2FFH

THE SIGNAL SEQUENCE BINDING PROTEIN FFH FROM THERMUS AQUATICUS

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2ffha1	All alpha proteins	Four-helical up-and-down bundle	Domain of the SRP/SRP receptor G-proteins	Domain of the SRP/SRP receptor G-proteins	Signal sequence recognition protein Ffh	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)
A	d2ffha2	All alpha proteins	Signal peptide-binding domain	Signal peptide-binding domain	Signal peptide-binding domain	Signal sequence binding protein Ffh	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)
A	d2ffha3	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	Nitrogenase iron protein-like	GTPase domain of the signal sequence recognition protein Ffh	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)
B	d2ffhb1	All alpha proteins	Four-helical up-and-down bundle	Domain of the SRP/SRP receptor G-proteins	Domain of the SRP/SRP receptor G-proteins	Signal sequence recognition protein Ffh	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)
B	d2ffhb2	All alpha proteins	Signal peptide-binding domain	Signal peptide-binding domain	Signal peptide-binding domain	Signal sequence binding protein Ffh	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)
B	d2ffhb3	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	Nitrogenase iron protein-like	GTPase domain of the signal sequence recognition protein Ffh	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)
C	d2ffhc1	All alpha proteins	Four-helical up-and-down bundle	Domain of the SRP/SRP receptor G-proteins	Domain of the SRP/SRP receptor G-proteins	Signal sequence recognition protein Ffh	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)
C	d2ffhc2	All alpha proteins	Signal peptide-binding domain	Signal peptide-binding domain	Signal peptide-binding domain	Signal sequence binding protein Ffh	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)
C	d2ffhc3	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	Nitrogenase iron protein-like	GTPase domain of the signal sequence recognition protein Ffh	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	SRP54-like	8089710	4003997	SCOP2 (2022-06-29)
A	SCOP2 Family	Signal peptide binding domain-like	8089713	4000906	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	SRP/SRP receptor N-terminal domain-like	8089711	3001834	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Simibi-like P-loop NTPases	8089712	3002018	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Signal peptide binding domain-like	8089714	3001939	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	SRP/SRP receptor N-terminal domain-like	8089711	3001834	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Simibi-like P-loop NTPases	8089712	3002018	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Signal peptide binding domain-like	8089714	3001939	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	SRP/SRP receptor N-terminal domain-like	8089711	3001834	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Simibi-like P-loop NTPases	8089712	3002018	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Signal peptide binding domain-like	8089714	3001939	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF02978	e2ffhA5	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: PF02978	ECOD (1.6)
A	PF02881	e2ffhA2	A: alpha bundles	X: Domain of the SRP/SRP receptor G proteins-like	H: Domain of the SRP/SRP receptor G-proteins (From Topology)	T: Domain of the SRP/SRP receptor G-proteins	F: PF02881	ECOD (1.6)
A	PF00448	e2ffhA4	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00448	ECOD (1.6)
B	PF02978	e2ffhB5	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: PF02978	ECOD (1.6)
B	PF02881	e2ffhB2	A: alpha bundles	X: Domain of the SRP/SRP receptor G proteins-like	H: Domain of the SRP/SRP receptor G-proteins (From Topology)	T: Domain of the SRP/SRP receptor G-proteins	F: PF02881	ECOD (1.6)
B	PF00448	e2ffhB4	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00448	ECOD (1.6)
C	PF02978	e2ffhC5	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: PF02978	ECOD (1.6)
C	PF02881	e2ffhC2	A: alpha bundles	X: Domain of the SRP/SRP receptor G proteins-like	H: Domain of the SRP/SRP receptor G-proteins (From Topology)	T: Domain of the SRP/SRP receptor G-proteins	F: PF02881	ECOD (1.6)
C	PF00448	e2ffhC4	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00448	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.120.140	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	SRP54, nucleotide-binding domain	CATH (4.3.0)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
A	1.10.260.30	Mainly Alpha	Orthogonal Bundle	434 Repressor (Amino-terminal Domain)	Signal recognition particle, SRP54 subunit, M-domain	CATH (4.3.0)
B	1.20.120.140	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	SRP54, nucleotide-binding domain	CATH (4.3.0)
B	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
B	1.10.260.30	Mainly Alpha	Orthogonal Bundle	434 Repressor (Amino-terminal Domain)	Signal recognition particle, SRP54 subunit, M-domain	CATH (4.3.0)
C	1.20.120.140	Mainly Alpha	Up-down Bundle	Four Helix Bundle (Hemerythrin (Met), subunit A)	SRP54, nucleotide-binding domain	CATH (4.3.0)
C	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
C	1.10.260.30	Mainly Alpha	Orthogonal Bundle	434 Repressor (Amino-terminal Domain)	Signal recognition particle, SRP54 subunit, M-domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C	PF00448	SRP54-type protein, GTPase domain (SRP54)	SRP54-type protein, GTPase domain	This family includes relatives of the G-domain of the SRP54 family of proteins.	Domain
A, B, C	PF02978	Signal peptide binding domain (SRP_SPB)	Signal peptide binding domain	-	Domain
A, B, C	PF02881	SRP54-type protein, helical bundle domain (SRP54_N)	SRP54-type protein, helical bundle domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C	PROTEIN (FFH)	RNA binding 7S RNA binding binding organic cyclic compound binding nucleic acid binding pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity ATP-dependent activity guanyl nucleotide binding RNA binding 7S RNA binding binding organic cyclic compound binding nucleic acid binding pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity ATP-dependent activity guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding GTPase activity	protein localization establishment of protein localization cellular localization establishment of protein localization to membrane protein targeting localization within membrane protein localization to membrane establishment of localization protein targeting to membrane localization SRP-dependent cotranslational protein targeting to membrane establishment of protein localization to organelle cellular macromolecule localization macromolecule localization protein localization to organelle protein localization establishment of protein localization cellular localization establishment of protein localization to membrane protein targeting localization within membrane protein localization to membrane establishment of localization protein targeting to membrane localization SRP-dependent cotranslational protein targeting to membrane establishment of protein localization to organelle cellular macromolecule localization macromolecule localization protein localization to organelle cotranslational protein targeting to membrane cellular process establishment of protein localization to endoplasmic reticulum protein localization to endoplasmic reticulum protein targeting to ER	signal recognition particle protein-containing complex intracellular anatomical structure ribonucleoprotein complex cytoplasm cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C	IPR000897	Signal recognition particle, SRP54 subunit, GTPase domain	Domain
A, B, C	IPR022941	Signal recognition particle, SRP54 subunit	Family
A, B, C	IPR036225	SRP/SRP receptor, N-terminal	Homologous Superfamily
A, B, C	IPR004125	Signal recognition particle, SRP54 subunit, M-domain	Domain
A, B, C	IPR042101	Signal recognition particle SRP54, N-terminal domain superfamily	Homologous Superfamily
A, B, C	IPR003593	AAA+ ATPase domain	Domain
A, B, C	IPR004780	Signal recognition particle protein	Family
A, B, C	IPR013822	Signal recognition particle SRP54, helical bundle	Domain
A, B, C	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B, C	IPR036891	Signal recognition particle, SRP54 subunit, M-domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.