1S3I

Crystal structure of the N terminal hydrolase domain of 10-formyltetrahydrofolate dehydrogenase

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1s3ia1	All beta proteins	FMT C-terminal domain-like	FMT C-terminal domain-like	Post formyltransferase domain	10-formyltetrahydrofolate dehydrogenase domain 2	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)
A	d1s3ia2	Alpha and beta proteins (a/b)	Formyltransferase	Formyltransferase	Formyltransferase	10-formyltetrahydrofolate dehydrogenase domain 1	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Methionyl-tRNA formyltransferase-like	8002492	4000133	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	FMT C-terminal domain-like	8057644	3000015	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Formyltransferase	8057643	3000030	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF02911	e1s3iA1	A: beta barrels	X: FMT C-terminal domain-like	H: FMT C-terminal domain-related (From Topology)	T: FMT C-terminal domain-related	F: PF02911	ECOD (1.6)
A	PF00551	e1s3iA2	A: a/b three-layered sandwiches	X: Formyltransferase (From Topology)	H: Formyltransferase (From Topology)	T: Formyltransferase	F: PF00551	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.170	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Formyl transferase, N-terminal domain	CATH (4.3.0)
A	3.10.25.10	Alpha Beta	Roll	Methionyl-tRNA Fmet Formyltransferase	Chain A, domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF02911	Formyl transferase, C-terminal domain (Formyl_trans_C)	Formyl transferase, C-terminal domain	-	Domain
A	PF00551	Formyl transferase (Formyl_trans_N)	Formyl transferase	This family includes the following members. Glycinamide ribonucleotide transformylase catalyses the third step in de novo purine biosynthesis, the transfer of a formyl group to 5'-phosphoribosylglycinamide. Formyltetrahydrofolate deformylase produces ...	This family includes the following members. Glycinamide ribonucleotide transformylase catalyses the third step in de novo purine biosynthesis, the transfer of a formyl group to 5'-phosphoribosylglycinamide. Formyltetrahydrofolate deformylase produces formate from formyl- tetrahydrofolate. Methionyl-tRNA formyltransferase transfers a formyl group onto the amino terminus of the acyl moiety of the methionyl aminoacyl-tRNA. Inclusion of the following members is supported by PSI-blast. HOXX_BRAJA (P31907) contains a related domain of unknown function. PRTH_PORGI (P46071) contains a related domain of unknown function. Y09P_MYCTU (Q50721) contains a related domain of unknown function.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	10-formyltetrahydrofolate dehydrogenase	oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors aldehyde dehydrogenase (NADP+) activity catalytic activity aldehyde dehydrogenase [NAD(P)+] activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor protein-containing complex binding binding formyltetrahydrofolate dehydrogenase activity oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor oxidoreductase activity, acting on the CH-NH group of donors aldehyde dehydrogenase (NAD+) activity	cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation cellular process bile acid signaling pathway signal transduction cell communication nucleobase-containing compound metabolic process purine nucleotide metabolic process organic substance metabolic process nucleobase-containing small molecule metabolic process cellular response to stimulus regulation of biological process regulation of cellular process response to stimulus signaling biological regulation cellular process bile acid signaling pathway signal transduction cell communication nucleobase-containing compound metabolic process purine nucleotide metabolic process organic substance metabolic process nucleobase-containing small molecule metabolic process phosphate-containing compound metabolic process pyridine-containing compound metabolic process small molecule metabolic process phosphorus metabolic process pyridine nucleotide metabolic process nucleoside phosphate metabolic process nicotinamide nucleotide metabolic process organonitrogen compound metabolic process cellular metabolic process primary metabolic process generation of precursor metabolites and energy nucleotide metabolic process purine-containing compound metabolic process NADP metabolic process organic cyclic compound metabolic process NADPH regeneration organophosphate metabolic process metabolic process catabolic process oxoacid metabolic process dicarboxylic acid catabolic process organic acid metabolic process organic acid catabolic process cellular catabolic process carboxylic acid catabolic process pteridine-containing compound catabolic process 10-formyltetrahydrofolate catabolic process organonitrogen compound catabolic process 10-formyltetrahydrofolate metabolic process dicarboxylic acid metabolic process carboxylic acid metabolic process cellular modified amino acid metabolic process organic cyclic compound catabolic process folic acid-containing compound catabolic process folic acid-containing compound metabolic process small molecule catabolic process pteridine-containing compound metabolic process tetrahydrofolate metabolic process organic substance catabolic process cellular modified amino acid catabolic process one-carbon metabolic process vitamin metabolic process amide metabolic process water-soluble vitamin metabolic process folic acid metabolic process folic acid-containing compound biosynthetic process organonitrogen compound biosynthetic process biosynthetic process cellular modified amino acid biosynthetic process organic cyclic compound biosynthetic process pteridine-containing compound biosynthetic process organic substance biosynthetic process tetrahydrofolate biosynthetic process	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol protein-containing complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR015590	Aldehyde dehydrogenase domain	Domain
A	IPR016161	Aldehyde/histidinol dehydrogenase	Homologous Superfamily
A	IPR036477	Formyl transferase, N-terminal domain superfamily	Homologous Superfamily
A	IPR037022	Formyl transferase, C-terminal domain superfamily	Homologous Superfamily
A	IPR001555	Phosphoribosylglycinamide formyltransferase, active site	Active Site
A	IPR036736	ACP-like superfamily	Homologous Superfamily
A	IPR029510	Aldehyde dehydrogenase, glutamic acid active site	Conserved Site
A	IPR002376	Formyl transferase, N-terminal	Domain
A	IPR005793	Formyl transferase, C-terminal	Domain
A	IPR016160	Aldehyde dehydrogenase, cysteine active site	Conserved Site
A	IPR011034	Formyl transferase-like, C-terminal domain superfamily	Homologous Superfamily
A	IPR009081	Phosphopantetheine binding ACP domain	Domain
A	IPR016162	Aldehyde dehydrogenase, N-terminal	Homologous Superfamily
A	IPR011407	10-formyltetrahydrofolate dehydrogenase	Family
A	IPR016163	Aldehyde dehydrogenase, C-terminal	Homologous Superfamily

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	formyltetrahydrofolate dehydrogenase M-CSA #766	10-Formyltetrahydrofolate dehydrogenase (FDH) converts 10-formyltetrahydrofolate, a precursor for nucleotide biosynthesis, to tetrahydrofolate. FDH comprises two domains: a hydrolase domain that hydrolyses the formyl group off the substrate, and a dehydrogenase domain that reduces the formyl group to carbon dioxide. FDH is an component of a folate metabolism pathway, with a possible role in control of folate levels and cross-talk between purine and thymidine synthesis pathways. Pathogenically, studies imply that FDH may possess a growth-regulatory function and that cancer cells lower FDH expression to enhance proliferation.	Defined by 2 residues: HIS:A-106ASP:A-142 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 1.5.1.6 (PDB Primary Data) Search M-CSA Motif + EC 1.5.1.6

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.