1T84

Solution structure of the Wiskott-Aldrich Syndrome Protein (WASP) autoinhibited core domain complexed with (S)-wiskostatin, a small molecule inhibitor

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1t84a_	All alpha proteins	Wiscott-Aldrich syndrome protein, WASP, C-terminal domain	Wiscott-Aldrich syndrome protein, WASP, C-terminal domain	Wiscott-Aldrich syndrome protein, WASP, C-terminal domain	Wiscott-Aldrich syndrome protein, WASP, C-terminal domain	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Wiscott-Aldrich syndrome protein WASP C-terminal domain	8037843	3000677	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00786	e1t84A1	A: alpha arrays	X: CRIB domain (From Topology)	H: CRIB domain (From Topology)	T: CRIB domain	F: PF00786	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.90.810.10	Alpha Beta	Alpha-Beta Complex	SerineThreonine-protein kinase PAK-alpha	Chain A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00786	P21-Rho-binding domain (PBD)	P21-Rho-binding domain	Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Wiskott-Aldrich syndrome protein	small GTPase binding protein binding binding enzyme binding GTPase binding phospholipase binding SH3 domain binding protein domain specific binding GTPase regulator activity enzyme regulator activity nucleoside-triphosphatase regulator activity molecular function regulator activity kinase binding protein kinase binding small GTPase binding protein binding binding enzyme binding GTPase binding phospholipase binding SH3 domain binding protein domain specific binding GTPase regulator activity enzyme regulator activity nucleoside-triphosphatase regulator activity molecular function regulator activity kinase binding protein kinase binding cytoskeletal protein binding actin binding identical protein binding	multicellular organismal process regulation of body fluid levels wound healing response to stimulus hemostasis biological regulation blood coagulation response to stress coagulation regulation of biological quality response to wounding cellular component organization cellular component biogenesis cellular component assembly multicellular organismal process regulation of body fluid levels wound healing response to stimulus hemostasis biological regulation blood coagulation response to stress coagulation regulation of biological quality response to wounding cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein-containing complex assembly cellular process cellular component organization or biogenesis regulation of cytoskeleton organization regulation of cellular process regulation of cellular component organization regulation of actin filament-based process regulation of actin filament bundle assembly actin filament bundle organization actin filament-based process stress fiber assembly regulation of biological process regulation of actin filament organization actin cytoskeleton organization actomyosin structure organization actin filament organization regulation of cellular component biogenesis regulation of organelle organization regulation of stress fiber assembly organelle organization contractile actin filament bundle assembly regulation of actomyosin structure organization actin filament bundle assembly regulation of actin cytoskeleton organization regulation of supramolecular fiber organization supramolecular fiber organization cytoskeleton organization immune response immune system process negative regulation of biological process negative regulation of cellular process negative regulation of supramolecular fiber organization negative regulation of stress fiber assembly negative regulation of cellular component organization negative regulation of cytoskeleton organization negative regulation of actin filament bundle assembly negative regulation of organelle organization protein polymerization actin polymerization or depolymerization actin filament polymerization developmental process epidermis development anatomical structure development tissue development negative regulation of locomotion regulation of cell motility regulation of locomotion negative regulation of cell motility cell motility locomotion defense response regulation of cellular component size regulation of actin polymerization or depolymerization regulation of actin filament length regulation of anatomical structure size T cell activation cell activation lymphocyte activation leukocyte activation cellular response to stimulus regulation of DNA recombination regulation of double-strand break repair regulation of cellular response to stress positive regulation of biological process positive regulation of double-strand break repair via homologous recombination regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of response to stimulus positive regulation of metabolic process organic substance metabolic process positive regulation of DNA repair cellular response to stress regulation of response to stress recombinational repair positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process double-strand break repair via homologous recombination positive regulation of cellular process DNA repair positive regulation of DNA recombination double-strand break repair positive regulation of nucleobase-containing compound metabolic process DNA recombination primary metabolic process DNA metabolic process regulation of macromolecule metabolic process nucleic acid metabolic process positive regulation of double-strand break repair regulation of DNA repair regulation of double-strand break repair via homologous recombination organic cyclic compound metabolic process positive regulation of DNA metabolic process DNA damage response regulation of response to stimulus metabolic process macromolecule metabolic process regulation of metabolic process regulation of DNA metabolic process localization cellular localization transport establishment of localization in cell intracellular transport establishment of localization vesicle-mediated transport endosomal transport response to organic substance biological process involved in interspecies interaction between organisms response to type II interferon response to cytokine cellular response to type II interferon response to biotic stimulus innate immune response cellular response to cytokine stimulus response to external biotic stimulus cellular response to organic substance response to other organism defense response to symbiont response to external stimulus cellular response to chemical stimulus response to chemical defense response to other organism small GTPase-mediated signal transduction signaling Cdc42 protein signal transduction Rho protein signal transduction intracellular signal transduction cell communication intracellular signaling cassette signal transduction lamellipodium assembly plasma membrane bounded cell projection organization cell projection organization regulation of cell projection organization regulation of cell projection assembly plasma membrane bounded cell projection assembly regulation of plasma membrane bounded cell projection assembly regulation of lamellipodium assembly regulation of lamellipodium organization lamellipodium organization regulation of plasma membrane bounded cell projection organization cell projection assembly positive regulation of macromolecule biosynthetic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II organic substance biosynthetic process regulation of transcription by RNA polymerase II cellular metabolic process regulation of cellular metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process regulation of gene expression biosynthetic process organic cyclic compound biosynthetic process regulation of macromolecule biosynthetic process regulation of DNA-templated transcription positive regulation of DNA-templated transcription cellular biosynthetic process RNA metabolic process positive regulation of cellular metabolic process RNA biosynthetic process nucleic acid biosynthetic process positive regulation of biosynthetic process regulation of RNA biosynthetic process regulation of biosynthetic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process regulation of cellular biosynthetic process positive regulation of RNA biosynthetic process positive regulation of cellular biosynthetic process adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains lymphocyte mediated immunity regulation of immune effector process T cell mediated immunity T cell antigen processing and presentation antigen processing and presentation regulation of antigen processing and presentation regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains leukocyte mediated immunity regulation of immune system process regulation of T cell mediated immunity regulation of T cell antigen processing and presentation regulation of adaptive immune response adaptive immune response immune effector process regulation of leukocyte mediated immunity regulation of lymphocyte mediated immunity regulation of immune response actin filament-based movement	cell-cell junction anchoring junction cell junction cellular anatomical entity extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle intracellular organelle cell-cell junction anchoring junction cell junction cellular anatomical entity extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle intracellular organelle intracellular anatomical structure cytoskeleton intracellular non-membrane-bounded organelle actin cytoskeleton non-membrane-bounded organelle cytoplasm cytosol intracellular vesicle phagocytic vesicle intracellular membrane-bounded organelle cytoplasmic vesicle endocytic vesicle cell periphery plasma membrane membrane vesicle membrane organelle membrane polymeric cytoskeletal fiber actin filament supramolecular fiber supramolecular polymer supramolecular complex site of double-strand break site of DNA damage chromosome nucleus

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR000095	CRIB domain	Domain
A	IPR000697	WH1/EVH1 domain	Domain
A	IPR036936	CRIB domain superfamily	Homologous Superfamily
A	IPR011993	PH-like domain superfamily	Homologous Superfamily
A	IPR003124	WH2 domain	Domain
A	IPR011026	Actin nucleation-promoting factor WAS, C-terminal	Homologous Superfamily
A	IPR033927	WASP family, EVH1 domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P42768	Wiskott-Aldrich syndrome thrombocytopenia 1 X-linked severe congenital neutropenia severe congenital neutropenia agranulocytosis lymphoma osteosarcoma cutaneous lupus erythematosus malignant mesothelioma psoriasis Thrombocytopenia, X-Linked, Intermittent leukopenia neutropenia

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.