1UN0

Crystal Structure of Yeast Karyopherin (Importin) alpha in complex with a Nup2p N-terminal fragment

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1un0a_	All alpha proteins	alpha-alpha superhelix	ARM repeat	Armadillo repeat	Karyopherin alpha	BAKER'S YEAST (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
B	d1un0b_	All alpha proteins	alpha-alpha superhelix	ARM repeat	Armadillo repeat	Karyopherin alpha	BAKER'S YEAST (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	ARM repeat-like	8043374	3000116	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	ARM repeat-like	8043374	3000116	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Arm	e1un0A1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: Arm	ECOD (1.6)
B	Arm	e1un0B1	A: alpha superhelices	X: Repetitive alpha hairpins	H: ARM repeat (From Topology)	T: ARM repeat	F: Arm	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.25.10.10	Mainly Alpha	Alpha Horseshoe	Leucine-rich Repeat Variant	Leucine-rich Repeat Variant	CATH (4.3.0)
B	1.25.10.10	Mainly Alpha	Alpha Horseshoe	Leucine-rich Repeat Variant	Leucine-rich Repeat Variant	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00514	Armadillo/beta-catenin-like repeat (Arm)	Armadillo/beta-catenin-like repeat	-	Repeat
A, B	PF01749	Importin beta binding domain (IBB)	Importin beta binding domain	-	Repeat
A, B	PF16186	Atypical Arm repeat (Arm_3)	Atypical Arm repeat	-	Repeat
C, D	PF08911	NUP50 (Nucleoporin 50 kDa) (NUP50)	NUP50 (Nucleoporin 50 kDa)	Nucleoporin 50 kDa (NUP50) acts as a cofactor for the importin-alpha:importin-beta heterodimer, which in turn allows for transportation of many nuclear-targeted proteins through nuclear pore complexes. The C terminus of NUP50 binds importin-beta thro ...	Nucleoporin 50 kDa (NUP50) acts as a cofactor for the importin-alpha:importin-beta heterodimer, which in turn allows for transportation of many nuclear-targeted proteins through nuclear pore complexes. The C terminus of NUP50 binds importin-beta through RAN-GTP, the N terminus binds the C terminus of importin-alpha, while a central domain binds importin-beta. NUP50:importin-alpha:importin-beta then binds cargo and can stimulate nuclear import. The N-terminal domain of NUP50 is also able to actively displace nuclear localisation signals from importin-alpha [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	IMPORTIN ALPHA SUBUNIT	protein-containing complex binding binding nuclear import signal receptor activity nucleocytoplasmic carrier activity molecular carrier activity disordered domain specific binding protein binding protein domain specific binding nuclear localization sequence binding signal sequence binding peptide binding	localization cellular localization import into nucleus nucleocytoplasmic transport transport nuclear transport establishment of localization in cell intracellular transport cellular process establishment of localization protein localization establishment of protein localization NLS-bearing protein import into nucleus nitrogen compound transport localization cellular localization import into nucleus nucleocytoplasmic transport transport nuclear transport establishment of localization in cell intracellular transport cellular process establishment of localization protein localization establishment of protein localization NLS-bearing protein import into nucleus nitrogen compound transport cellular macromolecule localization protein import into nucleus organic substance transport protein localization to nucleus establishment of protein localization to organelle protein transport macromolecule localization protein localization to organelle intracellular protein transport proteasome localization protein-containing complex localization establishment of protein localization to membrane protein targeting localization within membrane protein localization to membrane protein targeting to membrane	protein-containing complex nucleocytoplasmic transport complex NLS-dependent protein nuclear import complex membrane-bounded organelle intracellular organelle organelle envelope intracellular anatomical structure organelle nuclear envelope nucleus cellular anatomical entity intracellular membrane-bounded organelle endomembrane system cytoplasm protein-containing complex nucleocytoplasmic transport complex NLS-dependent protein nuclear import complex membrane-bounded organelle intracellular organelle organelle envelope intracellular anatomical structure organelle nuclear envelope nucleus cellular anatomical entity intracellular membrane-bounded organelle endomembrane system cytoplasm cytosol perinuclear region of cytoplasm membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen
C, D	NUCLEOPORIN NUP2	small GTPase binding protein binding binding enzyme binding GTPase binding importin-alpha family protein binding structural molecule activity structural constituent of nuclear pore	protein localization cellular localization establishment of protein localization nitrogen compound transport nucleocytoplasmic transport transport nuclear transport organic substance transport intracellular transport protein export from nucleus establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization protein localization cellular localization establishment of protein localization nitrogen compound transport nucleocytoplasmic transport transport nuclear transport organic substance transport intracellular transport protein export from nucleus establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization cellular process nuclear export intracellular protein transport cellular component organization organelle organization chromosome organization cellular component organization or biogenesis transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery protein localization to nucleus localization within membrane protein localization to membrane protein localization to nuclear envelope protein localization to organelle protein localization to nuclear inner membrane regulation of cellular process cellular biosynthetic process negative regulation of biological process negative regulation of cellular metabolic process protein-DNA complex organization organic substance metabolic process chromatin organization organic substance biosynthetic process negative regulation of cellular process chromatin remodeling negative regulation of macromolecule metabolic process regulation of biosynthetic process regulation of biological process heterochromatin formation protein-containing complex organization epigenetic regulation of gene expression cellular metabolic process negative regulation of metabolic process biological regulation regulation of cellular metabolic process regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process negative regulation of cellular biosynthetic process regulation of cellular biosynthetic process regulation of gene expression negative regulation of gene expression constitutive heterochromatin formation biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process silent mating-type cassette heterochromatin formation negative regulation of gene expression, epigenetic regulation of macromolecule biosynthetic process metabolic process macromolecule metabolic process regulation of metabolic process cellular response to stimulus response to organic substance response to stimulus response to heat mRNA transport response to temperature stimulus cellular response to stress mRNA export from nucleus response to stress RNA localization nucleobase-containing compound transport response to organic cyclic compound cellular response to heat RNA transport nucleic acid transport response to nitrogen compound RNA export from nucleus mRNA export from nucleus in response to heat stress establishment of RNA localization response to chemical response to abiotic stimulus poly(A)+ mRNA export from nucleus NLS-bearing protein import into nucleus import into nucleus protein import into nucleus establishment of protein localization to organelle subtelomeric heterochromatin formation telomere organization post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery	membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle protein-containing complex nucleocytoplasmic transport complex NLS-dependent protein nuclear import complex organelle envelope nuclear envelope nucleus membrane-bounded organelle intracellular organelle intracellular anatomical structure mitochondrion organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle protein-containing complex nucleocytoplasmic transport complex NLS-dependent protein nuclear import complex organelle envelope nuclear envelope nucleus endomembrane system nuclear membrane membrane organelle membrane nuclear protein-containing complex nuclear pore nuclear pore nuclear basket intracellular non-membrane-bounded organelle chromosome chromosome, telomeric region chromosomal region non-membrane-bounded organelle nuclear pore cytoplasmic filaments

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR002652	Importin-alpha, importin-beta-binding domain	Domain
A, B	IPR016024	Armadillo-type fold	Homologous Superfamily
A, B	IPR000225	Armadillo	Repeat
A, B	IPR011989	Armadillo-like helical	Homologous Superfamily
A, B	IPR024931	Importin subunit alpha	Family
A, B	IPR036975	Importin-alpha, importin-beta-binding domain superfamily	Homologous Superfamily
A, B	IPR032413	Atypical Arm repeat	Repeat
C, D	IPR015007	Nuclear pore complex, NUP2/50/61	Domain
C, D	IPR011993	PH-like domain superfamily	Homologous Superfamily
C, D	IPR000156	Ran binding domain	Domain
C, D	IPR045255	Ran binding protein RanBP1-like	Family

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.