1V04

serum paraoxonase by directed evolution

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1v04a_	All beta proteins	6-bladed beta-propeller	Calcium-dependent phosphotriesterase	Serum paraoxonase/arylesterase 1, PON1	Serum paraoxonase/arylesterase 1, PON1	(Homo sapiens ) [TaxId: 9606 ], (Oryctolagus cuniculus ) [TaxId: 9986 ], (Mus musculus ) [TaxId: 10090 ], (Rattus rattus ) [TaxId: 10117 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Serum paraoxonase/arylesterase 1 PON1	8022957	4000928	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Calcium-dependent phosphotriesterase	8035337	3000504	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	SGL	e1v04A1	A: beta duplicates or obligate multimers	X: beta-propeller-like	H: beta-propeller	T: 6-bladed	F: SGL	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.120.10.30	Mainly Beta	6 Propeller	Neuraminidase	TolB, C-terminal domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF01731	Arylesterase (Arylesterase)	Arylesterase	-	Repeat

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	SERUM PARAOXONASE/ARYLESTERASE 1	acyl-L-homoserine-lactone lactonohydrolase activity hydrolase activity, acting on ester bonds carboxylic ester hydrolase activity lactonohydrolase activity hydrolase activity catalytic activity cation binding ion binding binding calcium ion binding metal ion binding small molecule binding arylesterase activity antioxidant activity acyl-L-homoserine-lactone lactonohydrolase activity hydrolase activity, acting on ester bonds carboxylic ester hydrolase activity lactonohydrolase activity hydrolase activity catalytic activity cation binding ion binding binding calcium ion binding metal ion binding small molecule binding arylesterase activity antioxidant activity phosphoric ester hydrolase activity aryldialkylphosphatase activity phosphatase activity	response to organic substance response to stimulus response to chemical response to organophosphorus	protein-containing complex extracellular region plasma lipoprotein particle high-density lipoprotein particle extracellular space cellular anatomical entity lipoprotein particle protein-lipid complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR008363	Paraoxonase1	Family
A	IPR011042	Six-bladed beta-propeller, TolB-like	Homologous Superfamily
A	IPR002640	Arylesterase	Family

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A	OPM	Transmembrane	Bitopic proteins	Calcium-dependent phosphotriesterase	Arylesterase

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	arylesterase M-CSA #740	This variant of serum paraoxonase (PON1) was obtained by directed evolution. Members of the PON family have been identified, and PON1 is present in Homo sapiens, Oryctolagns cuniculus, Mus musculus and Rattus rattus. It catalyses the hydrolysis and thereby inactivation of various organophosphates, including the nerve agents sarin and soman. Recently, PON1 has been found to play an important role in drug metabolism and in the prevention of atherosclerosis.	Defined by 7 residues: GLU:A-53HIS:A-115HIS:A-134ASN:A-168ASN:A-224ASP:A-269ASN:A-270 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 3.1.1.2 (PDB Primary Data) Search M-CSA Motif + EC 3.1.1.2 EC: 3.1.8.1 (PDB Primary Data) Search M-CSA Motif + EC 3.1.8.1

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.