1XN1

Crystal Structure Of Lumazine Synthase From Brucella Abortus (Orthorhombic Form At 3.05 Angstroms)

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1xn1a_	Alpha and beta proteins (a/b)	Lumazine synthase	Lumazine synthase	Lumazine synthase	Lumazine synthase	(Brucella abortus ) [TaxId: 235 ],	SCOPe (2.08)
B	d1xn1b_	Alpha and beta proteins (a/b)	Lumazine synthase	Lumazine synthase	Lumazine synthase	Lumazine synthase	(Brucella abortus ) [TaxId: 235 ],	SCOPe (2.08)
C	d1xn1c_	Alpha and beta proteins (a/b)	Lumazine synthase	Lumazine synthase	Lumazine synthase	Lumazine synthase	(Brucella abortus ) [TaxId: 235 ],	SCOPe (2.08)
D	d1xn1d_	Alpha and beta proteins (a/b)	Lumazine synthase	Lumazine synthase	Lumazine synthase	Lumazine synthase	(Brucella abortus ) [TaxId: 235 ],	SCOPe (2.08)
E	d1xn1e_	Alpha and beta proteins (a/b)	Lumazine synthase	Lumazine synthase	Lumazine synthase	Lumazine synthase	(Brucella abortus ) [TaxId: 235 ],	SCOPe (2.08)
F	d1xn1f_	Alpha and beta proteins (a/b)	Lumazine synthase	Lumazine synthase	Lumazine synthase	Lumazine synthase	(Brucella abortus ) [TaxId: 235 ],	SCOPe (2.08)
G	d1xn1g_	Alpha and beta proteins (a/b)	Lumazine synthase	Lumazine synthase	Lumazine synthase	Lumazine synthase	(Brucella abortus ) [TaxId: 235 ],	SCOPe (2.08)
H	d1xn1h_	Alpha and beta proteins (a/b)	Lumazine synthase	Lumazine synthase	Lumazine synthase	Lumazine synthase	(Brucella abortus ) [TaxId: 235 ],	SCOPe (2.08)
I	d1xn1i_	Alpha and beta proteins (a/b)	Lumazine synthase	Lumazine synthase	Lumazine synthase	Lumazine synthase	(Brucella abortus ) [TaxId: 235 ],	SCOPe (2.08)
J	d1xn1j_	Alpha and beta proteins (a/b)	Lumazine synthase	Lumazine synthase	Lumazine synthase	Lumazine synthase	(Brucella abortus ) [TaxId: 235 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Lumazine synthase	8044030	3001085	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Lumazine synthase	8044030	3001085	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Lumazine synthase	8044030	3001085	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Lumazine synthase	8044030	3001085	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Lumazine synthase	8044030	3001085	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Lumazine synthase	8044030	3001085	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Lumazine synthase	8044030	3001085	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Lumazine synthase	8044030	3001085	SCOP2B (2022-06-29)
I	SCOP2B Superfamily	Lumazine synthase	8044030	3001085	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Lumazine synthase	8044030	3001085	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00885	e1xn1A1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Lumazine synthase	F: PF00885	ECOD (1.6)
B	PF00885	e1xn1B1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Lumazine synthase	F: PF00885	ECOD (1.6)
C	PF00885	e1xn1C1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Lumazine synthase	F: PF00885	ECOD (1.6)
D	PF00885	e1xn1D1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Lumazine synthase	F: PF00885	ECOD (1.6)
E	PF00885	e1xn1E1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Lumazine synthase	F: PF00885	ECOD (1.6)
F	PF00885	e1xn1F1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Lumazine synthase	F: PF00885	ECOD (1.6)
G	PF00885	e1xn1G1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Lumazine synthase	F: PF00885	ECOD (1.6)
H	PF00885	e1xn1H1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Lumazine synthase	F: PF00885	ECOD (1.6)
I	PF00885	e1xn1I1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Lumazine synthase	F: PF00885	ECOD (1.6)
J	PF00885	e1xn1J1	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: Lumazine synthase	F: PF00885	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.960	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Lumazine/riboflavin synthase	CATH (4.3.0)
B	3.40.50.960	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Lumazine/riboflavin synthase	CATH (4.3.0)
C	3.40.50.960	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Lumazine/riboflavin synthase	CATH (4.3.0)
D	3.40.50.960	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Lumazine/riboflavin synthase	CATH (4.3.0)
E	3.40.50.960	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Lumazine/riboflavin synthase	CATH (4.3.0)
F	3.40.50.960	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Lumazine/riboflavin synthase	CATH (4.3.0)
G	3.40.50.960	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Lumazine/riboflavin synthase	CATH (4.3.0)
H	3.40.50.960	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Lumazine/riboflavin synthase	CATH (4.3.0)
I	3.40.50.960	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Lumazine/riboflavin synthase	CATH (4.3.0)
J	3.40.50.960	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Lumazine/riboflavin synthase	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H, I, J	PF00885	6,7-dimethyl-8-ribityllumazine synthase (DMRL_synthase)	6,7-dimethyl-8-ribityllumazine synthase	This family includes the beta chain of 6,7-dimethyl-8- ribityllumazine synthase EC:2.5.1.9, an enzyme involved in riboflavin biosynthesis. The family also includes a subfamily of distant archaebacterial proteins that may also have the same function f ...	This family includes the beta chain of 6,7-dimethyl-8- ribityllumazine synthase EC:2.5.1.9, an enzyme involved in riboflavin biosynthesis. The family also includes a subfamily of distant archaebacterial proteins that may also have the same function for example Swiss:O28856. The family contains a number of different subsets including a family of proteins comprising archaeal lumazine and riboflavin synthases, type I lumazine synthases, and the eubacterial type II lumazine synthases [1]. It has been established that lumazine synthase catalyses the penultimate step in the biosynthesis of riboflavin in plants and microorganisms. The type I lumazine synthases area active in pentameric or icosahedral quaternary assemblies, whereas the type II are decameric [2]. Brucella, a bacterial genus that causes brucellosis, and other Rhizobiales have an atypical riboflavin metabolic pathway. Brucella spp code for both a type-I and a type-II lumazine synthase, and it has been shown that at least one of these two has to be present in order for Brucella to be viable, showing that in the case of Brucella flavin metabolism is implicated in bacterial virulence [3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H, I, J	6,7-dimethyl-8-ribityllumazine synthase	6,7-dimethyl-8-ribityllumazine synthase activity transferase activity transferase activity, transferring alkyl or aryl (other than methyl) groups catalytic activity	organonitrogen compound metabolic process water-soluble vitamin biosynthetic process small molecule biosynthetic process cellular biosynthetic process flavin-containing compound metabolic process cellular metabolic process organonitrogen compound biosynthetic process vitamin biosynthetic process riboflavin biosynthetic process vitamin metabolic process organic cyclic compound metabolic process flavin-containing compound biosynthetic process organic substance metabolic process biosynthetic process organic cyclic compound biosynthetic process organonitrogen compound metabolic process water-soluble vitamin biosynthetic process small molecule biosynthetic process cellular biosynthetic process flavin-containing compound metabolic process cellular metabolic process organonitrogen compound biosynthetic process vitamin biosynthetic process riboflavin biosynthetic process vitamin metabolic process organic cyclic compound metabolic process flavin-containing compound biosynthetic process organic substance metabolic process biosynthetic process organic cyclic compound biosynthetic process organic substance biosynthetic process metabolic process small molecule metabolic process cellular process water-soluble vitamin metabolic process riboflavin metabolic process	protein-containing complex catalytic complex riboflavin synthase complex transferase complex intracellular anatomical structure cytoplasm cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H, I, J	IPR036467	Lumazine/riboflavin synthase superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G, H, I, J	IPR034964	Lumazine synthase	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J	IPR002180	Lumazine/riboflavin synthase	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.