1SZ1

Mechanism of CCA-adding enzymes specificity revealed by crystal structures of ternary complexes

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C [auth A]	d1sz1a1	All alpha proteins	PAP/OAS1 substrate-binding domain	PAP/OAS1 substrate-binding domain	Archaeal tRNA CCA-adding enzyme substrate-binding domain	tRNA nucleotidyltransferase, second domain	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
C [auth A]	d1sz1a3	Alpha and beta proteins (a+b)	Ferredoxin-like	PAP/Archaeal CCA-adding enzyme, C-terminal domain	Archaeal tRNA CCA-adding enzyme	tRNA nucleotidyltransferase, C-terminal domain	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
C [auth A]	d1sz1a2	Alpha and beta proteins (a+b)	Nucleotidyltransferase	Nucleotidyltransferase	Archaeal tRNA CCA-adding enzyme catalytic domain	tRNA nucleotidyltransferase, N-terminal domain	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
D [auth B]	d1sz1b1	All alpha proteins	PAP/OAS1 substrate-binding domain	PAP/OAS1 substrate-binding domain	Archaeal tRNA CCA-adding enzyme substrate-binding domain	tRNA nucleotidyltransferase, second domain	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
D [auth B]	d1sz1b3	Alpha and beta proteins (a+b)	Ferredoxin-like	PAP/Archaeal CCA-adding enzyme, C-terminal domain	Archaeal tRNA CCA-adding enzyme	tRNA nucleotidyltransferase, C-terminal domain	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)
D [auth B]	d1sz1b2	Alpha and beta proteins (a+b)	Nucleotidyltransferase	Nucleotidyltransferase	Archaeal tRNA CCA-adding enzyme catalytic domain	tRNA nucleotidyltransferase, N-terminal domain	(Archaeoglobus fulgidus ) [TaxId: 2234 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C [auth A]	SCOP2B Superfamily	PAP/Archaeal CCA-adding enzyme C-terminal domain-like	8040438	3001147	SCOP2B (2022-06-29)
C [auth A]	SCOP2B Superfamily	Nucleotidyltransferase-like	8040434	3000129	SCOP2B (2022-06-29)
C [auth A]	SCOP2B Superfamily	PAP/OAS1 substrate-binding domain-like	8040432	3000941	SCOP2B (2022-06-29)
D [auth B]	SCOP2B Superfamily	Nucleotidyltransferase-like	8040434	3000129	SCOP2B (2022-06-29)
D [auth B]	SCOP2B Superfamily	PAP/OAS1 substrate-binding domain-like	8040432	3000941	SCOP2B (2022-06-29)
D [auth B]	SCOP2B Superfamily	PAP/Archaeal CCA-adding enzyme C-terminal domain-like	8040438	3001147	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C [auth A]	tRNA_NucTransf2	e1sz1A1	A: alpha arrays	X: HhH/H2TH	H: SAM/DNA-glycosylase	T: PAP/OAS1 substrate-binding domain	F: tRNA_NucTransf2	ECOD (1.6)
C [auth A]	PRK13300	e1sz1A2	A: a+b two layers	X: Alpha-beta plaits	H: PAP/Archaeal CCA-adding enzyme, C-terminal domain (From Topology)	T: PAP/Archaeal CCA-adding enzyme, C-terminal domain	F: PRK13300	ECOD (1.6)
C [auth A]	NTP_transf_2	e1sz1A3	A: a+b three layers	X: Nucleotidyltransferase-like	H: Nucleotidyltransferase (From Topology)	T: Nucleotidyltransferase	F: NTP_transf_2	ECOD (1.6)
D [auth B]	tRNA_NucTransf2	e1sz1B1	A: alpha arrays	X: HhH/H2TH	H: SAM/DNA-glycosylase	T: PAP/OAS1 substrate-binding domain	F: tRNA_NucTransf2	ECOD (1.6)
D [auth B]	PRK13300	e1sz1B2	A: a+b two layers	X: Alpha-beta plaits	H: PAP/Archaeal CCA-adding enzyme, C-terminal domain (From Topology)	T: PAP/Archaeal CCA-adding enzyme, C-terminal domain	F: PRK13300	ECOD (1.6)
D [auth B]	NTP_transf_2	e1sz1B3	A: a+b three layers	X: Nucleotidyltransferase-like	H: Nucleotidyltransferase (From Topology)	T: Nucleotidyltransferase	F: NTP_transf_2	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
C [auth A], D [auth B]	PF21133	Archaeal CCA-adding enzyme, C-terminal domain (CAA_C_arc)	Archaeal CCA-adding enzyme, C-terminal domain	Archaeal CCA-adding enzyme builds and repairs the 3'-end of tRNA [1-4]. This is the C-terminal domain (tail) of CCA-adding enzyme, which serves as a ruler to ensure that only tRNAs and tRNA-like transcripts are substrates for the enzyme and also cont ...	Archaeal CCA-adding enzyme builds and repairs the 3'-end of tRNA [1-4]. This is the C-terminal domain (tail) of CCA-adding enzyme, which serves as a ruler to ensure that only tRNAs and tRNA-like transcripts are substrates for the enzyme and also contributes to the discrimination between stable and unstable RNA substrates [4].	Domain
C [auth A], D [auth B]	PF01909	Nucleotidyltransferase domain (NTP_transf_2)	Nucleotidyltransferase domain	Members of this family belong to a large family of nucleotidyltransferases [1]. This family includes kanamycin nucleotidyltransferase (KNTase) which is a plasmid-coded enzyme responsible for some types of bacterial resistance to aminoglycosides. KNTa ...	Members of this family belong to a large family of nucleotidyltransferases [1]. This family includes kanamycin nucleotidyltransferase (KNTase) which is a plasmid-coded enzyme responsible for some types of bacterial resistance to aminoglycosides. KNTase in-activates antibiotics by catalysing the addition of a nucleotidyl group onto the drug.	Domain
C [auth A], D [auth B]	PF09249	tRNA nucleotidyltransferase, second domain (tRNA_NucTransf2)	tRNA nucleotidyltransferase, second domain	Members of this family adopt a structure consisting of a five helical bundle core. They are predominantly found in Archaeal tRNA nucleotidyltransferase, following the catalytic nucleotidyltransferase domain [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
C [auth A], D [auth B]	tRNA nucleotidyltransferase	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding CCACCA tRNA nucleotidyltransferase activity cytidylyltransferase activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid catalytic activity, acting on a tRNA transferase activity nucleotidyltransferase activity catalytic activity CCA tRNA nucleotidyltransferase activity transferase activity, transferring phosphorus-containing groups RNA binding tRNA binding nucleic acid binding	nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process RNA metabolic process metabolic process primary metabolic process macromolecule metabolic process RNA repair nucleic acid metabolic process catabolic process macromolecule catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process tRNA metabolic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process organic substance metabolic process RNA metabolic process metabolic process primary metabolic process macromolecule metabolic process RNA repair nucleic acid metabolic process catabolic process macromolecule catabolic process organic cyclic compound catabolic process nucleobase-containing compound catabolic process tRNA metabolic process tRNA surveillance ncRNA metabolic process nucleic acid catabolic process RNA surveillance organic substance catabolic process tRNA decay RNA catabolic process cellular biosynthetic process tRNA 3'-terminal CCA addition tRNA processing organic substance biosynthetic process RNA 3'-end processing RNA processing tRNA 3'-end processing cellular metabolic process gene expression macromolecule biosynthetic process biosynthetic process cellular process	-
A [auth E], B [auth F]	T-RNA (76-MER)	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
C [auth A], D [auth B]	IPR011068	Nucleotidyltransferase, class I-like, C-terminal	Homologous Superfamily
C [auth A], D [auth B]	IPR006116	2-5OAS/ClassI-CCAase, nucleotidyltransferase domain	Domain
C [auth A], D [auth B]	IPR048833	CCA-adding enzyme, C-terminal domain	Domain
C [auth A], D [auth B]	IPR042090	tRNA nucleotidyltransferase, domain 2	Homologous Superfamily
C [auth A], D [auth B]	IPR043519	Nucleotidyltransferase superfamily	Homologous Superfamily
C [auth A], D [auth B]	IPR015329	tRNA nucleotidyltransferase, substrate binding	Domain
C [auth A], D [auth B]	IPR002934	Polymerase, nucleotidyl transferase domain	Domain
C [auth A], D [auth B]	IPR008229	tRNA nucleotidyltransferase, archaea	Family

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.