1LJ2

Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1lj2a_	Coiled coil proteins	Parallel coiled-coil	Rotavirus nonstructural proteins	NSP3 C-terminal domain, NS34	NSP3 C-terminal domain, NS34	(Simian rotavirus A/SA11 ) [TaxId: 10923 ],	SCOPe (2.08)
B	d1lj2b_	Coiled coil proteins	Parallel coiled-coil	Rotavirus nonstructural proteins	NSP3 C-terminal domain, NS34	NSP3 C-terminal domain, NS34	(Simian rotavirus A/SA11 ) [TaxId: 10923 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Rotavirus NSP3 C-terminal domain NS34	8028925	4002960	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Rotavirus nonstructural proteins	8041304	3001522	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Rotavirus nonstructural proteins	8041304	3001522	SCOP2B (2022-06-29)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.5.970	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces	Nonstructural RNA-binding protein	CATH (4.3.0)
B	1.20.5.970	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces	Nonstructural RNA-binding protein	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF01665	Rotavirus non-structural protein NSP3 (Rota_NSP3)	Rotavirus non-structural protein NSP3	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	NONSTRUCTURAL RNA-BINDING PROTEIN 34	RNA binding binding organic cyclic compound binding nucleic acid binding	regulation of cellular process cellular biosynthetic process organonitrogen compound biosynthetic process regulation of primary metabolic process post-transcriptional regulation of gene expression organic substance metabolic process organic substance biosynthetic process protein metabolic process regulation of biosynthetic process regulation of biological process organonitrogen compound metabolic process cellular metabolic process regulation of protein metabolic process biological regulation regulation of cellular process cellular biosynthetic process organonitrogen compound biosynthetic process regulation of primary metabolic process post-transcriptional regulation of gene expression organic substance metabolic process organic substance biosynthetic process protein metabolic process regulation of biosynthetic process regulation of biological process organonitrogen compound metabolic process cellular metabolic process regulation of protein metabolic process biological regulation regulation of cellular metabolic process primary metabolic process regulation of macromolecule metabolic process gene expression translation macromolecule biosynthetic process regulation of cellular biosynthetic process regulation of gene expression biosynthetic process regulation of translation regulation of macromolecule biosynthetic process metabolic process cellular process macromolecule metabolic process regulation of metabolic process	host cellular component host intracellular part host cell cytoplasm host cell part host intracellular region cellular anatomical entity
C, D	eukaryotic protein synthesis initiation factor	translation regulator activity binding organic cyclic compound binding translation regulator activity, nucleic acid binding nucleic acid binding translation initiation factor activity translation factor activity, RNA binding eukaryotic initiation factor 4E binding protein binding translation initiation factor binding RNA binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding translation regulator activity binding organic cyclic compound binding translation regulator activity, nucleic acid binding nucleic acid binding translation initiation factor activity translation factor activity, RNA binding eukaryotic initiation factor 4E binding protein binding translation initiation factor binding RNA binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding mRNA binding molecular adaptor activity identical protein binding	regulation of biological process regulation of growth regulation of cellular process regulation of cellular component organization positive regulation of biological process biological regulation growth positive regulation of cell growth regulation of cell growth cell growth cellular component organization positive regulation of growth cellular process cellular component organization or biogenesis regulation of biological process regulation of growth regulation of cellular process regulation of cellular component organization positive regulation of biological process biological regulation growth positive regulation of cell growth regulation of cell growth cell growth cellular component organization positive regulation of growth cellular process cellular component organization or biogenesis positive regulation of cellular process organonitrogen compound metabolic process cellular biosynthetic process cellular metabolic process organonitrogen compound biosynthetic process primary metabolic process translation gene expression macromolecule biosynthetic process organic substance metabolic process biosynthetic process organic substance biosynthetic process translational initiation metabolic process protein metabolic process macromolecule metabolic process positive regulation of protein metabolic process regulation of protein metabolic process regulation of macromolecule metabolic process regulation of primary metabolic process positive regulation of metabolic process positive regulation of macromolecule metabolic process regulation of metabolic process positive regulation of cellular component organization protein-containing complex organization positive regulation of cellular component biogenesis eukaryotic translation initiation factor 4F complex assembly regulation of cellular component biogenesis regulation of eukaryotic translation initiation factor 4F complex assembly cellular component biogenesis regulation of protein-containing complex assembly positive regulation of protein-containing complex assembly cellular component assembly positive regulation of eukaryotic translation initiation factor 4F complex assembly protein-containing complex assembly cellular response to stimulus response to stimulus positive regulation of macromolecule biosynthetic process post-transcriptional regulation of gene expression positive regulation of translation in response to stress response to endoplasmic reticulum stress positive regulation of translation in response to endoplasmic reticulum stress regulation of cellular metabolic process regulation of gene expression regulation of macromolecule biosynthetic process positive regulation of translation positive regulation of cellular metabolic process regulation of translation in response to stress cellular response to stress positive regulation of biosynthetic process response to stress regulation of biosynthetic process regulation of translation in response to endoplasmic reticulum stress regulation of cellular biosynthetic process positive regulation of gene expression regulation of translation positive regulation of cellular biosynthetic process regulation of protein modification process regulation of protein phosphorylation negative regulation of biological process regulation of peptidyl-threonine phosphorylation negative regulation of phosphate metabolic process negative regulation of cellular metabolic process regulation of phosphate metabolic process phosphate-containing compound metabolic process negative regulation of protein modification process negative regulation of cellular process peptidyl-amino acid modification peptidyl-threonine modification protein modification process phosphorus metabolic process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process protein phosphorylation phosphorylation negative regulation of metabolic process negative regulation of protein phosphorylation macromolecule modification negative regulation of peptidyl-threonine phosphorylation negative regulation of phosphorus metabolic process regulation of phosphorylation regulation of phosphorus metabolic process negative regulation of phosphorylation peptidyl-threonine phosphorylation cap-dependent translational initiation cytoplasmic translation cytoplasmic translational initiation cellular response to nutrient levels response to nutrient levels multicellular organismal process regulation of presynapse assembly multicellular organism development regulation of presynapse organization cell junction assembly anatomical structure development regulation of synapse structure or activity synapse organization regulation of biological quality regulation of synapse assembly system development developmental process presynapse organization nervous system development regulation of cell junction assembly regulation of synapse organization cell junction organization synapse assembly presynapse assembly tube development animal organ development respiratory tube development lung development respiratory system development multicellular organismal-level homeostasis homeostatic process energy homeostasis positive regulation of RNA binding positive regulation of mRNA cap binding regulation of binding regulation of mRNA binding positive regulation of binding regulation of RNA binding positive regulation of mRNA binding regulation of molecular function positive regulation of molecular function regulation of mRNA cap binding positive regulation of phosphorylation regulation of peptidyl-serine phosphorylation positive regulation of phosphate metabolic process peptidyl-serine modification positive regulation of protein modification process peptidyl-serine phosphorylation positive regulation of protein phosphorylation positive regulation of phosphorus metabolic process positive regulation of peptidyl-serine phosphorylation negative regulation of translation miRNA-mediated gene silencing by inhibition of translation regulatory ncRNA-mediated post-transcriptional gene silencing negative regulation of cellular biosynthetic process regulatory ncRNA-mediated gene silencing miRNA-mediated post-transcriptional gene silencing negative regulation of gene expression negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process post-transcriptional gene silencing positive regulation of protein localization to cell periphery protein localization regulation of localization cellular localization protein localization to cell periphery regulation of protein localization regulation of protein localization to cell periphery localization regulation of cellular localization cellular macromolecule localization macromolecule localization positive regulation of protein localization regulation of cellular response to stress regulation of response to stimulus regulation of response to stress cellular developmental process generation of neurons neurogenesis neuron differentiation cell differentiation regulation of cell cycle phase transition cell cycle phase transition regulation of cell cycle G1/S phase transition cell cycle G1/S phase transition mitotic cell cycle regulation of mitotic cell cycle positive regulation of mitotic cell cycle phase transition positive regulation of cell cycle process positive regulation of mitotic cell cycle cell cycle regulation of cell cycle cell cycle process positive regulation of G1/S transition of mitotic cell cycle regulation of cell cycle process positive regulation of cell cycle regulation of mitotic cell cycle phase transition positive regulation of cell cycle G1/S phase transition mitotic cell cycle process regulation of G1/S transition of mitotic cell cycle positive regulation of cell cycle phase transition G1/S transition of mitotic cell cycle mitotic cell cycle phase transition response to organic substance response to alcohol response to chemical response to oxygen-containing compound response to ethanol regulation of developmental process regulation of cell differentiation positive regulation of neuron differentiation positive regulation of developmental process positive regulation of cell differentiation regulation of neuron differentiation regulation of translational initiation negative regulation of cellular catabolic process negative regulation of catabolic process catabolic process autophagy regulation of catabolic process regulation of autophagy cellular catabolic process negative regulation of autophagy regulation of cellular catabolic process process utilizing autophagic mechanism behavioral defense response behavior fear response behavioral fear response defense response multicellular organismal response to stress	intracellular anatomical structure cytoplasmic ribonucleoprotein granule cellular anatomical entity ribonucleoprotein granule intracellular organelle organelle intracellular non-membrane-bounded organelle cytoplasm supramolecular complex non-membrane-bounded organelle cytoplasmic stress granule membrane cytosol ribosome intracellular anatomical structure cytoplasmic ribonucleoprotein granule cellular anatomical entity ribonucleoprotein granule intracellular organelle organelle intracellular non-membrane-bounded organelle cytoplasm supramolecular complex non-membrane-bounded organelle cytoplasmic stress granule membrane cytosol ribosome protein-containing complex eukaryotic translation initiation factor 4F complex RNA cap binding complex intracellular protein-containing complex postsynapse synapse cell junction membrane-bounded organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR042519	Non-structural protein NSP3, N-terminal, rotavirus	Homologous Superfamily
A, B	IPR002873	Rotavirus non-structural protein NSP3	Family
A, B	IPR036082	NSP3 superfamily	Homologous Superfamily
C, D	IPR016024	Armadillo-type fold	Homologous Superfamily
C, D	IPR003307	W2 domain	Domain
C, D	IPR003891	Initiation factor eIF-4 gamma, MA3	Domain
C, D	IPR045208	Initiation factor 4G	Family
C, D	IPR003890	MIF4G-like, type 3	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
C, D	Pharos: Q04637	Parkinson disease 18, autosomal dominant, susceptibility to Parkinson disease adenoma Autosomal dominant late onset Parkinson disease glioblastoma neurodegenerative disease amyotrophic lateral sclerosis colorectal cancer intellectual disability non-small cell lung carcinoma astrocytic tumor gastric carcinoma large cell medulloblastoma ovarian cancer

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.