2BCJ

Crystal Structure of G Protein-Coupled Receptor Kinase 2 in Complex with Galpha-q and Gbetagamma Subunits

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Protein Modification Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2bcja1	All alpha proteins	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	Regulator of G-protein signaling, RGS	G-protein coupled receptor kinase 2, N-terminal domain	Bovine (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
A	d2bcja2	All beta proteins	PH domain-like barrel	PH domain-like	Pleckstrin-homology domain (PH domain)	G-protein coupled receptor kinase 2 (beta-adrenergic receptor kinase 1)	Bovine (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
A	d2bcja3	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	Protein kinases, catalytic subunit	G-protein coupled receptor kinase 2	Bovine (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
B	d2bcjb_	All beta proteins	7-bladed beta-propeller	WD40 repeat-like	WD40-repeat	beta1-subunit of the signal-transducing G protein heterotrimer	Bovine (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
C [auth G]	d2bcjg_	All alpha proteins	Non-globular all-alpha subunits of globular proteins	Transducin (heterotrimeric G protein), gamma chain	Transducin (heterotrimeric G protein), gamma chain	Transducin (heterotrimeric G protein), gamma chain	Bovine (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
D [auth Q]	d2bcjq1	All alpha proteins	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Transducin (alpha subunit), insertion domain	Rat (Rattus norvegicus ) [TaxId: 10116 ], Mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
D [auth Q]	d2bcjq2	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	Transducin (alpha subunit)	Rat (Rattus norvegicus ) [TaxId: 10116 ], Mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Regulator of G-protein signaling RGS	8034089	3000835	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	PH domain-like	8034091	3000134	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Protein kinase-like (PK-like)	8034093	3000066	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	WD40 repeat-like	8042849	3001694	SCOP2B (2022-06-29)
C [auth G]	SCOP2B Superfamily	Transducin (heterotrimeric G protein) gamma chain	8070796	3001771	SCOP2B (2022-06-29)
D [auth Q]	SCOP2B Superfamily	Galpha insert domain-like	8056801	3000669	SCOP2B (2022-06-29)
D [auth Q]	SCOP2B Superfamily	Ras-like P-loop GTPases	8056800	3002022	SCOP2B (2022-06-29)
D [auth Q]	SCOP2B Superfamily	Ras-like P-loop GTPases	8056800	3002022	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PH	e2bcjA1	A: beta barrels	X: PH domain-like	H: PH domain-like (From Topology)	T: PH domain-like	F: PH	ECOD (1.6)
A	RGS	e2bcjA2	A: alpha bundles	X: Regulator of G-protein signaling, RGS (From Topology)	H: Regulator of G-protein signaling, RGS (From Topology)	T: Regulator of G-protein signaling, RGS	F: RGS	ECOD (1.6)
A	Pkinase_Tyr	e2bcjA3	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)
B	ANAPC4_WD40_3	e2bcjB1	A: beta duplicates or obligate multimers	X: beta-propeller-like	H: beta-propeller	T: 7-bladed	F: ANAPC4_WD40_3	ECOD (1.6)
C [auth G]	G-gamma	e2bcjG1	A: extended segments	X: Transducin (heterotrimeric G protein), gamma chain (From Topology)	H: Transducin (heterotrimeric G protein), gamma chain (From Topology)	T: Transducin (heterotrimeric G protein), gamma chain	F: G-gamma	ECOD (1.6)
D [auth Q]	G-alpha	e2bcjQ1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
D [auth Q]	Ras	e2bcjQ2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.287.1270	Mainly Alpha	Orthogonal Bundle	Helix Hairpins		CATH (4.3.0)
A	1.10.167.10	Mainly Alpha	Orthogonal Bundle	Regulator of G-protein Signalling 4	domain 2	CATH (4.3.0)
A	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (4.3.0)
A	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (4.3.0)
A	2.30.29.30	Mainly Beta	Roll	PH-domain like	Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)	CATH (4.3.0)
B	2.130.10.10	Mainly Beta	7 Propeller	Methylamine Dehydrogenase	Chain H	CATH (4.3.0)
C [auth G]	4.10.260.10	Few Secondary Structures	Irregular	G Protein Gi Gamma 2	Transducin (heterotrimeric G protein), gamma chain	CATH (4.3.0)
D [auth Q]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
D [auth Q]	1.10.400.10	Mainly Alpha	Orthogonal Bundle	GI Alpha 1, domain 2-like	GI Alpha 1, domain 2-like	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00615	Regulator of G protein signaling domain (RGS)	Regulator of G protein signaling domain	RGS family members are GTPase-activating proteins for heterotrimeric G-protein alpha-subunits.	Domain
A	PF00069	Protein kinase domain (Pkinase)	Protein kinase domain	-	Domain
A	PF00169	PH domain (PH)	PH domain	PH stands for pleckstrin homology.	Domain
B	PF00400	WD domain, G-beta repeat (WD40)	WD domain, G-beta repeat	-	Repeat
C [auth G]	PF00631	GGL domain (G-gamma)	GGL domain	G-protein gamma like domains (GGL) are found in the gamma subunit of the heterotrimeric G protein complex and in regulators of G protein signaling (RGS) proteins [1]. It is also found fused to an inactive Galpha in the Dictyostelium protein gbqA [2] ...	G-protein gamma like domains (GGL) are found in the gamma subunit of the heterotrimeric G protein complex and in regulators of G protein signaling (RGS) proteins [1]. It is also found fused to an inactive Galpha in the Dictyostelium protein gbqA [2]. G-gamma likely shares a common origin with the helical N-terminal unit of G-beta [2]. All organisms that posses a G-beta possess a G-gamma [2].	Domain
D [auth Q]	PF00503	G-protein alpha subunit (G-alpha)	G-protein alpha subunit	G proteins couple receptors of extracellular signals to intracellular signaling pathways. The G protein alpha subunit binds guanyl nucleotide and is a weak GTPase. A set of residues that are unique to G-alpha as compared to its ancestor the Arf-like ...	G proteins couple receptors of extracellular signals to intracellular signaling pathways. The G protein alpha subunit binds guanyl nucleotide and is a weak GTPase. A set of residues that are unique to G-alpha as compared to its ancestor the Arf-like family form a ring of residues centered on the nucleotide binding site [3]. A Ggamma is found fused to an inactive Galpha in the Dictyostelium protein gbqA [3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	G-protein-coupled receptor kinase 2	catalytic activity, acting on a protein beta-adrenergic receptor kinase activity kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups Edg-2 lysophosphatidic acid receptor binding protein binding binding signaling receptor binding endothelial differentiation G protein-coupled receptor binding G protein-coupled receptor binding catalytic activity, acting on a protein beta-adrenergic receptor kinase activity kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups Edg-2 lysophosphatidic acid receptor binding protein binding binding signaling receptor binding endothelial differentiation G protein-coupled receptor binding G protein-coupled receptor binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding adrenergic receptor binding alpha-2A adrenergic receptor binding protein serine/threonine kinase activity G protein-coupled receptor kinase activity	protein localization regulation of localization regulation of biological process cellular localization regulation of cellular process regulation of protein localization protein localization to cilium biological regulation positive regulation of biological process positive regulation of protein localization to cilium regulation of protein localization to cilium localization regulation of cellular localization cellular macromolecule localization macromolecule localization protein localization regulation of localization regulation of biological process cellular localization regulation of cellular process regulation of protein localization protein localization to cilium biological regulation positive regulation of biological process positive regulation of protein localization to cilium regulation of protein localization to cilium localization regulation of cellular localization cellular macromolecule localization macromolecule localization protein localization to organelle positive regulation of protein localization cellular process positive regulation of cellular process developmental process heart development multicellular organism development multicellular organismal process anatomical structure development animal organ development circulatory system development system development system process regulation of system process negative regulation of biological process muscle contraction muscle system process regulation of muscle system process striated muscle contraction negative regulation of muscle contraction regulation of striated muscle contraction regulation of muscle contraction regulation of multicellular organismal process negative regulation of multicellular organismal process negative regulation of striated muscle contraction heart process regulation of heart contraction circulatory system process regulation of blood circulation regulation of biological quality heart contraction regulation of the force of heart contraction blood circulation cellular response to stimulus regulation of signaling response to stimulus signaling regulation of signal transduction cell communication regulation of response to stimulus regulation of cell communication signal transduction viral genome replication viral process viral life cycle positive regulation of cell communication positive regulation of signal transduction positive regulation of response to stimulus positive regulation of smoothened signaling pathway regulation of smoothened signaling pathway cell surface receptor signaling pathway smoothened signaling pathway positive regulation of signaling negative adaptation of signaling pathway negative regulation of signaling desensitization of G protein-coupled receptor signaling pathway negative regulation of G protein-coupled receptor signaling pathway negative regulation of cell communication negative regulation of cellular process regulation of G protein-coupled receptor signaling pathway negative regulation of response to stimulus negative regulation of signal transduction adaptation of signaling pathway G protein-coupled receptor signaling pathway establishment of protein localization nitrogen compound transport transport organic substance transport intracellular transport establishment of localization protein transport establishment of localization in cell intracellular protein transport negative regulation of blood circulation negative regulation of heart contraction negative regulation of the force of heart contraction by chemical signal regulation of the force of heart contraction by chemical signal protein phosphorylation organonitrogen compound metabolic process phosphorylation cellular metabolic process primary metabolic process organic substance metabolic process macromolecule modification phosphate-containing compound metabolic process metabolic process protein metabolic process macromolecule metabolic process protein modification process phosphorus metabolic process cardiac muscle contraction peptidyl-serine modification peptidyl-serine phosphorylation peptidyl-amino acid modification tachykinin receptor signaling pathway negative regulation of relaxation of smooth muscle relaxation of smooth muscle negative regulation of relaxation of muscle relaxation of muscle regulation of relaxation of smooth muscle regulation of relaxation of muscle biological process involved in interspecies interaction between organisms symbiont entry into host cell symbiont entry into host biological process involved in interaction with host biological process involved in symbiotic interaction cellular response to nitrogen compound response to organic substance synaptic signaling cellular response to acetylcholine response to oxygen-containing compound cell-cell signaling response to nitrogen compound acetylcholine receptor signaling pathway postsynaptic signal transduction cellular response to oxygen-containing compound cellular response to chemical stimulus G protein-coupled acetylcholine receptor signaling pathway response to chemical response to acetylcholine import into cell receptor internalization vesicle-mediated transport G protein-coupled receptor internalization receptor-mediated endocytosis endocytosis	membrane cellular anatomical entity intracellular anatomical structure cytoplasm cytosol cell periphery plasma membrane presynapse synapse cell junction postsynapse cell projection
B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	protein-containing complex binding binding protein binding enzyme binding GTPase binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity signaling receptor complex adaptor activity protein-macromolecule adaptor activity protein-containing complex binding binding protein binding enzyme binding GTPase binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity signaling receptor complex adaptor activity protein-macromolecule adaptor activity molecular adaptor activity signaling adaptor activity	cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation phospholipase C-activating G protein-coupled receptor signaling pathway cell communication G protein-coupled receptor signaling pathway cellular process signal transduction multicellular organismal process system process sensory perception of chemical stimulus cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation phospholipase C-activating G protein-coupled receptor signaling pathway cell communication G protein-coupled receptor signaling pathway cellular process signal transduction multicellular organismal process system process sensory perception of chemical stimulus sensory perception nervous system process sensory perception of taste developmental process sensory organ development eye development animal organ development retina development in camera-type eye visual system development multicellular organism development camera-type eye development anatomical structure development sensory system development system development cell population proliferation cellular response to nitrogen compound response to dopamine response to organic substance dopamine receptor signaling pathway cellular response to dopamine response to monoamine cellular response to endogenous stimulus response to catecholamine adenylate cyclase-activating dopamine receptor signaling pathway cellular response to catecholamine stimulus cellular response to organic substance response to organic cyclic compound response to organonitrogen compound response to endogenous stimulus cellular response to organic cyclic compound response to oxygen-containing compound response to nitrogen compound adenylate cyclase-activating G protein-coupled receptor signaling pathway cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to organonitrogen compound cellular response to monoamine stimulus response to chemical adenylate cyclase-modulating G protein-coupled receptor signaling pathway response to ketone response to prostaglandin response to lipid response to hormone response to prostaglandin E cellular response to ketone cellular response to hormone stimulus response to alcohol cellular response to prostaglandin stimulus cellular response to prostaglandin E stimulus cellular response to alcohol cellular response to lipid	membrane cellular anatomical entity protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex membrane cellular anatomical entity protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane membrane-bounded organelle non-motile cilium 9+0 non-motile cilium cilium neuron projection photoreceptor disc membrane photoreceptor outer segment plasma membrane bounded cell projection cell projection photoreceptor cell cilium organelle organelle membrane intracellular anatomical structure cytoplasm
C [auth G]	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	protein binding binding G-protein beta-subunit binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	cellular response to stimulus cellular response to nitrogen compound response to dopamine response to organic substance regulation of cellular process response to stimulus dopamine receptor signaling pathway signaling cellular response to dopamine response to monoamine cell communication cellular response to endogenous stimulus response to catecholamine adenylate cyclase-activating dopamine receptor signaling pathway cellular response to stimulus cellular response to nitrogen compound response to dopamine response to organic substance regulation of cellular process response to stimulus dopamine receptor signaling pathway signaling cellular response to dopamine response to monoamine cell communication cellular response to endogenous stimulus response to catecholamine adenylate cyclase-activating dopamine receptor signaling pathway cellular response to catecholamine stimulus cellular response to organic substance signal transduction response to organic cyclic compound regulation of biological process response to organonitrogen compound response to endogenous stimulus cellular response to organic cyclic compound biological regulation response to oxygen-containing compound response to nitrogen compound adenylate cyclase-activating G protein-coupled receptor signaling pathway cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to organonitrogen compound cellular response to monoamine stimulus response to chemical adenylate cyclase-modulating G protein-coupled receptor signaling pathway cellular process G protein-coupled receptor signaling pathway response to ketone response to prostaglandin response to lipid response to hormone response to prostaglandin E cellular response to ketone cellular response to hormone stimulus response to alcohol cellular response to prostaglandin stimulus cellular response to prostaglandin E stimulus cellular response to alcohol cellular response to lipid	cell periphery plasma membrane membrane cellular anatomical entity protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane protein complex heterotrimeric G-protein complex side of membrane cytoplasmic side of plasma membrane GTPase complex cell periphery plasma membrane membrane cellular anatomical entity protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane protein complex heterotrimeric G-protein complex side of membrane cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane
D [auth Q]	Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(q) subunit alpha chimera	D2 dopamine receptor binding protein binding binding signaling receptor binding G protein-coupled receptor binding dopamine receptor binding GTPase activating protein binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding D2 dopamine receptor binding protein binding binding signaling receptor binding G protein-coupled receptor binding dopamine receptor binding GTPase activating protein binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding cation binding magnesium ion binding metal ion binding GDP binding G protein-coupled serotonin receptor binding protein-containing complex binding G-protein beta/gamma-subunit complex binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity molecular function activator activity nucleoside-triphosphatase regulator activity molecular function regulator activity phosphoric ester hydrolase activity alkylglycerophosphoethanolamine phosphodiesterase activity hydrolase activity, acting on ester bonds phosphoric diester hydrolase activity G protein activity	cellular process cell division cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway signal transduction cell cycle regulation of cytoskeleton organization cellular process cell division cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway signal transduction cell cycle regulation of cytoskeleton organization regulation of cellular component organization microtubule cytoskeleton organization regulation of mitotic spindle organization mitotic cell cycle cellular component organization cellular component organization or biogenesis regulation of cell cycle microtubule cytoskeleton organization involved in mitosis spindle organization cell cycle process regulation of cell cycle process regulation of microtubule-based process regulation of spindle organization regulation of organelle organization mitotic spindle organization organelle organization mitotic cell cycle process microtubule-based process cytoskeleton organization regulation of microtubule cytoskeleton organization protein localization positive regulation of protein localization to cell periphery regulation of localization protein localization to cell periphery cellular localization regulation of protein localization positive regulation of biological process positive regulation of protein localization to cell cortex regulation of protein localization to cell periphery localization regulation of cellular localization regulation of protein localization to cell cortex cellular macromolecule localization macromolecule localization positive regulation of protein localization protein localization to cell cortex positive regulation of cellular process trans-synaptic signaling regulation of signaling negative regulation of signaling modulation of chemical synaptic transmission negative regulation of biological process cell-cell signaling negative regulation of synaptic transmission negative regulation of cell communication chemical synaptic transmission synaptic signaling regulation of trans-synaptic signaling anterograde trans-synaptic signaling negative regulation of cellular process regulation of cell communication regulation of signal transduction intracellular signal transduction cAMP-mediated signaling regulation of intracellular signal transduction regulation of response to stimulus intracellular signaling cassette regulation of cAMP-mediated signaling response to organic substance response to ketone response to lipid response to alcohol cellular response to forskolin cellular response to organic cyclic compound response to oxygen-containing compound cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to alcohol response to chemical response to forskolin cellular response to lipid cellular response to organic substance cellular response to ketone response to organic cyclic compound cell surface receptor signaling pathway regulation of Wnt signaling pathway regulation of canonical Wnt signaling pathway canonical Wnt signaling pathway Wnt signaling pathway developmental process heart development multicellular organism development multicellular organismal process anatomical structure development animal organ development circulatory system development system development system process circulatory system process regulation of blood pressure regulation of biological quality blood circulation regulation of protein stability protein stabilization cellular developmental process cell development developmental maturation nervous system development generation of neurons neurogenesis neuron differentiation neuron remodeling anatomical structure maturation cell differentiation neuron development neuron maturation cell maturation skeletal system development glutamate receptor signaling pathway pigmentation developmental pigmentation ion channel modulating, G protein-coupled receptor signaling pathway phospholipase C-activating G protein-coupled receptor signaling pathway regulation of body fluid levels wound healing cell activation hemostasis regulation of cell activation blood coagulation regulation of platelet activation response to stress coagulation regulation of multicellular organismal process response to wounding platelet activation cell population proliferation regulation of smooth muscle cell proliferation positive regulation of cell population proliferation smooth muscle cell proliferation positive regulation of smooth muscle cell proliferation regulation of cell population proliferation muscle cell proliferation forebrain development central nervous system neuron development central nervous system neuron differentiation head development forebrain generation of neurons brain development forebrain neuron development central nervous system development forebrain neuron differentiation phospholipase C-activating dopamine receptor signaling pathway cellular response to nitrogen compound response to dopamine dopamine receptor signaling pathway cellular response to dopamine response to monoamine cellular response to endogenous stimulus response to catecholamine cellular response to catecholamine stimulus response to organonitrogen compound response to endogenous stimulus response to nitrogen compound cellular response to organonitrogen compound cellular response to monoamine stimulus negative regulation of potassium ion transport regulation of potassium ion transport transport regulation of transport negative regulation of monoatomic ion transport regulation of monoatomic ion transport negative regulation of transport establishment of localization metal ion transport monoatomic ion transport potassium ion transport monoatomic cation transport regulation of metal ion transport phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway cellular response to acetylcholine acetylcholine receptor signaling pathway postsynaptic signal transduction G protein-coupled acetylcholine receptor signaling pathway response to acetylcholine cranial skeletal system development negative regulation of programmed cell death regulation of apoptotic process regulation of programmed cell death cell death negative regulation of apoptotic process apoptotic process programmed cell death action potential regulation of membrane potential maternal behavior behavior parental behavior reproductive process multicellular organismal reproductive process reproductive behavior pigment cell differentiation regulation of developmental process regulation of pigment cell differentiation regulation of cell differentiation melanocyte differentiation regulation of melanocyte differentiation adenylate cyclase-activating G protein-coupled receptor signaling pathway endothelin receptor signaling pathway positive regulation of cell communication establishment of protein localization nitrogen compound transport positive regulation of establishment of protein localization positive regulation of insulin secretion regulation of peptide hormone secretion regulation of hormone levels organic substance transport positive regulation of peptide hormone secretion establishment of protein localization to extracellular region protein secretion regulation of protein secretion regulation of secretion regulation of hormone secretion regulation of peptide secretion positive regulation of secretion by cell hormone secretion protein localization to extracellular region positive regulation of protein transport peptide secretion secretion by cell regulation of secretion by cell positive regulation of hormone secretion positive regulation of secretion amide transport signal release positive regulation of peptide secretion secretion regulation of peptide transport regulation of establishment of protein localization export from cell regulation of protein transport positive regulation of protein secretion peptide transport positive regulation of transport protein transport regulation of insulin secretion peptide hormone secretion hormone transport insulin secretion positive regulation of signaling post-embryonic development ligand-gated ion channel signaling pathway limb development appendage development embryonic limb morphogenesis embryonic morphogenesis embryonic appendage morphogenesis embryo development anatomical structure morphogenesis appendage morphogenesis embryonic digit morphogenesis limb morphogenesis	midbody cellular anatomical entity cell periphery cell cortex intracellular anatomical structure cytoplasm protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex midbody cellular anatomical entity cell periphery cell cortex intracellular anatomical structure cytoplasm protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane intracellular organelle centrosome microtubule organizing center cytoskeleton organelle intracellular non-membrane-bounded organelle microtubule cytoskeleton non-membrane-bounded organelle membrane-bounded organelle nucleus intracellular membrane-bounded organelle nuclear membrane endomembrane system organelle envelope nuclear envelope organelle membrane somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection Golgi apparatus cell body membrane microdomain membrane raft plasma membrane raft plasma membrane region caveola synapse cell junction

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR016137	RGS domain	Domain
A	IPR017441	Protein kinase, ATP binding site	Binding Site
A	IPR001849	Pleckstrin homology domain	Domain
A	IPR036305	RGS domain superfamily	Homologous Superfamily
A	IPR000719	Protein kinase domain	Domain
A	IPR011993	PH-like domain superfamily	Homologous Superfamily
A	IPR000961	AGC-kinase, C-terminal	Domain
A	IPR044926	RGS, subdomain 2	Homologous Superfamily
A	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A	IPR000239	GPCR kinase	Family
A	IPR008271	Serine/threonine-protein kinase, active site	Active Site
B	IPR019775	WD40 repeat, conserved site	Conserved Site
B	IPR016346	Guanine nucleotide-binding protein, beta subunit	Family
B	IPR036322	WD40-repeat-containing domain superfamily	Homologous Superfamily
B	IPR001632	G-protein, beta subunit	Domain
B	IPR001680	WD40 repeat	Repeat
B	IPR020472	G-protein beta WD-40 repeat	Repeat
B	IPR015943	WD40/YVTN repeat-like-containing domain superfamily	Homologous Superfamily
C [auth G]	IPR036284	G-protein gamma-like domain superfamily	Homologous Superfamily
C [auth G]	IPR015898	G-protein gamma-like domain	Domain
C [auth G]	IPR001770	G-protein, gamma subunit	Family
D [auth Q]	IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	Family
D [auth Q]	IPR011025	G protein alpha subunit, helical insertion	Homologous Superfamily
D [auth Q]	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
D [auth Q]	IPR001408	G-protein alpha subunit, group I	Family
D [auth Q]	IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	Family
D [auth Q]	IPR000654	G-protein alpha subunit, group Q	Family
D [auth Q]	IPR011025	G protein alpha subunit, helical insertion	Homologous Superfamily
D [auth Q]	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, C [auth G], D [auth Q]	OPM	Monotopic/peripheral	All beta monotopic/peripheral	PH domain-like	Pleckstrin-homology domain

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
B	ACE	RESID: AA0041 , AA0042 , AA0043 , AA0044 , AA0045 , AA0046 , AA0049 , AA0050 , AA0051 , AA0052 , AA0053 , AA0054 , AA0354 PSI-MOD : N-acetyl-L-alanine MOD:00050 , N-acetyl-L-aspartic acid MOD:00051 , N-acetyl-L-cysteine MOD:00052 , N-acetyl-S-archeol-cysteine MOD:00897 , N-acetyl-L-glutamic acid MOD:00053 , N-acetyl-L-glutamine MOD:00054 , N-acetylglycine MOD:00055 , N-acetyl-L-methionine MOD:00058 , N-acetyl-L-proline MOD:00059 , N-acetyl-L-serine MOD:00060 , N,O-diacetylated L-serine MOD:00648 , N-acetyl-L-threonine MOD:00061 , N-acetyl-L-tyrosine MOD:00062 , N-acetyl-L-valine MOD:00063 , N2-acetyl-L-arginine MOD:00359