2AS5

Structure of the DNA binding domains of NFAT and FOXP2 bound specifically to DNA.

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
E [auth N]	d2as5n1	All beta proteins	Immunoglobulin-like beta-sandwich	E set domains	NF-kappa-B/REL/DORSAL transcription factors, C-terminal domain	T-cell transcription factor NFAT1 (NFATC2)	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
E [auth N]	d2as5n2	All beta proteins	Common fold of diphtheria toxin/transcription factors/cytochrome f	p53-like transcription factors	Rel/Dorsal transcription factors, DNA-binding domain	T-cell transcription factor NFAT1 (NFATC), DNA-binding domain	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
F [auth M]	d2as5m1	All beta proteins	Immunoglobulin-like beta-sandwich	E set domains	NF-kappa-B/REL/DORSAL transcription factors, C-terminal domain	T-cell transcription factor NFAT1 (NFATC2)	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
F [auth M]	d2as5m2	All beta proteins	Common fold of diphtheria toxin/transcription factors/cytochrome f	p53-like transcription factors	Rel/Dorsal transcription factors, DNA-binding domain	T-cell transcription factor NFAT1 (NFATC), DNA-binding domain	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
G [auth F]	d2as5f_	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
H [auth G]	d2as5g_	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	automated matches	automated matches	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
E [auth N]	SCOP2B Superfamily	E set domains	8054859	3000070	SCOP2B (2022-06-29)
E [auth N]	SCOP2B Superfamily	p53-like transcription factors	8038345	3000758	SCOP2B (2022-06-29)
F [auth M]	SCOP2B Superfamily	E set domains	8054859	3000070	SCOP2B (2022-06-29)
F [auth M]	SCOP2B Superfamily	p53-like transcription factors	8038345	3000758	SCOP2B (2022-06-29)
G [auth F]	SCOP2B Superfamily	Winged helix DNA-binding domain	8057665	3000034	SCOP2B (2022-06-29)
H [auth G]	SCOP2B Superfamily	Winged helix DNA-binding domain	8057665	3000034	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
E [auth N]	RHD_dimer	e2as5N1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: RHD_dimer	ECOD (1.6)
E [auth N]	RHD_DNA_bind	e2as5N2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Common fold of diphtheria toxin/transcription factors/cytochrome f	F: RHD_DNA_bind	ECOD (1.6)
F [auth M]	RHD_dimer	e2as5M1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Immunoglobulin/Fibronectin type III/E set domains/PapD-like	F: RHD_dimer	ECOD (1.6)
F [auth M]	RHD_DNA_bind	e2as5M2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Common fold of diphtheria toxin/transcription factors/cytochrome f	F: RHD_DNA_bind	ECOD (1.6)
G [auth F]	Forkhead	e2as5F2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: Forkhead	ECOD (1.6)
H [auth G]	Forkhead	e2as5G2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: Forkhead	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
E [auth N]	2.60.40.340	Mainly Beta	Sandwich	Immunoglobulin-like	Rel homology domain (RHD), DNA-binding domain	CATH (4.3.0)
E [auth N]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
F [auth M]	2.60.40.340	Mainly Beta	Sandwich	Immunoglobulin-like	Rel homology domain (RHD), DNA-binding domain	CATH (4.3.0)
F [auth M]	2.60.40.10	Mainly Beta	Sandwich	Immunoglobulin-like	Immunoglobulins	CATH (4.3.0)
G [auth F]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
H [auth G]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
E [auth N], F [auth M]	PF16179	Rel homology dimerisation domain (RHD_dimer)	Rel homology dimerisation domain	The Rel homology domain (RHD) is composed of two structural domains, an N-terminal DNA_binding domain (Pfam:PF00554) and a C-terminal dimerisation domain. This is the dimerisation domain [1].	Domain
E [auth N], F [auth M]	PF00554	Rel homology DNA-binding domain (RHD_DNA_bind)	Rel homology DNA-binding domain	Proteins containing the Rel homology domain (RHD) are eukaryotic transcription factors. The RHD is composed of two structural domains. This is the N-terminal DNA-binding domain that is similar to that found in P53. The C-terminal domain has an immu ...	Proteins containing the Rel homology domain (RHD) are eukaryotic transcription factors. The RHD is composed of two structural domains. This is the N-terminal DNA-binding domain that is similar to that found in P53. The C-terminal domain has an immunoglobulin-like fold (See Pfam:PF16179) that functions as a dimerisation domain [1-2].	Domain
G [auth F], H [auth G]	PF00250	Forkhead domain (Forkhead)	Forkhead domain	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C	5'-D(TPTPAPGPGPAPAPAPAPTPTPTPGPTPTPTPCPAPTPAPGP*)-3'	-	-	-
B, D	5'-D(APAPCPTPAPTPGPAPAPAPCPAPAPAPTPTPTPTPCPCPTP*)-3'	-	-	-
E [auth N], F [auth M]	Nuclear factor of activated T-cells, cytoplasmic 2	DNA-binding transcription factor activity, RNA polymerase II-specific DNA-binding transcription repressor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription repressor activity DNA-binding transcription factor activity DNA binding sequence-specific DNA binding binding double-stranded DNA binding nucleic acid binding RNA polymerase II transcription regulatory region sequence-specific DNA binding cis-regulatory region sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding DNA-binding transcription factor activity, RNA polymerase II-specific DNA-binding transcription repressor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription repressor activity DNA-binding transcription factor activity DNA binding sequence-specific DNA binding binding double-stranded DNA binding nucleic acid binding RNA polymerase II transcription regulatory region sequence-specific DNA binding cis-regulatory region sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding organic cyclic compound binding transcription regulatory region nucleic acid binding RNA polymerase II cis-regulatory region sequence-specific DNA binding protein-containing complex binding chromatin binding protein binding 14-3-3 protein binding molecular adaptor activity phosphatase binding enzyme binding DNA-binding transcription activator activity, RNA polymerase II-specific DNA-binding transcription activator activity	developmental process multicellular organism development multicellular organismal process skeletal system development connective tissue development anatomical structure development animal organ development tissue development cartilage development system development cellular response to stimulus cellular response to stress response to stimulus DNA damage response developmental process multicellular organism development multicellular organismal process skeletal system development connective tissue development anatomical structure development animal organ development tissue development cartilage development system development cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cellular process response to chemical response to xenobiotic stimulus calcineurin-NFAT signaling cascade regulation of biological process regulation of cellular process signaling biological regulation intracellular signal transduction cell communication calcium-mediated signaling intracellular signaling cassette signal transduction calcineurin-mediated signaling immune response-regulating cell surface receptor signaling pathway positive regulation of biological process positive regulation of response to stimulus immune response-activating signaling pathway immune response-regulating signaling pathway positive regulation of immune response regulation of immune system process positive regulation of immune system process cell surface receptor signaling pathway immune response-activating cell surface receptor signaling pathway immune response B cell receptor signaling pathway regulation of response to stimulus immune system process regulation of immune response antigen receptor-mediated signaling pathway activation of immune response cellular biosynthetic process positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process positive regulation of metabolic process organic substance metabolic process positive regulation of biosynthetic process organic substance biosynthetic process positive regulation of macromolecule metabolic process positive regulation of cellular process regulation of biosynthetic process cellular metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process positive regulation of gene expression regulation of macromolecule biosynthetic process metabolic process macromolecule metabolic process regulation of metabolic process positive regulation of cellular biosynthetic process RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription organic cyclic compound metabolic process nucleic acid biosynthetic process transcription by RNA polymerase II organic cyclic compound biosynthetic process myotube cell development cellular developmental process cell development muscle cell differentiation myotube differentiation muscle structure development muscle cell development cell differentiation striated muscle cell differentiation cell migration cell motility cellular response to inorganic substance cellular response to metal ion response to inorganic substance cellular response to chemical stimulus response to metal ion cellular response to calcium ion response to calcium ion regulation of primary metabolic process regulation of RNA metabolic process regulation of nucleobase-containing compound metabolic process regulation of RNA biosynthetic process regulation of DNA-templated transcription positive regulation of nucleobase-containing compound metabolic process positive regulation of RNA metabolic process positive regulation of DNA-templated transcription positive regulation of RNA biosynthetic process positive regulation of cellular component organization regulation of cellular component organization regulation of syncytium formation by plasma membrane fusion regulation of developmental process cell-cell fusion cellular component organization positive regulation of developmental process cellular component organization or biogenesis syncytium formation positive regulation of myoblast fusion myoblast fusion regulation of myoblast fusion positive regulation of syncytium formation by plasma membrane fusion syncytium formation by plasma membrane fusion anatomical structure morphogenesis anatomical structure formation involved in morphogenesis smooth muscle cell differentiation negative regulation of biological process regulation of smooth muscle cell differentiation negative regulation of muscle cell differentiation regulation of cell differentiation vascular associated smooth muscle cell differentiation negative regulation of cellular process negative regulation of cell differentiation negative regulation of developmental process negative regulation of vascular associated smooth muscle cell differentiation regulation of vascular associated smooth muscle cell differentiation vasculature development circulatory system development negative regulation of smooth muscle cell differentiation regulation of muscle cell differentiation regulation of transcription by RNA polymerase II positive regulation of cell population proliferation positive regulation of B cell proliferation regulation of leukocyte activation regulation of cell activation lymphocyte activation leukocyte proliferation leukocyte activation positive regulation of lymphocyte activation positive regulation of cell activation regulation of B cell proliferation lymphocyte proliferation regulation of B cell activation positive regulation of leukocyte activation positive regulation of mononuclear cell proliferation regulation of multicellular organismal process regulation of lymphocyte proliferation regulation of leukocyte proliferation cell population proliferation B cell activation cell activation positive regulation of lymphocyte proliferation B cell proliferation positive regulation of B cell activation regulation of cell population proliferation mononuclear cell proliferation positive regulation of leukocyte proliferation regulation of mononuclear cell proliferation positive regulation of multicellular organismal process regulation of lymphocyte activation	RNA polymerase II transcription regulator complex membrane-bounded organelle protein-containing complex intracellular anatomical structure nucleus nuclear protein-containing complex transcription regulator complex intracellular membrane-bounded organelle cellular anatomical entity transcription factor AP-1 complex intracellular organelle organelle cytoplasm cytosol RNA polymerase II transcription regulator complex membrane-bounded organelle protein-containing complex intracellular anatomical structure nucleus nuclear protein-containing complex transcription regulator complex intracellular membrane-bounded organelle cellular anatomical entity transcription factor AP-1 complex intracellular organelle organelle cytoplasm cytosol membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen protein-DNA complex intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle ribonucleoprotein complex
G [auth F], H [auth G]	Forkhead box protein P2	DNA binding binding organic cyclic compound binding nucleic acid binding DNA-binding transcription factor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription factor activity identical protein binding protein binding protein homodimerization activity protein dimerization activity DNA-binding transcription repressor activity, RNA polymerase II-specific DNA-binding transcription repressor activity sequence-specific DNA binding DNA binding binding organic cyclic compound binding nucleic acid binding DNA-binding transcription factor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription factor activity identical protein binding protein binding protein homodimerization activity protein dimerization activity DNA-binding transcription repressor activity, RNA polymerase II-specific DNA-binding transcription repressor activity sequence-specific DNA binding double-stranded DNA binding RNA polymerase II transcription regulatory region sequence-specific DNA binding cis-regulatory region sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding transcription regulatory region nucleic acid binding RNA polymerase II cis-regulatory region sequence-specific DNA binding cation binding ion binding metal ion binding small molecule binding	regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process negative regulation of metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process organic substance metabolic process negative regulation of cellular process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process biological regulation regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process cellular process developmental process multicellular organismal process head development forebrain development animal organ development brain development nervous system development pallium development cerebral cortex development multicellular organism development central nervous system development anatomical structure development telencephalon development system development caudate nucleus development neural nucleus development striatum development subpallium development transcription by RNA polymerase II regulation of transcription by RNA polymerase II putamen development	intracellular organelle protein-containing complex intracellular anatomical structure protein-DNA complex organelle intracellular non-membrane-bounded organelle chromatin chromosome cellular anatomical entity non-membrane-bounded organelle membrane-bounded organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
E [auth N], F [auth M]	IPR037059	Rel homology domain (RHD), DNA-binding domain superfamily	Homologous Superfamily
E [auth N], F [auth M]	IPR002909	IPT domain	Domain
E [auth N], F [auth M]	IPR008967	p53-like transcription factor, DNA-binding domain superfamily	Homologous Superfamily
E [auth N], F [auth M]	IPR014756	Immunoglobulin E-set	Homologous Superfamily
E [auth N], F [auth M]	IPR011539	Rel homology domain, DNA-binding domain	Domain
E [auth N], F [auth M]	IPR008366	Nuclear factor of activated T cells (NFAT)	Family
E [auth N], F [auth M]	IPR032397	Rel homology dimerisation domain	Domain
E [auth N], F [auth M]	IPR013783	Immunoglobulin-like fold	Homologous Superfamily
G [auth F], H [auth G]	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
G [auth F], H [auth G]	IPR001766	Fork head domain	Domain
G [auth F], H [auth G]	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
G [auth F], H [auth G]	IPR030456	Fork head domain conserved site 2	Conserved Site
G [auth F], H [auth G]	IPR047412	Forkhead box protein P1/P2, forkhead domain	Domain
G [auth F], H [auth G]	IPR032354	FOXP, coiled-coil domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
E [auth N], F [auth M]	Pharos: Q13469	nervous system disorder pulmonary hypertension allergic rhinitis malignant ependymoma non-small cell lung carcinoma subependymal giant cell astrocytoma breast cancer medulloblastoma osteosarcoma type 2 diabetes mellitus interstitial cystitis ulcerative colitis
G [auth F], H [auth G]	Pharos: O15409	Silver-Russell syndrome childhood apraxia of speech adenoid cystic carcinoma Craniofacial Abnormalities agnosia salivary gland adenoid cystic carcinoma speech disorder ulcerative colitis gastroesophageal reflux disease intellectual disability large cell medulloblastoma malignant ependymoma oligodendroglioma pancreatic intraductal papillary-mucinous carcinoma astrocytic tumor Silver-Russell syndrome childhood apraxia of speech adenoid cystic carcinoma Craniofacial Abnormalities agnosia salivary gland adenoid cystic carcinoma speech disorder ulcerative colitis gastroesophageal reflux disease intellectual disability large cell medulloblastoma malignant ependymoma oligodendroglioma pancreatic intraductal papillary-mucinous carcinoma astrocytic tumor periodontitis auditory perceptual disorders schizophrenia breast carcinoma sinusitis ductal breast carcinoma in situ glioblastoma interstitial cystitis lung carcinoma malignant glioma ovarian cancer pancreatic intraductal papillary-mucinous neoplasm attention deficit hyperactivity disorder, inattentive type pilocytic astrocytoma autism breast fibroadenoma cystic fibrosis transitional cell carcinoma dyslexia invasive ductal breast carcinoma apraxia language disorder major depressive disorder medulloblastoma pancreatic ductal adenocarcinoma pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia atypical teratoid rhabdoid tumor

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.