1UAQ

The crystal structure of yeast cytosine deaminase

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1uaqa_	Alpha and beta proteins (a/b)	Cytidine deaminase-like	Cytidine deaminase-like	Deoxycytidylate deaminase-like	Cytosine deaminase	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
B	d1uaqb_	Alpha and beta proteins (a/b)	Cytidine deaminase-like	Cytidine deaminase-like	Deoxycytidylate deaminase-like	Cytosine deaminase	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Cytidine deaminase-like	8038289	3001838	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Cytidine deaminase-like	8038289	3001838	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00383	e1uaqA1	A: a+b three layers	X: Cytidine deaminase-like (From Topology)	H: Cytidine deaminase-like (From Topology)	T: Cytidine deaminase-like	F: PF00383	ECOD (1.6)
B	PF00383	e1uaqB1	A: a+b three layers	X: Cytidine deaminase-like (From Topology)	H: Cytidine deaminase-like (From Topology)	T: Cytidine deaminase-like	F: PF00383	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.140.10	Alpha Beta	3-Layer(aba) Sandwich	Cytidine Deaminase	domain 2	CATH (4.3.0)
B	3.40.140.10	Alpha Beta	3-Layer(aba) Sandwich	Cytidine Deaminase	domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00383	Cytidine and deoxycytidylate deaminase zinc-binding region (dCMP_cyt_deam_1)	Cytidine and deoxycytidylate deaminase zinc-binding region	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	cytosine deaminase	cytosine deaminase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines hydrolase activity catalytic activity deaminase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds diaminohydroxyphosphoribosylaminopyrimidine deaminase activity 5-fluorocytosine deaminase activity cation binding transition metal ion binding zinc ion binding ion binding binding metal ion binding cytosine deaminase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines hydrolase activity catalytic activity deaminase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds diaminohydroxyphosphoribosylaminopyrimidine deaminase activity 5-fluorocytosine deaminase activity cation binding transition metal ion binding zinc ion binding ion binding binding metal ion binding small molecule binding	organonitrogen compound metabolic process pyrimidine-containing compound metabolic process cellular metabolic process organonitrogen compound biosynthetic process primary metabolic process nucleobase-containing compound biosynthetic process pyrimidine-containing compound salvage nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process biosynthetic process organic cyclic compound biosynthetic process organic substance biosynthetic process metabolic process organonitrogen compound metabolic process pyrimidine-containing compound metabolic process cellular metabolic process organonitrogen compound biosynthetic process primary metabolic process nucleobase-containing compound biosynthetic process pyrimidine-containing compound salvage nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process biosynthetic process organic cyclic compound biosynthetic process organic substance biosynthetic process metabolic process cellular process pyrimidine-containing compound biosynthetic process cellular metabolic compound salvage pyrimidine nucleobase metabolic process nucleobase-containing small molecule metabolic process nucleobase metabolic process small molecule metabolic process cytosine metabolic process glycosyl compound metabolic process cytidine metabolic process carbohydrate derivative metabolic process pyrimidine nucleoside metabolic process nucleoside metabolic process pyrimidine ribonucleoside metabolic process ribonucleoside metabolic process nucleotide biosynthetic process pyrimidine nucleotide metabolic process ribonucleotide biosynthetic process phosphate-containing compound metabolic process UMP metabolic process phosphorus metabolic process ribose phosphate biosynthetic process nucleoside phosphate metabolic process pyrimidine ribonucleoside monophosphate metabolic process nucleotide metabolic process pyrimidine ribonucleoside monophosphate biosynthetic process organophosphate biosynthetic process nucleoside phosphate biosynthetic process pyrimidine nucleoside monophosphate biosynthetic process ribose phosphate metabolic process pyrimidine ribonucleotide salvage pyrimidine nucleotide salvage nucleotide salvage UMP salvage UMP biosynthetic process ribonucleotide metabolic process ribonucleoside monophosphate metabolic process nucleoside monophosphate metabolic process pyrimidine ribonucleotide metabolic process carbohydrate derivative biosynthetic process pyrimidine nucleoside monophosphate metabolic process nucleoside monophosphate biosynthetic process organophosphate metabolic process ribonucleoside monophosphate biosynthetic process pyrimidine nucleotide biosynthetic process pyrimidine ribonucleotide biosynthetic process	intracellular anatomical structure cytoplasm cellular anatomical entity membrane-bounded organelle intracellular organelle organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR016193	Cytidine deaminase-like	Homologous Superfamily
A, B	IPR002125	Cytidine and deoxycytidylate deaminase domain	Domain
A, B	IPR016192	APOBEC/CMP deaminase, zinc-binding	Binding Site

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	cytosine deaminase (yeast) M-CSA #636	Cytosine deaminase catalyses the hydrolytic deamination of cytosine to uracil. It will also catalyse the deamination of the prodrug 5-fluorocytosine. The is present in bacterial and fungal cells, where it plays an important role in pyrimidine salvage, but not in mammalian cells which use cytidine deaminase instead. The bacterial and yeast cytosine deaminases are have dissimilar folds and catalytic site architectures, and have evolved independently. Yeast cytosine deaminase is however structurally related to the extensively studied bacterial cytidine deaminase. It has a very similar catalytic apparatus and is thought to use the same mechanism.	Defined by 5 residues: HIS:A-62GLU:A-64SER:A-89CYS:A-91CYS:A-94 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 3.5.4.1 (PDB Primary Data) Search M-CSA Motif + EC 3.5.4.1

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.