Annotations: 1Y64

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1y64a2	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	Actin/HSP70	automated matches	(Oryctolagus cuniculus ) [TaxId: 9986 ],	SCOPe (2.08)
A	d1y64a1	Alpha and beta proteins (a/b)	Ribonuclease H-like motif	Actin-like ATPase domain	automated matches	automated matches	(Oryctolagus cuniculus ) [TaxId: 9986 ],	SCOPe (2.08)
B	d1y64b_	All alpha proteins	Formin homology 2 domain (FH2 domain)	Formin homology 2 domain (FH2 domain)	Formin homology 2 domain (FH2 domain)	Bni1	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Actin-like ATPases	8040509	3000092	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Actin-like ATPases	8040506	3000092	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Formin homology 2 domain (FH2 domain)	8035262	3001266	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Actin_1st	e1y64A1	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_1st	ECOD (1.6)
A	Actin_2nd	e1y64A2	A: mixed a+b and a/b	X: Ribonuclease H-like	H: Ribonuclease H-like (From Topology)	T: Ribonuclease H-like	F: Actin_2nd	ECOD (1.6)
B	FH2	e1y64B1	A: alpha complex topology	X: Formin homology 2 domain (FH2 domain) (From Topology)	H: Formin homology 2 domain (FH2 domain) (From Topology)	T: Formin homology 2 domain (FH2 domain)	F: FH2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.420.40	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
A	3.90.640.10	Alpha Beta	Alpha-Beta Complex	Actin	Chain A, domain 4	CATH (4.3.0)
B	6.10.30.50	Special	Helix non-globular	Lyase 2-enoyl-coa Hydratase	Chain A, domain 2	CATH (4.3.0)
B	1.20.58.2220	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00022	Actin (Actin)	Actin	-	Family
B	PF02181	Formin Homology 2 Domain (FH2)	Formin Homology 2 Domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Actin, alpha skeletal muscle	cytoskeletal protein binding titin binding protein binding binding cation binding ion binding calcium ion binding metal ion binding small molecule binding protein domain specific binding hydrolase activity catalytic activity troponin I binding cytoskeletal motor activator activity cytoskeletal protein binding titin binding protein binding binding cation binding ion binding calcium ion binding metal ion binding small molecule binding protein domain specific binding hydrolase activity catalytic activity troponin I binding cytoskeletal motor activator activity cytoskeletal motor regulator activity molecular function activator activity molecular function regulator activity magnesium ion binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding myosin heavy chain binding myosin binding tropomyosin binding calcium-dependent protein binding identical protein binding actin monomer binding actin binding	developmental process mesenchyme migration multicellular organismal process mesenchyme morphogenesis animal organ development mesenchyme development tissue development tissue morphogenesis anatomical structure morphogenesis tissue migration anatomical structure development animal organ morphogenesis myotube cell development skeletal muscle fiber development developmental process mesenchyme migration multicellular organismal process mesenchyme morphogenesis animal organ development mesenchyme development tissue development tissue morphogenesis anatomical structure morphogenesis tissue migration anatomical structure development animal organ morphogenesis myotube cell development skeletal muscle fiber development cellular developmental process skeletal muscle tissue development cell development muscle cell differentiation myotube differentiation muscle structure development skeletal muscle organ development muscle cell development cell differentiation cellular process muscle tissue development muscle organ development striated muscle cell differentiation regulation of cellular process cellular biosynthetic process positive regulation of biological process positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process positive regulation of metabolic process organic substance metabolic process positive regulation of biosynthetic process organic substance biosynthetic process positive regulation of macromolecule metabolic process positive regulation of cellular process regulation of biosynthetic process regulation of biological process cellular metabolic process biological regulation regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process positive regulation of gene expression regulation of macromolecule biosynthetic process metabolic process macromolecule metabolic process regulation of metabolic process positive regulation of cellular biosynthetic process cellular component assembly involved in morphogenesis non-membrane-bounded organelle assembly skeletal myofibril assembly cellular component organization cellular component biogenesis striated muscle cell development cellular anatomical entity morphogenesis cellular component assembly actin filament-based process cellular component organization or biogenesis skeletal muscle thin filament assembly actin cytoskeleton organization actomyosin structure organization organelle assembly actin filament organization organelle organization myofibril assembly anatomical structure formation involved in morphogenesis supramolecular fiber organization cytoskeleton organization protein polymerization actin polymerization or depolymerization actin filament polymerization protein-containing complex organization protein-containing complex assembly actin filament bundle organization actin filament bundle assembly	intracellular anatomical structure skeletal muscle myofibril cellular anatomical entity intracellular organelle organelle intracellular non-membrane-bounded organelle contractile muscle fiber cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex non-membrane-bounded organelle cell projection intracellular anatomical structure skeletal muscle myofibril cellular anatomical entity intracellular organelle organelle intracellular non-membrane-bounded organelle contractile muscle fiber cytoplasm supramolecular fiber myofibril supramolecular polymer supramolecular complex non-membrane-bounded organelle cell projection cell leading edge plasma membrane bounded cell projection lamellipodium cytoskeleton actin filament bundle actin cytoskeleton actomyosin stress fiber contractile actin filament bundle polymeric cytoskeletal fiber actin filament protein-containing complex filamentous actin cell body striated muscle thin filament myofilament sarcomere actin-based cell projection filopodium
B	BNI1 protein	small GTPase binding protein binding binding enzyme binding GTPase binding profilin binding cytoskeletal protein binding actin binding identical protein binding	cellular component organization establishment or maintenance of actin cytoskeleton polarity organelle organization establishment or maintenance of cytoskeleton polarity actin cytoskeleton organization actin filament-based process cellular process cellular component organization or biogenesis establishment or maintenance of cell polarity cytoskeleton organization actin filament organization actin nucleation supramolecular fiber organization regulation of cytoskeleton organization cellular component organization establishment or maintenance of actin cytoskeleton polarity organelle organization establishment or maintenance of cytoskeleton polarity actin cytoskeleton organization actin filament-based process cellular process cellular component organization or biogenesis establishment or maintenance of cell polarity cytoskeleton organization actin filament organization actin nucleation supramolecular fiber organization regulation of cytoskeleton organization regulation of biological process regulation of cellular component organization regulation of cellular process regulation of actin filament-based process biological regulation regulation of organelle organization regulation of actin cytoskeleton organization establishment of cell polarity reproductive process in single-celled organism reproductive process budding cell bud growth growth cell division budding cell apical bud growth cell budding asexual reproduction formin-nucleated actin cable assembly cellular component biogenesis parallel actin filament bundle assembly cellular component assembly actin filament bundle organization formin-nucleated actin cable organization actin filament bundle assembly establishment of organelle localization cellular localization microtubule cytoskeleton organization spindle localization mitotic cell cycle localization establishment of mitotic spindle localization establishment of spindle localization establishment of spindle orientation organelle localization establishment of localization in cell cell cycle microtubule cytoskeleton organization involved in mitosis cell cycle process establishment of localization mitotic cell cycle process establishment of mitotic spindle orientation microtubule-based process assembly of actomyosin apparatus involved in cytokinesis mitotic actomyosin contractile ring assembly cytoskeleton-dependent cytokinesis mitotic cytokinetic process actomyosin contractile ring assembly mitotic cytokinesis cortical actin cytoskeleton organization assembly of actomyosin apparatus involved in mitotic cytokinesis actomyosin structure organization cortical cytoskeleton organization cytokinesis cytokinetic process actomyosin contractile ring organization protein localization regulation of localization regulation of protein localization regulation of cellular localization cellular macromolecule localization macromolecule localization regulation of cellular component size negative regulation of biological process protein-containing complex disassembly regulation of protein-containing complex disassembly actin filament depolymerization regulation of actin filament depolymerization actin filament capping negative regulation of supramolecular fiber organization regulation of biological quality negative regulation of actin filament polymerization cellular component disassembly barbed-end actin filament capping negative regulation of cellular component organization negative regulation of protein depolymerization negative regulation of protein-containing complex assembly regulation of protein polymerization regulation of supramolecular fiber organization regulation of protein-containing complex assembly negative regulation of protein-containing complex disassembly negative regulation of cellular process protein-containing complex assembly negative regulation of protein polymerization regulation of actin filament polymerization actin polymerization or depolymerization protein polymerization regulation of actin filament organization actin filament polymerization protein-containing complex organization regulation of protein depolymerization negative regulation of cytoskeleton organization regulation of actin polymerization or depolymerization regulation of cellular component biogenesis negative regulation of organelle organization regulation of actin filament length negative regulation of actin filament depolymerization protein depolymerization regulation of anatomical structure size vesicle localization transport establishment of vesicle localization vesicle targeting vesicle-mediated transport	incipient cellular bud site cellular anatomical entity site of polarized growth cellular bud cellular bud tip cell projection mating projection cell tip mating projection tip cell pole plasma membrane bounded cell projection cell division site cell periphery plasma membrane incipient cellular bud site cellular anatomical entity site of polarized growth cellular bud cellular bud tip cell projection mating projection cell tip mating projection tip cell pole plasma membrane bounded cell projection cell division site cell periphery plasma membrane membrane polymeric cytoskeletal fiber intracellular anatomical structure actin filament actin cytoskeleton intracellular organelle cytoskeleton organelle intracellular non-membrane-bounded organelle supramolecular fiber supramolecular polymer supramolecular complex non-membrane-bounded organelle protein-containing complex polarisome cell cortex cytoplasm ascospore-type prospore prospore membrane intracellular immature spore cellular bud neck

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR020902	Actin/actin-like conserved site	Conserved Site
A	IPR043129	ATPase, nucleotide binding domain	Homologous Superfamily
A	IPR004001	Actin, conserved site	Conserved Site
A	IPR004000	Actin family	Family
B	IPR042201	Formin, FH2 domain superfamily	Homologous Superfamily
B	IPR016024	Armadillo-type fold	Homologous Superfamily
B	IPR015425	Formin, FH2 domain	Domain
B	IPR011989	Armadillo-like helical	Homologous Superfamily
B	IPR014767	Diaphanous autoregulatory (DAD) domain	Domain
B	IPR010472	Formin, FH3 domain	Domain
B	IPR010473	Formin, GTPase-binding domain	Domain
B	IPR014768	Rho GTPase-binding/formin homology 3 (GBD/FH3) domain	Domain

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A	HIC	Parent Component: HIS RESID: AA0317 PSI-MOD : 1'-methyl-L-histidine MOD:00322

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.