2GAI

Structure of Full Length Topoisomerase I from Thermotoga maritima in triclinic crystal form

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	F_UNCLASSIFIED	e2gaiB4	A: beta barrels	X: LigT-like	H: Prokaryotic type I DNA topoisomerase beta-barrel domain (From Topology)	T: Prokaryotic type I DNA topoisomerase beta-barrel domain	F: F_UNCLASSIFIED	ECOD (1.6)
B	PF01131	e2gaiB2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF01131	ECOD (1.6)
B	PF01751	e2gaiB3	A: a/b three-layered sandwiches	X: HAD domain-like	H: HAD domain-related	T: Toprim domain	F: PF01751	ECOD (1.6)
B	F_UNCLASSIFIED	e2gaiB5	A: few secondary structure elements	X: Rubredoxin-like	H: Rubredoxin-related	T: Rubredoxin-related	F: F_UNCLASSIFIED	ECOD (1.6)
A	F_UNCLASSIFIED	e2gaiA3	A: beta barrels	X: LigT-like	H: Prokaryotic type I DNA topoisomerase beta-barrel domain (From Topology)	T: Prokaryotic type I DNA topoisomerase beta-barrel domain	F: F_UNCLASSIFIED	ECOD (1.6)
A	PF01131	e2gaiA4	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF01131	ECOD (1.6)
A	PF01751	e2gaiA2	A: a/b three-layered sandwiches	X: HAD domain-like	H: HAD domain-related	T: Toprim domain	F: PF01751	ECOD (1.6)
A	F_UNCLASSIFIED	e2gaiA5	A: few secondary structure elements	X: Rubredoxin-like	H: Rubredoxin-related	T: Rubredoxin-related	F: F_UNCLASSIFIED	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	3.40.50.140	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)
B	1.10.460.10	Mainly Alpha	Orthogonal Bundle	Topoisomerase I	domain 2	CATH (4.3.0)
B	2.70.20.10	Mainly Beta	Distorted Sandwich	Topoisomerase I	domain 3	CATH (4.3.0)
B	1.10.290.10	Mainly Alpha	Orthogonal Bundle	Topoisomerase I	domain 4	CATH (4.3.0)
A	3.40.50.140	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold		CATH (4.3.0)
A	1.10.460.10	Mainly Alpha	Orthogonal Bundle	Topoisomerase I	domain 2	CATH (4.3.0)
A	2.70.20.10	Mainly Beta	Distorted Sandwich	Topoisomerase I	domain 3	CATH (4.3.0)
A	1.10.290.10	Mainly Alpha	Orthogonal Bundle	Topoisomerase I	domain 4	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF01131	DNA topoisomerase (Topoisom_bac)	DNA topoisomerase	-	Family
A, B	PF01751	Toprim domain (Toprim)	Toprim domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	DNA topoisomerase I	DNA binding binding organic cyclic compound binding nucleic acid binding isomerase activity DNA topoisomerase type I (single strand cut, ATP-independent) activity catalytic activity, acting on DNA DNA topoisomerase activity catalytic activity, acting on a nucleic acid catalytic activity cation binding ion binding metal ion binding small molecule binding	chromosome organization primary metabolic process DNA conformation change DNA metabolic process nucleic acid metabolic process cellular component organization nucleobase-containing compound metabolic process DNA topological change organic cyclic compound metabolic process organelle organization organic substance metabolic process cellular process metabolic process cellular component organization or biogenesis chromosome organization primary metabolic process DNA conformation change DNA metabolic process nucleic acid metabolic process cellular component organization nucleobase-containing compound metabolic process DNA topological change organic cyclic compound metabolic process organelle organization organic substance metabolic process cellular process metabolic process cellular component organization or biogenesis macromolecule metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR013497	DNA topoisomerase, type IA, central	Domain
A, B	IPR003602	DNA topoisomerase, type IA, DNA-binding domain	Domain
A, B	IPR023405	DNA topoisomerase, type IA, core domain	Homologous Superfamily
A, B	IPR013825	DNA topoisomerase, type IA, central region, subdomain 2	Homologous Superfamily
A, B	IPR003601	DNA topoisomerase, type IA, domain 2	Domain
A, B	IPR028612	DNA topoisomerase I, type IA	Family
A, B	IPR000380	DNA topoisomerase, type IA	Family
A, B	IPR013826	DNA topoisomerase, type IA, central region, subdomain 3	Homologous Superfamily
A, B	IPR006171	TOPRIM domain	Domain
A, B	IPR023406	DNA topoisomerase, type IA, active site	Active Site
A, B	IPR005733	DNA topoisomerase I, bacterial-type	Family
A, B	IPR034149	DNA topoisomerase 1, TOPRIM domain	Domain
A, B	IPR013824	DNA topoisomerase, type IA, central region, subdomain 1	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.