2-adaptin appendage, C-terminal sub-domain (B2-adapt-app_C)	Beta2-adaptin appendage, C-terminal sub-domain	Members of this family adopt a structure consisting of a 5 stranded beta-sheet, flanked by one alpha helix on the outer side, and by two alpha helices on the inner side. This domain is required for binding to clathrin, and its subsequent polymerisati ...	Members of this family adopt a structure consisting of a 5 stranded beta-sheet, flanked by one alpha helix on the outer side, and by two alpha helices on the inner side. This domain is required for binding to clathrin, and its subsequent polymerisation. Furthermore, a hydrophobic patch present in the domain also binds to a subset of D-phi-F/W motif-containing proteins that are bound by the alpha-adaptin appendage domain (epsin, AP180, eps15) [1].	Domain
A, B	PF02883	Adaptin C-terminal domain (Alpha_adaptinC2)	Adaptin C-terminal domain	Alpha adaptin is a heterotetramer which regulates clathrin-bud formation. The carboxyl-terminal appendage of the alpha subunit regulates translocation of endocytic accessory proteins to the bud site. This ig-fold domain is found in alpha, beta and ...	Alpha adaptin is a heterotetramer which regulates clathrin-bud formation. The carboxyl-terminal appendage of the alpha subunit regulates translocation of endocytic accessory proteins to the bud site. This ig-fold domain is found in alpha, beta and gamma adaptins.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	AP-2 COMPLEX SUBUNIT BETA-2	clathrin binding protein binding binding signal sequence binding peptide binding	synaptic vesicle endocytosis import into cell cellular localization transport synaptic vesicle cycle establishment of localization vesicle-mediated transport presynaptic endocytosis vesicle-mediated transport in synapse synaptic vesicle recycling localization establishment of localization in cell cellular process endocytosis synaptic vesicle endocytosis import into cell cellular localization transport synaptic vesicle cycle establishment of localization vesicle-mediated transport presynaptic endocytosis vesicle-mediated transport in synapse synaptic vesicle recycling localization establishment of localization in cell cellular process endocytosis developmental process heart development multicellular organismal process animal organ development ventricular septum development circulatory system development multicellular organism development cardiac septum development cardiac chamber development anatomical structure development cardiac ventricle development system development artery development aorta development vasculature development blood vessel development clathrin-dependent endocytosis receptor-mediated endocytosis receptor internalization postsynaptic neurotransmitter receptor internalization biological regulation protein-containing complex localization neurotransmitter receptor internalization regulation of postsynaptic membrane neurotransmitter receptor levels postsynaptic endocytosis regulation of biological quality protein localization establishment of protein localization nitrogen compound transport organic substance transport intracellular transport protein transport cellular macromolecule localization macromolecule localization intracellular protein transport coronary vasculature development kidney development renal system development	membrane cellular anatomical entity protein-containing complex intracellular anatomical structure membrane protein complex coated membrane clathrin coat membrane coat AP-type membrane coat adaptor complex cytoplasm clathrin adaptor complex endosome membrane membrane-bounded organelle endolysosome membrane membrane cellular anatomical entity protein-containing complex intracellular anatomical structure membrane protein complex coated membrane clathrin coat membrane coat AP-type membrane coat adaptor complex cytoplasm clathrin adaptor complex endosome membrane membrane-bounded organelle endolysosome membrane intracellular vesicle lytic vacuole endomembrane system intracellular organelle bounding membrane of organelle endolysosome vesicle membrane cytoplasmic vesicle vesicle lysosome vacuolar membrane endosome vacuole intracellular membrane-bounded organelle lytic vacuole membrane cytoplasmic vesicle membrane organelle lysosomal membrane organelle membrane clathrin-coated vesicle endocytic vesicle membrane coated vesicle endocytic vesicle clathrin-coated vesicle membrane clathrin-coated endocytic vesicle membrane clathrin-coated endocytic vesicle coated vesicle membrane cytosol cell periphery plasma membrane presynapse synapse cell junction vesicle coat AP-2 adaptor complex clathrin-coated pit clathrin vesicle coat clathrin coat of coated pit clathrin coat of endocytic vesicle plasma membrane protein complex glutamatergic synapse cytoplasmic side of plasma membrane cytoplasmic side of membrane side of membrane postsynapse
C [auth P]	EPIDERMAL GROWTH FACTOR RECEPTOR SUBSTRATE 15 ISOFORM B	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding SH3 domain binding protein binding protein domain specific binding cell adhesion molecule binding cadherin binding polyubiquitin modification-dependent protein binding modification-dependent protein binding	localization import into cell transport cellular process endocytosis establishment of localization vesicle-mediated transport receptor-mediated endocytosis of virus by host cell biological process involved in interspecies interaction between organisms viral life cycle biological process involved in interaction with host biological process involved in symbiotic interaction symbiont entry into host cell viral process localization import into cell transport cellular process endocytosis establishment of localization vesicle-mediated transport receptor-mediated endocytosis of virus by host cell biological process involved in interspecies interaction between organisms viral life cycle biological process involved in interaction with host biological process involved in symbiotic interaction symbiont entry into host cell viral process symbiont entry into host endocytosis involved in viral entry into host cell receptor-mediated endocytosis transport of virus protein localization establishment of protein localization cellular localization nitrogen compound transport organic substance transport protein transport cellular macromolecule localization macromolecule localization receptor internalization postsynaptic neurotransmitter receptor internalization biological regulation vesicle-mediated transport in synapse protein-containing complex localization neurotransmitter receptor internalization regulation of postsynaptic membrane neurotransmitter receptor levels postsynaptic endocytosis establishment of localization in cell regulation of biological quality regulation of signaling regulation of biological process signaling regulation of receptor recycling positive regulation of biological process regulation of macromolecule metabolic process receptor recycling positive regulation of metabolic process organic substance metabolic process receptor metabolic process positive regulation of receptor recycling positive regulation of macromolecule metabolic process metabolic process macromolecule metabolic process regulation of metabolic process positive regulation of signaling intracellular transport endosomal transport clathrin coat assembly cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular component organization or biogenesis vesicle organization organelle organization cell population proliferation regulation of cellular process regulation of cell population proliferation localization within membrane endocytic recycling vesicle-mediated transport to the plasma membrane Golgi to endosome transport post-Golgi vesicle-mediated transport cytosolic transport Golgi vesicle transport	protein-containing complex intracellular anatomical structure plasma membrane protein complex membrane plasma membrane membrane protein complex cellular anatomical entity coated membrane endomembrane system clathrin coat membrane coat cell periphery clathrin-coated pit cytoplasm protein-containing complex intracellular anatomical structure plasma membrane protein complex membrane plasma membrane membrane protein complex cellular anatomical entity coated membrane endomembrane system clathrin coat membrane coat cell periphery clathrin-coated pit cytoplasm clathrin coat of coated pit apical part of cell plasma membrane region apical plasma membrane basal part of cell basal plasma membrane cytosol membrane-bounded organelle intracellular organelle organelle intracellular membrane-bounded organelle inclusion body aggresome synapse cell junction glutamatergic synapse endosome membrane early endosome endosome intracellular vesicle early endosome membrane cytoplasmic vesicle membrane bounding membrane of organelle cytoplasmic vesicle vesicle membrane vesicle organelle membrane postsynapse

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR016024	Armadillo-type fold	Homologous Superfamily
A, B	IPR013037	Clathrin adaptor, beta-adaptin, appendage, Ig-like subdomain	Homologous Superfamily
A, B	IPR000225	Armadillo	Repeat
A, B	IPR026739	AP complex subunit beta	Family
A, B	IPR011989	Armadillo-like helical	Homologous Superfamily
A, B	IPR012295	TBP domain superfamily	Homologous Superfamily
A, B	IPR002553	Clathrin/coatomer adaptor, adaptin-like, N-terminal	Domain
A, B	IPR015151	Beta-adaptin appendage, C-terminal subdomain	Domain
A, B	IPR013041	Clathrin adaptor, appendage, Ig-like subdomain superfamily	Homologous Superfamily
A, B	IPR008152	Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain	Domain
A, B	IPR009028	Coatomer/calthrin adaptor appendage, C-terminal subdomain	Homologous Superfamily
A, B	IPR016342	AP-1/2/4 complex subunit beta	Family
C [auth P]	IPR000261	EH domain	Domain
C [auth P]	IPR002048	EF-hand domain	Domain
C [auth P]	IPR011992	EF-hand domain pair	Homologous Superfamily
C [auth P]	IPR018247	EF-Hand 1, calcium-binding site	Binding Site
C [auth P]	IPR003903	Ubiquitin interacting motif	Conserved Site

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: P63010	breast cancer esophageal adenocarcinoma oligodendroglioma chronic obstructive pulmonary disease head and neck cancer ovarian cancer Depressive Symptoms interstitial lung disease
C [auth P]	Pharos: P42566	primitive neuroectodermal tumor astrocytic tumor dermatomyositis large cell medulloblastoma malignant ependymoma medulloblastoma osteosarcoma pilocytic astrocytoma atypical teratoid rhabdoid tumor glioblastoma lung cancer malignant glioma non-small cell lung carcinoma ovarian cancer pituitary cancer primitive neuroectodermal tumor astrocytic tumor dermatomyositis large cell medulloblastoma malignant ependymoma medulloblastoma osteosarcoma pilocytic astrocytoma atypical teratoid rhabdoid tumor glioblastoma lung cancer malignant glioma non-small cell lung carcinoma ovarian cancer pituitary cancer cancer invasive ductal breast carcinoma lymphoplasmacytic lymphoma malignant mesothelioma oligodendroglioma Pick disease

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.