2C12

Crystal Structure of Nitroalkane Oxidase in Complex with Spermine, a Competitive Inhibitor

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2c12a1	All alpha proteins	Bromodomain-like	Acyl-CoA dehydrogenase C-terminal domain-like	Medium chain acyl-CoA dehydrogenase-like, C-terminal domain	Nitroalkane oxidase	(Fusarium oxysporum ) [TaxId: 5507 ],	SCOPe (2.08)
A	d2c12a2	Multi-domain proteins (alpha and beta)	Acyl-CoA dehydrogenase NM domain-like	Acyl-CoA dehydrogenase NM domain-like	Medium chain acyl-CoA dehydrogenase, NM (N-terminal and middle) domains	Nitroalkane oxidase	(Fusarium oxysporum ) [TaxId: 5507 ],	SCOPe (2.08)
B	d2c12b1	All alpha proteins	Bromodomain-like	Acyl-CoA dehydrogenase C-terminal domain-like	Medium chain acyl-CoA dehydrogenase-like, C-terminal domain	Nitroalkane oxidase	(Fusarium oxysporum ) [TaxId: 5507 ],	SCOPe (2.08)
B	d2c12b2	Multi-domain proteins (alpha and beta)	Acyl-CoA dehydrogenase NM domain-like	Acyl-CoA dehydrogenase NM domain-like	Medium chain acyl-CoA dehydrogenase, NM (N-terminal and middle) domains	Nitroalkane oxidase	(Fusarium oxysporum ) [TaxId: 5507 ],	SCOPe (2.08)
C	d2c12c1	All alpha proteins	Bromodomain-like	Acyl-CoA dehydrogenase C-terminal domain-like	Medium chain acyl-CoA dehydrogenase-like, C-terminal domain	Nitroalkane oxidase	(Fusarium oxysporum ) [TaxId: 5507 ],	SCOPe (2.08)
C	d2c12c2	Multi-domain proteins (alpha and beta)	Acyl-CoA dehydrogenase NM domain-like	Acyl-CoA dehydrogenase NM domain-like	Medium chain acyl-CoA dehydrogenase, NM (N-terminal and middle) domains	Nitroalkane oxidase	(Fusarium oxysporum ) [TaxId: 5507 ],	SCOPe (2.08)
D	d2c12d1	All alpha proteins	Bromodomain-like	Acyl-CoA dehydrogenase C-terminal domain-like	Medium chain acyl-CoA dehydrogenase-like, C-terminal domain	Nitroalkane oxidase	(Fusarium oxysporum ) [TaxId: 5507 ],	SCOPe (2.08)
D	d2c12d2	Multi-domain proteins (alpha and beta)	Acyl-CoA dehydrogenase NM domain-like	Acyl-CoA dehydrogenase NM domain-like	Medium chain acyl-CoA dehydrogenase, NM (N-terminal and middle) domains	Nitroalkane oxidase	(Fusarium oxysporum ) [TaxId: 5507 ],	SCOPe (2.08)
E	d2c12e1	All alpha proteins	Bromodomain-like	Acyl-CoA dehydrogenase C-terminal domain-like	Medium chain acyl-CoA dehydrogenase-like, C-terminal domain	Nitroalkane oxidase	(Fusarium oxysporum ) [TaxId: 5507 ],	SCOPe (2.08)
E	d2c12e2	Multi-domain proteins (alpha and beta)	Acyl-CoA dehydrogenase NM domain-like	Acyl-CoA dehydrogenase NM domain-like	Medium chain acyl-CoA dehydrogenase, NM (N-terminal and middle) domains	Nitroalkane oxidase	(Fusarium oxysporum ) [TaxId: 5507 ],	SCOPe (2.08)
F	d2c12f1	All alpha proteins	Bromodomain-like	Acyl-CoA dehydrogenase C-terminal domain-like	Medium chain acyl-CoA dehydrogenase-like, C-terminal domain	Nitroalkane oxidase	(Fusarium oxysporum ) [TaxId: 5507 ],	SCOPe (2.08)
F	d2c12f2	Multi-domain proteins (alpha and beta)	Acyl-CoA dehydrogenase NM domain-like	Acyl-CoA dehydrogenase NM domain-like	Medium chain acyl-CoA dehydrogenase, NM (N-terminal and middle) domains	Nitroalkane oxidase	(Fusarium oxysporum ) [TaxId: 5507 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Medium chain acyl-CoA dehydrogenase C-terminal domain-like	8026676	4000821	SCOP2 (2022-06-29)
A	SCOP2 Family	Medium chain acyl-CoA dehydrogenase FAD-binding domain-like	8026677	4002751	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Acyl-CoA dehydrogenase C-terminal domain-like	8039055	3001580	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Acyl-CoA dehydrogenase N-terminal lobe-like	8039056	3001701	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Acyl-CoA dehydrogenase N-terminal lobe-like	8039056	3001701	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Acyl-CoA dehydrogenase C-terminal domain-like	8039055	3001580	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Acyl-CoA dehydrogenase C-terminal domain-like	8039055	3001580	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Acyl-CoA dehydrogenase N-terminal lobe-like	8039056	3001701	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Acyl-CoA dehydrogenase N-terminal lobe-like	8039056	3001701	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Acyl-CoA dehydrogenase C-terminal domain-like	8039055	3001580	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Acyl-CoA dehydrogenase N-terminal lobe-like	8039056	3001701	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Acyl-CoA dehydrogenase C-terminal domain-like	8039055	3001580	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Acyl-CoA dehydrogenase C-terminal domain-like	8039055	3001580	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Acyl-CoA dehydrogenase N-terminal lobe-like	8039056	3001701	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	F_UNCLASSIFIED	e2c12A1	A: beta complex topology	X: Acyl-CoA dehydrogenase middle domain-like (From Topology)	H: Acyl-CoA dehydrogenase middle domain-like (From Topology)	T: Acyl-CoA dehydrogenase middle domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
A	PF02771	e2c12A2	A: alpha arrays	X: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	H: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase N-terminal domain-like	F: PF02771	ECOD (1.6)
A	PF00441	e2c12A3	A: alpha bundles	X: Bromodomain-like	H: Acyl-CoA dehydrogenase C-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase C-terminal domain-like	F: PF00441	ECOD (1.6)
B	F_UNCLASSIFIED	e2c12B1	A: beta complex topology	X: Acyl-CoA dehydrogenase middle domain-like (From Topology)	H: Acyl-CoA dehydrogenase middle domain-like (From Topology)	T: Acyl-CoA dehydrogenase middle domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
B	PF02771	e2c12B2	A: alpha arrays	X: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	H: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase N-terminal domain-like	F: PF02771	ECOD (1.6)
B	PF00441	e2c12B3	A: alpha bundles	X: Bromodomain-like	H: Acyl-CoA dehydrogenase C-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase C-terminal domain-like	F: PF00441	ECOD (1.6)
C	F_UNCLASSIFIED	e2c12C1	A: beta complex topology	X: Acyl-CoA dehydrogenase middle domain-like (From Topology)	H: Acyl-CoA dehydrogenase middle domain-like (From Topology)	T: Acyl-CoA dehydrogenase middle domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
C	PF02771	e2c12C2	A: alpha arrays	X: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	H: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase N-terminal domain-like	F: PF02771	ECOD (1.6)
C	PF00441	e2c12C3	A: alpha bundles	X: Bromodomain-like	H: Acyl-CoA dehydrogenase C-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase C-terminal domain-like	F: PF00441	ECOD (1.6)
D	F_UNCLASSIFIED	e2c12D1	A: beta complex topology	X: Acyl-CoA dehydrogenase middle domain-like (From Topology)	H: Acyl-CoA dehydrogenase middle domain-like (From Topology)	T: Acyl-CoA dehydrogenase middle domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
D	PF02771	e2c12D2	A: alpha arrays	X: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	H: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase N-terminal domain-like	F: PF02771	ECOD (1.6)
D	PF00441	e2c12D3	A: alpha bundles	X: Bromodomain-like	H: Acyl-CoA dehydrogenase C-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase C-terminal domain-like	F: PF00441	ECOD (1.6)
E	F_UNCLASSIFIED	e2c12E1	A: beta complex topology	X: Acyl-CoA dehydrogenase middle domain-like (From Topology)	H: Acyl-CoA dehydrogenase middle domain-like (From Topology)	T: Acyl-CoA dehydrogenase middle domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
E	PF02771	e2c12E2	A: alpha arrays	X: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	H: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase N-terminal domain-like	F: PF02771	ECOD (1.6)
E	PF00441	e2c12E3	A: alpha bundles	X: Bromodomain-like	H: Acyl-CoA dehydrogenase C-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase C-terminal domain-like	F: PF00441	ECOD (1.6)
F	F_UNCLASSIFIED	e2c12F1	A: beta complex topology	X: Acyl-CoA dehydrogenase middle domain-like (From Topology)	H: Acyl-CoA dehydrogenase middle domain-like (From Topology)	T: Acyl-CoA dehydrogenase middle domain-like	F: F_UNCLASSIFIED	ECOD (1.6)
F	PF02771	e2c12F2	A: alpha arrays	X: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	H: Acyl-CoA dehydrogenase N-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase N-terminal domain-like	F: PF02771	ECOD (1.6)
F	PF00441	e2c12F3	A: alpha bundles	X: Bromodomain-like	H: Acyl-CoA dehydrogenase C-terminal domain-like (From Topology)	T: Acyl-CoA dehydrogenase C-terminal domain-like	F: PF00441	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.540.10	Mainly Alpha	Orthogonal Bundle	Butyryl-Coa Dehydrogenase, subunit A	domain 1	CATH (4.3.0)
A	2.40.110.10	Mainly Beta	Beta Barrel	Butyryl-CoA Dehydrogenase, subunit A	domain 2	CATH (4.3.0)
A	1.20.140.10	Mainly Alpha	Up-down Bundle	Butyryl-CoA Dehydrogenase, subunit A	domain 3	CATH (4.3.0)
B	1.10.540.10	Mainly Alpha	Orthogonal Bundle	Butyryl-Coa Dehydrogenase, subunit A	domain 1	CATH (4.3.0)
B	2.40.110.10	Mainly Beta	Beta Barrel	Butyryl-CoA Dehydrogenase, subunit A	domain 2	CATH (4.3.0)
B	1.20.140.10	Mainly Alpha	Up-down Bundle	Butyryl-CoA Dehydrogenase, subunit A	domain 3	CATH (4.3.0)
C	1.10.540.10	Mainly Alpha	Orthogonal Bundle	Butyryl-Coa Dehydrogenase, subunit A	domain 1	CATH (4.3.0)
C	2.40.110.10	Mainly Beta	Beta Barrel	Butyryl-CoA Dehydrogenase, subunit A	domain 2	CATH (4.3.0)
C	1.20.140.10	Mainly Alpha	Up-down Bundle	Butyryl-CoA Dehydrogenase, subunit A	domain 3	CATH (4.3.0)
D	1.10.540.10	Mainly Alpha	Orthogonal Bundle	Butyryl-Coa Dehydrogenase, subunit A	domain 1	CATH (4.3.0)
D	2.40.110.10	Mainly Beta	Beta Barrel	Butyryl-CoA Dehydrogenase, subunit A	domain 2	CATH (4.3.0)
D	1.20.140.10	Mainly Alpha	Up-down Bundle	Butyryl-CoA Dehydrogenase, subunit A	domain 3	CATH (4.3.0)
E	1.10.540.10	Mainly Alpha	Orthogonal Bundle	Butyryl-Coa Dehydrogenase, subunit A	domain 1	CATH (4.3.0)
E	2.40.110.10	Mainly Beta	Beta Barrel	Butyryl-CoA Dehydrogenase, subunit A	domain 2	CATH (4.3.0)
E	1.20.140.10	Mainly Alpha	Up-down Bundle	Butyryl-CoA Dehydrogenase, subunit A	domain 3	CATH (4.3.0)
F	1.10.540.10	Mainly Alpha	Orthogonal Bundle	Butyryl-Coa Dehydrogenase, subunit A	domain 1	CATH (4.3.0)
F	2.40.110.10	Mainly Beta	Beta Barrel	Butyryl-CoA Dehydrogenase, subunit A	domain 2	CATH (4.3.0)
F	1.20.140.10	Mainly Alpha	Up-down Bundle	Butyryl-CoA Dehydrogenase, subunit A	domain 3	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F	PF02771	Acyl-CoA dehydrogenase, N-terminal domain (Acyl-CoA_dh_N)	Acyl-CoA dehydrogenase, N-terminal domain	The N-terminal domain of Acyl-CoA dehydrogenase is an all-alpha domain.	Domain
A, B, C, D, E A, B, C, D, E, F	PF00441	Acyl-CoA dehydrogenase, C-terminal domain (Acyl-CoA_dh_1)	Acyl-CoA dehydrogenase, C-terminal domain	C-terminal domain of Acyl-CoA dehydrogenase is an all-alpha, four helical up-and-down bundle.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F	NITROALKANE OXIDASE	oxidoreductase activity nitroalkane oxidase activity oxidoreductase activity, acting on other nitrogenous compounds as donors oxidoreductase activity, acting on other nitrogenous compounds as donors, oxygen as acceptor catalytic activity FAD binding flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding nucleotide binding heterocyclic compound binding oxidoreductase activity nitroalkane oxidase activity oxidoreductase activity, acting on other nitrogenous compounds as donors oxidoreductase activity, acting on other nitrogenous compounds as donors, oxygen as acceptor catalytic activity FAD binding flavin adenine dinucleotide binding binding nucleoside phosphate binding ion binding organic cyclic compound binding anion binding nucleotide binding heterocyclic compound binding small molecule binding nitroethane oxidase activity oxidoreductase activity, acting on the CH-CH group of donors	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F	IPR009100	Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR046373	Acyl-CoA oxidase/dehydrogenase, middle domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR009075	Acyl-CoA dehydrogenase/oxidase, C-terminal	Domain
A, B, C, D, E A, B, C, D, E, F	IPR036250	Acyl-CoA dehydrogenase-like, C-terminal	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR013786	Acyl-CoA dehydrogenase/oxidase, N-terminal	Domain
A, B, C, D, E A, B, C, D, E, F	IPR037069	Acyl-CoA dehydrogenase/oxidase, N-terminal domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.