Annotations: 2HVY

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B [auth A]	d2hvya1	All beta proteins	PUA domain-like	PUA domain-like	PUA domain	Pseudouridine synthase II TruB, C-terminal domain	(Pyrococcus furiosus ) [TaxId: 2261 ],	SCOPe (2.08)
B [auth A]	d2hvya2	Alpha and beta proteins (a+b)	Pseudouridine synthase	Pseudouridine synthase	Pseudouridine synthase II TruB	Pseudouridine synthase II TruB	(Pyrococcus furiosus ) [TaxId: 2261 ],	SCOPe (2.08)
C [auth B]	d2hvyb1	All beta proteins	Reductase/isomerase/elongation factor common domain	Translation proteins	Gar1-like SnoRNP	Gar1 homolog PF1791	(Pyrococcus furiosus ) [TaxId: 2261 ],	SCOPe (2.08)
D [auth C]	d2hvyc1	Small proteins	Rubredoxin-like	Nop10-like SnoRNP	Nucleolar RNA-binding protein Nop10-like	Ribosome biogenesis protein Nop10	(Pyrococcus furiosus ) [TaxId: 2261 ],	SCOPe (2.08)
E [auth D]	d2hvyd1	Alpha and beta proteins (a+b)	Bacillus chorismate mutase-like	L30e-like	L30e/L7ae ribosomal proteins	Ribosomal protein L7ae	(Pyrococcus furiosus ) [TaxId: 2261 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B [auth A]	SCOP2B Superfamily	Pseudouridine synthase	8040330	3001360	SCOP2B (2022-06-29)
B [auth A]	SCOP2B Superfamily	PUA domain-like	8040329	3001015	SCOP2B (2022-06-29)
C [auth B]	SCOP2B Superfamily	Translational proteins	8040331	3000811	SCOP2B (2022-06-29)
D [auth C]	SCOP2B Superfamily	Nop10-like SnoRNP Znf-like	8040332	3000486	SCOP2B (2022-06-29)
E [auth D]	SCOP2 Family	L30e/L7ae ribosomal proteins	8020677	4001513	SCOP2 (2022-06-29)
E [auth D]	SCOP2 Superfamily	L30e-like	8033057	3001739	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B [auth A]	PF01472	e2hvyA1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: PUA domain	F: PF01472	ECOD (1.6)
B [auth A]	PF01509,PF08068,PF16198	e2hvyA2	A: a+b two layers	X: Alpha-beta plaits	H: Pseudouridine synthase (From Topology)	T: Pseudouridine synthase	F: PF01509,PF08068,PF16198	ECOD (1.6)
C [auth B]	PF04410	e2hvyB1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PF04410	ECOD (1.6)
D [auth C]	PF04135	e2hvyC1	A: few secondary structure elements	X: Rubredoxin-like	H: Nucleolar RNA-binding protein Nop10-like (From Topology)	T: Nucleolar RNA-binding protein Nop10-like	F: PF04135	ECOD (1.6)
E [auth D]	PF01248	e2hvyD1	A: a+b three layers	X: Bacillus chorismate mutase-like	H: L30e-like (From Topology)	T: L30e-like	F: PF01248	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B [auth A]	2.30.130.10	Mainly Beta	Roll	Archaeosine Trna-guanine Transglycosylase	Chain: A, domain 4	CATH (4.3.0)
B [auth A]	3.30.2350.10	Alpha Beta	2-Layer Sandwich	Pseudouridine synthase	Pseudouridine synthase	CATH (4.3.0)
C [auth B]	2.40.10.230	Mainly Beta	Beta Barrel	Thrombin, subunit H	Probable tRNA pseudouridine synthase domain	CATH (4.3.0)
D [auth C]	2.20.28.40	Mainly Beta	Single Sheet	Rubrerythrin, domain 2	H/ACA ribonucleoprotein complex, subunit Nop10	CATH (4.3.0)
E [auth D]	3.30.1330.30	Alpha Beta	2-Layer Sandwich	60s Ribosomal Protein L30	Chain: A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
B [auth A]	PF08068	DKCLD (NUC011) domain (DKCLD)	DKCLD (NUC011) domain	This is a TruB_N/PUA domain associated N-terminal domain of Dyskerin-like proteins [1].	Domain
B [auth A]	PF16198	tRNA pseudouridylate synthase B C-terminal domain (TruB_C_2)	tRNA pseudouridylate synthase B C-terminal domain	-	Family
B [auth A]	PF01509	TruB family pseudouridylate synthase (N terminal domain) (TruB_N)	TruB family pseudouridylate synthase (N terminal domain)	-	Family
B [auth A]	PF01472	PUA domain (PUA)	PUA domain	-	Family
C [auth B]	PF04410	Gar1/Naf1 RNA binding region (Gar1)	Gar1/Naf1 RNA binding region	Gar1 is a small nucleolar RNP that is required for pre-mRNA processing and pseudouridylation [1]. It is co-immunoprecipitated with the H/ACA families of snoRNAs. This family represents the conserved central region of Gar1. This region is necessary an ...	Gar1 is a small nucleolar RNP that is required for pre-mRNA processing and pseudouridylation [1]. It is co-immunoprecipitated with the H/ACA families of snoRNAs. This family represents the conserved central region of Gar1. This region is necessary and sufficient for normal cell growth, and specifically binds two snoRNAs snR10 and snR30. This region is also necessary for nucleolar targeting, and it is thought that the protein is co-transported to the nucleolus as part of a nucleoprotein complex [2]. In humans, Gar1 is also component of telomerase in vivo [3]. Naf1 is an essential protein that plays a role in ribosome biogenesis, modification of spliceosomal small nuclear RNAs and telomere synthesis, and is homologous to Gar1 [4].	Domain
D [auth C]	PF04135	Nucleolar RNA-binding protein, Nop10p family (Nop10p)	Nucleolar RNA-binding protein, Nop10p family	-	Family
E [auth D]	PF01248	Ribosomal protein L7Ae/L30e/S12e/Gadd45 family (Ribosomal_L7Ae)	Ribosomal protein L7Ae/L30e/S12e/Gadd45 family	This family includes: Ribosomal L7A from metazoa, Ribosomal L8-A and L8-B from fungi, 30S ribosomal protein HS6 from archaebacteria, 40S ribosomal protein S12 from eukaryotes, Ribosomal protein L30 from eukaryotes and archaebacteria. Gadd45 and MyD11 ...	This family includes: Ribosomal L7A from metazoa, Ribosomal L8-A and L8-B from fungi, 30S ribosomal protein HS6 from archaebacteria, 40S ribosomal protein S12 from eukaryotes, Ribosomal protein L30 from eukaryotes and archaebacteria. Gadd45 and MyD118 [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth E]	H/ACA RNA	-	-	-
B [auth A]	Probable tRNA pseudouridine synthase B	isomerase activity tRNA pseudouridine synthase activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid pseudouridine synthase activity catalytic activity, acting on a tRNA catalytic activity intramolecular transferase activity RNA binding binding organic cyclic compound binding nucleic acid binding	cellular biosynthetic process RNA metabolic process nucleobase-containing compound metabolic process organic substance metabolic process tRNA processing organic substance biosynthetic process RNA processing pseudouridine synthesis tRNA pseudouridine synthesis cellular metabolic process tRNA modification primary metabolic process gene expression macromolecule biosynthetic process cellular biosynthetic process RNA metabolic process nucleobase-containing compound metabolic process organic substance metabolic process tRNA processing organic substance biosynthetic process RNA processing pseudouridine synthesis tRNA pseudouridine synthesis cellular metabolic process tRNA modification primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process organic cyclic compound metabolic process RNA modification biosynthetic process macromolecule modification ncRNA metabolic process nucleic acid catabolic process metabolic process cellular process macromolecule metabolic process	-
C [auth B]	Small nucleolar rnp similar to gar1	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding	pseudouridine synthesis RNA metabolic process primary metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process RNA modification organic substance metabolic process macromolecule modification metabolic process macromolecule metabolic process cellular component biogenesis ribosome biogenesis ribonucleoprotein complex biogenesis pseudouridine synthesis RNA metabolic process primary metabolic process nucleic acid metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process RNA modification organic substance metabolic process macromolecule modification metabolic process macromolecule metabolic process cellular component biogenesis ribosome biogenesis ribonucleoprotein complex biogenesis cellular process cellular component organization or biogenesis	-
D [auth C]	Ribosome biogenesis protein Nop10	RNA binding binding organic cyclic compound binding snoRNA binding nucleic acid binding	ribonucleoprotein complex biogenesis cellular biosynthetic process RNA metabolic process rRNA processing nucleobase-containing compound metabolic process cellular component biogenesis ribosome biogenesis organic substance metabolic process organic substance biosynthetic process cellular component organization or biogenesis RNA processing rRNA metabolic process cellular metabolic process primary metabolic process ribonucleoprotein complex biogenesis cellular biosynthetic process RNA metabolic process rRNA processing nucleobase-containing compound metabolic process cellular component biogenesis ribosome biogenesis organic substance metabolic process organic substance biosynthetic process cellular component organization or biogenesis RNA processing rRNA metabolic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process organic cyclic compound metabolic process biosynthetic process ncRNA metabolic process nucleic acid catabolic process metabolic process cellular process macromolecule metabolic process pseudouridine synthesis RNA modification macromolecule modification	protein-containing complex ribonucleoprotein complex
E [auth D]	50S ribosomal protein L7Ae	RNA binding binding organic cyclic compound binding rRNA binding nucleic acid binding tRNA-specific ribonuclease activity endonuclease activity RNA endonuclease activity, producing 5'-phosphomonoesters catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity catalytic activity, acting on a tRNA nuclease activity RNA binding binding organic cyclic compound binding rRNA binding nucleic acid binding tRNA-specific ribonuclease activity endonuclease activity RNA endonuclease activity, producing 5'-phosphomonoesters catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity catalytic activity, acting on a tRNA nuclease activity hydrolase activity, acting on ester bonds RNA endonuclease activity ribonuclease P activity endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters catalytic activity structural molecule activity structural constituent of ribosome	cellular biosynthetic process RNA metabolic process nucleobase-containing compound metabolic process organic substance metabolic process tRNA processing organic substance biosynthetic process RNA processing tRNA 5'-end processing cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process cellular biosynthetic process RNA metabolic process nucleobase-containing compound metabolic process organic substance metabolic process tRNA processing organic substance biosynthetic process RNA processing tRNA 5'-end processing cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process organic cyclic compound metabolic process biosynthetic process ncRNA metabolic process nucleic acid catabolic process RNA 5'-end processing metabolic process cellular process macromolecule metabolic process tRNA 5'-leader removal ribonucleoprotein complex biogenesis rRNA processing cellular component biogenesis ribosome biogenesis cellular component organization or biogenesis rRNA metabolic process organonitrogen compound metabolic process organonitrogen compound biosynthetic process translation protein metabolic process	protein-containing complex ribonucleoprotein complex intracellular anatomical structure cytoplasm cellular anatomical entity intracellular organelle organelle intracellular non-membrane-bounded organelle ribosome non-membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
B [auth A]	IPR015947	PUA-like superfamily	Homologous Superfamily
B [auth A]	IPR020103	Pseudouridine synthase, catalytic domain superfamily	Homologous Superfamily
B [auth A]	IPR004521	Uncharacterised domain CHP00451	Domain
B [auth A]	IPR002501	Pseudouridine synthase II, N-terminal	Domain
B [auth A]	IPR026326	Probable tRNA pseudouridine synthase B, archaeal	Family
B [auth A]	IPR032819	tRNA pseudouridylate synthase B, C-terminal	Domain
B [auth A]	IPR036974	PUA domain superfamily	Homologous Superfamily
B [auth A]	IPR012960	Dyskerin-like	Domain
B [auth A]	IPR002478	PUA domain	Domain
B [auth A]	IPR004802	tRNA pseudouridine synthase B family	Family
C [auth B]	IPR038664	H/ACA RNP complex subunit Gar1/Naf1, Cbf5-binding domain	Homologous Superfamily
C [auth B]	IPR007504	H/ACA ribonucleoprotein complex, subunit Gar1/Naf1	Family
C [auth B]	IPR009000	Translation protein, beta-barrel domain superfamily	Homologous Superfamily
D [auth C]	IPR023532	H/ACA ribonucleoprotein complex subunit Nop10 , archaeal-type	Family
D [auth C]	IPR007264	H/ACA ribonucleoprotein complex, subunit Nop10	Family
D [auth C]	IPR036756	H/ACA ribonucleoprotein complex, subunit Nop10 superfamily	Homologous Superfamily
E [auth D]	IPR004038	Ribosomal protein eL8/eL30/eS12/Gadd45	Domain
E [auth D]	IPR022481	Large ribosomal subunit protein eL8, archaea	Family
E [auth D]	IPR004037	Large ribosomal subunit protein eL8-like, conserved site	Conserved Site
E [auth D]	IPR029064	Ribosomal protein eL30-like superfamily	Homologous Superfamily
E [auth D]	IPR018492	Ribosomal protein eL8/Nhp2 family	Family