1OTG

5-CARBOXYMETHYL-2-HYDROXYMUCONATE ISOMERASE

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1otga_	Alpha and beta proteins (a+b)	Tautomerase/MIF	Tautomerase/MIF	5-carboxymethyl-2-hydroxymuconate isomerase (CHMI)	5-carboxymethyl-2-hydroxymuconate isomerase (CHMI)	(Escherichia coli C ) [TaxId: 498388 ],	SCOPe (2.08)
B	d1otgb_	Alpha and beta proteins (a+b)	Tautomerase/MIF	Tautomerase/MIF	5-carboxymethyl-2-hydroxymuconate isomerase (CHMI)	5-carboxymethyl-2-hydroxymuconate isomerase (CHMI)	(Escherichia coli C ) [TaxId: 498388 ],	SCOPe (2.08)
C	d1otgc_	Alpha and beta proteins (a+b)	Tautomerase/MIF	Tautomerase/MIF	5-carboxymethyl-2-hydroxymuconate isomerase (CHMI)	5-carboxymethyl-2-hydroxymuconate isomerase (CHMI)	(Escherichia coli C ) [TaxId: 498388 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	5-carboxymethyl-2-hydroxymuconate isomerase (CHMI)	8023674	4001529	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Tautomerase/MIF	8036054	3001770	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Tautomerase/MIF	8036054	3001770	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Tautomerase/MIF	8036054	3001770	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF02962	e1otgA1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
B	PF02962	e1otgB1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)
C	PF02962	e1otgC1	A: a+b two layers	X: Tautomerase/MIF-like	H: Tautomerase/MIF (From Topology)	T: Tautomerase/MIF	F: PF02962	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
B	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)
C	3.30.429.10	Alpha Beta	2-Layer Sandwich	Macrophage Migration Inhibitory Factor	Macrophage Migration Inhibitory Factor	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C	PF02962	5-carboxymethyl-2-hydroxymuconate isomerase (CHMI)	5-carboxymethyl-2-hydroxymuconate isomerase	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C	5-CARBOXYMETHYL-2-HYDROXYMUCONATE ISOMERASE	isomerase activity intramolecular oxidoreductase activity, transposing C=C bonds intramolecular oxidoreductase activity 5-carboxymethyl-2-hydroxymuconate delta-isomerase activity catalytic activity	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C	IPR014347	Tautomerase/MIF superfamily	Homologous Superfamily
A, B, C	IPR004220	5-carboxymethyl-2-hydroxymuconate isomerase	Family

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	5-carboxymethyl-2-hydroxymuconate Delta-isomerase M-CSA #874	5-carboxymethyl-2-hydroxymuconate isomerase (CHMI) transforms 5-carboxymethyl-2-hydroxy-muconic acid into 5-oxo-pent-3-ene-1,2,5-tricarboxylic acid during the third step of the homoprotocatechuate catabolic pathway [PMID:2194841]. CHMI is a member of the tautomerase superfamily many of which utilise a unique N-terminal proline residue as a general acid/base.	Defined by 4 residues: PRO:A-1 [auth A-2]PRO:A-35 [auth A-36]ARG:A-40 [auth A-41]ARG:A-71 [auth A-72] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 5.3.2 (PDB Primary Data) Search M-CSA Motif + EC 5.3.2

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.