2E52

Crystal structural analysis of HindIII restriction endonuclease in complex with cognate DNA at 2.0 angstrom resolution

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C [auth B]	SCOP2B Superfamily	Restriction endonuclease-like	8054056	3000104	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Restriction endonuclease-like	8054056	3000104	SCOP2B (2022-06-29)
B [auth C]	SCOP2B Superfamily	Restriction endonuclease-like	8054056	3000104	SCOP2B (2022-06-29)
D	SCOP2 Family	HinDIII-like	8045020	4004260	SCOP2 (2022-06-29)
D	SCOP2 Superfamily	Restriction endonuclease-like	8054056	3000104	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C [auth B]	RE_HindIII	e2e52B1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: RE_HindIII	ECOD (1.6)
A	RE_HindIII	e2e52A1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: RE_HindIII	ECOD (1.6)
B [auth C]	RE_HindIII	e2e52C1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: RE_HindIII	ECOD (1.6)
D	RE_HindIII	e2e52D1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: RE_HindIII	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C [auth B]	3.40.91.70	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease	Type II restriction endonuclease, HindIII	CATH (4.3.0)
C [auth B]	6.10.250.1510	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
A	3.40.91.70	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease	Type II restriction endonuclease, HindIII	CATH (4.3.0)
A	6.10.250.1510	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
B [auth C]	3.40.91.70	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease	Type II restriction endonuclease, HindIII	CATH (4.3.0)
B [auth C]	6.10.250.1510	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
D	3.40.91.70	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease	Type II restriction endonuclease, HindIII	CATH (4.3.0)
D	6.10.250.1510	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B [auth C], C [auth B], D	PF09518	HindIII restriction endonuclease (RE_HindIII)	HindIII restriction endonuclease	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B [auth C], C [auth B], D	Type II restriction enzyme HindIII	endonuclease activity catalytic activity, acting on a nucleic acid endodeoxyribonuclease activity, producing 5'-phosphomonoesters hydrolase activity DNA nuclease activity type II site-specific deoxyribonuclease activity restriction endodeoxyribonuclease activity DNA endonuclease activity nuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on DNA endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters catalytic activity	biological process involved in interspecies interaction between organisms response to stimulus DNA restriction-modification system clearance of foreign intracellular nucleic acids response to biotic stimulus nucleobase-containing compound metabolic process DNA modification organic substance metabolic process response to stress response to external biotic stimulus response to other organism defense response defense response to symbiont response to external stimulus biological process involved in interspecies interaction between organisms response to stimulus DNA restriction-modification system clearance of foreign intracellular nucleic acids response to biotic stimulus nucleobase-containing compound metabolic process DNA modification organic substance metabolic process response to stress response to external biotic stimulus response to other organism defense response defense response to symbiont response to external stimulus primary metabolic process clearance of foreign intracellular DNA DNA metabolic process nucleic acid metabolic process organic cyclic compound metabolic process defense response to other organism macromolecule modification metabolic process macromolecule metabolic process	-
E, F, G, H, I E, F, G, H, I, J	DNA (5'-D(DGPDCPDCPDAPDAPDGPDCPDTPDTPDGPDGPDC)-3')	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B [auth C], C [auth B], D	IPR019043	Restriction endonuclease, type II, HindIII	Family
A, B [auth C], C [auth B], D	IPR038373	Restriction endonuclease, type II, HindIII superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.