1MHT

COVALENT TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE, DNA AND S-ADENOSYL-L-HOMOCYSTEINE

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C [auth A]	d1mhta_	Alpha and beta proteins (a/b)	S-adenosyl-L-methionine-dependent methyltransferases	S-adenosyl-L-methionine-dependent methyltransferases	C5 cytosine-specific DNA methylase, DCM	DNA methylase HhaI	(Haemophilus haemolyticus ) [TaxId: 726 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
C [auth A]	SCOP2B Superfamily	S-adenosyl-L-methionine-dependent methyltransferases	8038508	3000118	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C [auth A]	DNA_methylase	e1mhtA1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: DNA_methylase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C [auth A]	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
C [auth A]	3.90.120.10	Alpha Beta	Alpha-Beta Complex	DNA Methylase	Chain A, domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
C [auth A]	PF00145	C-5 cytosine-specific DNA methylase (DNA_methylase)	C-5 cytosine-specific DNA methylase	-	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B]	DNA (5'-D(PGPAPTPAPGP(C36)PGPCPTPAPTPC)-3')	-	-	-
B [auth C]	DNA (5'-D(TPGPAPTPAPGP(C36)PGPCPTPAPTP*C)-3')	-	-	-
C [auth A]	PROTEIN (HHAI METHYLTRANSFERASE)	DNA binding binding organic cyclic compound binding nucleic acid binding DNA (cytosine-5-)-methyltransferase activity DNA-methyltransferase activity catalytic activity, acting on DNA transferase activity, transferring one-carbon groups catalytic activity, acting on a nucleic acid transferase activity methyltransferase activity S-adenosylmethionine-dependent methyltransferase activity catalytic activity	biological process involved in interspecies interaction between organisms response to stimulus DNA restriction-modification system clearance of foreign intracellular nucleic acids response to biotic stimulus nucleobase-containing compound metabolic process DNA modification organic substance metabolic process response to stress response to external biotic stimulus response to other organism defense response defense response to symbiont response to external stimulus biological process involved in interspecies interaction between organisms response to stimulus DNA restriction-modification system clearance of foreign intracellular nucleic acids response to biotic stimulus nucleobase-containing compound metabolic process DNA modification organic substance metabolic process response to stress response to external biotic stimulus response to other organism defense response defense response to symbiont response to external stimulus primary metabolic process clearance of foreign intracellular DNA DNA metabolic process nucleic acid metabolic process organic cyclic compound metabolic process defense response to other organism macromolecule modification metabolic process macromolecule metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
C [auth A]	IPR031303	DNA methylase, C-5 cytosine-specific, conserved site	Conserved Site
C [auth A]	IPR018117	DNA methylase, C-5 cytosine-specific, active site	Active Site
C [auth A]	IPR001525	C-5 cytosine methyltransferase	Family
C [auth A]	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase superfamily	Homologous Superfamily

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
C [auth A]	DNA (cytosine-5-)-methyltransferase (HhaI) M-CSA #293	Methyl transferase (MTase) M. Hhal is an m5C-MTase that forms part of a type II restriction-modification system from Haemophilus haemolyticus. The enzyme recognises the specific tetranucleotide sequence, 5-GCGC-3. DNA methylation is found in diverse organisms ranging from bacteria to mammals and plants. Cytosine-5-methyltransferases (m5CMTases) are involved in a variety of biological processes in prokaryotes and eukaryotes by catalysing the transfer of a methyl group from S-adenosyl- L-methionine (AdoMet) to the C5 position of cytosine. MTases exist as a component of restriction and modification systems in bacteria.	Defined by 4 residues: CYS:C-81 [auth A-81]GLU:C-119 [auth A-119]ARG:C-163 [auth A-163]ARG:C-165 [auth A-165] \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 2.1.1.73 (PDB Primary Data) Search M-CSA Motif + EC 2.1.1.73

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.