1VIE
STRUCTURE OF DIHYDROFOLATE REDUCTASE
External Resource: Annotation
Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage
Chains | Domain Info | Class | Fold | Superfamily | Family | Domain | Species | Provenance Source (Version) |
---|---|---|---|---|---|---|---|---|
A | d1viea_ | All beta proteins | SH3-like barrel | Electron transport accessory proteins | R67 dihydrofolate reductase | R67 dihydrofolate reductase | (Escherichia coli ) [TaxId: 562 ], | SCOPe (2.08) |
Domain Annotation: SCOP2 Classification SCOP2 Database Homepage
Chains | Type | Family Name | Domain Identifier | Family Identifier | Provenance Source (Version) |
---|---|---|---|---|---|
A | SCOP2B Superfamily | Electron transport accessory proteins | 8036825 | 3000325 | SCOP2B (2022-06-29) |
Domain Annotation: ECOD Classification ECOD Database Homepage
Domain Annotation: CATH CATH Database Homepage
Chain | Domain | Class | Architecture | Topology | Homology | Provenance Source (Version) |
---|---|---|---|---|---|---|
A | 2.30.30.60 | Mainly Beta | Roll | SH3 type barrels. | CATH (4.3.0) |
Protein Family Annotation Pfam Database Homepage
Chains | Accession | Name | Description | Comments | Source |
---|---|---|---|---|---|
PF06442 | R67 dihydrofolate reductase (DHFR_2) | R67 dihydrofolate reductase | R67 dihydrofolate reductase is a plasmid encoded enzyme that provides resistance to the antibacterial drug trimethoprim. The R67 dihydrofolate reductase does not share significant similarity to the chromosomal encoded dihydrofolate reductase [1]. | Domain |
Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage
InterPro: Protein Family Classification InterPro Database Homepage
Chains | Accession | Name | Type |
---|---|---|---|
IPR008990 | Electron transport accessory-like domain superfamily | Homologous Superfamily | |
IPR009159 | Dihydrofolate reductase, type II | Family | |
IPR023408 | Mechanosensitive ion channel MscS, beta-domain superfamily | Homologous Superfamily |
Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage
Chains | Enzyme Name | Description | Catalytic Residues |
---|---|---|---|
dihydrofolate reductase (type II) M-CSA #752 | R67-plasmid encoded dihydrofolate reductase (R67-DHFR) from Escherichia coli catalyses the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate (THF) in the presence of NADPH. It is one of the smallest enzymes known to self-assemble into an active quaternary structure. The tetramer has an unusual pore, 25 angstroms in length that passes through the middle of the molecule and out the other side. R67-DHFR has 222 symmetry, and has a distinctive 'one site fits both' substrate and cofactor. | Defined by 4 residues: LYS:A-16 [auth A-32]GLN:A-51 [auth A-67]ILE:A-52 [auth A-68]TYR:A-53 [auth A-69] | EC: 1.5.1.3 (PDB Primary Data) |