2FB5

Structural Genomics; The crystal structure of the hypothetical membrane spanning protein from Bacillus cereus

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2fb5a1	Alpha and beta proteins (a+b)	YojJ-like	YojJ-like	YojJ-like	Hypothetical protein BC4920 (YojJ)	(Bacillus cereus ) [TaxId: 1396 ],	SCOPe (2.08)
A	d2fb5a2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Bacillus cereus ) [TaxId: 1396 ],	SCOPe (2.08)
B	d2fb5b2	Alpha and beta proteins (a+b)	YojJ-like	YojJ-like	YojJ-like	Hypothetical protein BC4920 (YojJ)	(Bacillus cereus ) [TaxId: 1396 ],	SCOPe (2.08)
B	d2fb5b3	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Bacillus cereus ) [TaxId: 1396 ],	SCOPe (2.08)
C	d2fb5c2	Alpha and beta proteins (a+b)	YojJ-like	YojJ-like	YojJ-like	Hypothetical protein BC4920 (YojJ)	(Bacillus cereus ) [TaxId: 1396 ],	SCOPe (2.08)
C	d2fb5c3	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Bacillus cereus ) [TaxId: 1396 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	DacA-like	8028356	4003884	SCOP2 (2022-06-29)
A	SCOP2 Family	DacB N-terminal region-like	8089954	4007691	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	DacA-like	8040735	3001724	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	DacB N-terminal region-like	8089955	3002407	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	DacB N-terminal region-like	8089955	3002407	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	DacA-like	8040735	3001724	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	DacA-like	8040735	3001724	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	DacB N-terminal region-like	8089955	3002407	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF02457,PF10372	e2fb5A1	A: a+b three layers	X: DisA nucleotide-binding domain (From Topology)	H: DisA nucleotide-binding domain (From Topology)	T: DisA nucleotide-binding domain	F: PF02457,PF10372	ECOD (1.6)
B	PF02457,PF10372	e2fb5B1	A: a+b three layers	X: DisA nucleotide-binding domain (From Topology)	H: DisA nucleotide-binding domain (From Topology)	T: DisA nucleotide-binding domain	F: PF02457,PF10372	ECOD (1.6)
C	PF02457,PF10372	e2fb5C1	A: a+b three layers	X: DisA nucleotide-binding domain (From Topology)	H: DisA nucleotide-binding domain (From Topology)	T: DisA nucleotide-binding domain	F: PF02457,PF10372	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.287.770	Mainly Alpha	Orthogonal Bundle	Helix Hairpins	YojJ-like	CATH (4.3.0)
A	3.40.1700.10	Alpha Beta	3-Layer(aba) Sandwich	YojJ-like (1	DNA integrity scanning protein, DisA, N-terminal domain	CATH (4.3.0)
B	1.10.287.770	Mainly Alpha	Orthogonal Bundle	Helix Hairpins	YojJ-like	CATH (4.3.0)
B	3.40.1700.10	Alpha Beta	3-Layer(aba) Sandwich	YojJ-like (1	DNA integrity scanning protein, DisA, N-terminal domain	CATH (4.3.0)
C	1.10.287.770	Mainly Alpha	Orthogonal Bundle	Helix Hairpins	YojJ-like	CATH (4.3.0)
C	3.40.1700.10	Alpha Beta	3-Layer(aba) Sandwich	YojJ-like (1	DNA integrity scanning protein, DisA, N-terminal domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C	PF10372	Diadenylate cyclase CdaS, N-terminal (CdaS_N)	Diadenylate cyclase CdaS, N-terminal	This entry represents an alpha-hairpin found at the N-terminal of Diadenylate cyclase CdaS. CdaS is one of 3 paralogous diadenylate cyclases (DAC) in Bacillus subtilis and B. thuringiensis, catalysing he condensation of 2 ATP molecules into cyclic di ...	This entry represents an alpha-hairpin found at the N-terminal of Diadenylate cyclase CdaS. CdaS is one of 3 paralogous diadenylate cyclases (DAC) in Bacillus subtilis and B. thuringiensis, catalysing he condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). It has slow DAC activity with ADP as a substrate and may have weak ADPase activity [1]. CdaS is required for efficient spore formation in B.thuringiensis, whereas in B.subtilis, it is required for efficient spore germination. It is produced under the control of different sigma factors in the two bacteria. It is also required for parasporal crystal formation [1-3].	Domain
A, B, C	PF02457	DisA bacterial checkpoint controller nucleotide-binding (DAC)	DisA bacterial checkpoint controller nucleotide-binding	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C	hypothetical Membrane Spanning Protein	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding diadenylate cyclase activity transferase activity nucleotidyltransferase activity catalytic activity transferase activity, transferring phosphorus-containing groups phosphorus-oxygen lyase activity cyclase activity lyase activity adenylate cyclase activity	ribose phosphate metabolic process nucleotide biosynthetic process organonitrogen compound biosynthetic process carbohydrate derivative metabolic process purine ribonucleotide biosynthetic process cAMP biosynthetic process cyclic nucleotide metabolic process nucleobase-containing compound metabolic process ribonucleotide biosynthetic process purine nucleotide metabolic process organic substance metabolic process ribonucleotide metabolic process nucleobase-containing small molecule metabolic process purine-containing compound biosynthetic process cyclic nucleotide biosynthetic process ribose phosphate metabolic process nucleotide biosynthetic process organonitrogen compound biosynthetic process carbohydrate derivative metabolic process purine ribonucleotide biosynthetic process cAMP biosynthetic process cyclic nucleotide metabolic process nucleobase-containing compound metabolic process ribonucleotide biosynthetic process purine nucleotide metabolic process organic substance metabolic process ribonucleotide metabolic process nucleobase-containing small molecule metabolic process purine-containing compound biosynthetic process cyclic nucleotide biosynthetic process phosphate-containing compound metabolic process organic substance biosynthetic process small molecule metabolic process phosphorus metabolic process ribose phosphate biosynthetic process nucleoside phosphate metabolic process carbohydrate derivative biosynthetic process organonitrogen compound metabolic process cAMP metabolic process cyclic purine nucleotide metabolic process cellular metabolic process primary metabolic process nucleotide metabolic process nucleobase-containing compound biosynthetic process purine-containing compound metabolic process organic cyclic compound metabolic process biosynthetic process organophosphate metabolic process organophosphate biosynthetic process organic cyclic compound biosynthetic process nucleoside phosphate biosynthetic process metabolic process cellular process purine ribonucleotide metabolic process purine nucleotide biosynthetic process	cell periphery plasma membrane membrane cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C	IPR019457	Diadenylate cyclase CdaS, N-terminal	Domain
A, B, C	IPR034693	Diadenylate cyclase CdaS	Family
A, B, C	IPR036888	DNA integrity scanning protein, DisA, N-terminal domain superfamily	Homologous Superfamily
A, B, C	IPR014046	Diadenylate cyclase	Family
A, B, C	IPR003390	DNA integrity scanning protein, DisA, N-terminal	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.