1U0M

Crystal Structure of 1,3,6,8-Tetrahydroxynaphthalene Synthase (THNS) from Streptomyces coelicolor A3(2): a Bacterial Type III Polyketide Synthase (PKS) Provides Insights into Enzymatic Control of Reactive Polyketide Intermediates

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1u0ma2	Alpha and beta proteins (a/b)	Thiolase-like	Thiolase-like	Chalcone synthase-like	Putative polyketide synthase SCO1206, C-terminal domain	(Streptomyces coelicolor A3(2) ) [TaxId: 100226 ],	SCOPe (2.08)
A	d1u0ma1	Alpha and beta proteins (a/b)	Thiolase-like	Thiolase-like	Chalcone synthase-like	Putative polyketide synthase SCO1206, N-terminal domain	(Streptomyces coelicolor A3(2) ) [TaxId: 100226 ],	SCOPe (2.08)
B	d1u0mb2	Alpha and beta proteins (a/b)	Thiolase-like	Thiolase-like	Chalcone synthase-like	Putative polyketide synthase SCO1206, C-terminal domain	(Streptomyces coelicolor A3(2) ) [TaxId: 100226 ],	SCOPe (2.08)
B	d1u0mb1	Alpha and beta proteins (a/b)	Thiolase-like	Thiolase-like	Chalcone synthase-like	Putative polyketide synthase SCO1206, N-terminal domain	(Streptomyces coelicolor A3(2) ) [TaxId: 100226 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Chalcone synthase-like	8028244	4000864	SCOP2 (2022-06-29)
A	SCOP2 Family	Chalcone synthase-like	8028245	4000864	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Thiolase-like	8040623	3000122	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Thiolase-like	8040624	3000122	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Thiolase-like	8040623	3000122	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Thiolase-like	8040624	3000122	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00195	e1u0mA1	A: a/b three-layered sandwiches	X: Thiolase-like (From Topology)	H: Thiolase-like (From Topology)	T: Thiolase-like	F: PF00195	ECOD (1.6)
A	PF02797	e1u0mA2	A: a/b three-layered sandwiches	X: Thiolase-like (From Topology)	H: Thiolase-like (From Topology)	T: Thiolase-like	F: PF02797	ECOD (1.6)
B	PF00195	e1u0mB1	A: a/b three-layered sandwiches	X: Thiolase-like (From Topology)	H: Thiolase-like (From Topology)	T: Thiolase-like	F: PF00195	ECOD (1.6)
B	PF02797	e1u0mB2	A: a/b three-layered sandwiches	X: Thiolase-like (From Topology)	H: Thiolase-like (From Topology)	T: Thiolase-like	F: PF02797	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.47.10	Alpha Beta	3-Layer(aba) Sandwich	Peroxisomal Thiolase	Chain A, domain 1	CATH (4.3.0)
B	3.40.47.10	Alpha Beta	3-Layer(aba) Sandwich	Peroxisomal Thiolase	Chain A, domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00195	Chalcone and stilbene synthases, N-terminal domain (Chal_sti_synt_N)	Chalcone and stilbene synthases, N-terminal domain	The C-terminal domain of Chalcone synthase is reported to be structurally similar to domains in thiolase and beta-ketoacyl synthase. The differences in activity are accounted for by differences in this N-terminal domain.	Domain
A, B	PF02797	Chalcone and stilbene synthases, C-terminal domain (Chal_sti_synt_C)	Chalcone and stilbene synthases, C-terminal domain	This domain of chalcone synthase is reported to be structurally similar to domains in thiolase and beta-ketoacyl synthase. The differences in activity are accounted for by differences in the N-terminal domain.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	putative polyketide synthase	acyltransferase activity acyltransferase activity, transferring groups other than amino-acyl groups transferase activity catalytic activity	organic substance metabolic process polyketide biosynthetic process secondary metabolite biosynthetic process biosynthetic process polyketide metabolic process organic substance biosynthetic process metabolic process secondary metabolic process pigment metabolic process organic hydroxy compound metabolic process pigment biosynthetic process melanin metabolic process organic cyclic compound metabolic process organic hydroxy compound biosynthetic process organic substance metabolic process polyketide biosynthetic process secondary metabolite biosynthetic process biosynthetic process polyketide metabolic process organic substance biosynthetic process metabolic process secondary metabolic process pigment metabolic process organic hydroxy compound metabolic process pigment biosynthetic process melanin metabolic process organic cyclic compound metabolic process organic hydroxy compound biosynthetic process phenol-containing compound metabolic process organic cyclic compound biosynthetic process phenol-containing compound biosynthetic process melanin biosynthetic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR001099	Chalcone/stilbene synthase, N-terminal	Domain
A, B	IPR016039	Thiolase-like	Homologous Superfamily
A, B	IPR012328	Chalcone/stilbene synthase, C-terminal	Domain
A, B	IPR011141	Polyketide synthase, type III	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.