2A6T

Crystal structure of S.pombe mRNA decapping enzyme Dcp2p

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
B	d2a6tb1	Alpha and beta proteins (a+b)	Nudix	Nudix	mRNA decapping enzyme-like	mRNA decapping enzyme Dcp2p catalytic domain	fission yeast (Schizosaccharomyces pombe ) [TaxId: 4896 ],	SCOPe (2.08)
A	d2a6ta1	All alpha proteins	Dcp2 domain-like	Dcp2 domain-like	Dcp2 box A domain	mRNA decapping enzyme Dcp2p, N-terminal domain	fission yeast (Schizosaccharomyces pombe ) [TaxId: 4896 ],	SCOPe (2.08)
A	d2a6ta2	Alpha and beta proteins (a+b)	Nudix	Nudix	mRNA decapping enzyme-like	mRNA decapping enzyme Dcp2p catalytic domain	fission yeast (Schizosaccharomyces pombe ) [TaxId: 4896 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2B Superfamily	Nudix	8036684	3000098	SCOP2B (2022-06-29)
A	SCOP2 Family	Dcp2-like	8023692	4001346	SCOP2 (2022-06-29)
A	SCOP2 Family	mRNA decapping enzyme-like	8024305	4003955	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Dcp2 domain-like	8036072	3001348	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Nudix	8036684	3000098	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	PF05026	e2a6tB2	A: alpha arrays	X: Dcp2 box A domain (From Topology)	H: Dcp2 box A domain (From Topology)	T: Dcp2 box A domain	F: PF05026	ECOD (1.6)
B	PF00293	e2a6tB1	A: a+b two layers	X: beta-Grasp	H: Nudix (From Topology)	T: Nudix	F: PF00293	ECOD (1.6)
A	PF05026	e2a6tA1	A: alpha arrays	X: Dcp2 box A domain (From Topology)	H: Dcp2 box A domain (From Topology)	T: Dcp2 box A domain	F: PF05026	ECOD (1.6)
A	PF00293	e2a6tA2	A: a+b two layers	X: beta-Grasp	H: Nudix (From Topology)	T: Nudix	F: PF00293	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
B	1.10.10.1050	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Dcp2, box A domain	CATH (4.3.0)
B	3.90.79.10	Alpha Beta	Alpha-Beta Complex	Nucleoside Triphosphate Pyrophosphohydrolase	Nucleoside Triphosphate Pyrophosphohydrolase	CATH (4.3.0)
A	1.10.10.1050	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Dcp2, box A domain	CATH (4.3.0)
A	3.90.79.10	Alpha Beta	Alpha-Beta Complex	Nucleoside Triphosphate Pyrophosphohydrolase	Nucleoside Triphosphate Pyrophosphohydrolase	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00293	NUDIX domain (NUDIX)	NUDIX domain	-	Domain
A, B	PF05026	Dcp2, box A domain (DCP2)	Dcp2, box A domain	This domain is always found to the amino terminal side of Pfam:PF00293. This domain is specific to mRNA decapping protein 2 and this region has been termed Box A [2]. Removal of the cap structure is catalysed by the Dcp1-Dcp2 complex [3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	SPAC19A8.12	RNA binding mRNA cap binding binding organic cyclic compound binding nucleic acid binding RNA cap binding mRNA binding cation binding manganese ion binding transition metal ion binding ion binding metal ion binding small molecule binding single-stranded RNA binding RNA binding mRNA cap binding binding organic cyclic compound binding nucleic acid binding RNA cap binding mRNA binding cation binding manganese ion binding transition metal ion binding ion binding metal ion binding small molecule binding single-stranded RNA binding magnesium ion binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding pyrophosphatase activity 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity 5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity	regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of metabolic process post-transcriptional regulation of gene expression regulation of RNA metabolic process organic substance biosynthetic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process regulation of biological quality RNA catabolic process mRNA catabolic process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of metabolic process post-transcriptional regulation of gene expression regulation of RNA metabolic process organic substance biosynthetic process positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process regulation of biological quality RNA catabolic process mRNA catabolic process regulation of biological process positive regulation of nucleobase-containing compound metabolic process cellular metabolic process negative regulation of metabolic process positive regulation of mRNA metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process nucleobase-containing compound catabolic process positive regulation of RNA metabolic process organic cyclic compound metabolic process regulation of gene expression negative regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process nucleic acid catabolic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process deadenylation-dependent decapping of nuclear-transcribed mRNA regulation of metabolic process RNA decapping nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay catabolic process cellular biosynthetic process positive regulation of biological process RNA metabolic process macromolecule catabolic process mRNA destabilization organic substance metabolic process regulation of mRNA catabolic process negative regulation of cellular process mRNA methylguanosine-cap decapping negative regulation of macromolecule metabolic process regulation of biosynthetic process nuclear-transcribed mRNA catabolic process RNA destabilization biological regulation regulation of macromolecule metabolic process regulation of catabolic process gene expression mRNA metabolic process macromolecule biosynthetic process nucleic acid metabolic process organic cyclic compound catabolic process regulation of cellular biosynthetic process positive regulation of catabolic process regulation of mRNA stability negative regulation of biosynthetic process positive regulation of mRNA catabolic process regulation of RNA stability cellular process regulation of mRNA metabolic process organic substance catabolic process mRNA processing RNA processing nuclear-transcribed mRNA catabolic process, nonsense-mediated decay	intracellular anatomical structure cytoplasmic ribonucleoprotein granule cellular anatomical entity ribonucleoprotein granule intracellular organelle organelle intracellular non-membrane-bounded organelle cytoplasm supramolecular complex non-membrane-bounded organelle cytoplasmic stress granule cytosol protein-containing complex RNA decapping complex intracellular anatomical structure cytoplasmic ribonucleoprotein granule cellular anatomical entity ribonucleoprotein granule intracellular organelle organelle intracellular non-membrane-bounded organelle cytoplasm supramolecular complex non-membrane-bounded organelle cytoplasmic stress granule cytosol protein-containing complex RNA decapping complex P-body membrane-bounded organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR015797	NUDIX hydrolase-like domain superfamily	Homologous Superfamily
A, B	IPR036189	mRNA decapping protein 2, Box A domain superfamily	Homologous Superfamily
A, B	IPR007722	mRNA decapping protein 2, Box A domain	Domain
A, B	IPR044099	mRNA decapping enzyme 2 , NUDIX hydrolase domain	Domain
A, B	IPR000086	NUDIX hydrolase domain	Domain
A, B	IPR020084	NUDIX hydrolase, conserved site	Conserved Site

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.