Annotations: 1PQ1

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1pq1a_	Membrane and cell surface proteins and peptides	Toxins' membrane translocation domains	Bcl-2 inhibitors of programmed cell death	Bcl-2 inhibitors of programmed cell death	Apoptosis regulator Bcl-xL	house mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
B	d1pq1b_	Peptides	Transmembrane helical fragments	Transmembrane helical fragments	Transmembrane helical fragments	BH3 domain of proapoptotic protein Bim	house mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Bcl-2 inhibitors of programmed cell death	8026680	4002981	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Bcl-2-like inhibitors of programmed cell death	8039059	3001974	SCOP2 (2022-06-29)
B	SCOP2 Family	BH3 domain of BAM Bim and Bcl2-like protein 11	8051435	4007368	SCOP2 (2022-06-29)
B	SCOP2 Superfamily	BH3 domain	8055789	3002075	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Bcl-2	e1pq1A1	A: alpha arrays	X: Toxins' membrane translocation domains	H: Bcl-2 inhibitors of programmed cell death (From Topology)	T: Bcl-2 inhibitors of programmed cell death	F: Bcl-2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.437.10	Mainly Alpha	Orthogonal Bundle	Apoptosis Regulator Bcl-x	Blc2-like	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF02180	Bcl-2 homology region 4 (BH4)	Bcl-2 homology region 4	-	Family
A	PF00452	Apoptosis regulator proteins, Bcl-2 family (Bcl-2)	Apoptosis regulator proteins, Bcl-2 family	-	Family
B	PF08945	Bcl-x interacting, BH3 domain (Bclx_interact)	Bcl-x interacting, BH3 domain	This domain is a long alpha helix, required for interaction with Bcl-x. It is found in BAM, Bim and Bcl2-like protein 11 [1]. This domain is also known as the BH3 domain between residues 146 and 161.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Apoptosis regulator Bcl-X	protein binding binding BH domain binding protein domain specific binding death domain binding BH3 domain binding protein-containing complex binding identical protein binding clathrin binding protein heterodimerization activity protein dimerization activity MDM2/MDM4 family protein binding enzyme binding GTPase binding protein binding binding BH domain binding protein domain specific binding death domain binding BH3 domain binding protein-containing complex binding identical protein binding clathrin binding protein heterodimerization activity protein dimerization activity MDM2/MDM4 family protein binding enzyme binding GTPase binding enzyme regulator activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process molecular function regulator activity cysteine-type endopeptidase regulator activity involved in apoptotic process protein homodimerization activity kinase binding protein kinase binding	developmental process multicellular organismal process gonad development reproductive system development reproductive process sex differentiation animal organ development developmental process involved in reproduction male gonad development male sex differentiation multicellular organism development development of primary male sexual characteristics development of primary sexual characteristics anatomical structure development reproductive structure development developmental process multicellular organismal process gonad development reproductive system development reproductive process sex differentiation animal organ development developmental process involved in reproduction male gonad development male sex differentiation multicellular organism development development of primary male sexual characteristics development of primary sexual characteristics anatomical structure development reproductive structure development system development spermatogenesis sexual reproduction multicellular organismal reproductive process male gamete generation gamete generation cell population proliferation mononuclear cell proliferation dendritic cell proliferation cellular process leukocyte proliferation fertilization response to stimulus response to stress response to ischemia cellular response to stimulus extrinsic apoptotic signaling pathway via death domain receptors regulation of cellular process signaling negative regulation of signaling negative regulation of biological process regulation of signal transduction regulation of programmed cell death extrinsic apoptotic signaling pathway negative regulation of cell communication cell communication negative regulation of extrinsic apoptotic signaling pathway negative regulation of apoptotic signaling pathway negative regulation of cellular process signal transduction regulation of biological process regulation of signaling cell surface receptor signaling pathway negative regulation of response to stimulus negative regulation of programmed cell death regulation of apoptotic process biological regulation negative regulation of extrinsic apoptotic signaling pathway via death domain receptors cell death negative regulation of signal transduction regulation of extrinsic apoptotic signaling pathway apoptotic signaling pathway negative regulation of apoptotic process regulation of response to stimulus regulation of extrinsic apoptotic signaling pathway via death domain receptors regulation of cell communication apoptotic process programmed cell death regulation of apoptotic signaling pathway regulation of growth growth positive regulation of programmed cell death positive regulation of biological process positive regulation of apoptotic process positive regulation of cellular process response to radiation response to abiotic stimulus regulation of anoikis negative regulation of anoikis anoikis cellular response to nitrogen compound response to organic substance response to organonitrogen compound response to endogenous stimulus response to nitrogen compound response to alkaloid cellular response to endogenous stimulus cellular response to chemical stimulus cellular response to organonitrogen compound response to chemical cellular response to organic substance cellular response to alkaloid regulation of localization apoptotic mitochondrial changes localization regulation of biological quality positive regulation of membrane permeability negative regulation of cellular component organization establishment of localization regulation of organelle organization regulation of membrane permeability negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway regulation of mitochondrial membrane permeability involved in apoptotic process regulation of cellular component organization negative regulation of mitochondrial membrane permeability transport mitochondrial outer membrane permeabilization involved in programmed cell death mitochondrial membrane organization cellular component organization positive regulation of mitochondrial membrane permeability involved in apoptotic process positive regulation of mitochondrial membrane permeability cellular component organization or biogenesis regulation of mitochondrial membrane permeability mitochondrial transport mitochondrion organization membrane organization regulation of transport organelle organization negative regulation of organelle organization regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway regulation of mitochondrion organization negative regulation of membrane permeability mitochondrial outer membrane permeabilization embryo development ending in birth or egg hatching chordate embryonic development embryo development in utero embryonic development apoptotic process in bone marrow cell dendritic cell apoptotic process leukocyte apoptotic process signal transduction in absence of ligand extrinsic apoptotic signaling pathway in absence of ligand negative regulation of reproductive process regulation of reproductive process regulation of cytokinesis regulation of cell cycle process cytokinesis regulation of cell division cell division cell cycle regulation of cell cycle cell cycle process female gonad development female sex differentiation development of primary female sexual characteristics ovarian follicle development regulation of release of cytochrome c from mitochondria negative regulation of release of cytochrome c from mitochondria release of cytochrome c from mitochondria cellular response to acid chemical cellular response to amino acid stimulus response to acid chemical response to amino acid regulation of leukocyte proliferation positive regulation of cell population proliferation regulation of cell population proliferation positive regulation of leukocyte proliferation positive regulation of mononuclear cell proliferation regulation of mononuclear cell proliferation cellular developmental process cell development programmed cell death involved in cell development cell differentiation ectopic germ cell programmed cell death positive regulation of cell communication regulated exocytosis positive regulation of neurotransmitter secretion synaptic vesicle cycle synaptic vesicle exocytosis vesicle-mediated transport vesicle-mediated transport in synapse positive regulation of regulated secretory pathway establishment of localization in cell trans-synaptic signaling positive regulation of neurotransmitter transport neurotransmitter secretion regulation of synaptic vesicle exocytosis positive regulation of synaptic transmission neurotransmitter transport regulation of secretion signal release from synapse regulation of trans-synaptic signaling exocytosis cellular localization modulation of chemical synaptic transmission positive regulation of secretion by cell positive regulation of exocytosis regulation of regulated secretory pathway synaptic signaling regulation of secretion by cell secretion by cell positive regulation of secretion signal release positive regulation of synaptic vesicle exocytosis secretion regulation of neurotransmitter transport export from cell cell-cell signaling regulation of vesicle-mediated transport chemical synaptic transmission positive regulation of transport regulation of neurotransmitter secretion anterograde trans-synaptic signaling regulation of exocytosis positive regulation of signaling response to ketone response to cycloheximide response to alcohol response to oxygen-containing compound response to organic cyclic compound nucleotide biosynthetic process organonitrogen compound biosynthetic process carbohydrate derivative metabolic process purine ribonucleotide biosynthetic process positive regulation of phosphate metabolic process purine ribonucleoside triphosphate biosynthetic process positive regulation of nucleotide biosynthetic process regulation of nucleotide biosynthetic process regulation of primary metabolic process regulation of ATP metabolic process nucleobase-containing compound metabolic process positive regulation of metabolic process ribonucleotide biosynthetic process purine nucleoside triphosphate biosynthetic process regulation of small molecule metabolic process positive regulation of purine nucleotide biosynthetic process regulation of phosphate metabolic process regulation of nucleotide metabolic process phosphate-containing compound metabolic process organic substance biosynthetic process small molecule metabolic process regulation of nucleobase-containing compound metabolic process phosphorus metabolic process ribose phosphate biosynthetic process nucleoside phosphate metabolic process positive regulation of nucleobase-containing compound metabolic process positive regulation of phosphorus metabolic process cellular metabolic process primary metabolic process regulation of cellular metabolic process nucleotide metabolic process regulation of purine nucleotide metabolic process ribonucleoside triphosphate biosynthetic process organic cyclic compound metabolic process positive regulation of ATP metabolic process biosynthetic process organophosphate biosynthetic process organic cyclic compound biosynthetic process nucleoside phosphate biosynthetic process metabolic process regulation of phosphorus metabolic process nucleoside triphosphate metabolic process purine ribonucleotide metabolic process regulation of metabolic process purine nucleotide biosynthetic process ribose phosphate metabolic process ATP metabolic process ATP biosynthetic process positive regulation of nucleotide metabolic process positive regulation of cellular metabolic process purine nucleotide metabolic process organic substance metabolic process ribonucleotide metabolic process positive regulation of biosynthetic process nucleobase-containing small molecule metabolic process purine ribonucleoside triphosphate metabolic process purine-containing compound biosynthetic process ribonucleoside triphosphate metabolic process purine nucleoside triphosphate metabolic process regulation of biosynthetic process carbohydrate derivative biosynthetic process regulation of purine nucleotide biosynthetic process organonitrogen compound metabolic process nucleoside triphosphate biosynthetic process nucleobase-containing compound biosynthetic process purine-containing compound metabolic process positive regulation of purine nucleotide metabolic process positive regulation of ATP biosynthetic process regulation of ATP biosynthetic process organophosphate metabolic process positive regulation of small molecule metabolic process negative regulation of intracellular signal transduction regulation of cellular response to stress regulation of intrinsic apoptotic signaling pathway in response to DNA damage regulation of intrinsic apoptotic signaling pathway intracellular signal transduction cellular response to stress regulation of intracellular signal transduction regulation of response to stress intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of intrinsic apoptotic signaling pathway DNA damage response biological process involved in interspecies interaction between organisms response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to virus defense response to other organism response to external biotic stimulus response to virus protein localization regulation of protein localization negative regulation of protein localization to plasma membrane cellular macromolecule localization regulation of protein localization to membrane negative regulation of protein localization protein localization to cell periphery protein localization to plasma membrane localization within membrane regulation of protein localization to cell periphery protein localization to membrane negative regulation of protein localization to membrane regulation of cellular localization macromolecule localization negative regulation of protein localization to cell periphery regulation of protein localization to plasma membrane cellular process involved in reproduction in multicellular organism germ cell development cellular response to ionizing radiation cellular response to radiation cellular response to environmental stimulus response to ionizing radiation cellular response to gamma radiation cellular response to abiotic stimulus response to gamma radiation regulation of mitochondrial membrane potential regulation of membrane potential regulation of response to endoplasmic reticulum stress negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway response to endoplasmic reticulum stress negative regulation of response to endoplasmic reticulum stress vesicle localization synaptic vesicle clustering organelle localization positive regulation of synaptic vesicle clustering regulation of synaptic vesicle clustering synaptic vesicle localization response to cytokine neuron apoptotic process negative regulation of neuron apoptotic process regulation of neuron apoptotic process intracellular transport endosomal transport synaptic vesicle endosomal processing synaptic vesicle recycling via endosome synaptic vesicle recycling negative regulation of execution phase of apoptosis regulation of execution phase of apoptosis execution phase of apoptosis regulation of developmental process negative regulation of developmental process regulation of leukocyte apoptotic process negative regulation of dendritic cell apoptotic process negative regulation of leukocyte apoptotic process regulation of dendritic cell apoptotic process epithelial cell proliferation hepatocyte apoptotic process epithelial cell apoptotic process negative regulation of synaptic transmission regulation of synaptic plasticity long-term synaptic depression regulation of long-term synaptic depression import into cell positive regulation of cellular component organization positive regulation of synaptic vesicle recycling regulation of synaptic vesicle recycling presynaptic endocytosis positive regulation of endocytosis regulation of synaptic vesicle endocytosis positive regulation of synaptic vesicle endocytosis regulation of endocytosis synaptic vesicle endocytosis endocytosis	membrane cellular anatomical entity membrane-bounded organelle intracellular anatomical structure nuclear membrane nucleus intracellular membrane-bounded organelle endomembrane system intracellular organelle organelle envelope organelle nuclear envelope organelle membrane mitochondrial membrane membrane cellular anatomical entity membrane-bounded organelle intracellular anatomical structure nuclear membrane nucleus intracellular membrane-bounded organelle endomembrane system intracellular organelle organelle envelope organelle nuclear envelope organelle membrane mitochondrial membrane mitochondrion mitochondrial envelope cytoplasm cytosol organelle outer membrane bounding membrane of organelle mitochondrial outer membrane outer membrane synapse intracellular vesicle transport vesicle membrane presynapse cytoplasmic vesicle vesicle membrane synaptic vesicle synaptic vesicle membrane cell junction vesicle transport vesicle exocytic vesicle membrane cytoplasmic vesicle membrane secretory vesicle exocytic vesicle organelle inner membrane mitochondrial inner membrane centrosome microtubule organizing center cytoskeleton intracellular non-membrane-bounded organelle microtubule cytoskeleton non-membrane-bounded organelle protein-containing complex Bcl-2 family protein complex membrane-enclosed lumen mitochondrial matrix intracellular organelle lumen organelle lumen endoplasmic reticulum
B	BCL2-like protein 11	cytoskeletal protein binding microtubule binding protein binding binding tubulin binding molecular adaptor activity kinase binding enzyme binding protein kinase binding	developmental process multicellular organismal process gonad development reproductive system development reproductive process sex differentiation animal organ development developmental process involved in reproduction male gonad development male sex differentiation multicellular organism development development of primary male sexual characteristics development of primary sexual characteristics anatomical structure development reproductive structure development developmental process multicellular organismal process gonad development reproductive system development reproductive process sex differentiation animal organ development developmental process involved in reproduction male gonad development male sex differentiation multicellular organism development development of primary male sexual characteristics development of primary sexual characteristics anatomical structure development reproductive structure development system development spermatogenesis sexual reproduction multicellular organismal reproductive process male gamete generation gamete generation cellular response to stimulus positive regulation of organelle organization regulation of cellular process regulation of cellular component organization positive regulation of cellular component organization response to stimulus signaling apoptotic mitochondrial changes positive regulation of biological process regulation of release of cytochrome c from mitochondria cell communication cellular component organization positive regulation of release of cytochrome c from mitochondria cellular component organization or biogenesis signal transduction positive regulation of cellular process mitochondrion organization regulation of biological process biological regulation cell death regulation of organelle organization apoptotic signaling pathway release of cytochrome c from mitochondria organelle organization regulation of mitochondrion organization cellular process apoptotic process programmed cell death protein-containing complex organization positive regulation of cellular component biogenesis regulation of cellular component biogenesis cellular component biogenesis positive regulation of protein-containing complex assembly regulation of protein-containing complex assembly cellular component assembly protein-containing complex assembly positive regulation of programmed cell death regulation of apoptotic process regulation of programmed cell death positive regulation of apoptotic process regulation of leukocyte apoptotic process regulation of lymphocyte apoptotic process positive regulation of lymphocyte apoptotic process leukocyte apoptotic process lymphocyte apoptotic process positive regulation of leukocyte apoptotic process multicellular organismal-level homeostasis leukocyte homeostasis homeostatic process immune system process homeostasis of number of cells neuron apoptotic process positive regulation of neuron apoptotic process regulation of neuron apoptotic process B cell homeostasis lymphocyte homeostasis mammary gland development gland development embryo development ending in birth or egg hatching chordate embryonic development embryo development in utero embryonic development pigmentation developmental pigmentation signal transduction in absence of ligand cell surface receptor signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand fibroblast apoptotic process positive regulation of fibroblast apoptotic process regulation of fibroblast apoptotic process immune system development spleen development hematopoietic or lymphoid organ development positive regulation of cell communication positive regulation of signal transduction regulation of signal transduction regulation of intrinsic apoptotic signaling pathway intracellular signal transduction positive regulation of apoptotic signaling pathway positive regulation of response to stimulus regulation of intracellular signal transduction intrinsic apoptotic signaling pathway regulation of signaling positive regulation of intracellular signal transduction regulation of response to stimulus regulation of cell communication positive regulation of intrinsic apoptotic signaling pathway regulation of apoptotic signaling pathway positive regulation of signaling positive regulation of T cell apoptotic process regulation of T cell apoptotic process T cell apoptotic process thymocyte apoptotic process kidney development renal system development cellular response to stress response to stress response to endoplasmic reticulum stress cell cycle regulation of cell cycle positive regulation of cell cycle apoptotic process involved in morphogenesis limb development appendage development embryonic limb morphogenesis embryonic morphogenesis embryonic appendage morphogenesis apoptotic process involved in development anatomical structure morphogenesis apoptotic process involved in embryonic digit morphogenesis appendage morphogenesis embryonic digit morphogenesis limb morphogenesis cell adhesion cell-matrix adhesion cell-substrate adhesion thymus development ear development sensory organ development odontogenesis of dentin-containing tooth odontogenesis animal organ morphogenesis meiotic cell cycle organelle fission meiotic cell cycle process nuclear division meiosis I meiosis I cell cycle process meiotic nuclear division cell cycle process regulation of developmental pigmentation regulation of pigmentation T cell homeostasis regulation of growth regulation of developmental growth regulation of developmental process developmental growth growth organ growth regulation of organ growth regulation of multicellular organismal process myeloid cell homeostasis DNA damage response intrinsic apoptotic signaling pathway in response to DNA damage catabolic process autophagy positive regulation of cellular metabolic process regulation of autophagy response to biotic stimulus cellular catabolic process cellular response to biotic stimulus positive regulation of metabolic process positive regulation of autophagy in response to ER overload ER-nucleus signaling pathway process utilizing autophagic mechanism positive regulation of autophagy cellular metabolic process regulation of cellular metabolic process regulation of catabolic process positive regulation of catabolic process ER overload response regulation of cellular catabolic process positive regulation of cellular catabolic process metabolic process regulation of metabolic process transport mitochondrial outer membrane permeabilization involved in programmed cell death mitochondrial membrane organization localization positive regulation of mitochondrial membrane permeability involved in apoptotic process positive regulation of mitochondrial membrane permeability positive regulation of membrane permeability regulation of biological quality regulation of mitochondrial membrane permeability mitochondrial transport membrane organization establishment of localization regulation of membrane permeability regulation of mitochondrial membrane permeability involved in apoptotic process post-embryonic development tube morphogenesis tube development anatomical structure formation involved in morphogenesis tube formation	protein-containing complex BIM-BCL-2 complex Bcl-2 family protein complex membrane cellular anatomical entity intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle mitochondrion organelle intracellular membrane-bounded organelle BIM-BCL-xl complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR036834	Bcl-2-like superfamily	Homologous Superfamily
A	IPR004725	Apoptosis regulator, Bcl-2/ BclX	Family
A	IPR003093	Apoptosis regulator, Bcl-2 protein, BH4	Domain
A	IPR002475	Bcl2-like	Family
A	IPR020731	Apoptosis regulator, Bcl-2, BH4 motif, conserved site	Conserved Site
A	IPR013279	Apoptosis regulator, Bcl-X	Family
A	IPR020728	Apoptosis regulator, Bcl-2, BH3 motif, conserved site	Conserved Site
A	IPR020726	Apoptosis regulator, Bcl-2, BH2 motif, conserved site	Conserved Site
A	IPR046371	Bcl-2, Bcl-2 homology region 1-3	Domain
A	IPR026298	Bcl-2 family	Family
A	IPR020717	Apoptosis regulator, Bcl-2, BH1 motif, conserved site	Conserved Site
B	IPR014771	Apoptosis, Bim N-terminal	Domain
B	IPR017288	Bcl-2-like protein 11	Family
B	IPR015040	Bcl-x interacting, BH3 domain	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.