2HE5

Crystal structure of 17alpha-hydroxysteroid dehydrogenase in binary complex with NADP(H) in an open conformation

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C	d2he5c_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	NAD(P)-linked oxidoreductase	Aldo-keto reductases (NADP)	automated matches	house mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
D	d2he5d_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	NAD(P)-linked oxidoreductase	Aldo-keto reductases (NADP)	automated matches	house mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
A	d2he5a_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	NAD(P)-linked oxidoreductase	Aldo-keto reductases (NADP)	automated matches	house mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
B	d2he5b_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	NAD(P)-linked oxidoreductase	Aldo-keto reductases (NADP)	automated matches	house mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C	SCOP2B Superfamily	NAD(P)-linked oxidoreductase	8062005	3000288	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	NAD(P)-linked oxidoreductase	8062005	3000288	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	NAD(P)-linked oxidoreductase	8062005	3000288	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	NAD(P)-linked oxidoreductase	8062005	3000288	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C	Aldo_ket_red	e2he5C1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Aldo_ket_red	ECOD (1.6)
D	Aldo_ket_red	e2he5D1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Aldo_ket_red	ECOD (1.6)
A	Aldo_ket_red	e2he5A1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Aldo_ket_red	ECOD (1.6)
B	Aldo_ket_red	e2he5B1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Aldo_ket_red	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C	3.20.20.100	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	NADP-dependent oxidoreductase domain	CATH (4.3.0)
D	3.20.20.100	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	NADP-dependent oxidoreductase domain	CATH (4.3.0)
A	3.20.20.100	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	NADP-dependent oxidoreductase domain	CATH (4.3.0)
B	3.20.20.100	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	NADP-dependent oxidoreductase domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF00248	Aldo/keto reductase family (Aldo_ket_red)	Aldo/keto reductase family	This family includes a number of K+ ion channel beta chain regulatory domains - these are reported to have oxidoreductase activity [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Aldo-keto reductase family 1, member C21	aldo-keto reductase (NADPH) activity oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors alcohol dehydrogenase (NADP+) activity catalytic activity aldose reductase (NADPH) activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity oxidoreductase activity, acting on the CH-CH group of donors bile acid binding organic acid binding binding ion binding aldo-keto reductase (NADPH) activity oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors alcohol dehydrogenase (NADP+) activity catalytic activity aldose reductase (NADPH) activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity oxidoreductase activity, acting on the CH-CH group of donors bile acid binding organic acid binding binding ion binding anion binding carboxylic acid binding monocarboxylic acid binding small molecule binding chlordecone reductase activity steroid dehydrogenase activity 17-beta-ketosteroid reductase activity steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor androsterone dehydrogenase activity androsterone dehydrogenase (B-specific) activity monooxygenase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen ketosteroid monooxygenase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen 5alpha-androstane-3beta,17beta-diol dehydrogenase activity oxidoreductase activity, acting on NAD(P)H oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor NADP+ binding nucleotide binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding adenyl nucleotide binding heterocyclic compound binding NADP binding lithocholic acid binding steroid binding lipid binding NADPH binding oxidoreductase activity, acting on the aldehyde or oxo group of donors retinal dehydrogenase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor phenanthrene 9,10-monooxygenase activity 17-beta-hydroxysteroid dehydrogenase (NADP+) activity	glycosyl compound metabolic process daunorubicin metabolic process polyketide metabolic process cellular metabolic process glycoside metabolic process carbohydrate derivative metabolic process secondary metabolic process organic cyclic compound metabolic process cellular ketone metabolic process organic substance metabolic process aminoglycoside antibiotic metabolic process small molecule metabolic process metabolic process cellular process glycosyl compound metabolic process daunorubicin metabolic process polyketide metabolic process cellular metabolic process glycoside metabolic process carbohydrate derivative metabolic process secondary metabolic process organic cyclic compound metabolic process cellular ketone metabolic process organic substance metabolic process aminoglycoside antibiotic metabolic process small molecule metabolic process metabolic process cellular process steroid metabolic process lipid biosynthetic process primary metabolic process biosynthetic process organic cyclic compound biosynthetic process organic substance biosynthetic process steroid biosynthetic process lipid metabolic process cellular response to stimulus response to organic substance response to stimulus response to lipid response to jasmonic acid response to fatty acid response to endogenous stimulus response to oxygen-containing compound cellular response to oxygen-containing compound cellular response to fatty acid cellular response to endogenous stimulus cellular response to chemical stimulus cellular response to jasmonic acid stimulus response to hormone response to chemical cellular response to lipid cellular response to organic substance cellular response to hormone stimulus organic hydroxy compound metabolic process alcohol metabolic process doxorubicin metabolic process tertiary alcohol metabolic process primary alcohol metabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR020471	Aldo-keto reductase	Family
A, B, C, D	IPR044482	Aldo-keto reductase family 1 member C	Family
A, B, C, D	IPR036812	NADP-dependent oxidoreductase domain superfamily	Homologous Superfamily
A, B, C, D	IPR018170	Aldo/keto reductase, conserved site	Conserved Site
A, B, C, D	IPR023210	NADP-dependent oxidoreductase domain	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.