2BSX

Crystal structure of the Plasmodium falciparum purine nucleoside phosphorylase complexed with inosine

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2bsxa2	Alpha and beta proteins (a/b)	Phosphorylase/hydrolase-like	Purine and uridine phosphorylases	Purine and uridine phosphorylases	automated matches	(Plasmodium falciparum 3D7 ) [TaxId: 36329 ],	SCOPe (2.08)
A	d2bsxa3	Artifacts	Tags	Tags	Tags	C-terminal Tags	(Plasmodium falciparum 3D7 ) [TaxId: 36329 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Purine and uridine phosphorylases	8039540	3000626	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PNP_UDP_1	e2bsxA1	A: a/b three-layered sandwiches	X: Phosphorylase/hydrolase-like	H: Peptidyl-tRNA hydrolase-like (From Topology)	T: Peptidyl-tRNA hydrolase-like	F: PNP_UDP_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.1580	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Nucleoside phosphorylase domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF01048	Phosphorylase superfamily (PNP_UDP_1)	Phosphorylase superfamily	Members of this family include: purine nucleoside phosphorylase (PNP) Uridine phosphorylase (UdRPase) 5'-methylthioadenosine phosphorylase (MTA phosphorylase)	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	PURINE NUCLEOSIDE PHOSPHORYLASE	guanosine phosphorylase activity pentosyltransferase activity glycosyltransferase activity transferase activity catalytic activity purine-nucleoside phosphorylase activity uridine phosphorylase activity pyrimidine-nucleoside phosphorylase activity S-methyl-5-thioadenosine phosphorylase activity	glycosyl compound metabolic process organonitrogen compound biosynthetic process carbohydrate derivative metabolic process nucleoside biosynthetic process nucleobase-containing compound metabolic process organic substance metabolic process nucleobase-containing small molecule metabolic process purine-containing compound biosynthetic process organic substance biosynthetic process small molecule metabolic process cellular metabolic compound salvage purine ribonucleoside metabolic process carbohydrate derivative biosynthetic process organonitrogen compound metabolic process glycosyl compound metabolic process organonitrogen compound biosynthetic process carbohydrate derivative metabolic process nucleoside biosynthetic process nucleobase-containing compound metabolic process organic substance metabolic process nucleobase-containing small molecule metabolic process purine-containing compound biosynthetic process organic substance biosynthetic process small molecule metabolic process cellular metabolic compound salvage purine ribonucleoside metabolic process carbohydrate derivative biosynthetic process organonitrogen compound metabolic process glycosyl compound biosynthetic process purine-containing compound salvage small molecule biosynthetic process nucleoside salvage cellular metabolic process purine ribonucleoside biosynthetic process primary metabolic process ribonucleoside biosynthetic process nucleobase-containing compound biosynthetic process nucleoside metabolic process nucleobase-containing small molecule biosynthetic process purine-containing compound metabolic process purine ribonucleoside salvage organic cyclic compound metabolic process biosynthetic process purine nucleoside biosynthetic process organic cyclic compound biosynthetic process metabolic process cellular process purine nucleoside metabolic process ribonucleoside metabolic process catabolic process purine nucleoside catabolic process inosine catabolic process organonitrogen compound catabolic process carbohydrate derivative catabolic process inosine metabolic process glycosyl compound catabolic process purine-containing compound catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process nucleobase-containing small molecule catabolic process nucleoside catabolic process small molecule catabolic process ribonucleoside catabolic process organic substance catabolic process purine ribonucleoside catabolic process organophosphate catabolic process nucleoside phosphate catabolic process purine nucleotide metabolic process phosphate-containing compound metabolic process phosphorus metabolic process nucleotide catabolic process nucleoside phosphate metabolic process purine nucleotide catabolic process nucleotide metabolic process organophosphate metabolic process pyrimidine-containing compound catabolic process uridine metabolic process pyrimidine nucleoside metabolic process pyrimidine-containing compound metabolic process pyrimidine nucleoside catabolic process uridine catabolic process pyrimidine ribonucleoside metabolic process pyrimidine ribonucleoside catabolic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR035994	Nucleoside phosphorylase superfamily	Homologous Superfamily
A	IPR000845	Nucleoside phosphorylase domain	Domain

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	purine-nucleoside phosphorylase M-CSA #695	Purine metabolism in the parasite Plasmodium has been identified as a promising target for antimalarial therapies due to slight alterations around the active site when compared to mammalian purine nucleoside phosphorylase (PNP). PNP is part of a salvage pathway for the biosynthesis of purines, which are essential to parasite survival due to location of the organism, erthrocytes and Plasmodium itself not being able to produce purines de novo .	Defined by 4 residues: ARG:A-27ARG:A_5-45ARG:A-88ASP:A-206 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 2.4.2.1 (PDB Primary Data) Search M-CSA Motif + EC 2.4.2.1

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.