Annotations: 2A5Y

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2a5ya_	Membrane and cell surface proteins and peptides	Toxins' membrane translocation domains	Bcl-2 inhibitors of programmed cell death	Bcl-2 inhibitors of programmed cell death	automated matches	(Caenorhabditis elegans ) [TaxId: 6239 ],	SCOPe (2.08)
B	d2a5yb1	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	CED-4 C-terminal domain-like	Cell death protein 4, CED-4	(Caenorhabditis elegans ) [TaxId: 6239 ],	SCOPe (2.08)
B	d2a5yb2	All alpha proteins	DEATH domain	DEATH domain	Caspase recruitment domain, CARD	Cell death protein 4, CED-4	(Caenorhabditis elegans ) [TaxId: 6239 ],	SCOPe (2.08)
B	d2a5yb3	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	Extended AAA-ATPase domain	CED-4, NB-ARC domain	(Caenorhabditis elegans ) [TaxId: 6239 ],	SCOPe (2.08)
C	d2a5yc2	All alpha proteins	DNA/RNA-binding 3-helical bundle	'Winged helix' DNA-binding domain	CED-4 C-terminal domain-like	Cell death protein 4, CED-4	(Caenorhabditis elegans ) [TaxId: 6239 ],	SCOPe (2.08)
C	d2a5yc1	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	Extended AAA-ATPase domain	CED-4, NB-ARC domain	(Caenorhabditis elegans ) [TaxId: 6239 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Bcl-2-like inhibitors of programmed cell death	8037483	3001974	SCOP2B (2022-06-29)
B	SCOP2 Family	CED-4 C-terminal domain-like	8058308	4000210	SCOP2 (2022-06-29)
B	SCOP2 Family	CARD-like	8003076	4000308	SCOP2 (2022-06-29)
B	SCOP2 Family	Extended AAA-ATPase domain	8003073	4000283	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Winged helix DNA-binding domain	8003079	3000034	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	DEATH domain	8003077	3000069	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	DNA polymerases epsilon N-terminal domain (Dpoe2NT)-like	8104155	3002834	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	RecA-like P-loop NTPases	8003074	3002019	SCOP2 (2022-06-29)
C	SCOP2B Superfamily	DNA polymerases epsilon N-terminal domain (Dpoe2NT)-like	8104155	3002834	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Winged helix DNA-binding domain	8003079	3000034	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	RecA-like P-loop NTPases	8003074	3002019	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00452,PF02180	e2a5yA1	A: alpha arrays	X: Toxins' membrane translocation domains	H: Bcl-2 inhibitors of programmed cell death (From Topology)	T: Bcl-2 inhibitors of programmed cell death	F: PF00452,PF02180	ECOD (1.6)
B	PF22094	e2a5yB1	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF22094	ECOD (1.6)
B	PF00619	e2a5yB2	A: alpha arrays	X: DEATH domain	H: DEATH domain (From Topology)	T: DEATH domain	F: PF00619	ECOD (1.6)
B	PF00931	e2a5yB4	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: PF00931	ECOD (1.6)
B	PF00931	e2a5yB5	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00931	ECOD (1.6)
C	PF22094	e2a5yC2	A: alpha arrays	X: HTH	H: HTH	T: winged	F: PF22094	ECOD (1.6)
C	PF00931	e2a5yC4	A: alpha arrays	X: Histone-like	H: Histone-related	T: AAA+ ATPase lid domain	F: PF00931	ECOD (1.6)
C	PF00931	e2a5yC3	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00931	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.437.10	Mainly Alpha	Orthogonal Bundle	Apoptosis Regulator Bcl-x	Blc2-like	CATH (4.3.0)
B	1.10.533.10	Mainly Alpha	Orthogonal Bundle	Death Domain, Fas	Death Domain, Fas	CATH (4.3.0)
B	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
B	1.10.8.490	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
B	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
C	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
C	1.10.8.490	Mainly Alpha	Orthogonal Bundle	Helicase, Ruva Protein	domain 3	CATH (4.3.0)
C	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF02180	Bcl-2 homology region 4 (BH4)	Bcl-2 homology region 4	-	Family
A	PF00452	Apoptosis regulator proteins, Bcl-2 family (Bcl-2)	Apoptosis regulator proteins, Bcl-2 family	-	Family
B, C	PF00931	NB-ARC domain (NB-ARC)	NB-ARC domain	-	Domain
B, C	PF00619	Caspase recruitment domain (CARD)	Caspase recruitment domain	Motif contained in proteins involved in apoptotic signaling. Predicted to possess a DEATH (Pfam:PF00531) domain-like fold .	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Apoptosis regulator ced-9	protein heterodimerization activity protein binding binding protein dimerization activity GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity molecular function activator activity nucleoside-triphosphatase regulator activity molecular function regulator activity molecular sequestering activity protein sequestering activity identical protein binding protein heterodimerization activity protein binding binding protein dimerization activity GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity molecular function activator activity nucleoside-triphosphatase regulator activity molecular function regulator activity molecular sequestering activity protein sequestering activity identical protein binding protein homodimerization activity	cellular component disassembly regulation of synapse pruning regulation of biological process positive regulation of cellular component organization regulation of cellular process regulation of cellular component organization cell junction disassembly synapse pruning biological regulation positive regulation of biological process positive regulation of synapse pruning cellular component organization regulation of synapse organization cell junction organization cellular component disassembly regulation of synapse pruning regulation of biological process positive regulation of cellular component organization regulation of cellular process regulation of cellular component organization cell junction disassembly synapse pruning biological regulation positive regulation of biological process positive regulation of synapse pruning cellular component organization regulation of synapse organization cell junction organization cellular process regulation of synapse structure or activity synapse organization cellular component organization or biogenesis regulation of biological quality positive regulation of cellular process apoptotic process involved in development developmental process anatomical structure development apoptotic process cell death programmed cell death negative regulation of programmed cell death regulation of apoptotic process negative regulation of biological process regulation of programmed cell death negative regulation of apoptotic process negative regulation of cellular process positive regulation of programmed cell death positive regulation of apoptotic process actin polymerization or depolymerization actin cytoskeleton organization protein-containing complex organization actin filament organization protein-containing complex disassembly actin filament depolymerization organelle organization actin filament-based process supramolecular fiber organization protein depolymerization cytoskeleton organization organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process primary metabolic process gene expression macromolecule biosynthetic process protein maturation organic substance metabolic process biosynthetic process proteolysis organic substance biosynthetic process protein processing metabolic process protein metabolic process macromolecule metabolic process negative regulation of cellular component organization regulation of cellular component biogenesis negative regulation of protein-containing complex assembly cellular component biogenesis regulation of protein-containing complex assembly cellular component assembly protein-containing complex assembly negative regulation of execution phase of apoptosis regulation of execution phase of apoptosis execution phase of apoptosis cellular response to stimulus signal transduction in absence of ligand response to stimulus cell surface receptor signaling pathway signaling extrinsic apoptotic signaling pathway cell communication apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand signal transduction positive regulation of organelle organization regulation of developmental process positive regulation of developmental process regulation of mitochondrial fusion mitochondrial fusion regulation of anatomical structure morphogenesis positive regulation of mitochondrial fusion mitochondrion organization regulation of organelle organization organelle fusion anatomical structure morphogenesis regulation of mitochondrion organization intracellular signal transduction cellular response to stress DNA damage response response to stress intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway biological process involved in interspecies interaction between organisms response to other organism defense response response to external stimulus defense response to Gram-negative bacterium response to biotic stimulus defense response to bacterium defense response to other organism response to bacterium response to external biotic stimulus sexual reproduction regulation of reproductive process reproductive process fertilization regulation of fertilization positive regulation of fertilization positive regulation of reproductive process multicellular organismal process regulation of embryonic development regulation of multicellular organismal development embryo development multicellular organism development positive regulation of embryonic development positive regulation of multicellular organismal process regulation of multicellular organismal process catabolic process macroautophagy autophagy cellular catabolic process mitophagy organelle disassembly autophagy of mitochondrion process utilizing autophagic mechanism egg-laying behavior multicellular organismal reproductive process regulation of egg-laying behavior regulation of behavior behavior positive regulation of behavior positive regulation of egg-laying behavior reproductive behavior multi-multicellular organism process positive regulation of brood size regulation of brood size	cellular anatomical entity endomembrane system membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle somatodendritic compartment cell body perikaryon neuronal cell body membrane cellular anatomical entity endomembrane system membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm intracellular membrane-bounded organelle somatodendritic compartment cell body perikaryon neuronal cell body membrane organelle membrane presynapse synapse cell junction perinuclear region of cytoplasm mitochondrial membrane organelle outer membrane organelle envelope bounding membrane of organelle mitochondrial envelope mitochondrial outer membrane outer membrane
B, C	ced-4	protein binding binding BH1 domain binding BH domain binding protein domain specific binding cation binding magnesium ion binding ion binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding protein binding binding BH1 domain binding BH domain binding protein domain specific binding cation binding magnesium ion binding ion binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding ADP binding death domain binding BH3 domain binding enzyme binding protease binding caspase binding enzyme regulator activity enzyme activator activity peptidase regulator activity molecular function activator activity cysteine-type endopeptidase activator activity involved in apoptotic process molecular function regulator activity cysteine-type endopeptidase regulator activity involved in apoptotic process peptidase activator activity peptidase activator activity involved in apoptotic process endopeptidase activator activity endopeptidase regulator activity identical protein binding	cellular component disassembly regulation of synapse pruning regulation of biological process positive regulation of cellular component organization regulation of cellular process regulation of cellular component organization cell junction disassembly synapse pruning biological regulation positive regulation of biological process positive regulation of synapse pruning cellular component organization regulation of synapse organization cell junction organization cellular component disassembly regulation of synapse pruning regulation of biological process positive regulation of cellular component organization regulation of cellular process regulation of cellular component organization cell junction disassembly synapse pruning biological regulation positive regulation of biological process positive regulation of synapse pruning cellular component organization regulation of synapse organization cell junction organization cellular process regulation of synapse structure or activity synapse organization cellular component organization or biogenesis regulation of biological quality positive regulation of cellular process cell adhesion regulation of cell adhesion apoptotic process involved in development developmental process anatomical structure development apoptotic process cell death programmed cell death activation of cysteine-type endopeptidase activity regulation of catalytic activity cellular biosynthetic process positive regulation of protein metabolic process positive regulation of peptidase activity protein maturation regulation of primary metabolic process positive regulation of molecular function positive regulation of metabolic process organic substance metabolic process regulation of proteolysis proteolysis regulation of endopeptidase activity organic substance biosynthetic process positive regulation of macromolecule metabolic process protein metabolic process zymogen activation regulation of cysteine-type endopeptidase activity organonitrogen compound metabolic process cellular metabolic process regulation of protein metabolic process regulation of peptidase activity positive regulation of catalytic activity primary metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of molecular function positive regulation of hydrolase activity biosynthetic process positive regulation of cysteine-type endopeptidase activity regulation of hydrolase activity positive regulation of endopeptidase activity positive regulation of proteolysis protein processing metabolic process macromolecule metabolic process regulation of metabolic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of cysteine-type endopeptidase activity involved in apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of programmed cell death regulation of apoptotic process negative regulation of biological process regulation of programmed cell death negative regulation of apoptotic process negative regulation of cellular process regulation of cellular component size regulation of cell size regulation of anatomical structure size positive regulation of programmed cell death positive regulation of apoptotic process actin polymerization or depolymerization actin cytoskeleton organization protein-containing complex organization actin filament organization protein-containing complex disassembly actin filament depolymerization organelle organization actin filament-based process supramolecular fiber organization protein depolymerization cytoskeleton organization regulation of developmental process positive regulation of apoptotic process involved in development regulation of apoptotic process involved in development positive regulation of developmental process regulation of protein maturation positive regulation of protein maturation positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process positive regulation of protein processing positive regulation of biosynthetic process regulation of biosynthetic process regulation of cellular metabolic process regulation of cellular biosynthetic process regulation of gene expression positive regulation of gene expression regulation of protein processing regulation of macromolecule biosynthetic process positive regulation of cellular biosynthetic process negative regulation of execution phase of apoptosis regulation of execution phase of apoptosis execution phase of apoptosis multicellular organism development multicellular organismal process anatomical structure morphogenesis embryonic morphogenesis embryo development regulation of protein stability biological process involved in interspecies interaction between organisms response to stimulus response to other organism defense response response to external stimulus defense response to Gram-negative bacterium response to biotic stimulus defense response to bacterium defense response to other organism response to stress response to bacterium response to external biotic stimulus regulation of development, heterochronic embryo development ending in birth or egg hatching cellular homeostasis homeostatic process muscle cell cellular homeostasis	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle membrane protein-containing complex catalytic complex caspase complex peptidase complex endopeptidase complex membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle membrane protein-containing complex catalytic complex caspase complex peptidase complex endopeptidase complex cytosol perinuclear region of cytoplasm nucleus

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR036834	Bcl-2-like superfamily	Homologous Superfamily
A	IPR046371	Bcl-2, Bcl-2 homology region 1-3	Domain
A	IPR003093	Apoptosis regulator, Bcl-2 protein, BH4	Domain
A	IPR002475	Bcl2-like	Family
A	IPR026298	Bcl-2 family	Family
A	IPR020717	Apoptosis regulator, Bcl-2, BH1 motif, conserved site	Conserved Site
B, C	IPR002182	NB-ARC	Domain
B, C	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
B, C	IPR011029	Death-like domain superfamily	Homologous Superfamily
B, C	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
B, C	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
B, C	IPR016854	Apoptosis regulator, Ced-4	Family
B, C	IPR001315	CARD domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.