1ZUN

Crystal Structure of a GTP-Regulated ATP Sulfurylase Heterodimer from Pseudomonas syringae

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1zuna1	Alpha and beta proteins (a/b)	Adenine nucleotide alpha hydrolase-like	Adenine nucleotide alpha hydrolases-like	PAPS reductase-like	Sulfate adenylyltransferase subunit 2, CysD	(Pseudomonas syringae ) [TaxId: 317 ],	SCOPe (2.08)
A	d1zuna2	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Pseudomonas syringae ) [TaxId: 317 ],	SCOPe (2.08)
B	d1zunb1	All beta proteins	Reductase/isomerase/elongation factor common domain	Translation proteins	Elongation factors	Sulfate adenylate transferase subunit cysN/C, EF-Tu domain 2-like domain	(Pseudomonas syringae pv. tomato str. DC3000 ) [TaxId: 223283 ],	SCOPe (2.08)
B	d1zunb2	All beta proteins	Elongation factor/aminomethyltransferase common domain	EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain	EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain	Sulfate adenylate transferase subunit cysN/C, EF-Tu domain 3-like domain	(Pseudomonas syringae pv. tomato str. DC3000 ) [TaxId: 223283 ],	SCOPe (2.08)
B	d1zunb3	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	Sulfate adenylate transferase subunit cysN/C, EF-Tu domain G-like domain	(Pseudomonas syringae pv. tomato str. DC3000 ) [TaxId: 223283 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	PAPS reductase-like	8023903	4003838	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Adenine nucleotide alpha hydrolases-like	8036283	3001593	SCOP2 (2022-06-29)
B	SCOP2 Family	Translation factor common region-like	8023909	4004050	SCOP2 (2022-06-29)
B	SCOP2 Family	EF-Tu C-terminal domain-like	8023907	4002110	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	Ras-like P-loop GTPases	8036288	3002022	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	EF-Tu post-G domain-like	8036286	3002024	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	EF-Tu post-G domain-like	8044576	3002024	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF01507	e1zunA1	A: a/b three-layered sandwiches	X: HUP domain-like	H: HUP domains (From Topology)	T: HUP domains	F: PF01507	ECOD (1.6)
B	PF03144	e1zunB1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Alanine racemase-C	F: PF03144	ECOD (1.6)
B	PF22594	e1zunB2	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: Aminomethyltransferase beta-barrel domain	F: PF22594	ECOD (1.6)
B	PF00009	e1zunB3	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: PF00009	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.620	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	HUPs	CATH (4.3.0)
B	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
B	2.40.30.10	Mainly Beta	Beta Barrel	Elongation Factor Tu (Ef-tu)	domain 3	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF01507	Phosphoadenosine phosphosulfate reductase family (PAPS_reduct)	Phosphoadenosine phosphosulfate reductase family	-	Family
B	PF00009	Elongation factor Tu GTP binding domain (GTP_EFTU)	Elongation factor Tu GTP binding domain	This domain contains a P-loop motif, also found in several other families such as Pfam:PF00071, Pfam:PF00025 and Pfam:PF00063. Elongation factor Tu consists of three structural domains, this plus two C-terminal beta barrel domains.	Domain
B	PF03144	Elongation factor Tu domain 2 (GTP_EFTU_D2)	Elongation factor Tu domain 2	Elongation factor Tu consists of three structural domains, this is the second domain. This domain adopts a beta barrel structure. This the second domain is involved in binding to charged tRNA [1]. This domain is also found in other proteins such as e ...	Elongation factor Tu consists of three structural domains, this is the second domain. This domain adopts a beta barrel structure. This the second domain is involved in binding to charged tRNA [1]. This domain is also found in other proteins such as elongation factor G and translation initiation factor IF-2. This domain is structurally related to Pfam:PF03143, and in fact has weak sequence matches to this domain.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Sulfate adenylyltransferase subunit 2	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding adenylyltransferase activity transferase activity sulfate adenylyltransferase (ATP) activity sulfate adenylyltransferase activity nucleotidyltransferase activity catalytic activity transferase activity, transferring phosphorus-containing groups	sulfur compound biosynthetic process biosynthetic process cellular biosynthetic process cellular metabolic process sulfur compound metabolic process metabolic process cellular process hydrogen sulfide biosynthetic process hydrogen sulfide metabolic process sulfate assimilation	-
B	sulfate adenylate transferase, subunit 1/adenylylsulfate kinase	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding adenylyltransferase activity transferase activity sulfate adenylyltransferase (ATP) activity sulfate adenylyltransferase activity nucleotidyltransferase activity catalytic activity transferase activity, transferring phosphorus-containing groups guanosine tetraphosphate binding guanyl nucleotide binding guanyl ribonucleotide binding kinase activity adenylylsulfate kinase activity phosphotransferase activity, alcohol group as acceptor GTP binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides	phosphorylation cellular metabolic process phosphate-containing compound metabolic process metabolic process cellular process phosphorus metabolic process sulfur compound biosynthetic process biosynthetic process cellular biosynthetic process sulfur compound metabolic process hydrogen sulfide biosynthetic process hydrogen sulfide metabolic process sulfate assimilation	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR011784	Sulphate adenylyltransferase, small subunit	Family
A	IPR002500	Phosphoadenosine phosphosulphate reductase	Domain
A	IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	Homologous Superfamily
B	IPR000795	Translational (tr)-type GTP-binding domain	Domain
B	IPR009001	Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal	Homologous Superfamily
B	IPR031157	Tr-type G domain, conserved site	Conserved Site
B	IPR044138	Sulfate adenylyltransferase subunit CysN, Domain II	Domain
B	IPR004161	Translation elongation factor EFTu-like, domain 2	Domain
B	IPR011779	Sulphate adenylyltransferase, large subunit	Family
B	IPR044139	Sulfate adenylyltransferase subunit CysN, Domain III	Domain
B	IPR009000	Translation protein, beta-barrel domain superfamily	Homologous Superfamily
B	IPR002891	Adenylyl-sulfate kinase	Family
B	IPR005225	Small GTP-binding protein domain	Domain
B	IPR041757	Sulfate adenylyltransferase subunit CysN, GTP-binding domain	Domain
B	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.