2B26

The crystal structure of the protein complex of yeast Hsp40 Sis1 and Hsp70 Ssa1

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2b26a1	All beta proteins	HSP40/DnaJ peptide-binding domain	HSP40/DnaJ peptide-binding domain	HSP40/DnaJ peptide-binding domain	Heat shock protein 40 Sis1	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
A	d2b26a3	Artifacts	Tags	Tags	Tags	N-terminal Tags	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
B	d2b26b1	All beta proteins	HSP40/DnaJ peptide-binding domain	HSP40/DnaJ peptide-binding domain	HSP40/DnaJ peptide-binding domain	Heat shock protein 40 Sis1	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
B	d2b26b3	Artifacts	Tags	Tags	Tags	N-terminal Tags	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	hsp40/dnaj peptide-binding domain	8040504	3000885	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	hsp40/dnaj peptide-binding domain	8040507	3000885	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	hsp40/dnaj peptide-binding domain	8040507	3000885	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	hsp40/dnaj peptide-binding domain	8040507	3000885	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	DnaJ_C_C	e2b26A4	A: beta sandwiches	X: HSP40/DnaJ peptide-binding domain (From Topology)	H: HSP40/DnaJ peptide-binding domain (From Topology)	T: HSP40/DnaJ peptide-binding domain	F: DnaJ_C_C	ECOD (1.6)
A	DnaJ_C_N_1	e2b26A3	A: beta sandwiches	X: HSP40/DnaJ peptide-binding domain (From Topology)	H: HSP40/DnaJ peptide-binding domain (From Topology)	T: HSP40/DnaJ peptide-binding domain	F: DnaJ_C_N_1	ECOD (1.6)
B	DnaJ_C_C	e2b26B4	A: beta sandwiches	X: HSP40/DnaJ peptide-binding domain (From Topology)	H: HSP40/DnaJ peptide-binding domain (From Topology)	T: HSP40/DnaJ peptide-binding domain	F: DnaJ_C_C	ECOD (1.6)
B	DnaJ_C_N_1	e2b26B3	A: beta sandwiches	X: HSP40/DnaJ peptide-binding domain (From Topology)	H: HSP40/DnaJ peptide-binding domain (From Topology)	T: HSP40/DnaJ peptide-binding domain	F: DnaJ_C_N_1	ECOD (1.6)
C	DnaJ_C_C	e2b26C1	A: beta sandwiches	X: HSP40/DnaJ peptide-binding domain (From Topology)	H: HSP40/DnaJ peptide-binding domain (From Topology)	T: HSP40/DnaJ peptide-binding domain	F: DnaJ_C_C	ECOD (1.6)
C	DnaJ_C_N_1	e2b26C2	A: beta sandwiches	X: HSP40/DnaJ peptide-binding domain (From Topology)	H: HSP40/DnaJ peptide-binding domain (From Topology)	T: HSP40/DnaJ peptide-binding domain	F: DnaJ_C_N_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.260.20	Mainly Beta	Sandwich	HSP40/DNAj peptide-binding domain	Urease metallochaperone UreE, N-terminal domain	CATH (4.3.0)
B	2.60.260.20	Mainly Beta	Sandwich	HSP40/DNAj peptide-binding domain	Urease metallochaperone UreE, N-terminal domain	CATH (4.3.0)
C	2.60.260.20	Mainly Beta	Sandwich	HSP40/DNAj peptide-binding domain	Urease metallochaperone UreE, N-terminal domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C	PF01556	DnaJ C terminal domain (DnaJ_C)	DnaJ C terminal domain	This family consists of the C terminal region of the DnaJ protein. It is always found associated with Pfam:PF00226 and Pfam:PF00684. DnaJ is a chaperone associated with the Hsp70 heat-shock system involved in protein folding and renaturation after ...	This family consists of the C terminal region of the DnaJ protein. It is always found associated with Pfam:PF00226 and Pfam:PF00684. DnaJ is a chaperone associated with the Hsp70 heat-shock system involved in protein folding and renaturation after stress. The two C-terminal domains CTDI and CTDII, both incorporated in this family are necessary for maintaining the J-domains in their specific relative positions [2]. Structural analysis of PDB:1nlt shows that PF00684 is nested within this DnaJ C-terminal region [3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C	SIS1 protein	DNA binding binding organic cyclic compound binding nucleic acid binding unfolded protein binding protein binding misfolded protein binding protein-folding chaperone binding	cellular process cell cycle protein localization cellular localization establishment of protein localization nitrogen compound transport transport misfolded protein transport organic substance transport intracellular transport establishment of localization localization protein transport establishment of localization in cell cellular process cell cycle protein localization cellular localization establishment of protein localization nitrogen compound transport transport misfolded protein transport organic substance transport intracellular transport establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization intracellular protein transport cellular response to stimulus response to organic substance catabolic process response to stimulus proteolysis involved in protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process macromolecule catabolic process cellular response to misfolded protein response to misfolded protein organic substance metabolic process cellular response to stress modification-dependent macromolecule catabolic process proteolysis response to stress protein metabolic process cellular response to organic substance organonitrogen compound catabolic process organonitrogen compound metabolic process cellular response to topologically incorrect protein primary metabolic process proteasomal protein catabolic process protein catabolic process nuclear protein quality control by the ubiquitin-proteasome system cellular response to chemical stimulus modification-dependent protein catabolic process response to chemical response to topologically incorrect protein ubiquitin-dependent protein catabolic process metabolic process macromolecule metabolic process protein quality control for misfolded or incompletely synthesized proteins organic substance catabolic process cellular biosynthetic process cellular metabolic process organonitrogen compound biosynthetic process translation gene expression macromolecule biosynthetic process biosynthetic process organic substance biosynthetic process translational initiation mitochondria-associated ubiquitin-dependent protein catabolic process protein maturation protein folding chaperone cofactor-dependent protein refolding chaperone-mediated protein folding 'de novo' protein folding 'de novo' post-translational protein folding RNA import into nucleus RNA transport nucleocytoplasmic transport nuclear transport nucleic acid transport tRNA import into nucleus import into nucleus tRNA transport establishment of RNA localization RNA localization nucleobase-containing compound transport	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol cytosolic small ribosomal subunit protein-containing complex small ribosomal subunit intracellular organelle ribosomal subunit cytosolic ribosome organelle ribonucleoprotein complex intracellular non-membrane-bounded organelle ribosome intracellular anatomical structure cytoplasm cellular anatomical entity cytosol cytosolic small ribosomal subunit protein-containing complex small ribosomal subunit intracellular organelle ribosomal subunit cytosolic ribosome organelle ribonucleoprotein complex intracellular non-membrane-bounded organelle ribosome non-membrane-bounded organelle membrane-bounded organelle nucleus intracellular membrane-bounded organelle
D	Heat shock 70 kDa protein cognate 2	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity ATP-dependent activity protein binding heat shock protein binding protein folding chaperone ATP-dependent protein folding chaperone	protein refolding organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process protein folding biosynthetic process organic substance biosynthetic process metabolic process cellular process protein refolding organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process protein folding biosynthetic process organic substance biosynthetic process metabolic process cellular process protein metabolic process macromolecule metabolic process chaperone cofactor-dependent protein refolding chaperone-mediated protein folding 'de novo' protein folding 'de novo' post-translational protein folding	intracellular anatomical structure cytoplasm cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C	IPR018253	DnaJ domain, conserved site	Conserved Site
A, B, C	IPR002939	Chaperone DnaJ, C-terminal	Domain
A, B, C	IPR036869	Chaperone J-domain superfamily	Homologous Superfamily
A, B, C	IPR001623	DnaJ domain	Domain
A, B, C	IPR008971	HSP40/DnaJ peptide-binding	Homologous Superfamily
D	IPR029048	Heat shock protein 70kD, C-terminal domain superfamily	Homologous Superfamily
D	IPR018181	Heat shock protein 70, conserved site	Conserved Site
D	IPR029047	Heat shock protein 70kD, peptide-binding domain superfamily	Homologous Superfamily
D	IPR043129	ATPase, nucleotide binding domain	Homologous Superfamily
D	IPR013126	Heat shock protein 70 family	Family

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.