1R8Q

FULL-LENGTH ARF1-GDP-MG IN COMPLEX WITH BREFELDIN A AND A SEC7 DOMAIN

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1r8qa_	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	ADP-ribosylation factor	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
C [auth B]	d1r8qb_	Alpha and beta proteins (a/b)	P-loop containing nucleoside triphosphate hydrolases	P-loop containing nucleoside triphosphate hydrolases	G proteins	ADP-ribosylation factor	cattle (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
B [auth E]	d1r8qe_	All alpha proteins	alpha-alpha superhelix	Sec7 domain	Sec7 domain	Exchange factor ARNO	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
D [auth F]	d1r8qf_	All alpha proteins	alpha-alpha superhelix	Sec7 domain	Sec7 domain	Exchange factor ARNO	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ras-like P-loop GTPases	8056739	3002022	SCOP2B (2022-06-29)
C [auth B]	SCOP2B Superfamily	Ras-like P-loop GTPases	8056739	3002022	SCOP2B (2022-06-29)
B [auth E]	SCOP2B Superfamily	Sec7 domain-like	8040453	3001279	SCOP2B (2022-06-29)
D [auth F]	SCOP2B Superfamily	Sec7 domain-like	8040453	3001279	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Ras	e1r8qA1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
C [auth B]	Ras	e1r8qB1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
B [auth E]	Sec7	e1r8qE1	A: alpha arrays	X: Sec7 domain (From Topology)	H: Sec7 domain (From Topology)	T: Sec7 domain	F: Sec7	ECOD (1.6)
D [auth F]	Sec7	e1r8qF1	A: alpha arrays	X: Sec7 domain (From Topology)	H: Sec7 domain (From Topology)	T: Sec7 domain	F: Sec7	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
C [auth B]	3.40.50.300	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	P-loop containing nucleotide triphosphate hydrolases	CATH (4.3.0)
B [auth E]	1.10.220.20	Mainly Alpha	Orthogonal Bundle	Annexin V	domain 1	CATH (4.3.0)
B [auth E]	1.10.1000.11	Mainly Alpha	Orthogonal Bundle	Arf Nucleotide-binding Site Opener	domain 2	CATH (4.3.0)
D [auth F]	1.10.220.20	Mainly Alpha	Orthogonal Bundle	Annexin V	domain 1	CATH (4.3.0)
D [auth F]	1.10.1000.11	Mainly Alpha	Orthogonal Bundle	Arf Nucleotide-binding Site Opener	domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C [auth B]	PF00025	ADP-ribosylation factor family (Arf)	ADP-ribosylation factor family	Pfam combines a number of different Prosite families together	Domain
B [auth E], D [auth F]	PF01369	Sec7 domain (Sec7)	Sec7 domain	The Sec7 domain is a guanine-nucleotide-exchange-factor (GEF) for the Pfam:PF00025 family [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C [auth B]	ADP-ribosylation factor 1	binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding pyrophosphatase activity GTPase activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity	protein localization cellular localization establishment of protein localization nitrogen compound transport transport organic substance transport intracellular transport establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization cellular process protein localization cellular localization establishment of protein localization nitrogen compound transport transport organic substance transport intracellular transport establishment of localization localization protein transport establishment of localization in cell cellular macromolecule localization macromolecule localization cellular process intracellular protein transport cellular component organization cell junction organization dendritic spine organization postsynapse organization plasma membrane bounded cell projection organization cell projection organization neuron projection organization synapse organization cellular component organization or biogenesis vesicle-mediated transport regulation of cellular process negative regulation of signaling signaling modulation of chemical synaptic transmission negative regulation of biological process negative regulation of synaptic transmission negative regulation of cell communication cell communication synaptic signaling negative regulation of cellular process regulation of biological quality regulation of synaptic plasticity trans-synaptic signaling regulation of signaling regulation of biological process biological regulation cell-cell signaling chemical synaptic transmission regulation of trans-synaptic signaling long-term synaptic depression anterograde trans-synaptic signaling regulation of cell communication regulation of localization import into cell regulation of cellular component organization receptor internalization regulation of transport regulation of receptor-mediated endocytosis regulation of vesicle-mediated transport regulation of receptor internalization endocytosis receptor-mediated endocytosis regulation of endocytosis regulation of Arp2/3 complex-mediated actin nucleation regulation of cytoskeleton organization regulation of actin filament-based process regulation of actin nucleation actin filament-based process regulation of actin filament organization actin cytoskeleton organization actin filament organization Arp2/3 complex-mediated actin nucleation regulation of organelle organization organelle organization regulation of actin cytoskeleton organization regulation of supramolecular fiber organization actin nucleation supramolecular fiber organization cytoskeleton organization	membrane-bounded organelle intracellular organelle intracellular anatomical structure Golgi apparatus organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle endomembrane system Golgi membrane membrane bounding membrane of organelle organelle membrane cytosol membrane-bounded organelle intracellular organelle intracellular anatomical structure Golgi apparatus organelle cytoplasm cellular anatomical entity intracellular membrane-bounded organelle endomembrane system Golgi membrane membrane bounding membrane of organelle organelle membrane cytosol postsynaptic density postsynapse neuron to neuron synapse postsynaptic specialization synapse cell junction asymmetric synapse cell periphery plasma membrane cell projection neuron projection plasma membrane bounded cell projection
B [auth E], D [auth F]	Arno	binding lipid binding GTPase regulator activity enzyme regulator activity guanyl-nucleotide exchange factor activity nucleoside-triphosphatase regulator activity molecular function regulator activity ion binding alcohol binding inositol 1,4,5 trisphosphate binding anion binding small molecule binding	cellular response to stimulus regulation of biological process regulation of signaling small GTPase-mediated signal transduction regulation of cellular process response to stimulus signaling regulation of small GTPase mediated signal transduction biological regulation ARF protein signal transduction regulation of signal transduction intracellular signal transduction cell communication regulation of intracellular signal transduction regulation of response to stimulus cellular response to stimulus regulation of biological process regulation of signaling small GTPase-mediated signal transduction regulation of cellular process response to stimulus signaling regulation of small GTPase mediated signal transduction biological regulation ARF protein signal transduction regulation of signal transduction intracellular signal transduction cell communication regulation of intracellular signal transduction regulation of response to stimulus regulation of ARF protein signal transduction intracellular signaling cassette regulation of cell communication cellular process signal transduction localization import into cell transport endocytosis establishment of localization vesicle-mediated transport cellular component organization organelle organization actin cytoskeleton organization actin filament-based process cellular component organization or biogenesis cytoskeleton organization	cell-cell junction adherens junction anchoring junction cell junction cellular anatomical entity membrane membrane-bounded organelle Golgi membrane intracellular anatomical structure Golgi apparatus intracellular membrane-bounded organelle endomembrane system intracellular organelle bounding membrane of organelle cell-cell junction adherens junction anchoring junction cell junction cellular anatomical entity membrane membrane-bounded organelle Golgi membrane intracellular anatomical structure Golgi apparatus intracellular membrane-bounded organelle endomembrane system intracellular organelle bounding membrane of organelle organelle cytoplasm organelle membrane cytosol apical junction complex bicellular tight junction tight junction cell periphery plasma membrane growth cone axon cell projection distal axon neuron projection plasma membrane bounded cell projection site of polarized growth

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C [auth B]	IPR045872	ADP-ribosylation factor 1-5	Family
A, C [auth B]	IPR005225	Small GTP-binding protein domain	Domain
A, C [auth B]	IPR006689	Small GTPase superfamily, ARF/SAR type	Family
A, C [auth B]	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
B [auth E], D [auth F]	IPR001849	Pleckstrin homology domain	Domain
B [auth E], D [auth F]	IPR000904	Sec7 domain	Domain
B [auth E], D [auth F]	IPR011993	PH-like domain superfamily	Homologous Superfamily
B [auth E], D [auth F]	IPR023394	Sec7, C-terminal domain superfamily	Homologous Superfamily
B [auth E], D [auth F]	IPR035999	Sec7 domain superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B [auth E], D [auth F]	Pharos: Q99418	adrenal cortex carcinoma diabetes mellitus lung cancer osteosarcoma pancreatic intraductal papillary-mucinous neoplasm atypical teratoid rhabdoid tumor ductal breast carcinoma in situ malignant ependymoma ovarian cancer primitive neuroectodermal tumor breast cancer invasive ductal breast carcinoma medulloblastoma pancreatic intraductal papillary-mucinous carcinoma

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.