2HTM

Crystal structure of TTHA0676 from Thermus thermophilus HB8

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2htma_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	ThiG-like	automated matches	automated matches	(Thermus thermophilus ) [TaxId: 274 ],	SCOPe (2.08)
B	d2htmb_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	ThiG-like	automated matches	automated matches	(Thermus thermophilus ) [TaxId: 274 ],	SCOPe (2.08)
D	d2htmd_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	ThiG-like	automated matches	automated matches	(Thermus thermophilus ) [TaxId: 274 ],	SCOPe (2.08)
C	d2htmc_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	ThiG-like	automated matches	automated matches	(Thermus thermophilus ) [TaxId: 274 ],	SCOPe (2.08)
E	d2htme_	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	MoaD/ThiS	ThiS	Uncharacterised protein TTHA0675	(Thermus thermophilus ) [TaxId: 274 ],	SCOPe (2.08)
F	d2htmf_	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	MoaD/ThiS	ThiS	Uncharacterised protein TTHA0675	(Thermus thermophilus ) [TaxId: 274 ],	SCOPe (2.08)
G	d2htmg_	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	MoaD/ThiS	ThiS	Uncharacterised protein TTHA0675	(Thermus thermophilus ) [TaxId: 274 ],	SCOPe (2.08)
H	d2htmh_	Alpha and beta proteins (a+b)	beta-Grasp (ubiquitin-like)	MoaD/ThiS	ThiS	Uncharacterised protein TTHA0675	(Thermus thermophilus ) [TaxId: 274 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	ThiG-like	8064399	4001971	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	ThiG-like	8064400	3000645	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	ThiG-like	8064400	3000645	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	ThiG-like	8064400	3000645	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	ThiG-like	8064400	3000645	SCOP2B (2022-06-29)
E	SCOP2 Family	ThiS	8101668	4000981	SCOP2 (2022-06-29)
E	SCOP2 Superfamily	MoaD/ThiS-like	8101669	3000330	SCOP2 (2022-06-29)
F	SCOP2B Superfamily	MoaD/ThiS-like	8101669	3000330	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	MoaD/ThiS-like	8101669	3000330	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	MoaD/ThiS-like	8101669	3000330	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	ThiG	e2htmA1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: ThiG	ECOD (1.6)
B	ThiG	e2htmB1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: ThiG	ECOD (1.6)
D	ThiG	e2htmD1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: ThiG	ECOD (1.6)
C	ThiG	e2htmC1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: ThiG	ECOD (1.6)
E	ThiS	e2htmE1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ThiS	ECOD (1.6)
F	ThiS	e2htmF1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ThiS	ECOD (1.6)
G	ThiS	e2htmG1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ThiS	ECOD (1.6)
H	ThiS	e2htmH1	A: a+b two layers	X: beta-Grasp	H: Ubiquitin-related	T: Ubiquitin-like	F: ThiS	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
B	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
D	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
C	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
E	3.10.20.30	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Beta-grasp domain	CATH (4.3.0)
F	3.10.20.30	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Beta-grasp domain	CATH (4.3.0)
G	3.10.20.30	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Beta-grasp domain	CATH (4.3.0)
H	3.10.20.30	Alpha Beta	Roll	Ubiquitin-like (UB roll)	Beta-grasp domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF05690	Thiazole biosynthesis protein ThiG (ThiG)	Thiazole biosynthesis protein ThiG	This family consists of several bacterial thiazole biosynthesis protein G sequences. ThiG , together with ThiF and ThiH, is proposed to be involved in the synthesis of 4-methyl-5-(b-hydroxyethyl)thiazole (THZ) which is an intermediate in the thiazole ...	This family consists of several bacterial thiazole biosynthesis protein G sequences. ThiG , together with ThiF and ThiH, is proposed to be involved in the synthesis of 4-methyl-5-(b-hydroxyethyl)thiazole (THZ) which is an intermediate in the thiazole production pathway [1]. This family also includes triosephosphate isomerase and pyridoxal 5'-phosphate synthase subunit PdxS.	Domain
E, F, G, H	PF02597	ThiS family (ThiS)	ThiS family	ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer Swiss:O32583. ThiS is coded in the thiCEFSGH operon in E. coli. This family of proteins have two conserved Glycines at the COOH terminus. Thiocarboxylate is formed at the last G in the a ...	ThiS (thiaminS) is a 66 aa protein involved in sulphur transfer Swiss:O32583. ThiS is coded in the thiCEFSGH operon in E. coli. This family of proteins have two conserved Glycines at the COOH terminus. Thiocarboxylate is formed at the last G in the activation process. Sulphur is transferred from ThiI to ThiS in a reaction catalysed by IscS [1]. MoaD, Swiss:P30748 a protein involved sulphur transfer in molybdopterin synthesis, is about the same length and shows limited sequence similarity to ThiS. Both have the conserved GG at the COOH end.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Thiazole biosynthesis protein thiG	thiazole synthase activity sulfurtransferase activity transferase activity, transferring sulphur-containing groups transferase activity catalytic activity	cellular biosynthetic process organonitrogen compound biosynthetic process thiamine-containing compound metabolic process vitamin biosynthetic process organic substance metabolic process organic substance biosynthetic process phosphate-containing compound metabolic process thiamine diphosphate metabolic process small molecule metabolic process sulfur compound metabolic process pyrimidine-containing compound biosynthetic process water-soluble vitamin metabolic process phosphorus metabolic process pyrimidine-containing compound metabolic process cellular biosynthetic process organonitrogen compound biosynthetic process thiamine-containing compound metabolic process vitamin biosynthetic process organic substance metabolic process organic substance biosynthetic process phosphate-containing compound metabolic process thiamine diphosphate metabolic process small molecule metabolic process sulfur compound metabolic process pyrimidine-containing compound biosynthetic process water-soluble vitamin metabolic process phosphorus metabolic process pyrimidine-containing compound metabolic process organonitrogen compound metabolic process water-soluble vitamin biosynthetic process small molecule biosynthetic process cellular metabolic process vitamin metabolic process organic cyclic compound metabolic process sulfur compound biosynthetic process biosynthetic process organophosphate metabolic process organophosphate biosynthetic process organic cyclic compound biosynthetic process metabolic process cellular process thiamine diphosphate biosynthetic process thiamine-containing compound biosynthetic process	intracellular anatomical structure cytoplasm cellular anatomical entity
E, F, G, H	Putative thiamine biosynthesis protein ThiS	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR013785	Aldolase-type TIM barrel	Homologous Superfamily
A, B, C, D	IPR033983	Thiazole synthase ThiG	Domain
A, B, C, D	IPR008867	Thiazole synthase	Family
E, F, G, H	IPR003749	Sulfur carrier ThiS/MoaD-like	Family
E, F, G, H	IPR016155	Molybdopterin synthase/thiamin biosynthesis sulphur carrier, beta-grasp	Homologous Superfamily
E, F, G, H	IPR012675	Beta-grasp domain superfamily	Homologous Superfamily
E, F, G, H	IPR010035	ThiS, thiamine-biosynthesis	Family

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.