1SEJ

Crystal Structure of Dihydrofolate Reductase-Thymidylate Synthase from Cryptosporidium hominis Bound to 1843U89/NADPH/dUMP

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1seja1	Alpha and beta proteins (a/b)	Dihydrofolate reductase-like	Dihydrofolate reductase-like	Dihydrofolate reductases	Bifunctional enzyme dihydrofolate reductase-thymidylate synthase, DFR domain	(Cryptosporidium hominis ) [TaxId: 237895 ],	SCOPe (2.08)
A	d1seja2	Alpha and beta proteins (a+b)	Thymidylate synthase/dCMP hydroxymethylase	Thymidylate synthase/dCMP hydroxymethylase	Thymidylate synthase/dCMP hydroxymethylase	Bifunctional enzyme dihydrofolate reductase-thymidylate synthase, TS domain	(Cryptosporidium hominis ) [TaxId: 237895 ],	SCOPe (2.08)
B	d1sejb1	Alpha and beta proteins (a/b)	Dihydrofolate reductase-like	Dihydrofolate reductase-like	Dihydrofolate reductases	Bifunctional enzyme dihydrofolate reductase-thymidylate synthase, DFR domain	(Cryptosporidium hominis ) [TaxId: 237895 ],	SCOPe (2.08)
B	d1sejb2	Alpha and beta proteins (a+b)	Thymidylate synthase/dCMP hydroxymethylase	Thymidylate synthase/dCMP hydroxymethylase	Thymidylate synthase/dCMP hydroxymethylase	Bifunctional enzyme dihydrofolate reductase-thymidylate synthase, TS domain	(Cryptosporidium hominis ) [TaxId: 237895 ],	SCOPe (2.08)
C	d1sejc1	Alpha and beta proteins (a/b)	Dihydrofolate reductase-like	Dihydrofolate reductase-like	Dihydrofolate reductases	Bifunctional enzyme dihydrofolate reductase-thymidylate synthase, DFR domain	(Cryptosporidium hominis ) [TaxId: 237895 ],	SCOPe (2.08)
C	d1sejc2	Alpha and beta proteins (a+b)	Thymidylate synthase/dCMP hydroxymethylase	Thymidylate synthase/dCMP hydroxymethylase	Thymidylate synthase/dCMP hydroxymethylase	Bifunctional enzyme dihydrofolate reductase-thymidylate synthase, TS domain	(Cryptosporidium hominis ) [TaxId: 237895 ],	SCOPe (2.08)
D	d1sejd1	Alpha and beta proteins (a/b)	Dihydrofolate reductase-like	Dihydrofolate reductase-like	Dihydrofolate reductases	Bifunctional enzyme dihydrofolate reductase-thymidylate synthase, DFR domain	(Cryptosporidium hominis ) [TaxId: 237895 ],	SCOPe (2.08)
D	d1sejd2	Alpha and beta proteins (a+b)	Thymidylate synthase/dCMP hydroxymethylase	Thymidylate synthase/dCMP hydroxymethylase	Thymidylate synthase/dCMP hydroxymethylase	Bifunctional enzyme dihydrofolate reductase-thymidylate synthase, TS domain	(Cryptosporidium hominis ) [TaxId: 237895 ],	SCOPe (2.08)
E	d1seje1	Alpha and beta proteins (a/b)	Dihydrofolate reductase-like	Dihydrofolate reductase-like	Dihydrofolate reductases	Bifunctional enzyme dihydrofolate reductase-thymidylate synthase, DFR domain	(Cryptosporidium hominis ) [TaxId: 237895 ],	SCOPe (2.08)
E	d1seje2	Alpha and beta proteins (a+b)	Thymidylate synthase/dCMP hydroxymethylase	Thymidylate synthase/dCMP hydroxymethylase	Thymidylate synthase/dCMP hydroxymethylase	Bifunctional enzyme dihydrofolate reductase-thymidylate synthase, TS domain	(Cryptosporidium hominis ) [TaxId: 237895 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Dihydrofolate reductases	8026994	4000755	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Dihydrofolate reductase-like	8039373	3001255	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Dihydrofolate reductase-like	8039373	3001255	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Dihydrofolate reductase-like	8039373	3001255	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Dihydrofolate reductase-like	8039373	3001255	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Dihydrofolate reductase-like	8039373	3001255	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00303	e1sejA1	A: a+b two layers	X: Thymidylate synthase/dCMP hydroxymethylase (From Topology)	H: Thymidylate synthase/dCMP hydroxymethylase (From Topology)	T: Thymidylate synthase/dCMP hydroxymethylase	F: PF00303	ECOD (1.6)
A	PF00186	e1sejA2	A: a/b three-layered sandwiches	X: Dihydrofolate reductases (From Topology)	H: Dihydrofolate reductases (From Topology)	T: Dihydrofolate reductases	F: PF00186	ECOD (1.6)
B	PF00303	e1sejB1	A: a+b two layers	X: Thymidylate synthase/dCMP hydroxymethylase (From Topology)	H: Thymidylate synthase/dCMP hydroxymethylase (From Topology)	T: Thymidylate synthase/dCMP hydroxymethylase	F: PF00303	ECOD (1.6)
B	PF00186	e1sejB2	A: a/b three-layered sandwiches	X: Dihydrofolate reductases (From Topology)	H: Dihydrofolate reductases (From Topology)	T: Dihydrofolate reductases	F: PF00186	ECOD (1.6)
C	PF00303	e1sejC2	A: a+b two layers	X: Thymidylate synthase/dCMP hydroxymethylase (From Topology)	H: Thymidylate synthase/dCMP hydroxymethylase (From Topology)	T: Thymidylate synthase/dCMP hydroxymethylase	F: PF00303	ECOD (1.6)
C	PF00186	e1sejC3	A: a/b three-layered sandwiches	X: Dihydrofolate reductases (From Topology)	H: Dihydrofolate reductases (From Topology)	T: Dihydrofolate reductases	F: PF00186	ECOD (1.6)
D	PF00303	e1sejD2	A: a+b two layers	X: Thymidylate synthase/dCMP hydroxymethylase (From Topology)	H: Thymidylate synthase/dCMP hydroxymethylase (From Topology)	T: Thymidylate synthase/dCMP hydroxymethylase	F: PF00303	ECOD (1.6)
D	PF00186	e1sejD3	A: a/b three-layered sandwiches	X: Dihydrofolate reductases (From Topology)	H: Dihydrofolate reductases (From Topology)	T: Dihydrofolate reductases	F: PF00186	ECOD (1.6)
E	PF00303	e1sejE2	A: a+b two layers	X: Thymidylate synthase/dCMP hydroxymethylase (From Topology)	H: Thymidylate synthase/dCMP hydroxymethylase (From Topology)	T: Thymidylate synthase/dCMP hydroxymethylase	F: PF00303	ECOD (1.6)
E	PF00186	e1sejE3	A: a/b three-layered sandwiches	X: Dihydrofolate reductases (From Topology)	H: Dihydrofolate reductases (From Topology)	T: Dihydrofolate reductases	F: PF00186	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.430.10	Alpha Beta	3-Layer(aba) Sandwich	Dihydrofolate Reductase, subunit A	Dihydrofolate Reductase, subunit A	CATH (4.3.0)
A	3.30.572.10	Alpha Beta	2-Layer Sandwich	Thymidylate Synthase	Chain A	CATH (4.3.0)
B	3.40.430.10	Alpha Beta	3-Layer(aba) Sandwich	Dihydrofolate Reductase, subunit A	Dihydrofolate Reductase, subunit A	CATH (4.3.0)
B	3.30.572.10	Alpha Beta	2-Layer Sandwich	Thymidylate Synthase	Chain A	CATH (4.3.0)
C	3.40.430.10	Alpha Beta	3-Layer(aba) Sandwich	Dihydrofolate Reductase, subunit A	Dihydrofolate Reductase, subunit A	CATH (4.3.0)
C	3.30.572.10	Alpha Beta	2-Layer Sandwich	Thymidylate Synthase	Chain A	CATH (4.3.0)
D	3.40.430.10	Alpha Beta	3-Layer(aba) Sandwich	Dihydrofolate Reductase, subunit A	Dihydrofolate Reductase, subunit A	CATH (4.3.0)
D	3.30.572.10	Alpha Beta	2-Layer Sandwich	Thymidylate Synthase	Chain A	CATH (4.3.0)
E	3.40.430.10	Alpha Beta	3-Layer(aba) Sandwich	Dihydrofolate Reductase, subunit A	Dihydrofolate Reductase, subunit A	CATH (4.3.0)
E	3.30.572.10	Alpha Beta	2-Layer Sandwich	Thymidylate Synthase	Chain A	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E	PF00303	Thymidylate synthase (Thymidylat_synt)	Thymidylate synthase	This is a family of proteins that are flavin-dependent thymidylate synthases.	Domain
A, B, C, D, E	PF00186	Dihydrofolate reductase (DHFR_1)	Dihydrofolate reductase	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E	bifunctional dihydrofolate reductase-thymidylate synthase	transferase activity, transferring one-carbon groups transferase activity methyltransferase activity thymidylate synthase activity 5,10-methylenetetrahydrofolate-dependent methyltransferase activity catalytic activity binding nucleoside phosphate binding organic cyclic compound binding nucleotide binding heterocyclic compound binding small molecule binding dihydrofolate reductase activity oxidoreductase activity transferase activity, transferring one-carbon groups transferase activity methyltransferase activity thymidylate synthase activity 5,10-methylenetetrahydrofolate-dependent methyltransferase activity catalytic activity binding nucleoside phosphate binding organic cyclic compound binding nucleotide binding heterocyclic compound binding small molecule binding dihydrofolate reductase activity oxidoreductase activity oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on the CH-NH group of donors	cellular metabolic process small molecule metabolic process metabolic process cellular process one-carbon metabolic process pyrimidine deoxyribonucleotide metabolic process nucleotide biosynthetic process organonitrogen compound biosynthetic process carbohydrate derivative metabolic process pyrimidine nucleotide metabolic process nucleobase-containing compound metabolic process deoxyribonucleotide biosynthetic process pyrimidine deoxyribonucleotide biosynthetic process dTMP metabolic process cellular metabolic process small molecule metabolic process metabolic process cellular process one-carbon metabolic process pyrimidine deoxyribonucleotide metabolic process nucleotide biosynthetic process organonitrogen compound biosynthetic process carbohydrate derivative metabolic process pyrimidine nucleotide metabolic process nucleobase-containing compound metabolic process deoxyribonucleotide biosynthetic process pyrimidine deoxyribonucleotide biosynthetic process dTMP metabolic process phosphate-containing compound metabolic process organic substance biosynthetic process dTMP biosynthetic process phosphorus metabolic process nucleoside phosphate metabolic process pyrimidine-containing compound metabolic process 2'-deoxyribonucleotide biosynthetic process primary metabolic process nucleotide metabolic process deoxyribonucleoside monophosphate metabolic process organic cyclic compound metabolic process biosynthetic process organophosphate biosynthetic process organic cyclic compound biosynthetic process nucleoside phosphate biosynthetic process pyrimidine nucleoside monophosphate biosynthetic process deoxyribose phosphate biosynthetic process pyrimidine deoxyribonucleoside monophosphate metabolic process organic substance metabolic process deoxyribonucleotide metabolic process nucleobase-containing small molecule metabolic process deoxyribonucleoside monophosphate biosynthetic process pyrimidine deoxyribonucleoside monophosphate biosynthetic process pyrimidine-containing compound biosynthetic process nucleoside monophosphate metabolic process carbohydrate derivative biosynthetic process pyrimidine nucleoside monophosphate metabolic process organonitrogen compound metabolic process 2'-deoxyribonucleotide metabolic process deoxyribose phosphate metabolic process nucleobase-containing compound biosynthetic process nucleoside monophosphate biosynthetic process organophosphate metabolic process pyrimidine nucleotide biosynthetic process methylation folic acid-containing compound biosynthetic process cellular modified amino acid metabolic process cellular modified amino acid biosynthetic process folic acid-containing compound metabolic process pteridine-containing compound biosynthetic process tetrahydrofolate biosynthetic process pteridine-containing compound metabolic process tetrahydrofolate metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E	IPR017925	Dihydrofolate reductase conserved site	Conserved Site
A, B, C, D, E	IPR036926	Thymidylate synthase/dCMP hydroxymethylase superfamily	Homologous Superfamily
A, B, C, D, E	IPR012262	Bifunctional dihydrofolate reductase/thymidylate synthase	Family
A, B, C, D, E	IPR000398	Thymidylate synthase	Family
A, B, C, D, E	IPR024072	Dihydrofolate reductase-like domain superfamily	Homologous Superfamily
A, B, C, D, E	IPR001796	Dihydrofolate reductase domain	Domain
A, B, C, D, E	IPR045097	Thymidylate synthase/dCMP hydroxymethylase	Family
A, B, C, D, E	IPR020940	Thymidylate synthase, active site	Active Site
A, B, C, D, E	IPR023451	Thymidylate synthase/dCMP hydroxymethylase domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.