1S3A

NMR Solution Structure of Subunit B8 from Human NADH-Ubiquinone Oxidoreductase Complex I (CI-B8)

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1s3aa1	Alpha and beta proteins (a/b)	Thioredoxin fold	Thioredoxin-like	Mitochondrial ribosomal protein L51/S25/CI-B8 domain	NADH-ubiquinone oxidoreductase b8 subunit, CI-B8	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Mitochondrial ribosomal protein L51/S25/CI-B8 domain	8019958	4002951	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Thioredoxin-like	8032338	3000031	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF05047	e1s3aA1	A: a+b three layers	X: Thioredoxin-like	H: Thioredoxin-like (From Topology)	T: Thioredoxin-like	F: PF05047	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.30.10	Alpha Beta	3-Layer(aba) Sandwich	Glutaredoxin	Glutaredoxin	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF05047	Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain (L51_S25_CI-B8)	Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain	The proteins in this family are located in the mitochondrion. The family includes ribosomal protein L51, and S25. This family also includes mitochondrial NADH-ubiquinone oxidoreductase B8 subunit (CI-B8) EC:1.6.5.3. It is not known whether all member ...	The proteins in this family are located in the mitochondrion. The family includes ribosomal protein L51, and S25. This family also includes mitochondrial NADH-ubiquinone oxidoreductase B8 subunit (CI-B8) EC:1.6.5.3. It is not known whether all members of this family form part of the NADH-ubiquinone oxidoreductase and whether they are also all ribosomal proteins. Structurally related to thioredoxin-fold.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	NADH-ubiquinone oxidoreductase B8 subunit	electron transfer activity NADH dehydrogenase (ubiquinone) activity oxidoreduction-driven active transmembrane transporter activity active transmembrane transporter activity primary active transmembrane transporter activity transporter activity transmembrane transporter activity	protein-containing complex organization NADH dehydrogenase complex assembly cellular component organization cellular component biogenesis organelle organization cellular component assembly mitochondrial respiratory chain complex assembly protein-containing complex assembly cellular process cellular component organization or biogenesis mitochondrial respiratory chain complex I assembly mitochondrion organization aerobic respiration oxidative phosphorylation protein-containing complex organization NADH dehydrogenase complex assembly cellular component organization cellular component biogenesis organelle organization cellular component assembly mitochondrial respiratory chain complex assembly protein-containing complex assembly cellular process cellular component organization or biogenesis mitochondrial respiratory chain complex I assembly mitochondrion organization aerobic respiration oxidative phosphorylation nucleotide biosynthetic process organonitrogen compound biosynthetic process carbohydrate derivative metabolic process purine ribonucleotide biosynthetic process purine ribonucleoside triphosphate biosynthetic process nucleobase-containing compound metabolic process ribonucleotide biosynthetic process purine nucleoside triphosphate biosynthetic process phosphate-containing compound metabolic process organic substance biosynthetic process small molecule metabolic process phosphorus metabolic process ribose phosphate biosynthetic process nucleoside phosphate metabolic process cellular metabolic process primary metabolic process nucleotide metabolic process ribonucleoside triphosphate biosynthetic process organic cyclic compound metabolic process biosynthetic process organophosphate biosynthetic process organic cyclic compound biosynthetic process nucleoside phosphate biosynthetic process proton motive force-driven mitochondrial ATP synthesis metabolic process nucleoside triphosphate metabolic process purine ribonucleotide metabolic process purine nucleotide biosynthetic process ribose phosphate metabolic process cellular respiration ATP metabolic process energy derivation by oxidation of organic compounds ATP biosynthetic process proton motive force-driven ATP synthesis purine nucleotide metabolic process organic substance metabolic process ribonucleotide metabolic process nucleobase-containing small molecule metabolic process purine-containing compound biosynthetic process purine ribonucleoside triphosphate metabolic process ribonucleoside triphosphate metabolic process carbohydrate derivative biosynthetic process purine nucleoside triphosphate metabolic process organonitrogen compound metabolic process nucleoside triphosphate biosynthetic process generation of precursor metabolites and energy nucleobase-containing compound biosynthetic process purine-containing compound metabolic process organophosphate metabolic process developmental process multicellular organismal process chordate embryonic development embryo development in utero embryonic development multicellular organism development hatching embryo development ending in birth or egg hatching blastocyst hatching anatomical structure development organism emergence from protective structure blastocyst development aerobic electron transport chain ATP synthesis coupled electron transport electron transport chain mitochondrial electron transport, NADH to ubiquinone respiratory electron transport chain mitochondrial ATP synthesis coupled electron transport	membrane-bounded organelle mitochondrial membrane intracellular anatomical structure organelle inner membrane mitochondrion membrane cellular anatomical entity intracellular membrane-bounded organelle mitochondrial inner membrane intracellular organelle organelle envelope mitochondrial envelope organelle cytoplasm membrane-bounded organelle mitochondrial membrane intracellular anatomical structure organelle inner membrane mitochondrion membrane cellular anatomical entity intracellular membrane-bounded organelle mitochondrial inner membrane intracellular organelle organelle envelope mitochondrial envelope organelle cytoplasm organelle membrane respiratory chain complex mitochondrial respiratory chain complex I protein-containing complex mitochondrial respirasome membrane protein complex transporter complex NADH dehydrogenase complex oxidoreductase complex mitochondrial protein-containing complex catalytic complex inner mitochondrial membrane protein complex transmembrane transporter complex respiratory chain complex I respirasome

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR007741	Ribosomal protein/NADH dehydrogenase domain	Domain
A	IPR036249	Thioredoxin-like superfamily	Homologous Superfamily
A	IPR016464	NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: O43678	Leigh syndrome plasma cell myeloma type 2 diabetes mellitus cystic leukoencephalopathy without megalencephaly Disease Progression hereditary diffuse gastric adenocarcinoma leukodystrophy mitochondrial complex I deficiency osteosarcoma psoriasis diabetes mellitus gastric cancer inborn errors of metabolism ovarian cancer gastric neoplasm Leigh syndrome plasma cell myeloma type 2 diabetes mellitus cystic leukoencephalopathy without megalencephaly Disease Progression hereditary diffuse gastric adenocarcinoma leukodystrophy mitochondrial complex I deficiency osteosarcoma psoriasis diabetes mellitus gastric cancer inborn errors of metabolism ovarian cancer gastric neoplasm oligodendroglioma

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.