Annotations: 1VIE

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1viea_	All beta proteins	SH3-like barrel	Electron transport accessory proteins	R67 dihydrofolate reductase	R67 dihydrofolate reductase	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Electron transport accessory proteins	8036825	3000325	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	DHFR_2	e1vieA1	A: beta barrels	X: SH3	H: SH3	T: SH3	F: DHFR_2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.30.30.60	Mainly Beta	Roll	SH3 type barrels.		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF06442	R67 dihydrofolate reductase (DHFR_2)	R67 dihydrofolate reductase	R67 dihydrofolate reductase is a plasmid encoded enzyme that provides resistance to the antibacterial drug trimethoprim. The R67 dihydrofolate reductase does not share significant similarity to the chromosomal encoded dihydrofolate reductase [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	DIHYDROFOLATE REDUCTASE	dihydrofolate reductase activity oxidoreductase activity oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on the CH-NH group of donors catalytic activity	response to stimulus response to chemical response to xenobiotic stimulus response to methotrexate response to organic substance response to organonitrogen compound response to oxygen-containing compound response to nitrogen compound response to organic cyclic compound cellular metabolic process small molecule metabolic process metabolic process cellular process one-carbon metabolic process response to stimulus response to chemical response to xenobiotic stimulus response to methotrexate response to organic substance response to organonitrogen compound response to oxygen-containing compound response to nitrogen compound response to organic cyclic compound cellular metabolic process small molecule metabolic process metabolic process cellular process one-carbon metabolic process folic acid-containing compound biosynthetic process organonitrogen compound metabolic process organonitrogen compound biosynthetic process cellular modified amino acid metabolic process organic cyclic compound metabolic process organic substance metabolic process biosynthetic process cellular modified amino acid biosynthetic process organic cyclic compound biosynthetic process folic acid-containing compound metabolic process pteridine-containing compound biosynthetic process organic substance biosynthetic process tetrahydrofolate biosynthetic process pteridine-containing compound metabolic process tetrahydrofolate metabolic process response to antibiotic	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR008990	Electron transport accessory-like domain superfamily	Homologous Superfamily
A	IPR009159	Dihydrofolate reductase, type II	Family
A	IPR023408	Mechanosensitive ion channel MscS, beta-domain superfamily	Homologous Superfamily

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	dihydrofolate reductase (type II) M-CSA #752	R67-plasmid encoded dihydrofolate reductase (R67-DHFR) from Escherichia coli catalyses the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate (THF) in the presence of NADPH. It is one of the smallest enzymes known to self-assemble into an active quaternary structure. The tetramer has an unusual pore, 25 angstroms in length that passes through the middle of the molecule and out the other side. R67-DHFR has 222 symmetry, and has a distinctive 'one site fits both' substrate and cofactor.	Defined by 4 residues: LYS:A-16 [auth A-32]GLN:A-51 [auth A-67]ILE:A-52 [auth A-68]TYR:A-53 [auth A-69] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 1.5.1.3 (PDB Primary Data) Search M-CSA Motif + EC 1.5.1.3

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