2ASS

Crystal structure of the Skp1-Skp2-Cks1 complex

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2assa2	All alpha proteins	Skp1 dimerisation domain-like	Skp1 dimerisation domain-like	Skp1 dimerisation domain-like	Cyclin A/CDK2-associated p45, Skp1	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
A	d2assa1	Alpha and beta proteins (a+b)	POZ domain	POZ domain	BTB/POZ domain	Cyclin A/CDK2-associated p45, Skp1	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2assb1	All alpha proteins	F-box domain	F-box domain	F-box domain	Skp2	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
B	d2assb2	Alpha and beta proteins (a/b)	Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)	RNI-like	Cyclin A/CDK2-associated p19, Skp2	Cyclin A/CDK2-associated p19, Skp2	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
C	d2assc_	Alpha and beta proteins (a+b)	Cell cycle regulatory proteins	Cell cycle regulatory proteins	Cell cycle regulatory proteins	CksHs1	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Skp1 dimerisation domain-like	8038683	3000564	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	POZ domain	8035033	3000894	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	F-box domain	8038678	3000567	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	RNI-like	8037339	3000990	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Cell cycle regulatory proteins	8037340	3000311	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Skp1_POZ	e2assA1	A: a+b two layers	X: POZ domain (From Topology)	H: POZ domain (From Topology)	T: POZ domain	F: Skp1_POZ	ECOD (1.6)
B	LRR_8_4	e2assB2	A: beta duplicates or obligate multimers	X: Single-stranded right-handed beta-helix	H: Leucine-rich repeats (From Topology)	T: Leucine-rich repeats	F: LRR_8_4	ECOD (1.6)
B	F-box-like	e2assB1	A: alpha arrays	X: F-box domain (From Topology)	H: F-box domain (From Topology)	T: F-box domain	F: F-box-like	ECOD (1.6)
C	CKS	e2assC1	A: a+b complex topology	X: Cell cycle regulatory proteins (From Topology)	H: Cell cycle regulatory proteins (From Topology)	T: Cell cycle regulatory proteins	F: CKS	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.710.10	Alpha Beta	2-Layer Sandwich	Potassium Channel Kv1.1	Chain A	CATH (4.3.0)
B	3.80.10.10	Alpha Beta	Alpha-Beta Horseshoe	Leucine-rich repeat, LRR (right-handed beta-alpha superhelix)	Ribonuclease Inhibitor	CATH (4.3.0)
C	3.30.170.10	Alpha Beta	2-Layer Sandwich	Cyclin-Dependent Kinase Subunit Type 2	Cyclin-dependent kinase, regulatory subunit	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF01466	Skp1 family, dimerisation domain (Skp1)	Skp1 family, dimerisation domain	-	Domain
A	PF03931	Skp1 family, tetramerisation domain (Skp1_POZ)	Skp1 family, tetramerisation domain	-	Domain
B	PF12937	F-box-like (F-box-like)	F-box-like	This is an F-box-like family.	Domain
C	PF01111	Cyclin-dependent kinase regulatory subunit (CKS)	Cyclin-dependent kinase regulatory subunit	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	S-phase kinase-associated protein 1A	ubiquitin ligase complex scaffold activity protein-macromolecule adaptor activity molecular adaptor activity protein binding binding protein domain specific binding cullin family protein binding ubiquitin-like ligase-substrate adaptor activity enzyme-substrate adaptor activity molecular function activator activity molecular function regulator activity beta-catenin binding F-box domain binding	maintenance of protein location protein localization cellular localization maintenance of protein location in nucleus protein localization to nucleus maintenance of location maintenance of location in cell localization maintenance of protein location in cell cellular macromolecule localization macromolecule localization protein localization to organelle cellular process maintenance of protein localization in organelle maintenance of protein location protein localization cellular localization maintenance of protein location in nucleus protein localization to nucleus maintenance of location maintenance of location in cell localization maintenance of protein location in cell cellular macromolecule localization macromolecule localization protein localization to organelle cellular process maintenance of protein localization in organelle organonitrogen compound metabolic process primary metabolic process organic substance metabolic process protein ubiquitination macromolecule modification metabolic process protein metabolic process macromolecule metabolic process post-translational protein modification protein modification process protein modification by small protein conjugation protein modification by small protein conjugation or removal organonitrogen compound catabolic process catabolic process proteolysis involved in protein catabolic process proteasomal protein catabolic process protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process macromolecule catabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis SCF-dependent proteasomal ubiquitin-dependent protein catabolic process ubiquitin-dependent protein catabolic process organic substance catabolic process regulation of protein modification process regulation of catalytic activity positive regulation of protein metabolic process positive regulation of biological process regulation of protein ubiquitination regulation of transferase activity positive regulation of post-translational protein modification regulation of primary metabolic process regulation of ubiquitin protein ligase activity positive regulation of metabolic process positive regulation of molecular function positive regulation of ubiquitin protein ligase activity positive regulation of protein modification process positive regulation of macromolecule metabolic process regulation of ubiquitin-protein transferase activity regulation of protein modification by small protein conjugation or removal positive regulation of protein modification by small protein conjugation or removal positive regulation of protein ubiquitination regulation of biological process positive regulation of transferase activity regulation of post-translational protein modification regulation of protein metabolic process biological regulation positive regulation of catalytic activity regulation of macromolecule metabolic process positive regulation of ubiquitin-protein transferase activity regulation of molecular function regulation of metabolic process protein polyubiquitination protein K48-linked ubiquitination protein monoubiquitination protein-DNA complex organization cellular component organization protein-containing complex organization chromatin organization chromatin remodeling cellular component organization or biogenesis	membrane-bounded organelle intracellular organelle protein-containing complex intracellular anatomical structure organelle nucleus nuclear protein-containing complex intracellular membrane-bounded organelle cellular anatomical entity PcG protein complex centrosome microtubule organizing center cytoskeleton intracellular non-membrane-bounded organelle membrane-bounded organelle intracellular organelle protein-containing complex intracellular anatomical structure organelle nucleus nuclear protein-containing complex intracellular membrane-bounded organelle cellular anatomical entity PcG protein complex centrosome microtubule organizing center cytoskeleton intracellular non-membrane-bounded organelle microtubule cytoskeleton non-membrane-bounded organelle cytoplasm cytosol membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen cullin-RING ubiquitin ligase complex ubiquitin ligase complex catalytic complex SCF ubiquitin ligase complex transferase complex intracellular protein-containing complex Cul7-RING ubiquitin ligase complex
B	S-phase kinase-associated protein 2	identical protein binding protein binding binding	organonitrogen compound metabolic process protein polyubiquitination primary metabolic process organic substance metabolic process protein ubiquitination macromolecule modification metabolic process protein metabolic process macromolecule metabolic process post-translational protein modification protein modification process protein K48-linked ubiquitination protein modification by small protein conjugation protein modification by small protein conjugation or removal organonitrogen compound metabolic process protein polyubiquitination primary metabolic process organic substance metabolic process protein ubiquitination macromolecule modification metabolic process protein metabolic process macromolecule metabolic process post-translational protein modification protein modification process protein K48-linked ubiquitination protein modification by small protein conjugation protein modification by small protein conjugation or removal mitotic cell cycle process cellular process cell cycle phase transition cell cycle G1/S transition of mitotic cell cycle cell cycle G1/S phase transition mitotic cell cycle mitotic cell cycle phase transition cell cycle process biological process involved in interspecies interaction between organisms response to stimulus immune response response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus immune system process defense response to other organism response to stress innate immune response response to external biotic stimulus regulation of biological process regulation of cellular process regulation of apoptotic process biological regulation apoptotic process regulation of programmed cell death cell death programmed cell death regulation of cell cycle defense response to virus response to virus cell cycle G2/M phase transition G2/M transition of mitotic cell cycle cellular response to stimulus positive regulation of cell communication response to organic substance positive regulation of signal transduction intracellular receptor signaling pathway response to lipid signaling positive regulation of biological process positive regulation of intracellular estrogen receptor signaling pathway regulation of signal transduction intracellular estrogen receptor signaling pathway hormone-mediated signaling pathway cell communication positive regulation of response to stimulus cellular response to endogenous stimulus response to hormone response to steroid hormone regulation of intracellular estrogen receptor signaling pathway regulation of intracellular steroid hormone receptor signaling pathway signal transduction cellular response to organic substance cellular response to steroid hormone stimulus response to organic cyclic compound positive regulation of cellular process cellular response to hormone stimulus regulation of signaling response to endogenous stimulus cellular response to organic cyclic compound positive regulation of intracellular steroid hormone receptor signaling pathway cellular response to chemical stimulus steroid hormone mediated signaling pathway regulation of response to stimulus response to chemical cellular response to lipid nuclear receptor signaling pathway regulation of cell communication intracellular steroid hormone receptor signaling pathway positive regulation of signaling organonitrogen compound catabolic process catabolic process proteolysis involved in protein catabolic process proteasomal protein catabolic process protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process macromolecule catabolic process modification-dependent macromolecule catabolic process modification-dependent protein catabolic process proteolysis SCF-dependent proteasomal ubiquitin-dependent protein catabolic process ubiquitin-dependent protein catabolic process organic substance catabolic process regulation of protein modification process positive regulation of protein metabolic process regulation of protein ubiquitination positive regulation of post-translational protein modification regulation of primary metabolic process positive regulation of metabolic process positive regulation of protein modification process positive regulation of macromolecule metabolic process regulation of protein modification by small protein conjugation or removal positive regulation of protein modification by small protein conjugation or removal positive regulation of protein ubiquitination regulation of protein polyubiquitination regulation of post-translational protein modification regulation of protein metabolic process regulation of macromolecule metabolic process positive regulation of protein polyubiquitination regulation of metabolic process	membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle cellular anatomical entity nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle intracellular non-membrane-bounded organelle nuclear lumen non-membrane-bounded organelle membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle cellular anatomical entity nucleolus intracellular organelle lumen intracellular organelle organelle lumen organelle intracellular non-membrane-bounded organelle nuclear lumen non-membrane-bounded organelle cytoplasm cytosol nucleoplasm cullin-RING ubiquitin ligase complex protein-containing complex ubiquitin ligase complex catalytic complex SCF ubiquitin ligase complex transferase complex intracellular protein-containing complex
C	Cyclin-dependent kinases regulatory subunit 1	protein binding binding histone binding kinase binding enzyme binding protein kinase binding ubiquitin binding ubiquitin-like protein binding cyclin-dependent protein serine/threonine kinase activator activity enzyme regulator activity protein serine/threonine kinase activator activity molecular function activator activity protein kinase regulator activity kinase activator activity protein binding binding histone binding kinase binding enzyme binding protein kinase binding ubiquitin binding ubiquitin-like protein binding cyclin-dependent protein serine/threonine kinase activator activity enzyme regulator activity protein serine/threonine kinase activator activity molecular function activator activity protein kinase regulator activity kinase activator activity enzyme activator activity cyclin-dependent protein serine/threonine kinase regulator activity molecular function regulator activity protein kinase activator activity kinase regulator activity	regulation of cellular process cellular biosynthetic process RNA metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process organic substance metabolic process organic substance biosynthetic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of cellular process cellular biosynthetic process RNA metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process DNA-templated transcription regulation of RNA metabolic process organic substance metabolic process organic substance biosynthetic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of biological process cellular metabolic process biological regulation primary metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process cellular process regulation of DNA-templated transcription macromolecule metabolic process regulation of metabolic process fibroblast proliferation cell population proliferation regulation of mitotic cell cycle cell cycle regulation of cell cycle mitotic cell cycle cell division mitotic cell cycle process cell cycle phase transition mitotic cell cycle phase transition cell cycle process	protein kinase complex transferase complex, transferring phosphorus-containing groups protein-containing complex catalytic complex serine/threonine protein kinase complex cyclin-dependent protein kinase holoenzyme complex transferase complex membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle cellular anatomical entity intracellular organelle lumen protein kinase complex transferase complex, transferring phosphorus-containing groups protein-containing complex catalytic complex serine/threonine protein kinase complex cyclin-dependent protein kinase holoenzyme complex transferase complex membrane-bounded organelle intracellular anatomical structure nucleus membrane-enclosed lumen intracellular membrane-bounded organelle cellular anatomical entity intracellular organelle lumen intracellular organelle nucleoplasm organelle lumen organelle nuclear lumen cullin-RING ubiquitin ligase complex ubiquitin ligase complex SCF ubiquitin ligase complex intracellular protein-containing complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR016073	SKP1 component, POZ domain	Domain
A	IPR001232	S-phase kinase-associated protein 1-like	Family
A	IPR036296	SKP1-like, dimerisation domain superfamily	Homologous Superfamily
A	IPR016072	SKP1 component, dimerisation	Domain
A	IPR011333	SKP1/BTB/POZ domain superfamily	Homologous Superfamily
A	IPR016897	S-phase kinase-associated protein 1	Family
B	IPR036047	F-box-like domain superfamily	Homologous Superfamily
B	IPR001810	F-box domain	Domain
B	IPR032675	Leucine-rich repeat domain superfamily	Homologous Superfamily
B	IPR006553	Leucine-rich repeat, cysteine-containing subtype	Repeat
C	IPR036858	Cyclin-dependent kinase, regulatory subunit superfamily	Homologous Superfamily
C	IPR000789	Cyclin-dependent kinase, regulatory subunit	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P63208	allergic rhinitis esophageal adenocarcinoma hypothyroidism medulloblastoma pilocytic astrocytoma subependymal giant cell astrocytoma atypical teratoid rhabdoid tumor glioblastoma large cell medulloblastoma ovarian cancer plasma cell myeloma systemic lupus erythematosus breast cancer Hashimoto thyroiditis malignant glioma allergic rhinitis esophageal adenocarcinoma hypothyroidism medulloblastoma pilocytic astrocytoma subependymal giant cell astrocytoma atypical teratoid rhabdoid tumor glioblastoma large cell medulloblastoma ovarian cancer plasma cell myeloma systemic lupus erythematosus breast cancer Hashimoto thyroiditis malignant glioma pancreatic ductal adenocarcinoma rheumatoid arthritis
B	Pharos: Q13309	medulloblastoma cancer diffuse large B-cell lymphoma childhood medulloblastoma medullomyoblastoma with myogenic differentiation melanotic medulloblastoma adult medulloblastoma nasopharyngeal carcinoma osteosarcoma pancreatic intraductal papillary-mucinous carcinoma psoriasis acute myeloid leukemia atypical teratoid rhabdoid tumor glioblastoma Liver carcinoma medulloblastoma cancer diffuse large B-cell lymphoma childhood medulloblastoma medullomyoblastoma with myogenic differentiation melanotic medulloblastoma adult medulloblastoma nasopharyngeal carcinoma osteosarcoma pancreatic intraductal papillary-mucinous carcinoma psoriasis acute myeloid leukemia atypical teratoid rhabdoid tumor glioblastoma Liver carcinoma malignant colon neoplasm malignant mesothelioma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm adrenal cortex carcinoma benign neoplasm desmoplastic/nodular medulloblastoma hepatocellular carcinoma lung cancer malignant ependymoma non-small cell lung carcinoma ovarian neoplasm primitive neuroectodermal tumor large cell medulloblastoma lymphoplasmacytic lymphoma malignant glioma
C	Pharos: P61024	atrial fibrillation cutaneous melanoma prostate carcinoma attention deficit hyperactivity disorder, inattentive type inflammatory bowel disease skin basal cell carcinoma breast carcinoma ovarian carcinoma

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.