2IBG

Crystal Structure of Hedgehog Bound to the FNIII Domains of Ihog

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2ibga1	All beta proteins	Immunoglobulin-like beta-sandwich	Fibronectin type III	Fibronectin type III	Hedgehog receptor iHog	fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
A	d2ibga3	Artifacts	Tags	Tags	Tags	N-terminal Tags	fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
B	d2ibgb1	All beta proteins	Immunoglobulin-like beta-sandwich	Fibronectin type III	Fibronectin type III	Hedgehog receptor iHog	fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
B	d2ibgb3	Artifacts	Tags	Tags	Tags	N-terminal Tags	fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
C	d2ibgc1	All beta proteins	Immunoglobulin-like beta-sandwich	Fibronectin type III	Fibronectin type III	Hedgehog receptor iHog	fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
C	d2ibgc3	Artifacts	Tags	Tags	Tags	N-terminal Tags	fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
D	d2ibgd1	All beta proteins	Immunoglobulin-like beta-sandwich	Fibronectin type III	Fibronectin type III	Hedgehog receptor iHog	fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
D	d2ibgd3	Artifacts	Tags	Tags	Tags	N-terminal Tags	fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
E	d2ibge1	Alpha and beta proteins (a+b)	Hedgehog/DD-peptidase	Hedgehog/DD-peptidase	Hedgehog (development protein), N-terminal signaling domain	Hedgehog	fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
F	d2ibgf_	Alpha and beta proteins (a+b)	Hedgehog/DD-peptidase	Hedgehog/DD-peptidase	Hedgehog (development protein), N-terminal signaling domain	Hedgehog	fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
G	d2ibgg_	Alpha and beta proteins (a+b)	Hedgehog/DD-peptidase	Hedgehog/DD-peptidase	Hedgehog (development protein), N-terminal signaling domain	Hedgehog	fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)
H	d2ibgh_	Alpha and beta proteins (a+b)	Hedgehog/DD-peptidase	Hedgehog/DD-peptidase	Hedgehog (development protein), N-terminal signaling domain	Hedgehog	fruit fly (Drosophila melanogaster ) [TaxId: 7227 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Fibronectin type III	8055199	3002063	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Fibronectin type III	8055199	3002063	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Fibronectin type III	8055199	3002063	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Fibronectin type III	8055199	3002063	SCOP2B (2022-06-29)
E	SCOP2 Family	Hedgehog (development protein) N-terminal signaling domain	8030276	4001392	SCOP2 (2022-06-29)
E	SCOP2 Superfamily	Hedgehog/DD-peptidase	8042655	3001458	SCOP2 (2022-06-29)
F	SCOP2B Superfamily	Hedgehog/DD-peptidase	8042655	3001458	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Hedgehog/DD-peptidase	8042655	3001458	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Hedgehog/DD-peptidase	8042655	3001458	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	fn3	e2ibgA1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: fn3	ECOD (1.6)
B	fn3	e2ibgB1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: fn3	ECOD (1.6)
C	fn3	e2ibgC1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: fn3	ECOD (1.6)
D	fn3	e2ibgD1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: fn3	ECOD (1.6)
E	HH_signal	e2ibgE1	A: a+b two layers	X: Hedgehog/DD-peptidase (From Topology)	H: Hedgehog/DD-peptidase (From Topology)	T: Hedgehog/DD-peptidase	F: HH_signal	ECOD (1.6)
F	HH_signal	e2ibgF1	A: a+b two layers	X: Hedgehog/DD-peptidase (From Topology)	H: Hedgehog/DD-peptidase (From Topology)	T: Hedgehog/DD-peptidase	F: HH_signal	ECOD (1.6)
G	HH_signal	e2ibgG1	A: a+b two layers	X: Hedgehog/DD-peptidase (From Topology)	H: Hedgehog/DD-peptidase (From Topology)	T: Hedgehog/DD-peptidase	F: HH_signal	ECOD (1.6)
H	HH_signal	e2ibgH1	A: a+b two layers	X: Hedgehog/DD-peptidase (From Topology)	H: Hedgehog/DD-peptidase (From Topology)	T: Hedgehog/DD-peptidase	F: HH_signal	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
B	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
C	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
D	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
E	3.30.1380.10	Alpha Beta	2-Layer Sandwich	Muramoyl-pentapeptide Carboxypeptidase	domain 2	CATH (4.3.0)
F	3.30.1380.10	Alpha Beta	2-Layer Sandwich	Muramoyl-pentapeptide Carboxypeptidase	domain 2	CATH (4.3.0)
G	3.30.1380.10	Alpha Beta	2-Layer Sandwich	Muramoyl-pentapeptide Carboxypeptidase	domain 2	CATH (4.3.0)
H	3.30.1380.10	Alpha Beta	2-Layer Sandwich	Muramoyl-pentapeptide Carboxypeptidase	domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF00041	Fibronectin type III domain (fn3)	Fibronectin type III domain	-	Domain
E, F, G, H	PF01085	Hedgehog amino-terminal signalling domain (HH_signal)	Hedgehog amino-terminal signalling domain	For the carboxyl Hint module, see Pfam:PF01079. Hedgehog is a family of secreted signal molecules required for embryonic cell differentiation.	Domain
E, F, G, H	PF01079	Hint module (Hint)	Hint module	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	CG9211-PA	molecular transducer activity coreceptor activity signaling receptor activity identical protein binding protein binding binding protein homodimerization activity protein dimerization activity hedgehog family protein binding patched binding signaling receptor binding heparin binding glycosaminoglycan binding carbohydrate derivative binding molecular transducer activity coreceptor activity signaling receptor activity identical protein binding protein binding binding protein homodimerization activity protein dimerization activity hedgehog family protein binding patched binding signaling receptor binding heparin binding glycosaminoglycan binding carbohydrate derivative binding sulfur compound binding	developmental process multicellular organism development multicellular organismal process segmentation anatomical structure development pattern specification process regionalization segment specification cellular response to stimulus regulation of biological process regulation of cellular process cell surface receptor signaling pathway response to stimulus signaling developmental process multicellular organism development multicellular organismal process segmentation anatomical structure development pattern specification process regionalization segment specification cellular response to stimulus regulation of biological process regulation of cellular process cell surface receptor signaling pathway response to stimulus signaling biological regulation smoothened signaling pathway cell communication cellular process signal transduction animal organ development wing disc development imaginal disc pattern formation imaginal disc development wing disc pattern formation maintenance of protein location protein localization localization cellular localization cellular macromolecule localization macromolecule localization maintenance of protein location in extracellular region maintenance of location protein localization to extracellular region cuticle pattern formation cuticle development anterior/posterior pattern specification, imaginal disc anterior/posterior pattern specification wing disc anterior/posterior pattern formation cell adhesion cell-cell adhesion homotypic cell-cell adhesion sensory organ development eye development visual system development compound eye development sensory system development system development	membrane cellular anatomical entity cell surface cell periphery plasma membrane cell projection cytoneme plasma membrane bounded cell projection
E, F, G, H	Protein hedgehog	protein binding binding molecular function activator activity signaling receptor binding molecular function regulator activity signaling receptor regulator activity morphogen activity receptor ligand activity signaling receptor activator activity patched binding cation binding ion binding calcium ion binding metal ion binding protein binding binding molecular function activator activity signaling receptor binding molecular function regulator activity signaling receptor regulator activity morphogen activity receptor ligand activity signaling receptor activator activity patched binding cation binding ion binding calcium ion binding metal ion binding small molecule binding peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity cholesterol-protein transferase activity transferase activity	developmental process cellular developmental process cell differentiation cellular process cell fate specification cell fate commitment heart development multicellular organism development multicellular organismal process anatomical structure development animal organ development circulatory system development system development organonitrogen compound metabolic process developmental process cellular developmental process cell differentiation cellular process cell fate specification cell fate commitment heart development multicellular organism development multicellular organismal process anatomical structure development animal organ development circulatory system development system development organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process protein autoprocessing biosynthetic process proteolysis organic substance biosynthetic process protein processing metabolic process protein metabolic process macromolecule metabolic process blastoderm segmentation embryonic pattern specification segmentation pattern specification process embryo development regionalization segment polarity determination periodic partitioning regulation of biosynthetic process regulation of biological process regulation of cellular process biological regulation regulation of cellular metabolic process regulation of macromolecule metabolic process regulation of cellular biosynthetic process regulation of gene expression regulation of macromolecule biosynthetic process regulation of metabolic process digestive system development foregut morphogenesis tube development digestive tract development anatomical structure morphogenesis tube morphogenesis digestive tract morphogenesis wing disc development imaginal disc pattern formation imaginal disc development wing disc pattern formation head development head segmentation anterior head development anterior head segmentation sensory organ development eye development visual system development eye morphogenesis compound eye development compound eye morphogenesis sensory organ morphogenesis progression of morphogenetic furrow involved in compound eye morphogenesis animal organ morphogenesis sensory system development central nervous system development ventral midline development nervous system development positive chemotaxis cell chemotaxis cellular response to stimulus chemotaxis response to stimulus sexual reproduction reproductive process multicellular organismal reproductive process response to external stimulus germ cell migration gamete generation cellular response to chemical stimulus taxis response to chemical cell migration cell motility germ cell attraction locomotion pigmentation developmental pigmentation photoreceptor cell differentiation neurogenesis neuron differentiation eye photoreceptor cell differentiation generation of neurons compound eye photoreceptor cell differentiation intein-mediated protein splicing protein splicing spiracle morphogenesis, open tracheal system respiratory system development open tracheal system development negative regulation of homotypic cell-cell adhesion regulation of homotypic cell-cell adhesion regulation of cell adhesion homotypic cell-cell adhesion negative regulation of biological process negative regulation of cell adhesion cell adhesion cell-cell adhesion negative regulation of cell-cell adhesion negative regulation of cellular process regulation of cell-cell adhesion cell population proliferation regulation of cell proliferation involved in compound eye morphogenesis cell proliferation involved in compound eye morphogenesis regulation of cell population proliferation anterior/posterior pattern specification, imaginal disc anterior/posterior pattern specification anterior/posterior lineage restriction, imaginal disc imaginal disc lineage restriction protein localization regulation of localization cellular localization regulation of protein localization positive regulation of protein localization to cell surface positive regulation of biological process localization protein localization to cell surface regulation of cellular localization cellular macromolecule localization macromolecule localization positive regulation of protein localization regulation of protein localization to cell surface positive regulation of cellular process wing disc anterior/posterior pattern formation posterior head segmentation terminal cell fate specification, open tracheal system epithelial cell type specification, open tracheal system wing disc proximal/distal pattern formation proximal/distal pattern formation, imaginal disc proximal/distal pattern formation hindgut morphogenesis hindgut development self proteolysis heart formation heart morphogenesis anatomical structure formation involved in morphogenesis animal organ formation cell surface receptor signaling pathway signaling smoothened signaling pathway cell communication signal transduction cell-cell signaling cell-cell signaling involved in cell fate commitment regulation of mitotic cell cycle cell cycle regulation of cell cycle mitotic cell cycle regulation of cell motility regulation of epithelial cell migration, open tracheal system epithelial cell migration, open tracheal system tissue migration ameboidal-type cell migration regulation of cell migration epithelial cell migration regulation of multicellular organismal process regulation of epithelial cell migration epithelium migration regulation of locomotion Bolwig's organ morphogenesis Bolwig's organ development epidermis development tissue development imaginal disc morphogenesis appendage development post-embryonic appendage morphogenesis imaginal disc-derived wing morphogenesis instar larval or pupal development metamorphosis imaginal disc-derived appendage morphogenesis wing disc morphogenesis appendage morphogenesis imaginal disc-derived appendage development instar larval or pupal morphogenesis post-embryonic animal morphogenesis post-embryonic development trunk segmentation negative regulation of proteolysis regulation of protein metabolic process negative regulation of metabolic process regulation of primary metabolic process regulation of proteolysis negative regulation of protein metabolic process negative regulation of macromolecule metabolic process genital disc development analia development genital disc pattern formation genital disc anterior/posterior pattern formation gonadal mesoderm development gonad development reproductive system development sex differentiation mesenchyme development developmental process involved in reproduction development of primary sexual characteristics reproductive structure development mesoderm development leg disc development leg disc morphogenesis epithelium development morphogenesis of an epithelium larval development tissue morphogenesis instar larval development morphogenesis of larval imaginal disc epithelium imaginal disc growth developmental growth growth compartment pattern specification immune response immune system process mucosal immune response organ or tissue specific immune response labial disc development glial cell migration gliogenesis neuroblast proliferation positive regulation of nervous system development positive regulation of cell population proliferation regulation of developmental process regulation of neuroblast proliferation cell development regulation of cell differentiation regulation of neurogenesis regulation of cell development regulation of nervous system development positive regulation of developmental process positive regulation of cell differentiation positive regulation of neurogenesis regulation of neural precursor cell proliferation regulation of multicellular organismal development positive regulation of cell development positive regulation of neural precursor cell proliferation positive regulation of multicellular organismal process positive regulation of neuroblast proliferation neural precursor cell proliferation plasma membrane bounded cell projection organization cell projection organization cellular component assembly involved in morphogenesis cytoneme morphogenesis plasma membrane bounded cell projection morphogenesis plasma membrane bounded cell projection assembly cell morphogenesis cellular component organization cellular component biogenesis cell projection morphogenesis cellular anatomical entity morphogenesis cellular component assembly cytoneme assembly cellular component organization or biogenesis cell projection assembly	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cytoplasmic vesicle organelle vesicle endocytic vesicle extracellular region extracellular space intracellular anatomical structure cytoplasm cellular anatomical entity cytosol membrane-bounded organelle intracellular vesicle intracellular membrane-bounded organelle intracellular organelle cytoplasmic vesicle organelle vesicle endocytic vesicle extracellular region extracellular space cell periphery plasma membrane membrane endosome endomembrane system nucleus

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR036179	Immunoglobulin-like domain superfamily	Homologous Superfamily
A, B, C, D	IPR003599	Immunoglobulin subtype	Domain
A, B, C, D	IPR003598	Immunoglobulin subtype 2	Domain
A, B, C, D	IPR003961	Fibronectin type III	Domain
A, B, C, D	IPR013783	Immunoglobulin-like fold	Homologous Superfamily
A, B, C, D	IPR007110	Immunoglobulin-like domain	Domain
A, B, C, D	IPR036116	Fibronectin type III superfamily	Homologous Superfamily
E, F, G, H	IPR036844	Hint domain superfamily	Homologous Superfamily
E, F, G, H	IPR003586	Hint domain C-terminal	Domain
E, F, G, H	IPR003587	Hint domain N-terminal	Domain
E, F, G, H	IPR000320	Hedgehog, N-terminal signalling domain	Domain
E, F, G, H	IPR001657	Hedgehog protein	Family
E, F, G, H	IPR006141	Intein N-terminal splicing region	PTM
E, F, G, H	IPR001767	Hedgehog protein, Hint domain	Domain
E, F, G, H	IPR009045	Hedgehog signalling/DD-peptidase zinc-binding domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.