2GJW

RNA Recognition and Cleavage by an Splicing Endonuclease

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
G [auth A]	d2gjwa1	Alpha and beta proteins (a/b)	Restriction endonuclease-like	tRNA-intron endonuclease catalytic domain-like	tRNA-intron endonuclease catalytic domain-like	Dimeric tRNA splicing endonuclease, domains 2 and 4	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
G [auth A]	d2gjwa3	Alpha and beta proteins (a+b)	MutS N-terminal domain-like	tRNA-intron endonuclease N-terminal domain-like	tRNA-intron endonuclease N-terminal domain-like	Dimeric tRNA splicing endonuclease, domains 1 and 3	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
G [auth A]	d2gjwa5	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
I [auth C]	d2gjwc1	Alpha and beta proteins (a/b)	Restriction endonuclease-like	tRNA-intron endonuclease catalytic domain-like	tRNA-intron endonuclease catalytic domain-like	Dimeric tRNA splicing endonuclease, domains 2 and 4	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
I [auth C]	d2gjwc3	Alpha and beta proteins (a+b)	MutS N-terminal domain-like	tRNA-intron endonuclease N-terminal domain-like	tRNA-intron endonuclease N-terminal domain-like	Dimeric tRNA splicing endonuclease, domains 1 and 3	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
I [auth C]	d2gjwc5	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
J [auth D]	d2gjwd1	Alpha and beta proteins (a/b)	Restriction endonuclease-like	tRNA-intron endonuclease catalytic domain-like	tRNA-intron endonuclease catalytic domain-like	Dimeric tRNA splicing endonuclease, domains 2 and 4	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
J [auth D]	d2gjwd3	Alpha and beta proteins (a+b)	MutS N-terminal domain-like	tRNA-intron endonuclease N-terminal domain-like	tRNA-intron endonuclease N-terminal domain-like	Dimeric tRNA splicing endonuclease, domains 1 and 3	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
H [auth B]	d2gjwb1	Alpha and beta proteins (a/b)	Restriction endonuclease-like	tRNA-intron endonuclease catalytic domain-like	tRNA-intron endonuclease catalytic domain-like	Dimeric tRNA splicing endonuclease, domains 2 and 4	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
H [auth B]	d2gjwb3	Alpha and beta proteins (a+b)	MutS N-terminal domain-like	tRNA-intron endonuclease N-terminal domain-like	tRNA-intron endonuclease N-terminal domain-like	Dimeric tRNA splicing endonuclease, domains 1 and 3	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
H [auth B]	d2gjwb5	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
G [auth A]	SCOP2B Superfamily	tRNA-intron endonuclease N-terminal domain-like	8040172	3001683	SCOP2B (2022-06-29)
G [auth A]	SCOP2B Superfamily	tRNA-intron endonuclease catalytic domain-like	8040168	3000437	SCOP2B (2022-06-29)
G [auth A]	SCOP2B Superfamily	tRNA-intron endonuclease catalytic domain-like	8040170	3000437	SCOP2B (2022-06-29)
I [auth C]	SCOP2B Superfamily	tRNA-intron endonuclease N-terminal domain-like	8040172	3001683	SCOP2B (2022-06-29)
I [auth C]	SCOP2B Superfamily	tRNA-intron endonuclease catalytic domain-like	8040170	3000437	SCOP2B (2022-06-29)
I [auth C]	SCOP2B Superfamily	tRNA-intron endonuclease catalytic domain-like	8040168	3000437	SCOP2B (2022-06-29)
J [auth D]	SCOP2B Superfamily	tRNA-intron endonuclease catalytic domain-like	8040168	3000437	SCOP2B (2022-06-29)
J [auth D]	SCOP2B Superfamily	tRNA-intron endonuclease catalytic domain-like	8040170	3000437	SCOP2B (2022-06-29)
J [auth D]	SCOP2B Superfamily	tRNA-intron endonuclease N-terminal domain-like	8040172	3001683	SCOP2B (2022-06-29)
H [auth B]	SCOP2B Superfamily	tRNA-intron endonuclease catalytic domain-like	8040168	3000437	SCOP2B (2022-06-29)
H [auth B]	SCOP2B Superfamily	tRNA-intron endonuclease N-terminal domain-like	8040172	3001683	SCOP2B (2022-06-29)
H [auth B]	SCOP2B Superfamily	tRNA-intron endonuclease catalytic domain-like	8040170	3000437	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
G [auth A]	F_UNCLASSIFIED	e2gjwA2	A: a+b two layers	X: Homing endonucleases-like	H: tRNA-intron endonuclease N-terminal domain-like (From Topology)	T: tRNA-intron endonuclease N-terminal domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
G [auth A]	PF02778	e2gjwA1	A: a+b two layers	X: Homing endonucleases-like	H: tRNA-intron endonuclease N-terminal domain-like (From Topology)	T: tRNA-intron endonuclease N-terminal domain-like	F: PF02778	ECOD (1.6)
G [auth A]	F_UNCLASSIFIED	e2gjwA3	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: tRNA-intron endonuclease catalytic domain-like (From Topology)	T: tRNA-intron endonuclease catalytic domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
G [auth A]	PF01974	e2gjwA4	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: tRNA-intron endonuclease catalytic domain-like (From Topology)	T: tRNA-intron endonuclease catalytic domain-like	F: PF01974	ECOD (1.6)
I [auth C]	F_UNCLASSIFIED	e2gjwC2	A: a+b two layers	X: Homing endonucleases-like	H: tRNA-intron endonuclease N-terminal domain-like (From Topology)	T: tRNA-intron endonuclease N-terminal domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
I [auth C]	PF02778	e2gjwC1	A: a+b two layers	X: Homing endonucleases-like	H: tRNA-intron endonuclease N-terminal domain-like (From Topology)	T: tRNA-intron endonuclease N-terminal domain-like	F: PF02778	ECOD (1.6)
I [auth C]	F_UNCLASSIFIED	e2gjwC3	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: tRNA-intron endonuclease catalytic domain-like (From Topology)	T: tRNA-intron endonuclease catalytic domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
I [auth C]	PF01974	e2gjwC4	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: tRNA-intron endonuclease catalytic domain-like (From Topology)	T: tRNA-intron endonuclease catalytic domain-like	F: PF01974	ECOD (1.6)
J [auth D]	F_UNCLASSIFIED	e2gjwD2	A: a+b two layers	X: Homing endonucleases-like	H: tRNA-intron endonuclease N-terminal domain-like (From Topology)	T: tRNA-intron endonuclease N-terminal domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
J [auth D]	PF02778	e2gjwD1	A: a+b two layers	X: Homing endonucleases-like	H: tRNA-intron endonuclease N-terminal domain-like (From Topology)	T: tRNA-intron endonuclease N-terminal domain-like	F: PF02778	ECOD (1.6)
J [auth D]	F_UNCLASSIFIED	e2gjwD3	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: tRNA-intron endonuclease catalytic domain-like (From Topology)	T: tRNA-intron endonuclease catalytic domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
J [auth D]	PF01974	e2gjwD4	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: tRNA-intron endonuclease catalytic domain-like (From Topology)	T: tRNA-intron endonuclease catalytic domain-like	F: PF01974	ECOD (1.6)
H [auth B]	F_UNCLASSIFIED	e2gjwB2	A: a+b two layers	X: Homing endonucleases-like	H: tRNA-intron endonuclease N-terminal domain-like (From Topology)	T: tRNA-intron endonuclease N-terminal domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
H [auth B]	PF02778	e2gjwB1	A: a+b two layers	X: Homing endonucleases-like	H: tRNA-intron endonuclease N-terminal domain-like (From Topology)	T: tRNA-intron endonuclease N-terminal domain-like	F: PF02778	ECOD (1.6)
H [auth B]	F_UNCLASSIFIED	e2gjwB3	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: tRNA-intron endonuclease catalytic domain-like (From Topology)	T: tRNA-intron endonuclease catalytic domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
H [auth B]	PF01974	e2gjwB4	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: tRNA-intron endonuclease catalytic domain-like (From Topology)	T: tRNA-intron endonuclease catalytic domain-like	F: PF01974	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
G [auth A]	3.40.1350.150	Alpha Beta	3-Layer(aba) Sandwich	Trna Endonuclease	Chain: A, domain 1	CATH (4.3.0)
G [auth A]	3.40.1170.20	Alpha Beta	3-Layer(aba) Sandwich	MutS, DNA mismatch repair protein, domain I	tRNA intron endonuclease, N-terminal domain	CATH (4.3.0)
G [auth A]	3.40.1350.10	Alpha Beta	3-Layer(aba) Sandwich	Trna Endonuclease	Chain: A, domain 1	CATH (4.3.0)
I [auth C]	3.40.1350.150	Alpha Beta	3-Layer(aba) Sandwich	Trna Endonuclease	Chain: A, domain 1	CATH (4.3.0)
I [auth C]	3.40.1170.20	Alpha Beta	3-Layer(aba) Sandwich	MutS, DNA mismatch repair protein, domain I	tRNA intron endonuclease, N-terminal domain	CATH (4.3.0)
I [auth C]	3.40.1350.10	Alpha Beta	3-Layer(aba) Sandwich	Trna Endonuclease	Chain: A, domain 1	CATH (4.3.0)
J [auth D]	3.40.1350.150	Alpha Beta	3-Layer(aba) Sandwich	Trna Endonuclease	Chain: A, domain 1	CATH (4.3.0)
J [auth D]	3.40.1170.20	Alpha Beta	3-Layer(aba) Sandwich	MutS, DNA mismatch repair protein, domain I	tRNA intron endonuclease, N-terminal domain	CATH (4.3.0)
J [auth D]	3.40.1350.10	Alpha Beta	3-Layer(aba) Sandwich	Trna Endonuclease	Chain: A, domain 1	CATH (4.3.0)
H [auth B]	3.40.1350.150	Alpha Beta	3-Layer(aba) Sandwich	Trna Endonuclease	Chain: A, domain 1	CATH (4.3.0)
H [auth B]	3.40.1170.20	Alpha Beta	3-Layer(aba) Sandwich	MutS, DNA mismatch repair protein, domain I	tRNA intron endonuclease, N-terminal domain	CATH (4.3.0)
H [auth B]	3.40.1350.10	Alpha Beta	3-Layer(aba) Sandwich	Trna Endonuclease	Chain: A, domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
G [auth A], H [auth B], I [auth C], J [auth D]	PF02778	tRNA intron endonuclease, N-terminal domain (tRNA_int_endo_N)	tRNA intron endonuclease, N-terminal domain	Members of this family cleave pre tRNA at the 5' and 3' splice sites to release the intron EC:3.1.27.9.	Domain
G [auth A], H [auth B], I [auth C], J [auth D]	PF01974	tRNA intron endonuclease, catalytic C-terminal domain (tRNA_int_endo)	tRNA intron endonuclease, catalytic C-terminal domain	Members of this family cleave pre tRNA at the 5' and 3' splice sites to release the intron EC:3.1.27.9.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth E], D [auth I]	5'-R(GPCPGPAPCPCPGPAPCPCPAP(DU)PAPGPCPUPGPCP*A)-3'	-	-	-
B [auth F], E [auth J]	5'-R(UPGPCPAPGPCPGPGPUPCPAP(A23))-3'	-	-	-
C [auth H], F [auth L]	5'-R(APGPGPUPCPGP*C)-3'	-	-	-
G [auth A], H [auth B], I [auth C], J [auth D]	tRNA-splicing endonuclease	endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters tRNA-specific ribonuclease activity endonuclease activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity catalytic activity, acting on a tRNA RNA endonuclease activity, producing 3'-phosphomonoesters tRNA-intron endonuclease activity nuclease activity hydrolase activity, acting on ester bonds RNA endonuclease activity catalytic activity endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters tRNA-specific ribonuclease activity endonuclease activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid RNA nuclease activity hydrolase activity catalytic activity, acting on a tRNA RNA endonuclease activity, producing 3'-phosphomonoesters tRNA-intron endonuclease activity nuclease activity hydrolase activity, acting on ester bonds RNA endonuclease activity catalytic activity lyase activity binding organic cyclic compound binding nucleic acid binding	cellular biosynthetic process RNA metabolic process nucleobase-containing compound metabolic process tRNA splicing, via endonucleolytic cleavage and ligation organic substance metabolic process tRNA processing organic substance biosynthetic process RNA processing RNA splicing cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process cellular biosynthetic process RNA metabolic process nucleobase-containing compound metabolic process tRNA splicing, via endonucleolytic cleavage and ligation organic substance metabolic process tRNA processing organic substance biosynthetic process RNA processing RNA splicing cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process organic cyclic compound metabolic process biosynthetic process RNA splicing, via endonucleolytic cleavage and ligation ncRNA metabolic process nucleic acid catabolic process metabolic process cellular process macromolecule metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
G [auth A], H [auth B], I [auth C], J [auth D]	IPR006677	tRNA intron endonuclease, catalytic domain-like	Domain
G [auth A], H [auth B], I [auth C], J [auth D]	IPR036740	tRNA intron endonuclease, N-terminal domain superfamily	Homologous Superfamily
G [auth A], H [auth B], I [auth C], J [auth D]	IPR023516	tRNA-splicing endonuclease, archaeal long subfamily	Family
G [auth A], H [auth B], I [auth C], J [auth D]	IPR011856	tRNA endonuclease-like domain superfamily	Homologous Superfamily
G [auth A], H [auth B], I [auth C], J [auth D]	IPR036167	tRNA intron endonuclease, catalytic domain-like superfamily	Homologous Superfamily
G [auth A], H [auth B], I [auth C], J [auth D]	IPR006678	tRNA intron endonuclease, N-terminal	Domain
G [auth A], H [auth B], I [auth C], J [auth D]	IPR006676	tRNA-splicing endonuclease	Family

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.