1OR7

Crystal Structure of Escherichia coli sigmaE with the Cytoplasmic Domain of its Anti-sigma RseA

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C [auth B]	d1or7b1	All alpha proteins	DNA/RNA-binding 3-helical bundle	Sigma3 and sigma4 domains of RNA polymerase sigma factors	Sigma4 domain	SigmaE factor (RpoE)	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C [auth B]	d1or7b2	All alpha proteins	Sigma2 domain of RNA polymerase sigma factors	Sigma2 domain of RNA polymerase sigma factors	Sigma2 domain of RNA polymerase sigma factors	SigmaE factor (RpoE)	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
C [auth B]	d1or7b3	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d1or7a1	All alpha proteins	DNA/RNA-binding 3-helical bundle	Sigma3 and sigma4 domains of RNA polymerase sigma factors	Sigma4 domain	SigmaE factor (RpoE)	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d1or7a2	All alpha proteins	Sigma2 domain of RNA polymerase sigma factors	Sigma2 domain of RNA polymerase sigma factors	Sigma2 domain of RNA polymerase sigma factors	SigmaE factor (RpoE)	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
A	d1or7a3	Artifacts	Tags	Tags	Tags	N-terminal Tags	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
B [auth C]	d1or7c_	All alpha proteins	N-terminal, cytoplasmic domain of anti-sigmaE factor RseA	N-terminal, cytoplasmic domain of anti-sigmaE factor RseA	N-terminal, cytoplasmic domain of anti-sigmaE factor RseA	N-terminal, cytoplasmic domain of anti-sigmaE factor RseA	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)
D [auth F]	d1or7f_	All alpha proteins	N-terminal, cytoplasmic domain of anti-sigmaE factor RseA	N-terminal, cytoplasmic domain of anti-sigmaE factor RseA	N-terminal, cytoplasmic domain of anti-sigmaE factor RseA	N-terminal, cytoplasmic domain of anti-sigmaE factor RseA	(Escherichia coli ) [TaxId: 562 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C [auth B]	SCOP2B Superfamily	Sigma factor domain-like	8034262	3000953	SCOP2B (2022-06-29)
C [auth B]	SCOP2B Superfamily	Sigma2 domain of RNA polymerase sigma factors	8034263	3001393	SCOP2B (2022-06-29)
A	SCOP2 Family	Sigma2 domain of RNA polymerase sigma factors	8021883	4003741	SCOP2 (2022-06-29)
A	SCOP2 Family	Sigma4 domain	8021882	4003503	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Sigma2 domain of RNA polymerase sigma factors	8034263	3001393	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Sigma factor domain-like	8034262	3000953	SCOP2 (2022-06-29)
B [auth C]	SCOP2 Family	N-terminal cytoplasmic domain of anti-sigmaE factor RseA	8021884	4003757	SCOP2 (2022-06-29)
B [auth C]	SCOP2 Superfamily	N-terminal cytoplasmic domain of anti-sigmaE factor RseA-like	8034264	3001438	SCOP2 (2022-06-29)
D [auth F]	SCOP2B Superfamily	N-terminal cytoplasmic domain of anti-sigmaE factor RseA-like	8034264	3001438	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C [auth B]	PF08281	e1or7B1	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: PF08281	ECOD (1.6)
C [auth B]	PF04542	e1or7B2	A: alpha complex topology	X: Sigma2 domain-like	H: Sigma2 domain of RNA polymerase sigma factors (From Topology)	T: Sigma2 domain of RNA polymerase sigma factors	F: PF04542	ECOD (1.6)
A	PF08281	e1or7A1	A: alpha arrays	X: HTH	H: HTH	T: tri-helical	F: PF08281	ECOD (1.6)
A	PF04542	e1or7A2	A: alpha complex topology	X: Sigma2 domain-like	H: Sigma2 domain of RNA polymerase sigma factors (From Topology)	T: Sigma2 domain of RNA polymerase sigma factors	F: PF04542	ECOD (1.6)
B [auth C]	PF03872	e1or7C1	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: PF03872	ECOD (1.6)
D [auth F]	PF03872	e1or7F1	A: alpha arrays	X: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	H: N-terminal, cytoplasmic domain of anti-sigma factors (From Topology)	T: N-terminal, cytoplasmic domain of anti-sigma factors	F: PF03872	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C [auth B]	1.10.1740.10	Mainly Alpha	Orthogonal Bundle	Rna Polymerase Sigma Factor	Chain: A	CATH (4.3.0)
C [auth B]	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
A	1.10.1740.10	Mainly Alpha	Orthogonal Bundle	Rna Polymerase Sigma Factor	Chain: A	CATH (4.3.0)
A	1.10.10.10	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain	CATH (4.3.0)
B [auth C]	1.10.10.880	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti sigma-E protein RseA, N-terminal domain	CATH (4.3.0)
D [auth F]	1.10.10.880	Mainly Alpha	Orthogonal Bundle	Arc Repressor Mutant, subunit A	Anti sigma-E protein RseA, N-terminal domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C [auth B]	PF08281	Sigma-70, region 4 (Sigma70_r4_2)	Sigma-70, region 4	Region 4 of sigma-70 like sigma-factors are involved in binding to the -35 promoter element via a helix-turn-helix motif [1].	Domain
A, C [auth B]	PF04542	Sigma-70 region 2 (Sigma70_r2)	Sigma-70 region 2	Region 2 of sigma-70 is the most conserved region of the entire protein. All members of this class of sigma-factor contain region 2. The high conservation is due to region 2 containing both the -10 promoter recognition helix and the primary core RNA ...	Region 2 of sigma-70 is the most conserved region of the entire protein. All members of this class of sigma-factor contain region 2. The high conservation is due to region 2 containing both the -10 promoter recognition helix and the primary core RNA polymerase binding determinant. The core binding helix, interacts with the clamp domain of the largest polymerase subunit, beta prime [1,2]. The aromatic residues of the recognition helix, found at the C-terminus of this domain are though to mediate strand separation, thereby allowing transcription initiation [1,2].	Domain
B [auth C], D [auth F]	PF03872	Anti sigma-E protein RseA, N-terminal domain (RseA_N)	Anti sigma-E protein RseA, N-terminal domain	Sigma-E is important for the induction of proteins involved in heat shock response. RseA binds sigma-E via its N-terminal domain, sequestering sigma-E and preventing transcription from heat-shock promoters [1]. The C-terminal domain is located in t ...	Sigma-E is important for the induction of proteins involved in heat shock response. RseA binds sigma-E via its N-terminal domain, sequestering sigma-E and preventing transcription from heat-shock promoters [1]. The C-terminal domain is located in the periplasm, and may interact with other protein that signal periplasmic stress.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C [auth B]	RNA polymerase sigma-E factor	DNA binding binding organic cyclic compound binding nucleic acid binding transcription regulator activity DNA-binding transcription factor activity sigma factor activity	regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process negative regulation of metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process organic substance metabolic process negative regulation of cellular process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process biological regulation regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process cellular process regulation of DNA-templated transcription initiation DNA-templated transcription initiation cellular response to stimulus cellular response to stress response to stimulus response to stress cellular response to cell envelope stress response to osmotic stress response to abiotic stimulus cell aggregation biofilm formation aggregation of unicellular organisms submerged biofilm formation response to temperature stimulus	protein-containing complex sigma factor antagonist complex transcription regulator complex intracellular anatomical structure catalytic complex transferase complex cellular anatomical entity transferase complex, transferring phosphorus-containing groups cytosolic DNA-directed RNA polymerase complex RNA polymerase complex DNA-directed RNA polymerase complex cytoplasm cytosol intracellular protein-containing complex protein-containing complex sigma factor antagonist complex transcription regulator complex intracellular anatomical structure catalytic complex transferase complex cellular anatomical entity transferase complex, transferring phosphorus-containing groups cytosolic DNA-directed RNA polymerase complex RNA polymerase complex DNA-directed RNA polymerase complex cytoplasm cytosol intracellular protein-containing complex cell periphery plasma membrane membrane
B [auth C], D [auth F]	Sigma-E factor negative regulatory protein	molecular adaptor activity sigma factor antagonist activity transcription corepressor activity transcription regulator activity transcription coregulator activity protein-macromolecule adaptor activity	regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process regulation of cellular process negative regulation of biological process negative regulation of cellular metabolic process regulation of primary metabolic process negative regulation of RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription regulation of RNA metabolic process organic substance biosynthetic process negative regulation of nucleobase-containing compound metabolic process regulation of nucleobase-containing compound metabolic process negative regulation of DNA-templated transcription regulation of biological process cellular metabolic process negative regulation of metabolic process primary metabolic process regulation of cellular metabolic process negative regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process negative regulation of macromolecule biosynthetic process organic cyclic compound biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription regulation of metabolic process negative regulation of RNA metabolic process cellular biosynthetic process RNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process organic substance metabolic process negative regulation of cellular process regulation of RNA biosynthetic process negative regulation of macromolecule metabolic process regulation of biosynthetic process biological regulation regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process negative regulation of biosynthetic process cellular process	protein-containing complex sigma factor antagonist complex transcription regulator complex cell periphery plasma membrane membrane cellular anatomical entity

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C [auth B]	IPR007627	RNA polymerase sigma-70 region 2	Domain
A, C [auth B]	IPR013325	RNA polymerase sigma factor, region 2	Homologous Superfamily
A, C [auth B]	IPR013324	RNA polymerase sigma factor, region 3/4-like	Homologous Superfamily
A, C [auth B]	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
A, C [auth B]	IPR000838	RNA polymerase sigma factor 70, ECF, conserved site	Conserved Site
A, C [auth B]	IPR014284	RNA polymerase sigma-70 like domain	Domain
A, C [auth B]	IPR039425	RNA polymerase sigma-70 like	Family
A, C [auth B]	IPR013249	RNA polymerase sigma factor 70, region 4 type 2	Domain
A, C [auth B]	IPR014286	RNA polymerase sigma-70 RpoE type	Family
B [auth C], D [auth F]	IPR005573	Anti sigma-E protein RseA, C-terminal	Domain
B [auth C], D [auth F]	IPR036147	Anti sigma-E protein RseA, N-terminal domain superfamily	Homologous Superfamily
B [auth C], D [auth F]	IPR026279	Sigma-E factor negative regulatory protein RseA	Family
B [auth C], D [auth F]	IPR005572	Anti sigma-E protein RseA, N-terminal	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.