2AW5

Crystal structure of a human malic enzyme

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2aw5a2	Alpha and beta proteins (a/b)	NAD(P)-binding Rossmann-fold domains	NAD(P)-binding Rossmann-fold domains	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d2aw5a1	Alpha and beta proteins (a/b)	Aminoacid dehydrogenase-like, N-terminal domain	Aminoacid dehydrogenase-like, N-terminal domain	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2aw5b2	Alpha and beta proteins (a/b)	NAD(P)-binding Rossmann-fold domains	NAD(P)-binding Rossmann-fold domains	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2aw5b1	Alpha and beta proteins (a/b)	Aminoacid dehydrogenase-like, N-terminal domain	Aminoacid dehydrogenase-like, N-terminal domain	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d2aw5c2	Alpha and beta proteins (a/b)	NAD(P)-binding Rossmann-fold domains	NAD(P)-binding Rossmann-fold domains	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d2aw5c1	Alpha and beta proteins (a/b)	Aminoacid dehydrogenase-like, N-terminal domain	Aminoacid dehydrogenase-like, N-terminal domain	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF03949	e2aw5A3	A: alpha arrays	X: C-terminal domain of Malic enzyme (From Topology)	H: C-terminal domain of Malic enzyme (From Topology)	T: C-terminal domain of Malic enzyme	F: PF03949	ECOD (1.6)
A	PF03949	e2aw5A2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: PF03949	ECOD (1.6)
A	PF00390	e2aw5A1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Aminoacid dehydrogenase-like, N-terminal domain	F: PF00390	ECOD (1.6)
B	PF03949	e2aw5B3	A: alpha arrays	X: C-terminal domain of Malic enzyme (From Topology)	H: C-terminal domain of Malic enzyme (From Topology)	T: C-terminal domain of Malic enzyme	F: PF03949	ECOD (1.6)
B	PF03949	e2aw5B2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: PF03949	ECOD (1.6)
B	PF00390	e2aw5B1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Aminoacid dehydrogenase-like, N-terminal domain	F: PF00390	ECOD (1.6)
C	PF03949	e2aw5C3	A: alpha arrays	X: C-terminal domain of Malic enzyme (From Topology)	H: C-terminal domain of Malic enzyme (From Topology)	T: C-terminal domain of Malic enzyme	F: PF03949	ECOD (1.6)
C	PF03949	e2aw5C2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: NAD(P)-binding Rossmann-fold domains	F: PF03949	ECOD (1.6)
C	PF00390	e2aw5C1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Aminoacid dehydrogenase-like, N-terminal domain	F: PF00390	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.10380	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Malic enzyme, N-terminal domain	CATH (4.3.0)
A	3.40.50.720	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	NAD(P)-binding Rossmann-like Domain	CATH (4.3.0)
B	3.40.50.10380	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Malic enzyme, N-terminal domain	CATH (4.3.0)
B	3.40.50.720	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	NAD(P)-binding Rossmann-like Domain	CATH (4.3.0)
C	3.40.50.10380	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Malic enzyme, N-terminal domain	CATH (4.3.0)
C	3.40.50.720	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	NAD(P)-binding Rossmann-like Domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C	PF00390	Malic enzyme, N-terminal domain (malic)	Malic enzyme, N-terminal domain	-	Domain
A, B, C	PF03949	Malic enzyme, NAD binding domain (Malic_M)	Malic enzyme, NAD binding domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C	NADP-dependent malic enzyme	oxidoreductase activity malate dehydrogenase (decarboxylating) (NADP+) activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors malic enzyme activity malate dehydrogenase activity catalytic activity cation binding manganese ion binding transition metal ion binding ion binding binding metal ion binding small molecule binding oxidoreductase activity malate dehydrogenase (decarboxylating) (NADP+) activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on CH-OH group of donors malic enzyme activity malate dehydrogenase activity catalytic activity cation binding manganese ion binding transition metal ion binding ion binding binding metal ion binding small molecule binding oxaloacetate decarboxylase activity carboxy-lyase activity lyase activity carbon-carbon lyase activity electron transfer activity magnesium ion binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding nucleoside phosphate binding ADP binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding NADP binding NAD binding identical protein binding protein binding	carbohydrate metabolic process organic substance metabolic process primary metabolic process metabolic process oxoacid metabolic process malate metabolic process cellular metabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process small molecule metabolic process cellular process organic acid metabolic process protein-containing complex organization protein homotetramerization carbohydrate metabolic process organic substance metabolic process primary metabolic process metabolic process oxoacid metabolic process malate metabolic process cellular metabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process small molecule metabolic process cellular process organic acid metabolic process protein-containing complex organization protein homotetramerization cellular component organization cellular component biogenesis cellular component assembly protein complex oligomerization protein tetramerization protein-containing complex assembly protein homooligomerization cellular component organization or biogenesis pyruvate metabolic process monocarboxylic acid metabolic process nucleoside phosphate metabolic process nicotinamide nucleotide metabolic process organonitrogen compound metabolic process nucleotide metabolic process purine-containing compound metabolic process NADP metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process purine nucleotide metabolic process organophosphate metabolic process nucleobase-containing small molecule metabolic process phosphate-containing compound metabolic process pyridine-containing compound metabolic process phosphorus metabolic process pyridine nucleotide metabolic process nucleotide biosynthetic process nucleobase-containing compound biosynthetic process biosynthetic process organophosphate biosynthetic process organic cyclic compound biosynthetic process nucleoside phosphate biosynthetic process organic substance biosynthetic process NAD metabolic process NADH metabolic process regulation of cellular process regulation of primary metabolic process regulation of small molecule metabolic process regulation of nucleotide metabolic process regulation of phosphate metabolic process regulation of NADP metabolic process regulation of nucleobase-containing compound metabolic process regulation of biological process biological regulation regulation of cellular metabolic process regulation of purine nucleotide metabolic process regulation of phosphorus metabolic process regulation of metabolic process response to organic substance response to carbohydrate response to stimulus response to chemical response to oxygen-containing compound response to hormone response to endogenous stimulus	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle cytosol

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C	IPR012302	Malic enzyme, NAD-binding	Domain
A, B, C	IPR046346	Aminoacid dehydrogenase-like, N-terminal domain superfamily	Homologous Superfamily
A, B, C	IPR015884	Malic enzyme, conserved site	Conserved Site
A, B, C	IPR012301	Malic enzyme, N-terminal domain	Domain
A, B, C	IPR036291	NAD(P)-binding domain superfamily	Homologous Superfamily
A, B, C	IPR037062	Malic enzyme, N-terminal domain superfamily	Homologous Superfamily
A, B, C	IPR001891	Malic oxidoreductase	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C	Pharos: P48163	obesity disorder Weight Gain medulloblastoma ovarian cancer primitive neuroectodermal tumor amyotrophic lateral sclerosis tuberculosis atypical teratoid rhabdoid tumor breast carcinoma drug-induced liver injury hepatocellular carcinoma lung cancer malignant glioma myopathy oligodendroglioma obesity disorder Weight Gain medulloblastoma ovarian cancer primitive neuroectodermal tumor amyotrophic lateral sclerosis tuberculosis atypical teratoid rhabdoid tumor breast carcinoma drug-induced liver injury hepatocellular carcinoma lung cancer malignant glioma myopathy oligodendroglioma pancreatic intraductal papillary-mucinous carcinoma psoriasis astrocytic tumor ulcerative colitis bipolar disorder breast fibroadenoma ductal breast carcinoma in situ large cell medulloblastoma malignant colon neoplasm MALT lymphoma nasopharyngeal carcinoma osteosarcoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia rheumatoid arthritis atopic eczema breast cancer Crohn disease glioblastoma malignant ependymoma

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.